KIAA1199 (CEMIP) (NM_018689) Human Untagged Clone

CAT#: SC304626

KIAA1199 (untagged)-Human KIAA1199 (KIAA1199)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: KIAA1199
• Synonyms: CCSP1; HYBID; KIAA1199; TMEM2L
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC304626 representing NM_018689.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGGAGCTGCTGGGAGGCAGGACTTCCTCTTCAAGGCCATGCTGACCATCAGCTGGCTCACTCTGACC
  TGCTTCCCTGGGGCCACATCCACAGTGGCTGCTGGGTGCCCTGACCAGAGCCCTGAGTTGCAACCCTGG
  AACCCTGGCCATGACCAAGACCACCATGTGCATATCGGCCAGGGCAAGACACTGCTGCTCACCTCTTCT
  GCCACGGTCTATTCCATCCACATCTCAGAGGGAGGCAAGCTGGTCATTAAAGACCACGACGAGCCGATT
  GTTTTGCGAACCCGGCACATCCTGATTGACAACGGAGGAGAGCTGCATGCTGGGAGTGCCCTCTGCCCT
  TTCCAGGGCAATTTCACCATCATTTTGTATGGAAGGGCTGATGAAGGTATTCAGCCGGATCCTTACTAT
  GGTCTGAAGTACATTGGGGTTGGTAAAGGAGGCGCTCTTGAGTTGCATGGACAGAAAAAGCTCTCCTGG
  ACATTTCTGAACAAGACCCTTCACCCAGGTGGCATGGCAGAAGGAGGCTATTTTTTTGAAAGGAGCTGG
  GGCCACCGTGGAGTTATTGTTCATGTCATCGACCCCAAATCAGGCACAGTCATCCATTCTGACCGGTTT
  GACACCTATAGATCCAAGAAAGAGAGTGAACGTCTGGTCCAGTATTTGAACGCGGTGCCCGATGGCAGG
  ATCCTTTCTGTTGCAGTGAATGATGAAGGTTCTCGAAATCTGGATGACATGGCCAGGAAGGCGATGACC
  AAATTGGGAAGCAAACACTTCCTGCACCTTGGATTTAGACACCCTTGGAGTTTTCTAACTGTGAAAGGA
  AATCCATCATCTTCAGTGGAAGACCATATTGAATATCATGGACATCGAGGCTCTGCTGCTGCCCGGGTA
  TTCAAATTGTTCCAGACAGAGCATGGCGAATATTTCAATGTTTCTTTGTCCAGTGAGTGGGTTCAAGAC
  GTGGAGTGGACGGAGTGGTTCGATCATGATAAAGTATCTCAGACTAAAGGTGGGGAGAAAATTTCAGAC
  CTCTGGAAAGCTCACCCAGGAAAAATATGCAATCGTCCCATTGATATACAGGCCACTACAATGGATGGA
  GTTAACCTCAGCACCGAGGTTGTCTACAAAAAAGGCCAGGATTATAGGTTTGCTTGCTACGACCGGGGC
  AGAGCCTGCCGGAGCTACCGTGTACGGTTCCTCTGTGGGAAGCCTGTGAGGCCCAAACTCACAGTCACC
  ATTGACACCAATGTGAACAGCACCATTCTGAACTTGGAGGATAATGTACAGTCATGGAAACCTGGAGAT
  ACCCTGGTCATTGCCAGTACTGATTACTCCATGTACCAGGCAGAAGAGTTCCAGGTGCTTCCCTGCAGA
  TCCTGCGCCCCCAACCAGGTCAAAGTGGCAGGGAAACCAATGTACCTGCACATCGGGGAGGAGATAGAC
  GGCGTGGACATGCGGGCGGAGGTTGGGCTTCTGAGCCGGAACATCATAGTGATGGGGGAGATGGAGGAC
  AAATGCTACCCCTACAGAAACCACATCTGCAATTTCTTTGACTTCGATACCTTTGGGGGCCACATCAAG
  TTTGCTCTGGGATTTAAGGCAGCACACTTGGAGGGCACGGAGCTGAAGCATATGGGACAGCAGCTGGTG
  GGTCAGTACCCGATTCACTTCCACCTGGCCGGTGATGTAGACGAAAGGGGAGGTTATGACCCACCCACA
  TACATCAGGGACCTCTCCATCCATCATACATTCTCTCGCTGCGTCACAGTCCATGGCTCCAATGGCTTG
  TTGATCAAGGACGTTGTGGGCTATAACTCTTTGGGCCACTGCTTCTTCACGGAAGATGGGCCGGAGGAA
  CGCAACACTTTTGACCACTGTCTTGGCCTCCTTGTCAAGTCTGGAACCCTCCTCCCCTCGGACCGTGAC
  AGCAAGATGTGCAAGATGATCACAGAGGACTCCTACCCGGGGTACATCCCCAAGCCCAGGCAAGACTGC
  AATGCTGTGTCCACCTTCTGGATGGCCAATCCCAACAACAACCTCATCAACTGTGCCGCTGCAGGATCT
  GAGGAAACTGGATTTTGGTTTATTTTTCACCACGTACCAACGGGCCCCTCCGTGGGAATGTACTCCCCA
  GGTTATTCAGAGCACATTCCACTGGGAAAATTCTATAACAACCGAGCACATTCCAACTACCGGGCTGGC
  ATGATCATAGACAACGGAGTCAAAACCACCGAGGCCTCTGCCAAGGACAAGCGGCCGTTCCTCTCAATC
  ATCTCTGCCAGATACAGCCCTCACCAGGACGCCGACCCGCTGAAGCCCCGGGAGCCGGCCATCATCAGA
  CACTTCATTGCCTACAAGAACCAGGACCACGGGGCCTGGCTGCGCGGCGGGGATGTGTGGCTGGACAGC
