CILK1 (NM_014920) Human Untagged Clone

CAT#: SC304184

ICK (untagged)-Human intestinal cell (MAK-like) kinase (ICK), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CILK1
• Synonyms: ECO; EJM10; hICK; ICK; LCK2; MRK
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC304184 representing NM_014920.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGAATAGATACACAACAATCAGGCAGCTCGGGGATGGAACCTACGGTTCCGTCCTGCTGGGAAGAAGC
  ATTGAGTCTGGGGAGCTGATCGCTATTAAAAAAATGAAAAGAAAATTTTATTCCTGGGAGGAATGCATG
  AACCTTCGGGAGGTTAAGTCTTTAAAGAAGCTCAACCATGCCAATGTAGTCAAATTAAAAGAAGTTATC
  AGGGAAAATGATCATCTTTATTTTATCTTCGAGTACATGAAGGAAAATCTTTACCAGCTCATTAAAGAG
  AGAAATAAGTTGTTTCCTGAGTCTGCTATAAGGAATATCATGTATCAGATATTACAAGGACTCGCATTT
  ATTCACAAACACGGCTTCTTTCATCGAGACTTAAAGCCTGAGAACCTCCTCTGCATGGGACCAGAACTT
  GTGAAAATTGCAGACTTTGGTTTGGCCCGAGAAATACGATCAAAACCTCCATATACAGATTATGTATCT
  ACCAGATGGTACAGGGCTCCAGAAGTACTCCTGAGGTCTACCAACTACAGCTCCCCCATTGACGTCTGG
  GCGGTGGGCTGCATCATGGCAGAAGTTTACACCCTCAGGCCACTCTTCCCTGGAGCCAGTGAAATTGAC
  ACAATATTCAAAATTTGCCAAGTGCTGGGGACACCAAAAAAGACTGACTGGCCTGAAGGCTATCAACTT
  TCAAGTGCAATGAACTTCCGTTGGCCACAGTGTGTACCCAATAACTTAAAGACCTTGATTCCCAATGCT
  AGCAGTGAAGCAGTCCAGCTCCTGAGAGACATGCTTCAGTGGGATCCCAAGAAACGACCAACAGCTAGT
  CAGGCACTTCGATATCCTTACTTCCAAGTTGGACACCCACTAGGCAGCACCACACAAAACCTTCAGGAT
  TCAGAAAAACCACAGAAAGGCATCCTGGAAAAGGCAGGCCCACCTCCTTATATTAAGCCAGTCCCACCT
  GCCCAGCCACCAGCCAAGCCACACACACGAATTTCTTCACGACAGCATCAAGCCAGCCAGCCCCCTCTG
  CATCTCACGTACCCCTACAAAGCAGAGGTCTCCAGGACAGATCACCCAAGCCATCTCCAGGAGGACAAG
  CCAAGCCCGTTGCTTTTCCCATCCCTCCACAACAAGCATCCACAGTCGAAAATCACAGCTGGCCTGGAG
  CACAAAAATGGTGAGATAAAGCCAAAGAGTAGGAGAAGGTGGGGTCTTATTTCCAGGTCAACAAAGGAT
  TCAGATGATTGGGCTGACTTGGATGACTTGGATTTCAGTCCATCCCTCAGCAGGATTGACCTGAAAAAC
  AAGAAAAGACAGAGTGATGACACTCTCTGCAGGTTTGAGAGTGTTTTGGACCTGAAGCCCTCTGAGCCT
  GTGGGCACAGGAAACAGTGCCCCCACCCAGACGTCATATCAGCGGCGAGACACGCCCACCCTGAGATCT
  GCAGCCAAGCAGCACTATTTGAAGCACTCTCGATACTTGCCTGGGATCAGTATAAGAAATGGCATACTC
  TCGAATCCAGGCAAGGAATTTATTCCACCTAATCCATGGTCTAGTTCTGGCTTGTCTGGAAAATCTTCA
  GGGACAATGTCAGTAATCAGCAAAGTAAATTCAGTTGGTTCCAGCTCTACAAGTTCTAGTGGACTGACT
  GGAAACTATGTCCCTTCCTTTCTGAAAAAAGAAATCGGTTCTGCTATGCAGAGGGTACACCTAGCACCT
  ATTCCAGACCCTTCCCCTGGTTATTCCTCCCTGAAGGCCATGAGACCTCATCCTGGGCGACCATTCTTC
  CACACCCAGCCTAGAAGCACTCCTGGGTTGATACCACGGCCTCCAGCCGCCCAGCCAGTGCATGGCCGG
  ACAGACTGGGCTTCCAAGTACGCATCTCGGCGATGA
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_014920
• Insert Size: 1899 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014920.3
• RefSeq Size: 6127 bp
• RefSeq ORF: 1899 bp
• Locus ID: 22858
• UniProt ID: Q9UPZ9
• Cytogenetics: 6p12.1
• Protein Families: Druggable Genome, Protein Kinase
• MW: 71.4 kDa
• Gene Summary: Eukaryotic protein kinases are enzymes that belong to a very extensive family of proteins which share a conserved catalytic core common with both serine/threonine and tyrosine protein kinases. This gene encodes an intestinal serine/threonine kinase harboring a dual phosphorylation site found in mitogen-activating protein (MAP) kinases. The protein localizes to the intestinal crypt region and is thought to be important in intestinal epithelial cell proliferation and differentiation. Alternative splicing has been observed at this locus and two variants, encoding the same isoform, have been identified. [provided by RefSeq, Jul 2008]
  Transcript Variant: This variant (1) represents the shorter transcript. Variants 1 and 2 encode the same protein.