TRIM16 (NM_006470) Human Untagged Clone

CAT#: SC310262

TRIM16 (untagged)-Human tripartite motif containing 16 (TRIM16)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TRIM16
• Synonyms: EBBP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC310262 representing NM_006470.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGCTGAGTTGGATCTAATGGCTCCAGGGCCACTGCCCAGGGCCACTGCTCAGCCCCCAGCCCCTCTC
  AGCCCAGACTCTGGGTCACCCAGCCCAGATTCTGGGTCAGCCAGCCCAGTGGAAGAAGAGGACGTGGGC
  TCCTCGGAGAAGCTTGGCAGGGAGACGGAGGAACAGGACAGCGACTCTGCAGAGCAGGGGGATCCTGCT
  GGTGAGGGGAAAGAGGTCCTGTGTGACTTCTGCCTTGATGACACCAGAAGAGTGAAGGCAGTGAAGTCC
  TGTCTAACCTGCATGGTGAATTACTGTGAAGAGCACTTGCAGCCGCATCAGGTGAACATCAAACTGCAA
  AGCCACCTGCTGACCGAGCCAGTGAAGGACCACAACTGGCGATACTGCCCTGCCCACCACAGCCCACTG
  TCTGCCTTCTGCTGCCCTGATCAGCAGTGCATCTGCCAGGACTGTTGCCAGGAGCACAGTGGCCACACC
  ATAGTCTCCCTGGATGCAGCCCGCAGGGACAAGGAGGCTGAACTCCAGTGCACCCAGTTAGACTTGGAG
  CGGAAACTCAAGTTGAATGAAAATGCCATCTCCAGGCTCCAGGCTAACCAAAAGTCTGTTCTGGTGTCG
  GTGTCAGAGGTCAAAGCGGTGGCTGAAATGCAGTTTGGGGAACTCCTTGCTGCTGTGAGGAAGGCCCAG
  GCCAATGTGATGCTCTTCTTAGAGGAGAAGGAGCAAGCTGCGCTGAGCCAGGCCAACGGTATCAAGGCC
  CACCTGGAGTACAGGAGTGCCGAGATGGAGAAGAGCAAGCAGGAGCTGGAGAGGATGGCGGCCATCAGC
  AACACTGTCCAGTTCTTGGAGGAGTACTGCAAGTTTAAGAACACTGAAGACATCACCTTCCCTAGTGTT
  TACGTAGGGCTGAAGGATAAACTCTCGGGCATCCGCAAAGTTATCACGGAATCCACTGTACACTTAATC
  CAGTTGCTGGAGAACTATAAGAAAAAGCTCCAGGAGTTTTCCAAGGAAGAGGAGTATGACATCAGAACT
  CAAGTGTCTGCCGTTGTTCAGCGCAAATATTGGACTTCCAAACCTGAGCCCAGCACCAGGGAACAGTTC
  CTCCAATATGCGTATGACATCACGTTTGACCCGGACACAGCACACAAGTATCTCCGGCTGCAGGAGGAG
  AACCGCAAGGTCACCAACACCACGCCCTGGGAGCATCCCTACCCGGACCTCCCCAGCAGGTTCCTGCAC
  TGGCGGCAGGTGCTGTCCCAGCAGAGTCTGTACCTGCACAGGTACTATTTTGAGGTGGAGATCTTCGGG
  GCAGGCACCTATGTTGGCCTGACCTGCAAAGGCATCGACCGGAAAGGGGAGGAGCGCAACAGTTGCATT
  TCCGGAAACAACTTCTCCTGGAGCCTCCAATGGAACGGGAAGGAGTTCACGGCCTGGTACAGTGACATG
  GAGACCCCACTCAAAGCTGGCCCTTTCCGGAGGCTCGGGGTCTATATCGACTTCCCGGGAGGGATCCTT
  TCCTTCTATGGCGTAGAGTATGATACCATGACTCTGGTTCACAAGTTTGCCTGCAAATTTTCAGAACCA
  GTCTATGCTGCCTTCTGGCTTTCCAAGAAGGAAAACGCCATCCGGATTGTAGATCTGGGAGAGGAACCC
  GAGAAGCCAGCACCGTCCTTGGTGGGGACTGCTCCCTAG
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_006470
• Insert Size: 1695 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006470.3
• RefSeq Size: 2920 bp
• RefSeq ORF: 1695 bp
• Locus ID: 10626
• UniProt ID: O95361
• Cytogenetics: 17p12
• Domains: zf-B_box, SPRY, PRY
• Protein Families: Druggable Genome, Transcription Factors
• MW: 64 kDa
• Gene Summary: The protein encoded by this gene is a tripartite motif (TRIM) family member that contains two B box domains and a coiled-coiled region that are characteristic of the B box zinc finger protein family. While it lacks a RING domain found in other TRIM proteins, the encoded protein can homodimerize or heterodimerize with other TRIM proteins and has E3 ubiquitin ligase activity. This gene is also a tumor suppressor and is involved in secretory autophagy. [provided by RefSeq, Jan 2017]
  Transcript Variant: This variant (3), as well as variants 1 and 2, encodes isoform a.