GTF3C2 (NM_001521) Human Untagged Clone

CAT#: SC323740

GTF3C2 (untagged)-Human general transcription factor IIIC, polypeptide 2, beta 110kDa (GTF3C2), transcript variant 1

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: GTF3C2
• Synonyms: TFIIIC-BETA; TFIIIC110
• Vector: pCMV6-AC
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >OriGene sequence for NM_001521.2 CGAGGGGGCAGCCATTTTGGGGGGTGCTGATGGATACCTGCGGGGTCGGCTATGTTGCCC
  TGGGGGAGGCCGGCCCCGTGGGGAACATGACTGTGGTAGACTCTCCTGGACAAGAGGTGC
  TAAATCAGCTTGATGTCAAGACCTCTTCAGAAATGACCAGTGCAGAGGCTTCCGTAGAGA
  TGTCATTACCTACCCCTTTGCCTGGATTTGAGGATTCTCCTGATCAGAGGAGGCTCCCTC
  CAGAGCAGGAAAGCCTCTCCAGACTGGAACAGCCAGATCTTTCTTCAGAGATGTCAAAGG
  TCTCAAAGCCTAGGGCCTCAAAGCCTGGCCGGAAGAGAGGTGGTAGGACACGAAAAGGCC
  CCAAAAGGCCCCAACAGCCTAATCCTCCATCAGCCCCACTGGTTCCTGGTCTCTTAGATC
  AATCCAACCCTCTGTCCACCCCCATGCCTAAGAAACGAGGTCGAAAGTCCAAGGCAGAGC
  TGCTGCTGCTGAAGTTGTCAAAAGACCTAGATCGGCCAGAATCTCAATCTCCAAAGAGGC
  CCCCTGAGGACTTTGAGACCCCTTCTGGGGAACGACCCCGCCGAAGGGCTGCCCAAGTGG
  CACTTCTGTATCTTCAGGAACTGGCTGAAGAGCTCTCAACAGCCCTGCCTGCCCCTGTGT
  CCTGTCCTGAGGGCCCCAAGGTGAGCAGCCCCACCAAACCGAAGAAGATCCGGCAGCCAG
  CAGCCTGTCCAGGTGGAGAAGAGGTGGATGGTGCTCCACGGGATGAAGACTTTTTTCTCC
  AGGTTGAGGCTGAAGATGTGGAAGAAAGTGAGGGCCCAAGTGAGAGCTCATCTGAACCTG
  AGCCTGTAGTGCCCCGAAGCACCCCACGAGGATCTACTTCAGGGAAACAGAAACCACACT
  GCCGAGGAATGGCTCCCAATGGCTTACCAGATCATATCATGGCTCCTGTTTGGAAGTGCC
  TCCATCTCACCAAGGACTTCCGAGAGCAGAAACATTCATACTGGGAGTTTGCTGAGTGGA
  TTCCTTTAGCCTGGAAGTGGCACTTGTTATCTGAGCTTGAGGCCGCTCCCTACCTGCCCC
  AGGAGGAGAAGTCTCCATTGTTTTCTGTACAACGTGAAGGGCTACCTGAAGATGGCACCC
  TCTACCGAATAAACAGATTTAGCTCGATCACAGCACATCCAGAGCGCTGGGATGTGTCCT
  TCTTCACGGGGGGACCGCTCTGGGCTCTGGACTGGTGCCCAGTGCCAGAGGGGGCAGGAG
  CCTCGCAATATGTGGCTCTTTTCTCCAGCCCTGACATGAATGAGACACACCCACTGAGCC
  AGCTTCATTCGGGTCCTGGGCTGCTCCAGCTCTGGGGCCTTGGGACCTTGCAGCAAGAAA
  GCTGTCCTGGCAACAGGGCCCACTTTGTCTATGGGATTGCTTGTGACAACGGCTGCATCT
  GGGACCTCAAGTTCTGCCCCAGTGGAGCATGGGAACTTCCAGGCACCCCTCGGAAGGCTC
  CTCTCCTGCCCCGGTTGGGTCTCTTGGCTCTGGCCTGCTCAGACGGGAAAGTACTGCTAT
  TCAGTCTACCCCATCCGGAGGCCCTGCTGGCTCAGCAACCCCCAGATGCAGTGAAGCCTG
  CCATATATAAGGTACAATGTGTGGCAACTCTGCAGGTGGGGTCTATGCAAGCTACAGACC
  CCTCTGAGTGTGGTCAGTGCCTTAGCCTGGCCTGGATGCCTACCAGGCCCCACCAACACC
  TAGCTGCTGGATATTATAATGGCATGGTGGTTTTCTGGAACCTTCCCACTAACTCACCCC
  TGCAGCGGATACGGCTCTCTGATGGCTCCTTAAAGCTCTACCCCTTCCAGTGTTTCCTAG
  CCCATGACCAGGCTGTGCGTACCCTTCAATGGTGCAAAGCTAACAGCCATTTCCTTGTCT
  CTGCGGGGAGTGACCGGAAAATCAAATTCTGGGACCTTCGACGTCCTTACGAACCCATAA
  ACTCTATCAAGCGCTTCTTGAGTACAGAACTGGCCTGGCTGCTTCCCTACAATGGTGTCA
  CTGTGGCTCAGGACAACTGCTATGCCTCTTATGGACTCTGTGGGATTCATTATATTGACG
  CTGGTTACCTTGGTTTCAAGGCCTACTTCACTGCTCCTCGAAAAGGCACCGTTTGGAGTC
