KIAA1199 (CEMIP) (NM_018689) Human Tagged ORF Clone

CAT#: RG215547

KIAA1199 (tGFP-tagged) - Human KIAA1199 (KIAA1199)

Product Images

Circular map for RG215547

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: KIAA1199
• Synonyms: CCSP1; HYBID; KIAA1199; TMEM2L
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG215547 representing NM_018689
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAGCTGCTGGGAGGCAGGACTTCCTCTTCAAGGCCATGCTGACCATCAGCTGGCTCACTCTGACCT
  GCTTCCCTGGGGCCACATCCACAGTGGCTGCTGGGTGCCCTGACCAGAGCCCTGAGTTGCAACCCTGGAA
  CCCTGGCCATGACCAAGACCACCATGTGCATATCGGCCAGGGCAAGACACTGCTGCTCACCTCTTCTGCC
  ACGGTCTATTCCATCCACATCTCAGAGGGAGGCAAGCTGGTCATTAAAGACCACGACGAGCCGATTGTTT
  TGCGAACCCGGCACATCCTGATTGACAACGGAGGAGAGCTGCATGCTGGGAGTGCCCTCTGCCCTTTCCA
  GGGCAATTTCACCATCATTTTGTATGGAAGGGCTGATGAAGGTATTCAGCCGGATCCTTACTATGGTCTG
  AAGTACATTGGGGTTGGTAAAGGAGGCGCTCTTGAGTTGCATGGACAGAAAAAGCTCTCCTGGACATTTC
  TGAACAAGACCCTTCACCCAGGTGGCATGGCAGAAGGAGGCTATTTTTTTGAAAGGAGCTGGGGCCACCG
  TGGAGTTATTGTTCATGTCATCGACCCCAAATCAGGCACAGTCATCCATTCTGACCGGTTTGACACCTAT
  AGATCCAAGAAAGAGAGTGAACGTCTGGTCCAGTATTTGAACGCGGTGCCCGATGGCAGGATCCTTTCTG
  TTGCAGTGAATGATGAAGGTTCTCGAAATCTGGATGACATGGCCAGGAAGGCGATGACCAAATTGGGAAG
  CAAACACTTCCTGCACCTTGGATTTAGACACCCTTGGAGTTTTCTAACTGTGAAAGGAAATCCATCATCT
  TCAGTGGAAGACCATATTGAATATCATGGACATCGAGGCTCTGCTGCTGCCCGGGTATTCAAATTGTTCC
  AGACAGAGCATGGCGAATATTTCAATGTTTCTTTGTCCAGTGAGTGGGTTCAAGACGTGGAGTGGACGGA
  GTGGTTCGATCATGATAAAGTATCTCAGACTAAAGGTGGGGAGAAAATTTCAGACCTCTGGAAAGCTCAC
  CCAGGAAAAATATGCAATCGTCCCATTGATATACAGGCCACTACAATGGATGGAGTTAACCTCAGCACCG
  AGGTTGTCTACAAAAAAGGCCAGGATTATAGGTTTGCTTGCTACGACCGGGGCAGAGCCTGCCGGAGCTA
  CCGTGTACGGTTCCTCTGTGGGAAGCCTGTGAGGCCCAAACTCACAGTCACCATTGACACCAATGTGAAC
  AGCACCATTCTGAACTTGGAGGATAATGTACAGTCATGGAAACCTGGAGATACCCTGGTCATTGCCAGTA
  CTGATTACTCCATGTACCAGGCAGAAGAGTTCCAGGTGCTTCCCTGCAGATCCTGCGCCCCCAACCAGGT
  CAAAGTGGCAGGGAAACCAATGTACCTGCACATCGGGGAGGAGATAGACGGCGTGGACATGCGGGCGGAG
  GTTGGGCTTCTGAGCCGGAACATCATAGTGATGGGGGAGATGGAGGACAAATGCTACCCCTACAGAAACC
  ACATCTGCAATTTCTTTGACTTCGATACCTTTGGGGGCCACATCAAGTTTGCTCTGGGATTTAAGGCAGC
  ACACTTGGAGGGCACGGAGCTGAAGCATATGGGACAGCAGCTGGTGGGTCAGTACCCGATTCACTTCCAC
  CTGGCCGGTGATGTAGACGAAAGGGGAGGTTATGACCCACCCACATACATCAGGGACCTCTCCATCCATC
  ATACATTCTCTCGCTGCGTCACAGTCCATGGCTCCAATGGCTTGTTGATCAAGGACGTTGTGGGCTATAA
  CTCTTTGGGCCACTGCTTCTTCACGGAAGATGGGCCGGAGGAACGCAACACTTTTGACCACTGTCTTGGC
