TIAM1 (NM_003253) Human Tagged ORF Clone

CAT#: RG220233

TIAM1 (tGFP-tagged) - Human T-cell lymphoma invasion and metastasis 1 (TIAM1)

Product Images

Circular map for RG220233

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: TIAM1
• Synonyms: TIAM-1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG220233 representing NM_003253
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAAACGCAGAAAGTCAACATGTAGAGCACGAGTTTTATGGAGAAAAGCATGCCAGCCTGGGGCGCA
  AGCACACTTCCCGCTCCCTGCGCCTCTCGCACAAGACGCGGAGGACCAGGCACGCTTCCTCGGGGAAGGT
  GATCCACAGGAACTCCGAAGTGAGCACCCGATCCAGCAGCACCCCCAGCATCCCCCAGTCCCTGGCTGAA
  AATGGCCTGGAGCCCTTCTCCCAAGATGGTACCCTAGAAGACTTCGGGAGCCCCATCTGGGTGGACCGAG
  TGGACATGGGCTTGAGACCTGTGTCTTACACTGACTCTTCTGTCACTCCCAGCGTAGACAGCAGCATCGT
  CCTCACAGCAGCCTCTGTGCAGAGCATGCCAGACACTGAGGAGAGCAGGCTTTACGGGGATGACGCTACA
  TATTTGGCTGAGGGAGGCAGGAGGCAGCATTCCTATACATCCAATGGGCCCACTTTCATGGAGACGGCGA
  GCTTTAAGAAGAAACGCTCCAAATCTGCAGACATCTGGCGGGAGGACAGCCTGGAATTCTCACTCTCTGA
  TCTGAGCCAAGAACATTTAACAAGCAACGAAGAAATCTTGGGTTCCGCCGAAGAGAAGGACTGCGAGGAG
  GCTCGGGGGATGGAAACGCGGGCGAGTCCGCGGCAGCTCAGCACCTGTCAGAGAGCCAATTCCTTGGGTG
  ACTTGTATGCTCAGAAAAACTCTGGAGTGACAGCAAACGGGGGGCCGGGGAGCAAATTTGCAGGCTACTG
  TCGGAATTTGGTGTCTGATATTCCCAATCTTGCAAACCATAAGATGCCACCAGCTGCTGCTGAAGAGACT
  CCTCCGTACAGTAATTATAACACACTTCCCTGTAGGAAATCTCACTGTCTCTCTGAAGGTGCCACCAACC
  CACAAATTAGCCATAGCAACAGCATGCAAGGCAGAAGAGCTAAAACAACTCAGGATGTTAATGCAGGCGA
  GGGCAGTGAGTTTGCAGACAGTGGGATTGAAGGGGCCACTACCGACACGGACCTCCTGTCCAGGCGATCT
  AATGCCACCAACTCCAGCTACTCACCCACCACAGGCCGGGCCTTTGTGGGCAGCGACAGCGGCAGCAGCT
  CCACCGGGGATGCGGCTCGTCAGGGGGTGTACGAGAACTTCCGGCGGGAGCTGGAGATGAGCACCACCAA
  CAGCGAGAGCCTGGAGGAGGCCGGCTCGGCGCACAGCGATGAGCAGAGCAGCGGCACCCTGAGCTCTCCG
  GGCCAGTCGGACATCCTGCTGACCGCCGCACAGGGCACGGTGCGCAAGGCCGGCGCCCTGGCCGTCAAGA
  ACTTCCTGGTGCACAAGAAGAACAAGAAGGTGGAGTCAGCCACCCGGAGGAAGTGGAAGCACTACTGGGT
  GTCCCTGAAAGGATGCACGCTATTTTTCTACGAGAGCGACGGCAGGTCTGGGATAGACCACAACAGCATC
  CCCAAACACGCCGTCTGGGTGGAGAACAGCATTGTGCAGGCGGTGCCTGAGCACCCCAAGAAGGACTTTG
  TCTTCTGCCTCAGCAATTCCCTGGGTGATGCCTTCCTTTTTCAGACCACTAGCCAGACGGAGCTTGAAAA
  CTGGATCACCGCCATCCACTCTGCCTGCGCCACTGCGGTCGCGAGGCACCACCACAAGGAAGACACGCTC
  CGACTCCTGAAATCAGAGATCAAAAAACTGGAACAGAAGATTGACATGGATGAAAAGATGAAGAAAATGG
  GTGAAATGCAGCTGTCTTCAGTCACTGACTCAAAGAAAAAGAAAACAATATTAGATCAGATCTTTGTCTG
  GGAGCAAAATCTCGAGCAGTTCCAAATGGACCTGTTTCGTTTCCGCTGTTATTTAGCCAGCCTTCAGGGT
  GGGGAGCTGCCAAACCCCAAAAGGCTTCTCGCTTTTGCAAGTCGACCAACGAAAGTGGCCATGGGCCGCC
  TTGGAATCTTTTCGGTATCATCGTTTCATGCCCTGGTGGCAGCACGCACTGGTGAAACTGGAGTGAGAAG
  ACGTACTCAGGCCATGTCCAGATCCGCGAGCAAGCGAAGGAGCAGGTTTTCTTCTCTGTGGGGTCTGGAT
  ACTACCTCCAAAAAGAAGCAGGGACGGCCAAGCATCAATCAGGTGTTTGGAGAGGGAACCGAAGCTGTAA
  AGAAATCTTTAGAGGGAATATTTGATGACATTGTTCCAGATGGCAAGAGGGAGAAAGAAGTGGTCTTACC
