ERCC8 (NM_000082) Human Untagged Clone

CAT#: SC310441

ERCC8 (untagged)-Human excision repair cross-complementing rodent repair deficiency, complementation group 8 (ERCC8), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: ERCC8
• Synonyms: CKN1; CSA; UVSS2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC310441 representing NM_000082.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGCTGGGGTTTTTGTCCGCACGCCAAACGGGTTTGGAGGACCCTCTTCGCCTTCGGAGAGCAGAGTCA
  ACACGGAGAGTTTTGGGACTGGAATTAAATAAAGACAGAGATGTTGAAAGAATCCACGGCGGTGGAATT
  AACACCCTTGACATTGAACCTGTTGAAGGGAGATACATGTTATCAGGTGGTTCAGATGGTGTGATTGTA
  CTTTATGACCTTGAGAACTCCAGCAGACAATCTTATTACACATGTAAAGCAGTGTGTTCCATTGGCAGA
  GATCATCCTGATGTTCACAGATACAGTGTGGAGACTGTACAGTGGTATCCTCATGACACTGGCATGTTC
  ACATCAAGCTCATTTGATAAAACTCTGAAAGTATGGGATACAAATACATTACAAACTGCAGATGTATTT
  AATTTTGAGGAAACAGTTTATAGTCATCATATGTCTCCAGTCTCCACCAAGCACTGTTTGGTAGCAGTT
  GGTACTAGAGGACCCAAAGTACAACTTTGTGACTTGAAGTCTGGATCCTGTTCTCACATTCTACAGGGT
  CACAGACAAGAAATATTAGCAGTTTCCTGGTCTCCACGTTATGACTATATCTTGGCAACAGCAAGTGCT
  GACAGTAGAGTAAAATTATGGGATGTGAGAAGAGCATCAGGATGTTTGATTACTCTTGATCAACATAAT
  GGGAAAAAGTCACAAGCTGTTGAATCAGCAAACACTGCTCATAATGGGAAAGTTAATGGCTTATGTTTT
  ACAAGTGATGGACTTCACCTCCTCACTGTTGGTACAGATAATCGAATGAGGCTCTGGAATAGTTCCAAT
  GGAGAAAACACACTTGTGAACTATGGAAAAGTTTGTAATAACAGTAAAAAAGGATTGAAATTCACTGTC
  TCCTGTGGCTGCAGTTCAGAATTTGTTTTTGTACCATATGGTAGCACCATTGCTGTTTATACAGTTTAC
  TCAGGAGAACAGATAACTATGCTTAAGGGACATTATAAAACTGTTGACTGCTGTGTATTTCAGTCAAAT
  TTCCAGGAACTTTATAGTGGTAGCAGAGACTGCAACATTCTGGCTTGGGTTCCATCCTTATATGAACCA
  GTTCCTGATGATGATGAGACTACAACAAAATCACAATTAAATCCGGCCTTTGAAGATGCCTGGAGCAGC
  AGTGATGAAGAAGGATGA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_000082
• Insert Size: 1191 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000082.3
• RefSeq Size: 2044 bp
• RefSeq ORF: 1191 bp
• Locus ID: 1161
• UniProt ID: Q13216
• Cytogenetics: 5q12.1
• Domains: WD40
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Nucleotide excision repair, Ubiquitin mediated proteolysis
• MW: 44.1 kDa
• Gene Summary: This gene encodes a WD repeat protein, which interacts with Cockayne syndrome type B (CSB) protein and with p44 protein, a subunit of the RNA polymerase II transcription factor IIH. Mutations in this gene have been identified in patients with hereditary disease Cockayne syndrome (CS). CS cells are abnormally sensitive to ultraviolet radiation and are defective in the repair of transcriptionally active genes. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2014]
  Transcript Variant: This variant (1) encodes the longest isoform (1).