Methylmalonyl Coenzyme A mutase (MUT) (NM_000255) Human Untagged Clone

CAT#: SC120001

MUT (untagged)-Human methylmalonyl CoA mutase (MUT), nuclear gene encoding mitochondrial protein

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: Methylmalonyl Coenzyme A mutase
• Synonyms: MCM; MUT
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC120001 sequence for NM_000255 edited (data generated by NextGen Sequencing)
  ATGTTAAGAGCTAAGAATCAGCTTTTTTTACTTTCACCTCATTACCTGAGGCAGGTAAAA
  GAATCATCAGGCTCCAGGCTCATACAGCAACGACTTCTACACCAGCAACAGCCCCTTCAC
  CCAGAATGGGCTGCCCTGGCTAAAAAGCAGCTGAAAGGCAAAAACCCAGAAGACCTAATA
  TGGCACACCCCGGAAGGGATCTCTATAAAACCCTTGTATTCCAAGAGAGATACTATGGAC
  TTACCTGAAGAACTTCCAGGAGTGAAGCCATTCACACGTGGACCATATCCTACCATGTAT
  ACCTTTAGGCCCTGGACCATCCGCCAGTATGCTGGTTTTAGTACTGTGGAAGAAAGCAAT
  AAGTTCTATAAGGACAACATTAAGGCTGGTCAGCAGGGATTATCAGTTGCCTTTGATCTG
  GCGACACATCGTGGCTATGATTCAGACAACCCTCGAGTTCGTGGTGATGTTGGAATGGCT
  GGAGTTGCTATTGACACTGTGGAAGATACCAAAATTCTTTTTGATGGAATTCCTTTAGAA
  AAAATGTCAGTTTCCATGACTATGAATGGAGCAGTTATTCCAGTTCTTGCAAATTTTATA
  GTAACTGGAGAAGAACAAGGTGTACCTAAAGAGAAGCTTACTGGTACCATCCAAAATGAT
  ATACTAAAGGAATTTATGGTTCGAAATACATACATTTTTCCTCCAGAACCATCCATGAAA
  ATTATTGCTGACATATTTGAATATACAGCAAAGCACATGCCAAAATTTAATTCAATTTCA
  ATTAGTGGATACCATATGCAGGAAGCAGGGGCTGATGCCATTCTGGAGCTGGCCTATACT
  TTAGCAGATGGATTGGAGTACTCTAGAACTGGACTCCAGGCTGGCCTGACAATTGATGAA
  TTTGCACCAAGGTTGTCTTTCTTCTGGGGAATTGGAATGAATTTCTATATGGAAATAGCA
  AAGATGAGAGCTGGTAGAAGACTCTGGGCTCACTTAATAGAGAAAATGTTTCAGCCTAAA
  AACTCAAAATCTCTTCTTCTAAGAGCACACTGTCAGACATCTGGATGGTCACTTACTGAG
  CAGGATCCCTACAATAATATTGTCCGTACTGCAATAGAAGCAATGGCAGCAGTATTTGGA
  GGGACTCAGTCTTTGCACACAAATTCTTTTGATGAAGCTTTGGGTTTGCCAACTGTGAAA
  AGTGCTCGAATTGCCAGGAACACACAAATCATCATTCAAGAAGAATCTGGGATTCCCAAA
  GTGGCTGATCCTTGGGGAGGTTCTTACATGATGGAATGTCTCACAAATGATGTTTATGAT
  GCTGCTTTAAAGCTCATTAATGAAATTGAAGAAATGGGTGGAATGGCCAAAGCTGTAGCT
  GAGGGAATACCTAAACTTCGAATTGAAGAATGTGCTGCCCGAAGACAAGCTAGAATAGAT
  TCTGGTTCTGAAGTAATTGTTGGAGTAAATAAGTACCAGTTGGAAAAAGAAGACGCTGTA
  GAAGTTCTGGCAATTGATAATACTTCAGTGCGAAACAGGCAGATTGAAAAACTTAAGAAG
  ATCAAATCCAGCAGGGATCAAGCTTTGGCTGAACGTTGTCTTGCTGCACTAACCGAATGT
  GCTGCTAGCGGAGATGGAAATATCCTGGCTCTTGCAGTGGATGCATCTCGGGCAAGATGT
  ACAGTGGGAGAAATCACAGATGCCCTGAAAAAGGTATTTGGTGAACATAAAGCGAATGAT
  CGAATGGTGAGTGGAGCATATCGCCAGGAATTTGGAGAAAGTAAAGAGATAACATCTGCT
  ATCAAGAGGGTTCATAAATTCATGGAACGTGAAGGTCGCAGACCTCGTCTTCTTGTAGCA
  AAAATGGGACAAGATGGCCATGACAGAGGAGCAAAAGTTATTGCTACAGGATTTGCTGAT
  CTTGGTTTTGATGTGGACATAGGCCCTCTTTTCCAGACTCCTCGTGAAGTGGCCCAGCAG
  GCTGTGGATGCGGATGTGCATGCTGTGGGCATAAGCACCCTCGCTGCTGGTCATAAAACC
  CTAGTTCCTGAACTCATCAAAGAACTTAACTCCCTTGGACGGCCAGATATTCTTGTCATG
  TGTGGAGGGGTGATACCACCTCAGGATTATGAATTTCTGTTTGAAGTTGGTGTTTCCAAT
  GTATTTGGTCCTGGGACTCGAATTCCAAAGGCTGCCGTTCAGGTGCTTGATGATATTGAG
  AAGTGTTTGGAAAAGAAGCAGCAATCTGTATAA
  
