RRBP1 (NM_001042576) Human Untagged Clone

CAT#: SC311413

RRBP1 (untagged)-Human ribosome binding protein 1 homolog 180kDa (dog) (RRBP1), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RRBP1
• Synonyms: ES/130; ES130; hES; p180; RRp
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC311413 representing NM_001042576.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGATATTTACGACACTCAAACCTTGGGGGTTGTGGTCTTTGGAGGATTCATGGTTGTTTCTGCCATT
  GGCATCTTCCTGGTGTCGACTTTCTCCATGAAGGAAACGTCATATGAAGAAGCCCTAGCCAACCAGCGC
  AAGGAGATGGCGAAAACTCACCACCAGAAAGTCGAGAAGAAAAAGAAGGAGAAAACAGTGGAGAAGAAA
  GGAAAGACCAAGAAAAAGGAAGAGAAACCTAATGGGAAGATACCTGATCATGATCCAGCCCCCAATGTG
  ACTGTCCTCCTTCGAGAACCAGTGCGGGCTCCTGCTGTGGCTGTGGCTCCAACCCCAGTGCAGCCCCCC
  ATTATCGTTGCTCCTGTCGCCACAGTTCCAGCCATGCCCCAGGAGAAGCTGGCCTCCTCCCCCAAGGAC
  AAAAAGAAGAAGGAGAAAAAAGTGGCAAAAGTGGAACCAGCTGTCAGCTCTGTAGTGAATTCCATCCAG
  GTTCTCACTTCGAAGGCTGCCATCTTGGAAACTGCTCCCAAGGAGGGCAGAAATACAGATGTGGCCCAG
  AGCCCAGAGGCACCAAAGCAAGAGGCTCCTGCCAAGAAGAAGTCTGGTTCAAAGAAAAAAGGGCCCCCA
  GATGCCGACGGCCCTCTCTACCTCCCCTACAAGACGCTGGTCTCCACGGTTGGGAGCATGGTGTTCAAC
  GAGGGCGAGGCCCAGCGGCTCATCGAGATCCTGTCTGAGAAGGCTGGCATCATTCAGGACACCTGGCAC
  AAGGCCACTCAGAAGGGTGACCCTGTGGCGATTCTGAAACGCCAGCTGGAAGAGAAGGAAAAACTGCTG
  GCCACAGAACAGGAAGATGCGGCTGTCGCCAAGAGCAAACTGAGGGAGCTCAACAAGGAGATGGCAGCA
  GAAAAGGCCAAAGCAGCAGCCGGGGAGGCCAAAGTGAAAAAGCAGCTGGTGGCCCGGGAGCAGGAGATC
  ACGGCTGTGCAGGCACGCATGCAGGCCAGCTACCGGGAGCACGTGAAGGAGGTGCAGCAGCTGCAGGGC
  AAGATCCGGACTCTTCAGGAGCAGCTGGAGAATGGCCCCAACACGCAGCTGGCCCGCCTGCAGCAGGAG
  AACTCCATCCTGCGGGATGCCTTGAACCAGGCCACGAGCCAGGTGGAGAGCAAGCAGAACGCAGAGCTG
  GCCAAGCTTCGGCAGGAGCTCAGCAAGGTCAGCAAAGAGCTGGTGGAGAAGTCAGAGGCTGTGCGGCAA
  GATGAGCAGCAGCGGAAAGCTCTGGAAGCCAAGGCAGCTGCCTTCGAGAAGCAGGTCCTGCAGCTGCAG
  GCGTCCCACAGGGAGAGTGAGGAGGCCCTGCAGAAGCGCCTGGACGAGGTCAGCCGGGAGCTGTGCCAC
  ACGCAGAGCAGCCACGCCAGCCTCCGGGCGGATGCCGAGAAGGCCCAGGAGCAACAGCAGCAGATGGCC
  GAGCTGCACAGCAAGTTACAGTCCTCCGAGGCGGAGGTGCGCAGCAAATGCGAGGAGCTGAGTGGCCTC
  CACGGGCAGCTCCAGGAGGCCAGGGCGGAGAACTCCCAGCTCACAGAGAGAATCCGTTCCATTGAGGCC
  CTGCTGGAGGCGGGCCAGGCGCGGGATGCCCAGGACGTCCAGGCCAGCCAGGCGGAGGCTGACCAGCAG
  CAGACTCGCCTCAAGGAGCTGGAGTCCCAGGTGTCGGGTCTGGAGAAGGAGGCCATCGAGCTCAGGGAG
  GCCGTCGAGCAGCAGAAAGTGAAGAACAATGACCTCCGGGAGAAGAACTGGAAGGCCATGGAGGCACTG
  GCCACGGCCGAGCAGGCCTGCAAGGAGAAGCTGCACTCCCTGACCCAGGCCAAGGAGGAATCGGAGAAG
