CA150 (TCERG1) (NM_001040006) Human Untagged Clone

CAT#: SC311063

TCERG1 (untagged)-Human transcription elongation regulator 1 (TCERG1), transcript variant 2

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TCERG1
• Synonyms: CA150; TAF2S; Urn1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC311063 representing NM_001040006.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGCGGAGCGTGGCGGGGACGGGGGCGAGAGTGAACGATTCAACCCGGGGGAGCTCAGGATGGCCCAA
  CAGCAGGCCTTGAGGTTCCGAGGTCCGGCTCCCCCACCAAATGCAGTGATGCGAGGCCCACCACCTCTG
  ATGCGACCTCCTCCACCTTTTGGTATGATGCGAGGCCCTCCTCCACCACCACGGCCGCCCTTTGGACGT
  CCTCCTTTTGATCCTAATATGCCGCCAATGCCTCCTCCAGGAGGGATACCTCCACCTATGGGCCCTCCA
  CACCTCCAGAGACCACCTTTCATGCCTCCTCCCATGAGTTCCATGCCTCCTCCTCCGGGTATGATGTTT
  CCACCAGGAATGCCTCCTGTGACTGCTCCTGGTACTCCAGCACTACCTCCTACGGAGGAGATATGGGTT
  GAAAATAAAACTCCAGATGGGAAGGTTTATTATTATAATGCTCGGACACGTGAATCTGCATGGACCAAG
  CCAGATGGAGTTAAGGTTATTCAGCAATCAGAACTGACACCTATGCTTGCAGCCCAGGCACAGGTTCAG
  GCTCAGGCCCAGGCGCAGGCTCAGGCCCAGGCGCAGGCTCAGGCCCAGGCACAAGCTCAGGCCCAGGCT
  CAGGCTCAGGCCCAGGCCCAGGCCCAGGCCCAGGCCCAGGCCCAAGCCCAAGCCCAGGCCCAGGCTCAG
  GCTCAGGCACAAGCTCAGGCCCAGGCCCAGGCTCAGGTCCAGGCCCAGGTCCAGGCACAAGTGCAAGCA
  CAAGCAGTTGGAGCTTCCACCCCTACGACCAGTAGCCCAGCACCTGCAGTATCCACTTCAACATCATCA
  TCCACCCCTTCCTCTACCACTTCTACCACAACAACTGCTACTTCAGTTGCGCAGACAGTATCAACACCC
  ACAACACAAGATCAGACCCCAAGTTCTGCTGTTTCAGTTGCCACGCCTACAGTTAGTGTTTCAACTCCT
  GCTCCTACAGCCACACCTGTGCAAACCGTTCCCCAGCCGCACCCTCAGACGTTACCTCCTGCTGTTCCT
  CATTCAGTACCTCAGCCAACAACAGCAATACCTGCTTTTCCACCAGTAATGGTACCTCCGTTTCGTGTT
  CCCCTTCCTGGCATGCCAATTCCACTTCCAGGTGTATTGCCAGGAATGGCCCCTCCTATCGTACCCATG
  ATACATCCCCAGGTTGCTATTGCAGCTTCACCTGCTACCTTAGCTGGAGCAACAGCAGTTTCTGAATGG
  ACTGAATATAAAACAGCAGATGGGAAGACATATTATTATAATAATAGAACATTAGAATCAACCTGGGAA
  AAACCCCAAGAACTAAAGGAAAAAGAAAAGTTAGAAGAGAAGATTAAAGAGCCAATTAAAGAACCCTCT
  GAAGAGCCTCTGCCAATGGAGACGGAGGAGGAGGATCCTAAAGAAGAGCCTATAAAGGAGATAAAGGAG
  GAGCCCAAAGAAGAGGAGATGACTGAAGAAGAAAAGGCTGCCCAGAAGGCAAAGCCAGTTGCTACTGCT
  CCTATTCCTGGTACTCCATGGTGTGTCGTTTGGACTGGTGATGAGCGGGTCTTCTTTTATAATCCCACC
  ACTCGTCTTTCTATGTGGGACCGACCTGATGATCTGATTGGCAGGGCAGATGTTGACAAAATTATTCAG
  GAGCCCCCTCATAAAAAAGGAATGGAGGAATTGAAGAAACTAAGGCACCCAACTCCGACAATGCTGTCG
  ATCCAAAAGTGGCAATTCTCTATGAGTGCAATTAAAGAGGAACAAGAATTAATGGAAGAAATTAATGAA
  GATGAGCCTGTTAAAGCAAAAAAACGGAAGAGAGACGATAATAAAGACATTGACTCAGAGAAAGAAGCT