  Clone variation with respect to NM_000255.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_000255 unedited
  TGAAACACGACTTACTATAGGGCGGCCGCGAATTCGGCACGAGGCAGAGTCGTCCGGCTG
  ACGCTGGCGGTGGCGCGGTTTGTGTGGGCTTGGTGAGGGCGGGGAGGCCTGGCTGTGTGG
  ATGTCTGACAGGTGAGGCGGGGGACGCAGAAGTGCAGCCGCCCTCTCCCACAGCGGAGTC
  CAAAACAGGCCTACCAGTCAGTTCTTATTTCTATTGGGTGTTTCCATGCTCCACCATGTT
  AAGAGCTAAGAATCAGCTTTTTTTACTTTCACCTCATTACCTGAGGCAGGTAAAAGAATC
  ATCAGGCTCCAGGCTCATACAGCAACGACTTCTACACCAGCAACAGCCCCTTCACCCAGA
  ATGGGCTGCCCTGGCTAAAAAGCAGCTGAAAGGCAAAAACCCAGAAGACCTAATATGGCA
  CACCCCGGAAGGGATCTCTATAAAACCCTTGTATTCCAAGAGAGATACTATGGACTTACC
  TGAAGAACTTCCAGGAGTGAAGCCATTCACACGTGGACCATATCCTACCATGTATACCTT
  TAGGCCCTGGACCATCCGCCAGTATGCTGGTTTTAGTACTGTGGAAGAAAGCAATAAGTT
  CTATAAGGACAACATTAAGGCTGGTCAGCAGGGATTATCAGTTGCCTTTGATCTGGCGAC
  ACATCGTGGCTATGATTCAGACAACCCTCGAGTTCGTGGTGATGTTGGAATGGCTGGAGT
  TGCTATTGACACTGTGGAAGATACCAAAATTCTTTTTGATGGAATTCCTTTANAAAAAAT
  GTCAGTTTCCATGACTATGAAATGGAGCAGTATTCCAGTTCTTGCAAATTNTATAGTAAC
  TGGAGAAGAACAAGGTGTACCTAAAGAGAGCTTACTGGTACCATNCANATGAATACTNAA
  GGATT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_000255 unedited
  TGACCGCGGCCGCAATCTAGAGTCGAGTTTTTTTTTTTTTTTTTTGAAGTGAAACTGTAT
  GTACATACTTATAGCATGACACCAGGCCTATAAGTAAGGTATTTTAAAGTAAAGCTTTCA
  AGGAAAGTACAAATCAGGTATTGAAATTAAATTGAAGACATAGCTTTACTCTCTTCTTTG
  ATCATAACTAAAATAATATTTTAGACAAAAGCTAAAACAAAAAGAGGATATTATACAGAT
  TGCTGCTTCTTTTCCAAACACTTCTCAATATCATCAAGCACCTGAACGGCAGCCTTTGGA
  ATTCGAGTCCCAGGACCAAATACATTGGAAACACCAACTTCAAACAGAAATTCATAATCC
  TGAGGTGGTATCACCCCTCCACACATGACAAGAATATCTGGCCGTCCAAGGGAGTTAAGT
  TCTTTGATGAGTTCAGGAACTAGGGTTTTATGACCAGCAGCGAGGGTGCTTATGCCCACA
  GCATGCACATCCGCATCCACAGCCTGCTGGGCCACTTCACGAGGAGTCTGGAAAAGAGGG
  CCTATGTCCACATCAAAACCAAGATCAGCAAATCCTGTAGCAATAACTTTTGCTCCTCTG
  TCATGGCCATCTTGTCCCATTTTTGCTACAAGAAGACGAGGTCTGCGACCTTCACGTTCC
  ATGAATTTATGAACCCTCTTGATAGCAGATGTTATCTCTTTACTTTCTCCAAATTCCTGG
  CGATATGCTCCACTCACCATTCGATCATTCGCTTTATGTTCACCAAATACCTTTTTCAGG
  GCATCTGTGATTTCTCCACTGTACATCTTGCCCGAGATGCATCCACTTGCAGAGCCAAGA
  TTTTCCATCTCCGCTACCACCACATCCCGTTATTGCAGCAAGACAACGTTCAGCCAAGCT
  TGATCCCTGCTGAAT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_000255
• Insert Size: 2710 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000255.1, NP_000246.1
• RefSeq Size: 2798 bp
• RefSeq ORF: 2253 bp
• Locus ID: 4594
• UniProt ID: P22033
• Cytogenetics: 6p12.3
• Domains: MM_CoA_mutase, B12-binding
• Protein Families: Druggable Genome
• Protein Pathways: Metabolic pathways, Propanoate metabolism, Valine, leucine and isoleucine degradation
• Gene Summary: This gene encodes the mitochondrial enzyme methylmalonyl Coenzyme A mutase. In humans, the product of this gene is a vitamin B12-dependent enzyme which catalyzes the isomerization of methylmalonyl-CoA to succinyl-CoA, while in other species this enzyme may have different functions. Mutations in this gene may lead to various types of methylmalonic aciduria. [provided by RefSeq, Jul 2008]