BRDT (NM_207189) Human Untagged Clone

CAT#: SC308409

BRDT (untagged)-Human bromodomain, testis-specific (BRDT), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: BRDT
• Synonyms: BRD6; CT9; SPGF21
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC308409 representing NM_207189.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGTCTCTGCCAAGTCGACAAACAGCTATTATTGTTAACCCTCCTCCACCAGAATATATAAATACTAAG
  AAAAATGGGCGATTGACAAATCAACTTCAGTATCTACAAAAAGTTGTCCTAAAGGATTTATGGAAGCAT
  AGTTTTTCATGGCCCTTTCAACGTCCTGTGGATGCTGTGAAACTACAGTTGCCTGATTATTATACCATT
  ATAAAAAACCCAATGGATTTAAATACAATTAAGAAGCGCTTGGAGAATAAATATTATGCGAAGGCTTCA
  GAATGTATAGAAGACTTCAATACAATGTTCTCAAATTGTTATTTATATAACAAGCCTGGAGATGACATT
  GTTCTTATGGCACAAGCTCTAGAGAAGCTGTTTATGCAGAAATTATCTCAGATGCCACAAGAAGAGCAA
  GTTGTGGGTGTTAAGGAAAGAATCAAGAAAGGCACTCAACAGAATATAGCTGTTTCTTCTGCTAAAGAA
  AAATCATCACCCAGCGCAACAGAAAAAGTATTTAAGCAGCAAGAAATTCCTTCTGTATTTCCTAAGACA
  TCTATTTCTCCCTTGAACGTGGTACAGGGAGCTTCAGTCAACTCCAGTTCACAAACTGCGGCCCAAGTT
  ACAAAAGGTGTGAAGAGGAAAGCAGATACAACAACTCCTGCAACTTCAGCAGTTAAAGCAAGTAGTGAA
  TTTTCTCCAACATTCACAGAAAAATCAGTGGCACTGCCACCTATAAAAGAAAATATGCCAAAGAATGTT
  TTGCCAGATTCTCAGCAACAATATAATGTTGTGAAGACTGTTAAAGTAACTGAACAATTAAGGCACTGT
  AGTGAGATTCTTAAAGAAATGCTTGCAAAGAAACATTTTTCATATGCATGGCCCTTTTATAATCCTGTT
  GACGTTAATGCTTTGGGACTCCATAACTACTATGACGTTGTCAAAAATCCGATGGATCTTGGAACTATT
  AAGGAGAAAATGGATAACCAAGAATATAAGGATGCATACAAATTTGCGGCAGATGTTAGATTAATGTTC
  ATGAATTGCTACAAGTACAATCCTCCAGATCACGAAGTTGTGACAATGGCAAGAATGCTTCAGGATGTT
  TTCGAAACGCATTTTTCAAAGATCCCGATTGAACCTGTTGAGAGTATGCCTTTATGTTACATCAAAACA
  GATATCACAGAAACCACTGGTAGAGAGAACACTAATGAAGCCTCCTCTGAAGGGAACTCTTCTGATGAT
  TCTGAAGATGAGCGAGTTAAGCGTCTTGCAAAGCTTCAGGAGCAGCTTAAAGCTGTACATCAACAGCTC
  CAGGTTTTGTCCCAAGTACCTTTCCGTAAGCTAAATAAAAAGAAAGAGAAGTCTAAAAAGGAAAAGAAA
  AAAGAAAAGGTTAATAACAGCAATGAAAATCCAAGAAAAATGTGTGAGCAAATGAGGCTAAAGGAAAAG
  TCCAAGAGAAATCAGCCAAAGAAAAGGAAACAACAGTTCATTGGTCTAAAATCTGAAGATGAAGATAAT
  GCTAAACCTATGAACTATGATGAGAAAAGGCAGTTAAGTCTGAATATAAACAAACTCCCTGGAGATAAA
  CTTGGGCGAGTAGTTCACATAATACAATCAAGAGAGCCTTCTCTGAGCAATTCCAATCCTGATGAGATA
  GAGATAGACTTTGAAACACTGAAAGCATCAACACTAAGAGAATTAGAAAAATATGTTTCGGCATGTCTA
  AGAAAGAGACCATTAAAACCTCCTGCTAAGAAAATAATGATGTCCAAAGAAGAACTTCACTCACAGAAA
  AAACAGGAATTGGAAAAGCGGTTACTGGATGTTAATAATCAGTTAAATTCTAGAAAACGTCAAACAAAA
  TCTGATAAAACGCAACCATCCAAAGCTGTTGAAAATGTTTCCCGACTGAGTGAGAGCAGCAGCAGCAGC
  AGCAGCTCATCAGAGTCTGAAAGTAGCAGCAGTGACTTAAGCTCTTCAGACAGCAGTGATTCTGAATCA
  GAAATGTTCCCTAAGTTTACAGAAGTAAAACCAAATGATTCTCCTTCTAAAGAGAATGTAAAGAAAATG
  AAGAATGAATGCATACCGCCTGAAGGAAGAACAGGCGTCACACAGATAGGATATTGTGTGCAAGACACA
  ACCTCTGCCAATACTACCCTTGTTCATCAGACCACACCTTCACATGTAATGCCACCAAATCACCACCAA
  TTAGCATTTAATTATCAAGAATTAGAACATTTACAGACTGTGAAAAACATTTCACCTTTACAAATTCTG
  CCTCCCTCAGGTGATTCTGAACAGCTCTCAAATGGCATAACTGTGATGCATCCATCTGGTGATAGTGAC
  ACAACGATGTTAGAATCTGAATGTCAAGCTCCTGTACAGAAGGATATAAAGATTAAGAATGCAGATTCA
  TGGAAAAGTTTAGGCAAACCAGTGAAACCATCAGGTGTAATGAAATCCTCAGATGAGCTCTTCAACCAA
  TTTAGAAAAGCAGCCATAGAAAAGGAAGTAAAAGCTCGGACACAGGAACTCATACGGAAGCATTTGGAA
  CAAAATACAAAGGAACTAAAAGCATCTCAAGAAAATCAGAGGGATCTTGGGAATGGATTGACTGTAGAA
  TCTTTTTCAAATAAAATACAAAACAAGTGCTCTGGAGAAGAGCAGAAAGAACATCAGCAGTCATCAGAA
  GCTCAAGATAAATCCAAACTCTGGCTTCTCAAAGACCGTGATTTAGCAAGGCAGAAAGAACAAGAGAGG
  AGGAGGAGAGAAGCAATGGTGGGTACCATTGATATGACCCTTCAAAGTGACATTATGACAATGTTTGAA
  AACAACTTTGATTAA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_207189
• Insert Size: 2844 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_207189.3
• RefSeq Size: 3361 bp
• RefSeq ORF: 2844 bp
• Locus ID: 676
• UniProt ID: Q58F21
• Cytogenetics: 1p22.1
• Protein Families: Protein Kinase, Transcription Factors
• MW: 108 kDa
• Gene Summary: BRDT is similar to the RING3 protein family. It possesses 2 bromodomain motifs and a PEST sequence (a cluster of proline, glutamic acid, serine, and threonine residues), characteristic of proteins that undergo rapid intracellular degradation. The bromodomain is found in proteins that regulate transcription. Several transcript variants encoding multiple isoforms have been found for this gene. [provided by RefSeq, Jun 2011]
  Transcript Variant: This variant (1) differs in the 5' UTR and uses an alternate in-frame splice junction at the 3' end of an exon compared to variant 4. The resulting isoform (b) lacks a short internal segment compared to isoform a. Variants 1, 2, and 3 all encode the same isoform (b).