  CTCCTTGTCAAGTCTGGAACCCTCCTCCCCTCGGACCGTGACAGCAAGATGTGCAAGATGATCACAGAGG
  ACTCCTACCCGGGGTACATCCCCAAGCCCAGGCAAGACTGCAATGCTGTGTCCACCTTCTGGATGGCCAA
  TCCCAACAACAACCTCATCAACTGTGCCGCTGCAGGATCTGAGGAAACTGGATTTTGGTTTATTTTTCAC
  CACGTACCAACGGGCCCCTCCGTGGGAATGTACTCCCCAGGTTATTCAGAGCACATTCCACTGGGAAAAT
  TCTATAACAACCGAGCACATTCCAACTACCGGGCTGGCATGATCATAGACAACGGAGTCAAAACCACCGA
  GGCCTCTGCCAAGGACAAGCGGCCGTTCCTCTCAATCATCTCTGCCAGATACAGCCCTCACCAGGACGCC
  GACCCGCTGAAGCCCCGGGAGCCGGCCATCATCAGACACTTCATTGCCTACAAGAACCAGGACCACGGGG
  CCTGGCTGCGCGGCGGGGATGTGTGGCTGGACAGCTGCCGGTTTGCTGACAATGGCATTGGCCTGACCCT
  GGCCAGTGGTGGAACCTTCCCGTATGACGACGGCTCCAAGCAAGAGATAAAGAACAGCTTGTTTGTTGGC
  GAGAGTGGCAACGTGGGGACGGAAATGATGGACAATAGGATCTGGGGCCCTGGCGGCTTGGACCATAGCG
  GAAGGACCCTCCCTATAGGCCAGAATTTTCCAATTAGAGGAATTCAGTTATATGATGGCCCCATCAACAT
  CCAAAACTGCACTTTCCGAAAGTTTGTGGCCCTGGAGGGCCGGCACACCAGCGCCCTGGCCTTCCGCCTG
  AATAATGCCTGGCAGAGCTGCCCCCATAACAACGTGACCGGCATTGCCTTTGAGGACGTTCCGATTACTT
  CCAGAGTGTTCTTCGGAGAGCCTGGGCCCTGGTTCAACCAGCTGGACATGGATGGGGATAAGACATCTGT
  GTTCCATGACGTCGACGGCTCCGTGTCCGAGTACCCTGGCTCCTACCTCACGAAGAATGACAACTGGCTG
  GTCCGGCACCCAGACTGCATCAATGTTCCCGACTGGAGAGGGGCCATTTGCAGTGGGTGCTATGCACAGA
  TGTACATTCAAGCCTACAAGACCAGTAACCTGCGAATGAAGATCATCAAGAATGACTTCCCCAGCCACCC
  TCTTTACCTGGAGGGGGCGCTCACCAGGAGCACCCATTACCAGCAATACCAACCGGTTGTCACCCTGCAG
  AAGGGCTACACCATCCACTGGGACCAGACGGCCCCCGCCGAACTCGCCATCTGGCTCATCAACTTCAACA
  AGGGCGACTGGATCCGAGTGGGGCTCTGCTACCCGCGAGGCACCACATTCTCCATCCTCTCGGATGTTCA
  CAATCGCCTGCTGAAGCAAACGTCCAAGACGGGCGTCTTCGTGAGGACCTTGCAGATGGACAAAGTGGAG
  CAGAGCTACCCTGGCAGGAGCCACTACTACTGGGACGAGGACTCAGGGCTGTTGTTCCTGAAGCTGAAAG
  CTCAGAACGAGAGAGAGAAGTTTGCTTTCTGCTCCATGAAAGGCTGTGAGAGGATAAAGATTAAAGCTCT
  GATTCCAAAGAACGCAGGCGTCAGTGACTGCACAGCCACAGCTTACCCCAAGTTCACCGAGAGGGCTGTC
  GTAGACGTGCCGATGCCCAAGAAGCTCTTTGGTTCTCAGCTGAAAACAAAGGACCATTTCTTGGAGGTGA
  AGATGGAGAGTTCCAAGCAGCACTTCTTCCACCTCTGGAACGACTTCGCTTACATTGAAGTGGATGGGAA
  GAAGTACCCCAGTTCGGAGGATGGCATCCAGGTGGTGGTGATTGACGGGAACCAAGGGCGCGTGGTGAGC
  CACACGAGCTTCAGGAACTCCATTCTGCAAGGCATACCATGGCAGCTTTTCAACTATGTGGCGACCATCC
  CTGACAATTCCATAGTGCTTATGGCATCAAAGGGAAGATACGTCTCCAGAGGCCCATGGACCAGAGTGCT
  GGAAAAGCTTGGGGCAGACAGGGGTCTCAAGTTGAAAGAGCAAATGGCATTCGTTGGCTTCAAAGGCAGC
  TTCCGGCCCATCTGGGTGACACTGGACACTGAGGATCACAAAGCCAAAATCTTCCAAGTTGTGCCCATCC
  CTGTGGTGAAGAAGAAGAAGTTG
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG215547 representing NM_018689
  Red=Cloning site Green=Tags(s)
  
