ERCC8 (NM_000082) Human Tagged ORF Clone

CAT#: RG214392

ERCC8 (tGFP-tagged) - Human excision repair cross-complementing rodent repair deficiency, complementation group 8 (ERCC8), transcript variant 1

Product Images

Circular map for RG214392

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: ERCC8
• Synonyms: CKN1; CSA; UVSS2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG214392 representing NM_000082
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGGGGTTTTTGTCCGCACGCCAAACGGGTTTGGAGGACCCTCTTCGCCTTCGGAGAGCAGAGTCAA
  CACGGAGAGTTTTGGGACTGGAATTAAATAAAGACAGAGATGTTGAAAGAATCCACGGCGGTGGAATTAA
  CACCCTTGACATTGAACCTGTTGAAGGGAGATACATGTTATCAGGTGGTTCAGATGGTGTGATTGTACTT
  TATGACCTTGAGAACTCCAGCAGACAATCTTATTACACATGTAAAGCAGTGTGTTCCATTGGCAGAGATC
  ATCCTGATGTTCACAGATACAGTGTGGAGACTGTACAGTGGTATCCTCATGACACTGGCATGTTCACATC
  AAGCTCATTTGATAAAACTCTGAAAGTATGGGATACAAATACATTACAAACTGCAGATGTATTTAATTTT
  GAGGAAACAGTTTATAGTCATCATATGTCTCCAGTCTCCACCAAGCACTGTTTGGTAGCAGTTGGTACTA
  GAGGACCCAAAGTACAACTTTGTGACTTGAAGTCTGGATCCTGTTCTCACATTCTACAGGGTCACAGACA
  AGAAATATTAGCAGTTTCCTGGTCTCCACGTTATGACTATATCTTGGCAACAGCAAGTGCTGACAGTAGA
  GTAAAATTATGGGATGTGAGAAGAGCATCAGGATGTTTGATTACTCTTGATCAACATAATGGGAAAAAGT
  CACAAGCTGTTGAATCAGCAAACACTGCTCATAATGGGAAAGTTAATGGCTTATGTTTTACAAGTGATGG
  ACTTCACCTCCTCACTGTTGGTACAGATAATCGAATGAGGCTCTGGAATAGTTCCAATGGAGAAAACACA
  CTTGTGAACTATGGAAAAGTTTGTAATAACAGTAAAAAAGGATTGAAATTCACTGTCTCCTGTGGCTGCA
  GTTCAGAATTTGTTTTTGTACCATATGGTAGCACCATTGCTGTTTATACAGTTTACTCAGGAGAACAGAT
  AACTATGCTTAAGGGACATTATAAAACTGTTGACTGCTGTGTATTTCAGTCAAATTTCCAGGAACTTTAT
  AGTGGTAGCAGAGACTGCAACATTCTGGCTTGGGTTCCATCCTTATATGAACCAGTTCCTGATGATGATG
  AGACTACAACAAAATCACAATTAAATCCGGCCTTTGAAGATGCCTGGAGCAGCAGTGATGAAGAAGGA
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG214392 representing NM_000082
  Red=Cloning site Green=Tags(s)
  
  MLGFLSARQTGLEDPLRLRRAESTRRVLGLELNKDRDVERIHGGGINTLDIEPVEGRYMLSGGSDGVIVL
  YDLENSSRQSYYTCKAVCSIGRDHPDVHRYSVETVQWYPHDTGMFTSSSFDKTLKVWDTNTLQTADVFNF
  EETVYSHHMSPVSTKHCLVAVGTRGPKVQLCDLKSGSCSHILQGHRQEILAVSWSPRYDYILATASADSR
  VKLWDVRRASGCLITLDQHNGKKSQAVESANTAHNGKVNGLCFTSDGLHLLTVGTDNRMRLWNSSNGENT
  LVNYGKVCNNSKKGLKFTVSCGCSSEFVFVPYGSTIAVYTVYSGEQITMLKGHYKTVDCCVFQSNFQELY
  SGSRDCNILAWVPSLYEPVPDDDETTTKSQLNPAFEDAWSSSDEEG
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000082
• ORF Size: 1188 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000082.4
• RefSeq Size: 2031 bp
• RefSeq ORF: 1191 bp
• Locus ID: 1161
• UniProt ID: Q13216
• Cytogenetics: 5q12.1
• Domains: WD40
• Protein Families: Druggable Genome, Transcription Factors
• Protein Pathways: Nucleotide excision repair, Ubiquitin mediated proteolysis
• Gene Summary: This gene encodes a WD repeat protein, which interacts with Cockayne syndrome type B (CSB) protein and with p44 protein, a subunit of the RNA polymerase II transcription factor IIH. Mutations in this gene have been identified in patients with hereditary disease Cockayne syndrome (CS). CS cells are abnormally sensitive to ultraviolet radiation and are defective in the repair of transcriptionally active genes. Several transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Mar 2014]