GJA3 (NM_021954) Human Untagged Clone

CAT#: SC123942

GJA3 (untagged)-Human gap junction protein, alpha 3, 46kDa (GJA3)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GJA3
• Synonyms: CTRCT14; CX46; CZP3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >SC123942 representing NM_021954.
  Blue=Insert sequence Red=Cloning site Green=Tag(s)
  
  GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG
  GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC
  ATGGGCGACTGGAGCTTTCTGGGAAGACTCTTAGAAAATGCACAGGAGCACTCCACGGTCATCGGCAAG
  GTTTGGCTGACCGTGCTGTTCATCTTCCGCATCTTGGTGCTGGGGGCCGCGGCGGAGGACGTGTGGGGC
  GATGAGCAGTCAGACTTCACCTGCAACACCCAGCAGCCGGGCTGCGAGAACGTCTGCTACGACAGGGCC
  TTCCCCATCTCCCACATCCGCTTCTGGGCGCTGCAGATCATCTTCGTGTCCACGCCCACCCTCATCTAC
  CTGGGCCACGTGCTGCACATCGTGCGCATGGAAGAGAAGAAGAAAGAGAGGGAGGAGGAGGAGCAGCTG
  AAGAGAGAGAGCCCCAGCCCCAAGGAGCCACCGCAGGACAATCCCTCGTCGCGGGACGACCGCGGCAGG
  GTGCGCATGGCCGGGGCGCTGCTGCGGACCTACGTCTTCAACATCATCTTCAAGACGCTGTTCGAGGTG
  GGCTTCATCGCCGGCCAGTACTTTCTGTACGGCTTCGAGCTGAAGCCGCTCTACCGCTGCGACCGCTGG
  CCCTGCCCCAACACGGTGGACTGCTTCATCTCCAGGCCCACGGAGAAGACCATCTTCATCATCTTCATG
  CTGGCGGTGGCCTGCGCGTCCCTGCTGCTCAACATGCTGGAGATCTACCACCTGGGCTGGAAGAAGCTC
  AAGCAGGGCGTGACCAGCCGCCTCGGCCCGGACGCCTCCGAGGCCCCGCTGGGGACAGCCGATCCCCCG
  CCCCTGCCCCCCAGCTCCCGGCCGCCCGCCGTTGCCATCGGGTTCCCACCCTACTATGCGCACACCGCT
  GCGCCCCTGGGACAGGCCCGCGCCGTGGGCTACCCCGGGGCCCCGCCACCAGCCGCGGACTTCAAAATG
  CTAGCCCTGACCGAGGCGCGCGGAAAGGGCCAGTCCGCCAAGCTCTACAACGGCCACCACCACCTGCTG
  ATGACTGAGCAGAACTGGGCCAACCAGGCGGCCGAGCGGCAGCCCCCGGCGCTCAAGGCTTACCCGGCA
  GCGTCCACGCCTGCAGCCCCCAGCCCCGTCGGCAGCAGCTCCCCGCCACTCGCGCACGAGGCTGAGGCG
  GGCGCGGCGCCCCTGCTGCTGGATGGGAGCGGCAGCAGTCTGGAGGGGAGCGCCCTGGCAGGGACCCCC
  GAGGAGGAGGAGCAGGCCGTGACCACCGCGGCCCAGATGCACCAGCCGCCCTTGCCCCTCGGAGACCCA
  GGTCGGGCCAGCAAGGCCAGCAGGGCCAGCAGCGGGCGGGCCAGACCGGAGGACTTGGCCATCTAG
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT
  TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC
  
• Restriction Sites: SgfI-MluI
• ACCN: NM_021954
• Insert Size: 1308 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021954.2
• RefSeq Size: 5219 bp
• RefSeq ORF: 1308 bp
• Locus ID: 2700
• UniProt ID: Q9Y6H8
• Cytogenetics: 13q12.11
• Protein Families: Druggable Genome, Ion Channels: Other, Transmembrane
• MW: 47.4 kDa
• Gene Summary: The protein encoded by this gene is a connexin and is a component of lens fiber gap junctions. Defects in this gene are a cause of zonular pulverulent cataract type 3 (CZP3). [provided by RefSeq, Jan 2010]