  MGAAGRQDFLFKAMLTISWLTLTCFPGATSTVAAGCPDQSPELQPWNPGHDQDHHVHIGQGKTLLLTSSA
  TVYSIHISEGGKLVIKDHDEPIVLRTRHILIDNGGELHAGSALCPFQGNFTIILYGRADEGIQPDPYYGL
  KYIGVGKGGALELHGQKKLSWTFLNKTLHPGGMAEGGYFFERSWGHRGVIVHVIDPKSGTVIHSDRFDTY
  RSKKESERLVQYLNAVPDGRILSVAVNDEGSRNLDDMARKAMTKLGSKHFLHLGFRHPWSFLTVKGNPSS
  SVEDHIEYHGHRGSAAARVFKLFQTEHGEYFNVSLSSEWVQDVEWTEWFDHDKVSQTKGGEKISDLWKAH
  PGKICNRPIDIQATTMDGVNLSTEVVYKKGQDYRFACYDRGRACRSYRVRFLCGKPVRPKLTVTIDTNVN
  STILNLEDNVQSWKPGDTLVIASTDYSMYQAEEFQVLPCRSCAPNQVKVAGKPMYLHIGEEIDGVDMRAE
  VGLLSRNIIVMGEMEDKCYPYRNHICNFFDFDTFGGHIKFALGFKAAHLEGTELKHMGQQLVGQYPIHFH
  LAGDVDERGGYDPPTYIRDLSIHHTFSRCVTVHGSNGLLIKDVVGYNSLGHCFFTEDGPEERNTFDHCLG
  LLVKSGTLLPSDRDSKMCKMITEDSYPGYIPKPRQDCNAVSTFWMANPNNNLINCAAAGSEETGFWFIFH
  HVPTGPSVGMYSPGYSEHIPLGKFYNNRAHSNYRAGMIIDNGVKTTEASAKDKRPFLSIISARYSPHQDA
  DPLKPREPAIIRHFIAYKNQDHGAWLRGGDVWLDSCRFADNGIGLTLASGGTFPYDDGSKQEIKNSLFVG
  ESGNVGTEMMDNRIWGPGGLDHSGRTLPIGQNFPIRGIQLYDGPINIQNCTFRKFVALEGRHTSALAFRL
  NNAWQSCPHNNVTGIAFEDVPITSRVFFGEPGPWFNQLDMDGDKTSVFHDVDGSVSEYPGSYLTKNDNWL
  VRHPDCINVPDWRGAICSGCYAQMYIQAYKTSNLRMKIIKNDFPSHPLYLEGALTRSTHYQQYQPVVTLQ
  KGYTIHWDQTAPAELAIWLINFNKGDWIRVGLCYPRGTTFSILSDVHNRLLKQTSKTGVFVRTLQMDKVE
  QSYPGRSHYYWDEDSGLLFLKLKAQNEREKFAFCSMKGCERIKIKALIPKNAGVSDCTATAYPKFTERAV
  VDVPMPKKLFGSQLKTKDHFLEVKMESSKQHFFHLWNDFAYIEVDGKKYPSSEDGIQVVVIDGNQGRVVS
  HTSFRNSILQGIPWQLFNYVATIPDNSIVLMASKGRYVSRGPWTRVLEKLGADRGLKLKEQMAFVGFKGS
  FRPIWVTLDTEDHKAKIFQVVPIPVVKKKKL
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018689
• ORF Size: 4083 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_018689.3
• RefSeq Size: 7128 bp
• RefSeq ORF: 4086 bp
• Locus ID: 57214
• UniProt ID: Q8WUJ3
• Cytogenetics: 15q25.1
• Gene Summary: Mediates depolymerization of hyaluronic acid (HA) via the cell membrane-associated clathrin-coated pit endocytic pathway. Binds to hyaluronic acid. Hydrolyzes high molecular weight hyaluronic acid to produce an intermediate-sized product, a process that may occur through rapid vesicle endocytosis and recycling without intracytoplasmic accumulation or digestion in lysosomes. Involved in hyaluronan catabolism in the dermis of the skin and arthritic synovium. Positively regulates epithelial-mesenchymal transition (EMT), and hence tumor cell growth, invasion and cancer dissemination. In collaboration with HSPA5/BIP, promotes cancer cell migration in a calcium and PKC-dependent manner. May be involved in hearing.[UniProtKB/Swiss-Prot Function]