GTF3C2 (NM_001521) Human Untagged Clone

CAT#: SC119183

GTF3C2 (untagged)-Human general transcription factor IIIC, polypeptide 2, beta 110kDa (GTF3C2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GTF3C2
• Synonyms: TFIIIC-BETA; TFIIIC110
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_001521, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGATACCTGCGGGGTCGGCTATGTTGCCCTGGGGGAGGCCGGCCCCGTGGGGAACATGACTGTGGTAG
  ACTCTCCTGGACAAGAGGTGCTAAATCAGCTTGATGTCAAGACCTCTTCAGAAATGACCAGTGCAGAGGC
  TTCCGTAGAGATGTCATTACCTACCCCTTTGCCTGGATTTGAGGATTCTCCTGATCAGAGGAGGCTCCCT
  CCAGAGCAGGAAAGCCTCTCCAGACTGGAACAGCCAGATCTTTCTTCAGAGATGTCAAAGGTCTCAAAGC
  CTAGGGCCTCAAAGCCTGGCCGGAAGAGAGGTGGTAGGACACGAAAAGGCCCCAAAAGGCCCCAACAGCC
  TAATCCTCCATCAGCCCCACTGGTTCCTGGTCTCTTAGATCAATCCAACCCTCTGTCCACCCCCATGCCT
  AAGAAACGAGGTCGAAAGTCCAAGGCAGAGCTGCTGCTGCTGAAGTTGTCAAAAGACCTAGATCGGCCAG
  AATCTCAATCTCCAAAGAGGCCCCCTGAGGACTTTGAGACCCCTTCTGGGGAACGACCCCGCCGAAGGGC
  TGCCCAAGTGGCACTTCTGTATCTTCAGGAACTGGCTGAAGAGCTCTCAACAGCCCTGCCTGCCCCTGTG
  TCCTGTCCTGAGGGCCCCAAGGTGAGCAGCCCCACCAAACCGAAGAAGATCCGGCAGCCAGCAGCCTGTC
  CAGGTGGAGAAGAGGTGGATGGTGCTCCACGGGATGAAGACTTTTTTCTCCAGGTTGAGGCTGAAGATGT
  GGAAGAAAGTGAGGGCCCAAGTGAGAGCTCATCTGAACCTGAGCCTGTAGTGCCCCGAAGCACCCCACGA
  GGATCTACTTCAGGGAAACAGAAACCACACTGCCGAGGAATGGCTCCCAATGGCTTACCAAATCATATCA
  TGGCTCCTGTTTGGAAGTGCCTCCATCTCACCAAGGACTTCCGAGAGCAGAAACATTCATACTGGGAGTT
  TGCTGAGTGGATTCCTTTAGCCTGGAAGTGGCACTTGTTATCTGAGCTTGAGGCCGCTCCCTACCTGCCC
  CAGGAGGAGAAGTCTCCATTGTTTTCTGTACAACGTGAAGGGCTACCTGAAGATGGCACCCTCTACCGAA
  TAAACAGATTTAGCTCGATCACAGCACATCCAGAGCGCTGGGATGTGTCCTTCTTCACGGGGGGACCGCT
  CTGGGCTCTGGACTGGTGCCCAGTGCCAGAGGGGGCAGGAGCCTCGCAATATGTGGCTCTTTTCTCCAGC
  CCTGACATGAATGAGACACACCCACTGAGCCAGCTTCATTCGGGTCCTGGGCTGCTCCAGCTCTGGGGCC
  TTGGGACCTTGCAGCAAGAAAGCTGTCCTGGCAACAGGGCCCACTTTGTCTATGGGATTGCTTGTGACAA
  CGGCTGCATCTGGGACCTCAAGTTCTGCCCCAGTGGAGCATGGGAACTTCCAGGCACCCCTCGGAAGGCT
  CCTCTCCTGCCCCGGTTGGGTCTCTTGGCTCTGGCCTGCTCAGACGGGAAAGTACTGCTATTCAGTCTAC
  CCCATCCGGAGGCCCTGCTGGCTCAGCAACCCCCAGATGCAGTGAAGCCTGCCATATATAAGGTACAATG
  TGTGGCAACTCTGCAGGTGGGGTCTATGCAAGCTACAGACCCCTCTGAGTGTGGTCAGTGCCTTAGCCTG