  CAGCTCTGTCTGATTGAGGCGCAGACCATGGAGGCCCTGCTGGCTCTGCTCCCAGAACTCTCTGTCTTG
  GCACAACAGAATTACACCGAGTGGCTGCAGGATCTCAAAGAGAAAGGCCCCACGCTGCTGAAGCACCCG
  CCAGCTCCCGCGGAGCCCTCCTCGGACCTGGCCTCCAAGTTGAGGGAGGCCGAGGAGACGCAGAGCACA
  CTGCAGGCCGAGTGTGACCAGTACCGCAGCATCCTGGCGGAGACGGAGGGCATGCTCAGAGACCTGCAG
  AAGAGCGTGGAGGAGGAGGAGCAGGTGTGGAGGGCCAAGGTGGGCGCCGCAGAGGAGGAGCTCCAGAAG
  TCCCGGGTCACAGTGAAGCATCTCGAAGAGATTGTAGAGAAGCTAAAAGGAGAACTTGAAAGTTCGGAC
  CAGGTGAGGGAGCACACGTCGCATTTGGAGGCAGAGCTGGAAAAGCACATGGCGGCCGCCAGCGCCGAG
  TGCCAGAACTACGCCAAGGAGGTGGCAGGGCTGAGGCAACTTCTCCTAGAATCTCAATCTCAGCTCGAT
  GCCGCCAAGAGCGAAGCCCAGAAACAGAGCGATGAGCTTGCCCTGGTCAGGCAGCAGTTGAGTGAAATG
  AAGAGCCACGTAGAGGATGGTGACATAGCTGGGGCCCCAGCTTCCTCCCCAGAGGCGCCCCCAGCCGAG
  CAGGACCCCGTTCAGCTGAAGACGCAGCTGGAGTGGACAGAAGCCATCCTGGAGGATGAGCAGACACAG
  CGGCAGAAGCTCACGGCCGAGTTTGAGGAGGCTCAGACCTCGGCATGTCGGTTACAAGAAGAATTGGAG
  AAGCTCCGCACAGCCGGCCCCCTAGAGTCTTCAGAAACAGAGGAGGCCTCACAGCTGAAGGAGAGACTA
  GAAAAAGAGAAGAAGTTAACAAGTGACCTGGGGCGCGCCGCCACGAGACTGCAGGAGCTTCTGAAGACG
  ACCCAGGAGCAGCTGGCAAGGGAGAAGGACACGGTGAAGAAGCTGCAGGAACAGCTGGAAAAGGCAGAG
  GACGGCAGCAGCTCAAAGGAGGGCACCTCTGTCTGA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001042576
• Insert Size: 2934 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001042576.1
• RefSeq Size: 3792 bp
• RefSeq ORF: 2934 bp
• Locus ID: 6238
• UniProt ID: Q9P2E9
• Cytogenetics: 20p12.1
• Protein Families: Druggable Genome, Transmembrane
• MW: 108.7 kDa
• Gene Summary: This gene encodes a ribosome-binding protein of the endoplasmic reticulum (ER) membrane. Studies suggest that this gene plays a role in ER proliferation, secretory pathways and secretory cell differentiation, and mediation of ER-microtubule interactions. Alternative splicing has been observed and protein isoforms are characterized by regions of N-terminal decapeptide and C-terminal heptad repeats. Splicing of the tandem repeats results in variations in ribosome-binding affinity and secretory function. The full-length nature of variants which differ in repeat length has not been determined. Pseudogenes of this gene have been identified on chromosomes 3 and 7, and RRBP1 has been excluded as a candidate gene in the cause of Alagille syndrome, the result of a mutation in a nearby gene on chromosome 20p12. [provided by RefSeq, Apr 2012]
  Transcript Variant: This variant (1) represents the longer variant.