  GCCATGGAAGCTGAAATTAAAGCTGCCCGAGAAAGGGCCATTGTCCCTCTGGAGGCTCGAATGAAGCAG
  TTCAAGGACATGCTGCTAGAGAGAGGGGTGTCTGCTTTTTCAACGTGGGAGAAGGAGTTGCACAAGATA
  GTTTTTGATCCCCGGTACTTACTTCTCAATCCTAAAGAGAGAAAACAGGTGTTTGATCAGTATGTAAAG
  ACCAGGGCAGAGGAAGAACGCAGGGAAAAGAAAAATAAAATAATGCAAGCCAAGGAAGATTTCAAAAAA
  ATGATGGAAGAAGCAAAATTTAATCCAAGAGCAACTTTTAGTGAATTTGCAGCCAAGCATGCTAAAGAT
  TCAAGATTCAAAGCAATTGAAAAGATGAAAGACCGAGAAGCCTTGTTTAATGAGTTTGTGGCCGCTGCT
  AGGAAGAAAGAGAAAGAAGATTCGAAGACCAGAGGTGAGAAGATTAAATCGGATTTCTTTGAACTATTA
  TCTAATCATCACTTGGACAGTCAGTCTCGATGGAGCAAAGTAAAAGACAAAGTAGAAAGTGATCCACGT
  TACAAAGCAGTAGATAGTTCATCAATGAGAGAAGACCTTTTCAAACAGTACATTGAAAAAATAGCCAAG
  AATTTAGACTCAGAAAAAGAAAAGGAGCTTGAAAGGCAAGCCCGCATTGAGGCAAGCCTTCGAGAACGA
  GAAAGGGAGGTTCAAAAGGCCCGTTCAGAACAAACAAAAGAAATAGATCGAGAGAGAGAGCAGCACAAA
  CGAGAAGAAGCTATCCAGAATTTCAAAGCTCTTCTGTCTGACATGGTACGTTCTTCAGATGTGTCATGG
  TCTGATACTCGTAGGACCCTCCGAAAAGATCACCGCTGGGAATCTGGATCCTTATTGGAAAGAGAGGAG
  AAAGAGAAGCTTTTTAATGAACACATTGAAGCACTTACCAAAAAAAAGAGAGAGCACTTTAGGCAACTT
  CTGGATGAAACTTCTGCAATTACCTTAACATCCACGTGGAAAGAAGTAAAAAAAATCATTAAGGAAGAT
  CCTCGATGTATTAAGTTCTCCTCCAGTGACAGGAAAAAACAAAGAGAATTTGAAGAATATATCAGAGAC
  AAATATATCACAGCCAAAGCTGACTTCAGGACGCTTTTGAAAGAGACCAAATTTATAACATATAGATCC
  AAAAAATTAATCCAAGAATCAGATCAGCACCTGAAAGATGTAGAAAAAATTTTACAGAATGACAAACGG
  TATCTAGTACTGGACTGTGTGCCAGAGGAGAGGCGTAAACTGATTGTGGCATATGTTGATGACCTGGAT
  CGCCGGGGTCCACCCCCACCTCCCACAGCATCGGAGCCCACGAGACGATCAACAAAATAA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_001040006
• Insert Size: 3234 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001040006.1
• RefSeq Size: 4170 bp
• RefSeq ORF: 3234 bp
• Locus ID: 10915
• UniProt ID: O14776
• Cytogenetics: 5q32
• Protein Families: Stem cell - Pluripotency, Transcription Factors
• Protein Pathways: Spliceosome
• MW: 121.7 kDa
• Gene Summary: This gene encodes a nuclear protein that regulates transcriptional elongation and pre-mRNA splicing. The encoded protein interacts with the hyperphosphorylated C-terminal domain of RNA polymerase II via multiple FF domains, and with the pre-mRNA splicing factor SF1 via a WW domain. Alternative splicing results in multiple transcripts variants encoding different isoforms. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (2) lacks an alternate in-frame exon compared to variant 1, resulting in a shorter protein (isoform 2) compared to isoform 1.