  GCCTGGATGCCTACCAGGCCCCACCAACACCTAGCTGCTGGATATTATAATGGCATGGTGGTTTTCTGGA
  ACCTTCCCACTAACTCACCCCTGCAGCGGATACGGCTCTCTGATGGCTCCTTAAAGCTCTACCCCTTCCA
  GTGTTTCCTAGCCCATGACCAGGCTGTGCGTACCCTTCAATGGTGCAAAGCTAACAGCCATTTCCTTGTC
  TCTGCGGGGAGTGACCGGAAAATCAAATTCTGGGACCTTCGACGTCCTTACGAACCCATAAACTCTATCA
  AGCGCTTCTTGAGTACAGAACTGGCCTGGCTGCTTCCCTACAATGGTGTCACTGTGGCTCAGGACAACTG
  CTATGCCTCTTATGGACTCTGTGGGATTCATTATATTGACGCTGGTTACCTTGGTTTCAAGGCCTACTTC
  ACTGCTCCTCGAAAAGGCACCGTTTGGAGTCTTTCAGGATCCGACTGGCTTGGGACAATAGCTGCAGGAG
  ATATATCCGGGGAGCTCATTGCTGCTATATTACCAGATATGGCACTGAATCCAATAAATGTCAAGCGACC
  TGTAGAGCGAAGATTTCCTATATATAAAGCAGATCTGATACCGTATCAGGACAGTCCTGAAGGTCCAGAC
  CATTCTTCTGCTTCATCTGGGGTCCCCAACCCTCCTAAGGCTCGAACTTACACTGAAACTGTCAACCATC
  ACTACTTGCTCTTTCAAGACACAGATTTGGGTTCATTCCATGATCTGCTCCGTAGAGAACCAATGCTGCG
  CATGCAGGAGGGAGAGGGGCATTCTCAACTCTGCCTGGACAGGCTGCAGCTGGAGGCTATTCATAAGGTA
  CGTTTCAGCCCAAACCTGGACTCCTATGGATGGCTGGTATCTGGGGGGCAGTCAGGGCTGGTTCGAATCC
  ATTTTGTCCGTGGACTCGCCTCCCCACTGGGCCACCGTATGCAGCTTGAAAGCCGAGCCCACTTCAATGC
  TATGTTCCAACCATCCTCCCCCACTAGACGGCCTGGCTTCTCTCCAACCAGCCATCGCCTTCTGCCCACT
  CCCTAG
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001521 unedited
  AATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCTCGTGCCGAATTCGGCA
  CGAGGCAGAATCCCCTGAAGAGCTTGTTAAAACACAAGGTGCTGGCCCCATCCAGAATTG
  CTGATTCGGGGCAGCCATTTTGGGGGGTGCTGATGGATACCTGCGGGGTCGGCTATGTTG
  CCCTGGGGGAGGCCGGCCCCGTGGGGAACATGACTGTGGTAGACTCTCCTGGACAAGAGG
  TGCTAAATCAGCTTGATGTCAAGACCTCTTCAGAAATGACCAGTGCAGAGGCTTCCGTAG
  AGATGTCATTACCTACCCCTTTGCCTGGATTTGAGGATTCTCCTGATCAGAGGAGGCTCC
  CTCCAGAGCAGGAAAGCCTCTCCAGACTGGAACAGCCAGATCTTTCTTCAGAGATGTCAA
  AGGTCTCAAAGCCTAGGGCCTCAAAGCCTGGCCGGAAGAGAGGTGGTAGGACACGAAAAG
  GCCCCAAAAGGCCCCAACAGCCTAATCCTCCATCAGCCCCACTGGTTCCTGGTCTCTTAG
  ATCAATCCAACCCTCTGTCCACCCCCATGCCTAAGAAACGAGGTCGAAAGTCCAAGGCAG
  AGCTGCTGCTGCTGAAGTTGTCAAAAGACCTAGATCGGCCAGAATCTCAATCTCCAAAGA
  GGCCCCCCTGAGACTNTGAGACCCNCTTCTGGGAACGANCCCCGCCGAAGGCTGCCCAAG
  TGGCACTTCTGTATCTTCAGGAACTGGCTGAAGAGCTCTCAACAGCCCTGCCTGCCCCTG
  TGTCCTGTCCTGAGGNNCCCCANGTGAGCAGCCCACCANACCGAGNAAGANCCGCAGCCA