  TAACGTTCACCAGCACAACCCTGACTGCGACATTTGGGTCCACGAGTATTTCACTCCATCCTGGTTCTGT
  CTGCCCAATAATCAGCCTGCCCTGACGGTCGTCCGGCCAGGCGACACTGCACGGGACACCCTGGAGCTGA
  TTTGCAAGACACATCAACTGGATCATTCTGCTCATTACCTGCGCCTGAAATTTCTAATAGAAAACAAAAT
  GCAGCTCTATGTTCCACAGCCCGAGGAAGACATCTATGAGCTGCTGTACAAAGAAATTGAAATCTGTCCA
  AAAGTCACTCAGAGCATCCACATTGAGAAGTCAGATACAGCTGCTGATACTTACGGGTTTTCACTTTCTT
  CTGTGGAAGAAGATGGTATTCGAAGGCTGTACGTGAATAGTGTGAAGGAAACCGGTTTAGCTTCCAAGAA
  AGGCCTGAAAGCAGGAGATGAGATTCTTGAGATCAATAATCGTGCTGCTGACGCCCTGAACTCTTCTATG
  CTCAAAGATTTCCTCTCACAGCCCTCGCTGGGCCTCCTGGTGAGGACCTACCCCGAGCTGGAGGAAGGAG
  TGGAGCTGCTGGAAAGCCCGCCCCACCGAGTGGACGGCCCTGCCGACCTTGGCGAGAGCCCCCTCGCCTT
  TCTCACCAGCAACCCAGGGCACAGCCTTTGCAGCGAGCAGGGCAGCAGTGCTGAGACCGCTCCAGAGGAG
  ACCGAGGGGCCAGACTTGGAATCCTCAGATGAGACTGATCACAGCAGCAAGAGTACAGAACAGGTGGCCG
  CATTTTGCCGCAGTTTGCATGAGATGAACCCCTCTGACCAGAGCCCATCTCCTCAGGACTCCACGGGGCC
  TCAGCTGGCGACCATGAGACAACTCTCGGATGCAGATAAGCTGCGCAAGGTGATCTGCGAGCTCCTGGAG
  ACGGAGCGCACCTACGTGAAGGATTTAAACTGTCTTATGGAGAGATACCTAAAGCCTCTTCAAAAAGAAA
  CTTTTCTCACCCAGGATGAGCTTGACGTGCTTTTTGGAAATTTAACGGAAATGGTAGAGTTTCAAGTAGA
  ATTCCTTAAAACTCTAGAAGATGGAGTGAGACTGGTACCTGATTTGGAAAAGCTTGAGAAGGTTGATCAA
  TTTAAGAAAGTGCTGTTCTCTCTGGGGGGATCATTCCTGTATTATGCTGACCGCTTCAAGCTCTACAGTG
  CCTTCTGCGCCAGCCACACAAAAGTTCCCAAGGTCCTGGTGAAAGCCAAGACAGACACGGCTTTCAAGGC
  ATTCTTGGATGCCCAGAACCCGAAGCAGCAGCACTCATCCACGCTGGAGTCGTACCTCATCAAGCCCATC
  CAGAGGATCCTCAAGTACCCACTTCTGCTCAGGGAGCTGTTCGCCCTGACCGATGCGGAGAGCGAGGAGC
  ACTACCACCTGGACGTGGCCATCAAGACCATGAACAAGGTTGCCAGTCACATCAATGAGATGCAGAAAAT
  CCATGAAGAGTTTGGGGCTGTGTTTGACCAGCTGATTGCTGAACAGACTGGTGAGAAAAAAGAGGTTGCA
  GATCTGAGCATGGGAGACCTGCTTTTGCACACTACCGTGATCTGGCTGAACCCGCCGGCCTCGCTGGGCA
  AGTGGAAAAAGGAACCAGAGTTGGCAGCATTCGTCTTCAAAACTGCTGTGGTCCTTGTGTATAAAGATGG
  TTCCAAACAGAAGAAGAAACTTGTAGGATCTCACAGGCTTTCCATTTATGAGGACTGGGACCCCTTCAGA
  TTTCGACACATGATCCCCACGGAAGCGCTGCAGGTTCGAGCTTTGGCGAGTGCAGATGCAGAGGCAAATG
  CCGTGTGTGAAATTGTCCATGTAAAATCCGAGTCTGAAGGGAGGCCGGAGAGGGTCTTTCACTTGTGCTG
  CAGCTCCCCAGAGAGCCGAAAGGATTTCCTAAAGGCTGTGCATTCAATCCTGCGTGATAAGCACAGAAGA
  CAGCTCCTCAAAACCGAGAGCCTTCCCTCATCCCAGCAATATGTCCCTTTTGGAGGCAAAAGATTGTGTG
  CACTGAAGGGGGCCAGGCCGGCCATGAGCAGGGCAGTGTCTGCCCCAAGCAAGTCTCTTGGGAGGAGGAG
  GCGGCGGCTGGCTCGAAACAGGTTTACCATTGATTCTGATGCCGTCTCCGCAAGCAGCCCGGAGAAAGAG
  TCCCAGCAGCCCCCCGGTGGTGGGGACACTGACCGATGGGTAGAGGAGCAGTTTGATCTTGCTCAGTATG
  AGGAGCAAGATGACATCAAGGAGACAGACATCCTCAGTGACGATGATGAGTTCTGTGAGTCCGTGAAGGG
  TGCCTCAGTGGACAGAGACCTGCAGGAGCGGCTTCAGGCCACCTCCATCAGTCAGCGGGAAAGAGGCCGG
  AAAACCCTGGATAGTCACGCGTCCCGCATGGCACAGCTCAAGAAGCAAGCTGCCCTGTCGGGGATCAATG
  GAGGCCTGGAGAGCGCAAGCGAGGAAGTCATTTGGGTTAGGCGTGAAGACTTTGCCCCCTCCAGGAAACT
  GAACACTGAGATC
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG220233 representing NM_003253
  Red=Cloning site Green=Tags(s)
  
