TIAM1 (NM_003253) Human Untagged Clone

CAT#: SC309903

TIAM1 (untagged)-Human T-cell lymphoma invasion and metastasis 1 (TIAM1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: TIAM1
• Synonyms: TIAM-1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_003253 edited
  ATGGGAAACGCAGAAAGTCAACATGTAGAGCACGAGTTTTATGGAGAAAAGCATGCCAGC
  CTGGGGCGCAAGCACACTTCCCGCTCCCTGCGCCTCTCGCACAAGACGCGGAGGACCAGG
  CACGCTTCCTCGGGGAAGGTGATCCACAGGAACTCCGAAGTGAGCACCCGATCCAGCAGC
  ACCCCCAGCATCCCCCAGTCCCTGGCTGAAAATGGCCTGGAGCCCTTCTCCCAAGATGGT
  ACCCTAGAAGACTTCGGGAGCCCCATCTGGGTGGACCGAGTGGACATGGGCTTGAGACCT
  GTGTCTTACACTGACTCTTCTGTCACTCCCAGCGTAGACAGCAGCATCGTCCTCACAGCA
  GCCTCTGTGCAGAGCATGCCAGACACTGAGGAGAGCAGGCTTTACGGGGATGACGCTACA
  TATTTGGCTGAGGGAGGCAGGAGGCAGCATTCCTATACATCCAATGGGCCCACTTTCATG
  GAGACGGCGAGCTTTAAGAAGAAACGCTCCAAATCTGCAGACATCTGGCGGGAGGACAGC
  CTGGAATTCTCACTCTCTGATCTGAGCCAAGAACATTTAACAAGCAACGAAGAAATCTTG
  GGTTCCGCCGAAGAGAAGGACTGCGAGGAGGCTCGGGGGATGGAAACGCGGGCGAGTCCG
  CGGCAGCTCAGCACCTGTCAGAGAGCCAATTCCTTGGGTGACTTGTATGCTCAGAAAAAC
  TCTGGAGTGACAGCAAACGGGGGGCCGGGGAGCAAATTTGCAGGCTACTGTCGGAATTTG
  GTGTCTGATATTCCCAATCTTGCAAACCATAAGATGCCACCAGCTGCTGCTGAAGAGACT
  CCTCCGTACAGTAATTATAACACACTTCCCTGTAGGAAATCTCACTGTCTCTCTGAAGGT
  GCCACCAACCCACAAATTAGCCATAGCAACAGCATGCAAGGCAGAAGAGCTAAAACAACT
  CAGGATGTTAATGCAGGCGAGGGCAGTGAGTTTGCAGACAGTGGGATTGAAGGGGCCACT
  ACCGACACGGACCTCCTGTCCAGGCGATCTAATGCCACCAACTCCAGCTACTCACCCACC
  ACAGGCCGGGCCTTTGTGGGCAGCGACAGCGGCAGCAGCTCCACCGGGGATGCGGCTCGT
  CAGGGGGTGTACGAGAACTTCCGGCGGGAGCTGGAGATGAGCACCACCAACAGCGAGAGC
  CTGGAGGAGGCCGGCTCGGCGCACAGCGATGAGCAGAGCAGCGGCACCCTGAGCTCTCCG
  GGCCAGTCGGACATCCTGCTGACCGCCGCACAGGGCACGGTGCGCAAGGCCGGCGCCCTG
  GCCGTCAAGAACTTCCTGGTGCACAAGAAGAACAAGAAGGTGGAGTCAGCCACCCGGAGG
  AAGTGGAAGCACTACTGGGTGTCCCTGAAAGGATGCACGCTATTTTTCTACGAGAGCGAC
  GGCAGGTCTGGGATAGACCACAACAGCATCCCCAAACACGCCGTCTGGGTGGAGAACAGC
  ATTGTGCAGGCGGTGCCTGAGCACCCCAAGAAGGACTTTGTCTTCTGCCTCAGCAATTCC
  CTGGGTGATGCCTTCCTTTTTCAGACCACTAGCCAGACGGAGCTTGAAAACTGGATCACC
  GCCATCCACTCTGCCTGCGCCACTGCGGTCGCGAGGCACCACCACAAGGAAGACACGCTC
  CGACTCCTGAAATCAGAGATCAAAAAACTGGAACAGAAGATTGACATGGATGAAAAGATG
  AAGAAAATGGGTGAAATGCAGCTGTCTTCAGTCACTGACTCAAAGAAAAAGAAAACAATA
  TTAGATCAGATCTTTGTCTGGGAGCAAAATCTCGAGCAGTTCCAAATGGACCTGTTTCGT
  TTCCGCTGTTATTTAGCCAGCCTTCAGGGTGGGGAGCTGCCAAACCCCAAAAGGCTTCTC
