G3BP2 (NM_203504) Human Untagged Clone

CAT#: SC308217

G3BP2 (untagged)-Human GTPase activating protein (SH3 domain) binding protein 2 (G3BP2), transcript variant 3

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: G3BP2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC308217 representing NM_203504.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGTTATGGAGAAGCCCAGTCCGCTGCTTGTAGGGCGGGAGTTTGTGAGGCAATATTATACTTTGCTG
  AATAAAGCTCCGGAATATTTACACAGGTTTTATGGCAGGAATTCTTCCTATGTTCATGGTGGAGTAGAT
  GCTAGTGGAAAGCCCCAGGAAGCTGTTTATGGCCAAAATGATATACACCACAAAGTATTATCTCTGAAC
  TTCAGTGAATGTCATACTAAAATTCGTCATGTGGATGCTCATGCAACCTTGAGTGATGGAGTAGTTGTC
  CAGGTCATGGGTTTGCTGTCTAACAGTGGACAACCAGAAAGAAAGTTTATGCAAACCTTTGTTCTGGCT
  CCTGAAGGATCTGTTCCAAATAAATTTTATGTTCACAATGATATGTTTCGTTATGAAGATGAAGTGTTT
  GGTGATTCTGAGCCTGAACTTGATGAAGAATCAGAAGATGAAGTAGAAGAGGAACAAGAAGAAAGACAA
  CCATCTCCTGAACCTGTGCAAGAAAATGCTAACAGTGGTTACTATGAAGCTCACCCTGTGACTAATGGC
  ATAGAGGAGCCTTTGGAAGAATCCTCTCATGAACCTGAACCTGAGCCAGAATCTGAAACAAAGACTGAA
  GAGCTGAAACCACAAGTGGAGGAGAAGAACTTAGAAGAACTAGAGGAGAAATCTACTACTCCTCCTCCG
  GCAGAACCTGTTTCTCTGCCACAAGAACCACCAAAGCCAAGAGTCGAAGCTAAACCAGAAGTTCAATCT
  CAGCCACCTCGTGTGCGTGAACAACGACCTAGAGAACGACCTGGTTTTCCTCCTAGAGGACCAAGACCA
  GGCAGAGGAGATATGGAACAGAATGACTCTGACAACCGTAGAATAATTCGCTATCCAGATAGTCATCAA
  CTTTTTGTTGGTAACTTGCCACATGATATTGATGAAAATGAGCTAAAGGAATTCTTCATGAGTTTTGGA
  AACGTTGTGGAACTTCGCATCAATACCAAGGGTGTTGGGGGAAAGCTTCCAAATTTTGGTTTTGTGGTT
  TTTGATGACTCTGAACCAGTTCAGAGAATCTTAATTGCAAAACCGATTATGTTTCGAGGGGAAGTACGT
  TTAAATGTGGAAGAGAAAAAAACAAGAGCTGCAAGAGAGCGAGAAACCAGAGGTGGTGGTGATGATCGC
  AGGGATATTAGGCGCAATGATCGAGGTCCCGGTGGTCCACGTGGAATTGTGGGTGGTGGAATGATGCGT
  GATCGTGATGGAAGAGGACCTCCTCCAAGGGGTGGCATGGCACAGAAACTTGGCTCTGGAAGAGGAACC
  GGGCAAATGGAGGGCCGCTTCACAGGACAGCGTCGCTGA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_203504
• Insert Size: 1350 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_203504.2
• RefSeq Size: 4311 bp
• RefSeq ORF: 1350 bp
• Locus ID: 9908
• UniProt ID: Q9UN86
• Cytogenetics: 4q21.1
• Protein Families: Druggable Genome
• MW: 50.8 kDa
• Gene Summary: Scaffold protein that plays an essential role in cytoplasmic stress granule formation which acts as a platform for antiviral signaling.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) lacks an alternate in-frame exon compared to variant 1. The resulting isoform (b) has the same N- and C-termini but is shorter compared to isoform a.