Cortactin (CTTN) (NM_005231) Human Untagged Clone

CAT#: SC313961

CTTN (untagged)-Human cortactin (CTTN), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Cortactin
• Synonyms: EMS1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC313961 representing NM_005231.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGTGGAAAGCTTCAGCAGGCCACGCTGTGTCCATCGCCCAGGATGACGCGGGGGCCGATGACTGGGAG
  ACCGACCCTGATTTTGTGAATGATGTGAGTGAGAAGGAGCAAAGATGGGGTGCCAAGACGGTGCAGGGC
  TCCGGGCACCAGGAGCATATCAACATACACAAGCTGAGGGAGAATGTCTTTCAAGAGCATCAGACCCTT
  AAGGAGAAGGAACTTGAAACAGGACCAAAAGCTTCCCATGGCTATGGAGGGAAATTTGGTGTGGAACAA
  GACCGAATGGATAAGTCAGCTGTCGGCCACGAATATCAGTCGAAACTTTCCAAGCACTGCTCGCAGGTG
  GACTCGGTCCGTGGCTTCGGAGGCAAGTTTGGTGTCCAGATGGACAGAGTTGATCAGTCTGCTGTAGGC
  TTTGAATACCAGGGGAAGACTGAGAAGCATGCCTCCCAGAAAGACTACTCCAGTGGTTTTGGCGGCAAG
  TATGGCGTGCAGGCCGACCGAGTAGACAAGAGCGCGGTGGGCTTCGACTACCAGGGCAAGACGGAGAAG
  CACGAGTCACAGAGAGATTACTCCAAAGGTTTCGGCGGCAAATACGGTATCGACAAGGACAAAGTGGAT
  AAGAGCGCCGTTGGCTTTGAGTATCAAGGCAAAACGGAGAAGCACGAGTCCCAGAAAGACTATGTGAAA
  GGGTTTGGAGGAAAATTTGGTGTGCAGACAGACAGACAAGACAAATGTGCCCTTGGCTGGGATCACCAG
  GAGAAATTGCAGCTGCATGAATCCCAAAAAGATTATAAGACTGGTTTTGGAGGCAAATTCGGTGTTCAG
  TCGGAGAGGCAGGACTCCGCTGCTGTGGGGTTTGATTACAAGGAGAAGCTGGCCAAGCACGAGTCCCAG
  CAAGACTACTCCAAAGGATTCGGCGGGAAGTATGGGGTGCAGAAGGATCGGATGGATAAGAATGCGTCA
  ACCTTTGAGGATGTCACCCAGGTGTCCTCTGCCTACCAGAAGACAGTACCTGTCGAAGCTGTGACCAGC
  AAAACAAGTAACATCAGAGCTAACTTTGAAAACCTCGCTAAGGAGAAAGAGCAGGAGGACAGGCGGAAG
  GCGGAGGCGGAGAGAGCCCAGCGGATGGCCAAGGAGCGGCAGGAGCAGGAAGAGGCCAGGAGGAAGCTG
  GAGGAGCAAGCCAGAGCCAAAACGCAAACGCCCCCTGTGTCGCCCGCACCTCAGCCAACCGAGGAGAGG
  CTGCCCTCGAGCCCCGTCTATGAGGATGCGGCTTCCTTCAAGGCAGAGCTGAGCTACAGAGGCCCTGTG
  AGTGGGACGGAGCCGGAGCCCGTGTACAGCATGGAGGCCGCTGACTACCGAGAGGCCAGCAGCCAGCAG
  GGCCTGGCCTATGCCACAGAGGCTGTCTATGAAAGCGCAGAGGCCCCGGGCCACTATCCCGCAGAGGAC
  AGCACCTACGATGAGTACGAGAACGATCTGGGGATCACAGCCGTCGCCCTGTACGACTACCAGGCTGCG
  GGCGATGATGAGATCTCATTTGACCCTGATGACATCATCACCAACATCGAGATGATTGACGACGGCTGG
  TGGCGCGGGGTGTGCAAGGGCCGGTACGGGCTCTTCCCAGCCAACTATGTGGAGCTGCGGCAGTAG
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_005231
• Insert Size: 1653 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005231.3
• RefSeq Size: 3319 bp
• RefSeq ORF: 1653 bp
• Locus ID: 2017
• UniProt ID: Q14247
• Cytogenetics: 11q13.3
• Domains: SH3, HS1_rep
• Protein Families: Druggable Genome
• Protein Pathways: Pathogenic Escherichia coli infection, Tight junction
• MW: 61.6 kDa
• Gene Summary: This gene is overexpressed in breast cancer and squamous cell carcinomas of the head and neck. The encoded protein is localized in the cytoplasm and in areas of the cell-substratum contacts. This gene has two roles: (1) regulating the interactions between components of adherens-type junctions and (2) organizing the cytoskeleton and cell adhesion structures of epithelia and carcinoma cells. During apoptosis, the encoded protein is degraded in a caspase-dependent manner. The aberrant regulation of this gene contributes to tumor cell invasion and metastasis. Three splice variants that encode different isoforms have been identified for this gene. [provided by RefSeq, May 2010]
  Transcript Variant: This variant (1) is the longest transcript.