  GCTTTTGCAAGTCGACCAACGAAAGTGGCCATGGGCCGCCTTGGAATCTTTTCGGTATCA
  TCGTTTCATGCCCTGGTGGCAGCACGCACTGGTGAAACTGGAGTGAGAAGACGTACTCAG
  GCCATGTCCAGATCCGCGAGCAAGCGAAGGAGCAGGTTTTCTTCTCTGTGGGGTCTGGAT
  ACTACCTCCAAAAAGAAGCAGGGACGGCCAAGCATCAATCAGGTGTTTGGAGAGGGAACC
  GAAGCTGTAAAGAAATCTTTAGAGGGAATATTTGATGACATTGTTCCAGATGGCAAGAGG
  GAGAAAGAAGTGGTCTTACCTAACGTTCACCAGCACAACCCTGACTGCGACATTTGGGTC
  CACGAGTATTTCACTCCATCCTGGTTCTGTCTGCCCAATAATCAGCCTGCCCTGACGGTC
  GTCCGGCCAGGCGACACTGCACGGGACACCCTGGAGCTGATTTGCAAGACACATCAACTG
  GATCATTCTGCTCATTACCTGCGCCTGAAATTTCTAATAGAAAACAAAATGCAGCTCTAT
  GTTCCACAGCCCGAGGAAGACATCTATGAGCTGCTGTACAAAGAAATTGAAATCTGTCCA
  AAAGTCACTCAGAGCATCCACATTGAGAAGTCAGATACAGCTGCTGATACTTACGGGTTT
  TCACTTTCTTCTGTGGAAGAAGATGGTATTCGAAGGCTGTACGTGAATAGTGTGAAGGAA
  ACCGGTTTAGCTTCCAAGAAAGGCCTGAAAGCAGGAGATGAGATTCTTGAGATCAATAAT
  CGTGCTGCTGACGCCCTGAACTCTTCTATGCTCAAAGATTTCCTCTCACAGCCCTCGCTG
  GGCCTCCTGGTGAGGACCTACCCCGAGCTGGAGGAAGGAGTGGAGCTGCTGGAAAGCCCG
  CCCCACCGAGTGGACGGCCCTGCCGACCTTGGCGAGAGCCCCCTCGCCTTTCTCACCAGC
  AACCCAGGGCACAGCCTTTGCAGCGAGCAGGGCAGCAGTGCTGAGACCGCTCCAGAGGAG
  ACCGAGGGGCCAGACTTGGAATCCTCAGATGAGACTGATCACAGCAGCAAGAGTACAGAA
  CAGGTGGCCGCATTTTGCCGCAGTTTGCATGAGATGAACCCCTCTGACCAGAGCCCATCT
  CCTCAGGACTCCACGGGGCCTCAGCTGGCGACCATGAGACAACTCTCGGATGCAGATAAG
  CTGCGCAAGGTGATCTGCGAGCTCCTGGAGACGGAGCGCACCTACGTGAAGGATTTAAAC
  TGTCTTATGGAGAGATACCTAAAGCCTCTTCAAAAAGAAACTTTTCTCACCCAGGATGAG
  CTTGACGTGCTTTTTGGAAATTTAACGGAAATGGTAGAGTTTCAAGTAGAATTCCTTAAA
  ACTCTAGAAGATGGAGTGAGACTGGTACCTGATTTGGAAAAGCTTGAGAAGGTTGATCAA
  TTTAAGAAAGTGCTGTTCTCTCTGGGGGGATCATTCCTGTATTATGCTGACCGCTTCAAG
  CTCTACAGTGCCTTCTGCGCCAGCCACACAAAAGTTCCCAAGGTCCTGGTGAAAGCCAAG
  ACAGACACGGCTTTCAAGGCATTCTTGGATGCCCAGAACCCGAAGCAGCAGCACTCATCC
  ACGCTGGAGTCGTACCTCATCAAGCCCATCCAGAGGATCCTCAAGTACCCACTTCTGCTC
  AGGGAGCTGTTCGCCCTGACCGATGCGGAGAGCGAGGAGCACTACCACCTGGACGTGGCC
  ATCAAGACCATGAACAAGGTTGCCAGTCACATCAATGAGATGCAGAAAATCCATGAAGAG
  TTTGGGGCTGTGTTTGACCAGCTGATTGCTGAACAGACTGGTGAGAAAAAAGAGGTTGCA
  GATCTGAGCATGGGAGACCTGCTTTTGCACACTACCGTGATCTGGCTGAACCCGCCGGCC
  TCGCTGGGCAAGTGGAAAAAGGAACCAGAGTTGGCAGCATTCGTCTTCAAAACTGCTGTG
  GTCCTTGTGTATAAAGATGGTTCCAAACAGAAGAAGAAACTTGTAGGATCTCACAGGCTT
  TCCATTTATGAGGACTGGGACCCCTTCAGATTTCGACACATGATCCCCACGGAAGCGCTG
  CAGGTTCGAGCTTTGGCGAGTGCAGATGCAGAGGCAAATGCCGTGTGTGAAATTGTCCAT
  GTAAAATCCGAGTCTGAAGGGAGGCCGGAGAGGGTCTTTCACTTGTGCTGCAGCTCCCCA
