G3BP2 (NM_203504) Human Tagged ORF Clone

CAT#: RG203977

G3BP2 (tGFP-tagged) - Human GTPase activating protein (SH3 domain) binding protein 2 (G3BP2), transcript variant 3

Product Images

Circular map for RG203977

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: G3BP2
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG203977 representing NM_203504
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTTATGGAGAAGCCCAGTCCGCTGCTTGTAGGGCGGGAGTTTGTGAGGCAATATTATACTTTGCTGA
  ATAAAGCTCCGGAATATTTACACAGGTTTTATGGCAGGAATTCTTCCTATGTTCATGGTGGAGTAGATGC
  TAGTGGAAAGCCCCAGGAAGCTGTTTATGGCCAAAATGATATACACCACAAAGTATTATCTCTGAACTTC
  AGTGAATGTCATACTAAAATTCGTCATGTGGATGCTCATGCAACCTTGAGTGATGGAGTAGTTGTCCAGG
  TCATGGGTTTGCTGTCTAACAGTGGACAACCAGAAAGAAAGTTTATGCAAACCTTTGTTCTGGCTCCTGA
  AGGATCTGTTCCAAATAAATTTTATGTTCACAATGATATGTTTCGTTATGAAGATGAAGTGTTTGGTGAT
  TCTGAGCCTGAACTTGATGAAGAATCAGAAGATGAAGTAGAAGAGGAACAAGAAGAAAGACAACCATCTC
  CTGAACCTGTGCAAGAAAATGCTAACAGTGGTTACTATGAAGCTCACCCTGTGACTAATGGCATAGAGGA
  GCCTTTGGAAGAATCCTCTCATGAACCTGAACCTGAGCCAGAATCTGAAACAAAGACTGAAGAGCTGAAA
  CCACAAGTGGAGGAGAAGAACTTAGAAGAACTAGAGGAGAAATCTACTACTCCTCCTCCGGCAGAACCTG
  TTTCTCTGCCACAAGAACCACCAAAGCCAAGAGTCGAAGCTAAACCAGAAGTTCAATCTCAGCCACCTCG
  TGTGCGTGAACAACGACCTAGAGAACGACCTGGTTTTCCTCCTAGAGGACCAAGACCAGGCAGAGGAGAT
  ATGGAACAGAATGACTCTGACAACCGTAGAATAATTCGCTATCCAGATAGTCATCAACTTTTTGTTGGTA
  ACTTGCCACATGATATTGATGAAAATGAGCTAAAGGAATTCTTCATGAGTTTTGGAAACGTTGTGGAACT
  TCGCATCAATACCAAGGGTGTTGGGGGAAAGCTTCCAAATTTTGGTTTTGTGGTTTTTGATGACTCTGAA
  CCAGTTCAGAGAATCTTAATTGCAAAACCGATTATGTTTCGAGGGGAAGTACGTTTAAATGTGGAAGAGA
  AAAAAACAAGAGCTGCAAGAGAGCGAGAAACCAGAGGTGGTGGTGATGATCGCAGGGATATTAGGCGCAA
  TGATCGAGGTCCCGGTGGTCCACGTGGAATTGTGGGTGGTGGAATGATGCGTGATCGTGATGGAAGAGGA
  CCTCCTCCAAGGGGTGGCATGGCACAGAAACTTGGCTCTGGAAGAGGAACCGGGCAAATGGAGGGCCGCT
  TCACAGGACAGCGTCGC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG203977 representing NM_203504
  Red=Cloning site Green=Tags(s)
  
  MVMEKPSPLLVGREFVRQYYTLLNKAPEYLHRFYGRNSSYVHGGVDASGKPQEAVYGQNDIHHKVLSLNF
  SECHTKIRHVDAHATLSDGVVVQVMGLLSNSGQPERKFMQTFVLAPEGSVPNKFYVHNDMFRYEDEVFGD
  SEPELDEESEDEVEEEQEERQPSPEPVQENANSGYYEAHPVTNGIEEPLEESSHEPEPEPESETKTEELK
  PQVEEKNLEELEEKSTTPPPAEPVSLPQEPPKPRVEAKPEVQSQPPRVREQRPRERPGFPPRGPRPGRGD
  MEQNDSDNRRIIRYPDSHQLFVGNLPHDIDENELKEFFMSFGNVVELRINTKGVGGKLPNFGFVVFDDSE
  PVQRILIAKPIMFRGEVRLNVEEKKTRAARERETRGGGDDRRDIRRNDRGPGGPRGIVGGGMMRDRDGRG
  PPPRGGMAQKLGSGRGTGQMEGRFTGQRR
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_203504
• ORF Size: 1347 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_203504.2, NP_987100.1
• RefSeq Size: 4254 bp
• RefSeq ORF: 1350 bp
• Locus ID: 9908
• UniProt ID: Q9UN86
• Cytogenetics: 4q21.1
• Protein Families: Druggable Genome
• Gene Summary: Scaffold protein that plays an essential role in cytoplasmic stress granule formation which acts as a platform for antiviral signaling.[UniProtKB/Swiss-Prot Function]