  MGNAESQHVEHEFYGEKHASLGRKHTSRSLRLSHKTRRTRHASSGKVIHRNSEVSTRSSSTPSIPQSLAE
  NGLEPFSQDGTLEDFGSPIWVDRVDMGLRPVSYTDSSVTPSVDSSIVLTAASVQSMPDTEESRLYGDDAT
  YLAEGGRRQHSYTSNGPTFMETASFKKKRSKSADIWREDSLEFSLSDLSQEHLTSNEEILGSAEEKDCEE
  ARGMETRASPRQLSTCQRANSLGDLYAQKNSGVTANGGPGSKFAGYCRNLVSDIPNLANHKMPPAAAEET
  PPYSNYNTLPCRKSHCLSEGATNPQISHSNSMQGRRAKTTQDVNAGEGSEFADSGIEGATTDTDLLSRRS
  NATNSSYSPTTGRAFVGSDSGSSSTGDAARQGVYENFRRELEMSTTNSESLEEAGSAHSDEQSSGTLSSP
  GQSDILLTAAQGTVRKAGALAVKNFLVHKKNKKVESATRRKWKHYWVSLKGCTLFFYESDGRSGIDHNSI
  PKHAVWVENSIVQAVPEHPKKDFVFCLSNSLGDAFLFQTTSQTELENWITAIHSACATAVARHHHKEDTL
  RLLKSEIKKLEQKIDMDEKMKKMGEMQLSSVTDSKKKKTILDQIFVWEQNLEQFQMDLFRFRCYLASLQG
  GELPNPKRLLAFASRPTKVAMGRLGIFSVSSFHALVAARTGETGVRRRTQAMSRSASKRRSRFSSLWGLD
  TTSKKKQGRPSINQVFGEGTEAVKKSLEGIFDDIVPDGKREKEVVLPNVHQHNPDCDIWVHEYFTPSWFC
  LPNNQPALTVVRPGDTARDTLELICKTHQLDHSAHYLRLKFLIENKMQLYVPQPEEDIYELLYKEIEICP
  KVTQSIHIEKSDTAADTYGFSLSSVEEDGIRRLYVNSVKETGLASKKGLKAGDEILEINNRAADALNSSM
  LKDFLSQPSLGLLVRTYPELEEGVELLESPPHRVDGPADLGESPLAFLTSNPGHSLCSEQGSSAETAPEE
  TEGPDLESSDETDHSSKSTEQVAAFCRSLHEMNPSDQSPSPQDSTGPQLATMRQLSDADKLRKVICELLE
  TERTYVKDLNCLMERYLKPLQKETFLTQDELDVLFGNLTEMVEFQVEFLKTLEDGVRLVPDLEKLEKVDQ
  FKKVLFSLGGSFLYYADRFKLYSAFCASHTKVPKVLVKAKTDTAFKAFLDAQNPKQQHSSTLESYLIKPI
  QRILKYPLLLRELFALTDAESEEHYHLDVAIKTMNKVASHINEMQKIHEEFGAVFDQLIAEQTGEKKEVA
  DLSMGDLLLHTTVIWLNPPASLGKWKKEPELAAFVFKTAVVLVYKDGSKQKKKLVGSHRLSIYEDWDPFR
  FRHMIPTEALQVRALASADAEANAVCEIVHVKSESEGRPERVFHLCCSSPESRKDFLKAVHSILRDKHRR
  QLLKTESLPSSQQYVPFGGKRLCALKGARPAMSRAVSAPSKSLGRRRRRLARNRFTIDSDAVSASSPEKE
  SQQPPGGGDTDRWVEEQFDLAQYEEQDDIKETDILSDDDEFCESVKGASVDRDLQERLQATSISQRERGR
  KTLDSHASRMAQLKKQAALSGINGGLESASEEVIWVRREDFAPSRKLNTEI
  