  GCAGCCTGTCCCAGTGGAGAAAGAGTGGGATGGNTGCTCACAGGATGAAGACTTTNTTCT
  NCAGNTGAGGCTGAAGAGTGNAAAAAATGAGGCCCNAGTGAGACTCTCTGACT
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001521 unedited
  CGGCCGCATTCTAGNATCGAGTTTTTTTTTTTTTTTTTTTTTTTTTTTTGAAGTAACTGA
  AGATATTTAATCTAGAGAAGATTTGGGAAACACATGATAGCTATGGTTAAATACTTAACA
  GGGCAATCACAGGGAAGATGACTAGATTTCCTAACATCCATGAGTGAAATTTATAGAAGT
  ATACTCTCTGACTTGATATAAAGGAAGATTTTAAAAAACATGACTGTTCAGGAGTGTTCA
  AGTAGGGTCAGATGACCAGTGATTGGGAATACTTCGTAAGCAGGAGCAAGTAAGATCTGA
  GCCACTGTTCTATCGGTAGGGGGTCTGTGGTATTCCTTGGTCAAAGAAGTACTCTAAGCA
  ACTTCAGTCTCACGAATTACTATCACCCTCGTGGGCATACATGATGGTTACCCTAAAGAG
  GAAGTTTCAGAAGGCAGTAATATTGGATCCTGGAATAGTCAGACAGGAGCCTTCATGCAG
  ATACCCTTTTCAGTTCTCCATACACCCATTCACAAGTGGTCACAAAAACACCCAGTACCT
  TTACTTGGCTTTACCCACTTAACAATATGCTCAATATGAGCAGTTTGACTCCCAAGCCTA
  GGTCAGGCCTGAGTGTTTCTCATGAGCCATAGTCTTAGGCTGAGGAGCCTTGAGAGTTGG
  TGGAGGGAGAGCCCCCCTGCCCGCAGGGGGCTGTGATCACAGAAGTCTGTAGAAAGAGTG
  GGATCTAAGGAGTCCTGGGGAGGCAGGCCTAAAGGTCCAGGCCTGGCATGATCACTGTCC
  AGGGAAGGCCCCCAGNTTCTATGGCTCTGTGCATAGGGGCCACTTTGNTCTTGACCGACT
  TCACTTCAAGGATCTGGTGTGGGCCCAGGCTAGGNAGTGGGCANAAAGCGATGGGCTGGN
  TGAGAAANCCAGCCGTCTACTGGGGAGGATGTTGGNACTACCATGAAGTGGCTCGCCTTC
  AGCTGATACG
  
• Restriction Sites: ECoRI-NOT
• ACCN: NM_001521
• Insert Size: 3600 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001521.1, NP_001512.1
• RefSeq Size: 3976 bp
• RefSeq ORF: 2736 bp
• Locus ID: 2976
• UniProt ID: Q8WUA4
• Cytogenetics: 2p23.3
• Domains: WD40
• Protein Families: Transcription Factors
• Gene Summary: Required for RNA polymerase III-mediated transcription. Component of TFIIIC that initiates transcription complex assembly on tRNA and is required for transcription of 5S rRNA and other stable nuclear and cytoplasmic RNAs. May play a direct role in stabilizing interactions of TFIIIC2 with TFIIIC1.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) differs in the 5' UTR and coding sequence compared to variant 3. The resulting isoform (b) is shorter at the N-terminus compared to isoform a. Variants 1 and 2 both encode the same isoform (b).