  GAGAGCCGAAAGGATTTCCTAAAGGCTGTGCATTCAATCCTGCGTGATAAGCACAGAAGA
  CAGCTCCTCAAAACCGAGAGCCTTCCCTCATCCCAGCAATATGTCCCTTTTGGAGGCAAA
  AGATTGTGTGCACTGAAGGGGGCCAGGCCGGCCATGAGCAGGGCAGTGTCTGCCCCAAGC
  AAGTCTCTTGGGAGGAGGAGGCGGCGGCTGGCTCGAAACAGGTTTACCATTGATTCTGAT
  GCCGTCTCCGCAAGCAGCCCGGAGAAAGAGTCCCAGCAGCCCCCCGGTGGTGGGGACACT
  GACCGATGGGTAGAGGAGCAGTTTGATCTTGCTCAGTATGAGGAGCAAGATGACATCAAG
  GAGACAGACATCCTCAGTGACGATGATGAGTTCTGTGAGTCCGTGAAGGGTGCCTCAGTG
  GACAGAGACCTGCAGGAGCGGCTTCAGGCCACCTCCATCAGTCAGCGGGAAAGAGGCCGG
  AAAACCCTGGATAGTCACGCGTCCCGCATGGCACAGCTCAAGAAGCAAGCTGCCCTGTCG
  GGGATCAATGGAGGCCTGGAGAGCGCAAGCGAGGAAGTCATTTGGGTTAGGCGTGAAGAC
  TTTGCCCCCTCCAGGAAACTGAACACTGAGATCTGA
  
• Restriction Sites: Please inquire
• ACCN: NM_003253
• Insert Size: 4800 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003253.1, NP_003244.1
• RefSeq Size: 5521 bp
• RefSeq ORF: 4776 bp
• Locus ID: 7074
• UniProt ID: Q13009
• Cytogenetics: 21q22.11
• Domains: RhoGEF, PDZ, PH, RBD
• Protein Families: Druggable Genome
• Protein Pathways: Chemokine signaling pathway, Regulation of actin cytoskeleton
• Gene Summary: This gene encodes a RAC1-specific guanine nucleotide exchange factor (GEF). GEFs mediate the exchange of guanosine diphosphate (GDP) for guanosine triphosphate (GTP). The binding of GTP induces a conformational change in RAC1 that allows downstream effectors to bind and transduce a signal. This gene thus regulates RAC1 signaling pathways that affect cell shape, migration, adhesion, growth, survival, and polarity, as well as influencing actin cytoskeletal formation, endocytosis, and membrane trafficking. This gene thus plays an important role in cell invasion, metastasis, and carcinogenesis. In addition to RAC1, the encoded protein activates additional Rho-like GTPases such as CDC42, RAC2, RAC3 and RHOA. This gene encodes multiple protein isoforms that experience a diverse array of intramolecular, protein-protein, and phosphorylation interactions as well as phosphoinositide binding. Both the longer and shorter isoforms have C-terminal Dbl homology (DH) and pleckstrin homology (PH) domains while only the longer isoforms of this gene have the N-terminal myristoylation site and the downstream N-terminal PH domain, ras-binding domain (RBD), and PSD-95/DlgA/ZO-1 (PDZ) domain. [provided by RefSeq, Jul 2017]