  SGPTRTRRLE - GFP Tag - V
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003253
• ORF Size: 4773 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003253.3
• RefSeq Size: 7218 bp
• RefSeq ORF: 4776 bp
• Locus ID: 7074
• UniProt ID: Q13009
• Cytogenetics: 21q22.11
• Domains: RhoGEF, PDZ, PH, RBD
• Protein Families: Druggable Genome
• Protein Pathways: Chemokine signaling pathway, Regulation of actin cytoskeleton
• Gene Summary: This gene encodes a RAC1-specific guanine nucleotide exchange factor (GEF). GEFs mediate the exchange of guanosine diphosphate (GDP) for guanosine triphosphate (GTP). The binding of GTP induces a conformational change in RAC1 that allows downstream effectors to bind and transduce a signal. This gene thus regulates RAC1 signaling pathways that affect cell shape, migration, adhesion, growth, survival, and polarity, as well as influencing actin cytoskeletal formation, endocytosis, and membrane trafficking. This gene thus plays an important role in cell invasion, metastasis, and carcinogenesis. In addition to RAC1, the encoded protein activates additional Rho-like GTPases such as CDC42, RAC2, RAC3 and RHOA. This gene encodes multiple protein isoforms that experience a diverse array of intramolecular, protein-protein, and phosphorylation interactions as well as phosphoinositide binding. Both the longer and shorter isoforms have C-terminal Dbl homology (DH) and pleckstrin homology (PH) domains while only the longer isoforms of this gene have the N-terminal myristoylation site and the downstream N-terminal PH domain, ras-binding domain (RBD), and PSD-95/DlgA/ZO-1 (PDZ) domain. [provided by RefSeq, Jul 2017]