  TGCCGGTTTGCTGACAATGGCATTGGCCTGACCCTGGCCAGTGGTGGAACCTTCCCGTATGACGACGGC
  TCCAAGCAAGAGATAAAGAACAGCTTGTTTGTTGGCGAGAGTGGCAACGTGGGGACGGAAATGATGGAC
  AATAGGATCTGGGGCCCTGGCGGCTTGGACCATAGCGGAAGGACCCTCCCTATAGGCCAGAATTTTCCA
  ATTAGAGGAATTCAGTTATATGATGGCCCCATCAACATCCAAAACTGCACTTTCCGAAAGTTTGTGGCC
  CTGGAGGGCCGGCACACCAGCGCCCTGGCCTTCCGCCTGAATAATGCCTGGCAGAGCTGCCCCCATAAC
  AACGTGACCGGCATTGCCTTTGAGGACGTTCCGATTACTTCCAGAGTGTTCTTCGGAGAGCCTGGGCCC
  TGGTTCAACCAGCTGGACATGGATGGGGATAAGACATCTGTGTTCCATGACGTCGACGGCTCCGTGTCC
  GAGTACCCTGGCTCCTACCTCACGAAGAATGACAACTGGCTGGTCCGGCACCCAGACTGCATCAATGTT
  CCCGACTGGAGAGGGGCCATTTGCAGTGGGTGCTATGCACAGATGTACATTCAAGCCTACAAGACCAGT
  AACCTGCGAATGAAGATCATCAAGAATGACTTCCCCAGCCACCCTCTTTACCTGGAGGGGGCGCTCACC
  AGGAGCACCCATTACCAGCAATACCAACCGGTTGTCACCCTGCAGAAGGGCTACACCATCCACTGGGAC
  CAGACGGCCCCCGCCGAACTCGCCATCTGGCTCATCAACTTCAACAAGGGCGACTGGATCCGAGTGGGG
  CTCTGCTACCCGCGAGGCACCACATTCTCCATCCTCTCGGATGTTCACAATCGCCTGCTGAAGCAAACG
  TCCAAGACGGGCGTCTTCGTGAGGACCTTGCAGATGGACAAAGTGGAGCAGAGCTACCCTGGCAGGAGC
  CACTACTACTGGGACGAGGACTCAGGGCTGTTGTTCCTGAAGCTGAAAGCTCAGAACGAGAGAGAGAAG
  TTTGCTTTCTGCTCCATGAAAGGCTGTGAGAGGATAAAGATTAAAGCTCTGATTCCAAAGAACGCAGGC
  GTCAGTGACTGCACAGCCACAGCTTACCCCAAGTTCACCGAGAGGGCTGTCGTAGACGTGCCGATGCCC
  AAGAAGCTCTTTGGTTCTCAGCTGAAAACAAAGGACCATTTCTTGGAGGTGAAGATGGAGAGTTCCAAG
  CAGCACTTCTTCCACCTCTGGAACGACTTCGCTTACATTGAAGTGGATGGGAAGAAGTACCCCAGTTCG
  GAGGATGGCATCCAGGTGGTGGTGATTGACGGGAACCAAGGGCGCGTGGTGAGCCACACGAGCTTCAGG
  AACTCCATTCTGCAAGGCATACCATGGCAGCTTTTCAACTATGTGGCGACCATCCCTGACAATTCCATA
  GTGCTTATGGCATCAAAGGGAAGATACGTCTCCAGAGGCCCATGGACCAGAGTGCTGGAAAAGCTTGGG
  GCAGACAGGGGTCTCAAGTTGAAAGAGCAAATGGCATTCGTTGGCTTCAAAGGCAGCTTCCGGCCCATC
  TGGGTGACACTGGACACTGAGGATCACAAAGCCAAAATCTTCCAAGTTGTGCCCATCCCTGTGGTGAAG
  AAGAAGAAGTTGTGA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_018689
• Insert Size: 4086 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018689.2
• RefSeq Size: 7128 bp
• RefSeq ORF: 4086 bp
• Locus ID: 57214
• UniProt ID: Q8WUJ3
• Cytogenetics: 15q25.1
• MW: 153 kDa
• Gene Summary: Mediates depolymerization of hyaluronic acid (HA) via the cell membrane-associated clathrin-coated pit endocytic pathway. Binds to hyaluronic acid. Hydrolyzes high molecular weight hyaluronic acid to produce an intermediate-sized product, a process that may occur through rapid vesicle endocytosis and recycling without intracytoplasmic accumulation or digestion in lysosomes. Involved in hyaluronan catabolism in the dermis of the skin and arthritic synovium. Positively regulates epithelial-mesenchymal transition (EMT), and hence tumor cell growth, invasion and cancer dissemination. In collaboration with HSPA5/BIP, promotes cancer cell migration in a calcium and PKC-dependent manner. May be involved in hearing.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (3) differs in the 5' UTR, compared to variant 1. Variants 1, 2, and 3 encode the same protein.