  TTTCAGGATCCGACTGGCTTGGGACAATAGCTGCAGGAGATATATCCGGGGAGCTCATTG
  CTGCTATATTACCAGATATGGCACTGAATCCAATAAATGTCAAGCGACCTGTAGAGCGAA
  GATTTCCTATATATAAAGCAGATCTGATACCGTATCAGGACAGTCCTGAAGGTCCAGACC
  ATTCTTCTGCTTCATCTGGGGTCCCCAACCCTCCTAAGGCTCGAACTTACACTGAAACTG
  TCAACCATCACTACTTGCTCTTTCAAGACACAGATTTGGGTTCATTCCATGATCTGCTCC
  GTAGAGAACCAATGCTGCGCATGCAGGAGGGAGAGGGGCATTCTCAACTCTGCCTGGACA
  GGCTGCAGCTGGAGGCTATTCATAAGGTACGTTTCAGCCCAAACCTGGACTCCTATGGAT
  GGCTGGTATCTGGGGGGCAGTCAGGGCTGGTTCGAATCCATTTTGTCCGTGGACTCGCCT
  CCCCACTGGGCCACCGTATGCAGCTTGAAAGCCGAGCCCACTTCAATGCTATGTTCCAAC
  CATCCTCCCCCACTAGACGGCCTGGCTTCTCTCCAACCAGCCATCGCCTTCTGCCCACTC
  CCTAGCCTTGGCCCACACCAGATCCTTGGAGTGAAGTCGGTCAAGAACAAATGGCCCCTA
  TGCACAGAGCCATAGGAACTGGGGGCCTTCCCTGGACAGTGATCATGCCAGGCCTGGACC
  TTTAGGCCTGCCTCCCCAGGACTCCTTAGATCCCACTCTTTCTACAGACTTCTGTGATCA
  CAGCCCCCTGCGGGCAGGGGGGCTCTCCCTCCACCAACTCTCAAGGCTCCTCAGCCTAAG
  ACTATGGCTCATGAGAAACACTCAGGCCTGACCTAGGCTTGGGAGTCAAACTGCTCATAT
  TGAGCATATTGTTAAGTGGGTAAAGCCAAGTAAAGGTACTGGGTGTTTTTGTGACCACTT
  GTGAATGGGTGTATGGAGAACTGAAAAGGGTATCTGCATGAAGGCTCCTGTCTGACTATT
  CCAGGATCCAATATTACTGCCTTCTGAAACTTCCTCTTTAGGGTAACCATCATGTATGCC
  CACGAGGGTGATAGTAATTCGTGAGACTGAAGTTGCTTAGAGTACTTCTTTGACCAAGGA
  ATACCACAGACACCCTACCGATAGAACAGTGGCTCAGATCTTACTTGCTCCTGCTTACGA
  AGTATTCCCAATCACTGGTCATCTGACCCTACTTGAACACTCCTGAACAGTCATGTTTTT
  TAAAATCTTCCTTTATATCAAGTCAGAGAGTATACTTCTATAAATTTCACTCATGGATGT
  TAGGAAATCTAGTCATCTTCCCTGTGATTGCCCTGTTAAGTATTTAACCATAGCTATCAT
  GTGTTTCCCAAATCTTCTCTAGATTAAATATCTTCAGTTACTTCAAAAAAAAAAAAAAAA
• Restriction Sites: ECoRI-NOT
• ACCN: NM_001521
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: This TrueClone is provided through our Custom Cloning Process that includes sub-cloning into OriGene's pCMV6 vector and full sequencing to provide a non-variant match to the expected reference without frameshifts, and is delivered as lyophilized plasmid DNA.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001521.2, NP_001512.1
• RefSeq Size: 3935 bp
• RefSeq ORF: 2736 bp
• Locus ID: 2976
• UniProt ID: Q8WUA4
• Cytogenetics: 2p23.3
• Domains: WD40
• Protein Families: Transcription Factors
• Gene Summary: Required for RNA polymerase III-mediated transcription. Component of TFIIIC that initiates transcription complex assembly on tRNA and is required for transcription of 5S rRNA and other stable nuclear and cytoplasmic RNAs. May play a direct role in stabilizing interactions of TFIIIC2 with TFIIIC1.[UniProtKB/Swiss-Prot Function]
  Transcript Variant: This variant (1) differs in the 5' UTR and coding sequence compared to variant 3. The resulting isoform (b) is shorter at the N-terminus compared to isoform a. Variants 1 and 2 both encode the same isoform (b).