GTF3C2 (NM_001521) Human Tagged ORF Clone

CAT#: RC202276

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GTF3C2 (Myc-DDK-tagged)-Human general transcription factor IIIC, polypeptide 2, beta 110kDa (GTF3C2), transcript variant 1



  "NM_001521" in other vectors (7)

Reconstitution Protocol

Special Offer: 20% off the control vector (empty cloning vector without insert). Use code "Shuttle20".

USD 477.00


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In Stock

Size
    • 10 ug


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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol GTF3C2
Synonyms TFIIIC-BETA; TFIIIC110
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC202276 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGATACCTGCGGGGTCGGCTATGTTGCCCTGGGGGAGGCCGGCCCCGTGGGGAACATGACTGTGGTAG
ACTCTCCTGGACAAGAGGTGCTAAATCAGCTTGATGTCAAGACCTCTTCAGAAATGACCAGTGCAGAGGC
TTCCGTAGAGATGTCATTACCTACCCCTTTGCCTGGATTTGAGGATTCTCCTGATCAGAGGAGGCTCCCT
CCAGAGCAGGAAAGCCTCTCCAGACTGGAACAGCCAGATCTTTCTTCAGAGATGTCAAAGGTCTCAAAGC
CTAGGGCCTCAAAGCCTGGCCGGAAGAGAGGTGGTAGGACACGAAAAGGCCCCAAAAGGCCCCAACAGCC
TAATCCTCCATCAGCCCCACTGGTTCCTGGTCTCTTAGATCAATCCAACCCTCTGTCCACCCCCATGCCT
AAGAAACGAGGTCGAAAGTCCAAGGCAGAGCTGCTGCTGCTGAAGTTGTCAAAAGACCTAGATCGGCCAG
AATCTCAATCTCCAAAGAGGCCCCCTGAGGACTTTGAGACCCCTTCTGGGGAACGACCCCGCCGAAGGGC
TGCCCAAGTGGCACTTCTGTATCTTCAGGAACTGGCTGAAGAGCTCTCAACAGCCCTGCCTGCCCCTGTG
TCCTGTCCTGAGGGCCCCAAGGTGAGCAGCCCCACCAAACCGAAGAAGATCCGGCAGCCAGCAGCCTGTC
CAGGTGGAGAAGAGGTGGATGGTGCTCCACGGGATGAAGACTTTTTTCTCCAGGTTGAGGCTGAAGATGT
GGAAGAAAGTGAGGGCCCAAGTGAGAGCTCATCTGAACCTGAGCCTGTAGTGCCCCGAAGCACCCCACGA
GGATCTACTTCAGGGAAACAGAAACCACACTGCCGAGGAATGGCTCCCAATGGCTTACCAGATCATATCA
TGGCTCCTGTTTGGAAGTGCCTCCATCTCACCAAGGACTTCCGAGAGCAGAAACATTCATACTGGGAGTT
TGCTGAGTGGATTCCTTTAGCCTGGAAGTGGCACTTGTTATCTGAGCTTGAGGCCGCTCCCTACCTGCCC
CAGGAGGAGAAGTCTCCATTGTTTTCTGTACAACGTGAAGGGCTACCTGAAGATGGCACCCTCTACCGAA
TAAACAGATTTAGCTCGATCACAGCACATCCAGAGCGCTGGGATGTGTCCTTCTTCACGGGGGGACCGCT
CTGGGCTCTGGACTGGTGCCCAGTGCCAGAGGGGGCAGGAGCCTCGCAATATGTGGCTCTTTTCTCCAGC
CCTGACATGAATGAGACACACCCACTGAGCCAGCTTCATTCGGGTCCTGGGCTGCTCCAGCTCTGGGGCC
TTGGGACCTTGCAGCAAGAAAGCTGTCCTGGCAACAGGGCCCACTTTGTCTATGGGATTGCTTGTGACAA
CGGCTGCATCTGGGACCTCAAGTTCTGCCCCAGTGGAGCATGGGAACTTCCAGGCACCCCTCGGAAGGCT
CCTCTCCTGCCCCGGTTGGGTCTCTTGGCTCTGGCCTGCTCAGACGGGAAAGTACTGCTATTCAGTCTAC
CCCATCCGGAGGCCCTGCTGGCTCAGCAACCCCCAGATGCAGTGAAGCCTGCCATATATAAGGTACAATG
TGTGGCAACTCTGCAGGTGGGGTCTATGCAAGCTACAGACCCCTCTGAGTGTGGTCAGTGCCTTAGCCTG
GCCTGGATGCCTACCAGGCCCCACCAACACCTAGCTGCTGGATATTATAATGGCATGGTGGTTTTCTGGA
ACCTTCCCACTAACTCACCCCTGCAGCGGATACGGCTCTCTGATGGCTCCTTAAAGCTCTACCCCTTCCA
GTGTTTCCTAGCCCATGACCAGGCTGTGCGTACCCTTCAATGGTGCAAAGCTAACAGCCATTTCCTTGTC
TCTGCGGGGAGTGACCGGAAAATCAAATTCTGGGACCTTCGACGTCCTTACGAACCCATAAACTCTATCA
AGCGCTTCTTGAGTACAGAACTGGCCTGGCTGCTTCCCTACAATGGTGTCACTGTGGCTCAGGACAACTG
CTATGCCTCTTATGGACTCTGTGGGATTCATTATATTGACGCTGGTTACCTTGGTTTCAAGGCCTACTTC
ACTGCTCCTCGAAAAGGCACCGTTTGGAGTCTTTCAGGATCCGACTGGCTTGGGACAATAGCTGCAGGAG
ATATATCCGGGGAGCTCATTGCTGCTATATTACCAGATATGGCACTGAATCCAATAAATGTCAAGCGACC
TGTAGAGCGAAGATTTCCTATATATAAAGCAGATCTGATACCGTATCAGGACAGTCCTGAAGGTCCAGAC
CATTCTTCTGCTTCATCTGGGGTCCCCAACCCTCCTAAGGCTCGAACTTACACTGAAACTGTCAACCATC
ACTACTTGCTCTTTCAAGACACAGATTTGGGTTCATTCCATGATCTGCTCCGTAGAGAACCAATGCTGCG
CATGCAGGAGGGAGAGGGGCATTCTCAACTCTGCCTGGACAGGCTGCAGCTGGAGGCTATTCATAAGGTA
CGTTTCAGCCCAAACCTGGACTCCTATGGATGGCTGGTATCTGGGGGGCAGTCAGGGCTGGTTCGAATCC
ATTTTGTCCGTGGACTCGCCTCCCCACTGGGCCACCGTATGCAGCTTGAAAGCCGAGCCCACTTCAATGC
TATGTTCCAACCATCCTCCCCCACTAGACGGCCTGGCTTCTCTCCAACCAGCCATCGCCTTCTGCCCACT
CCC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC202276 protein sequence
Red=Cloning site Green=Tags(s)

MDTCGVGYVALGEAGPVGNMTVVDSPGQEVLNQLDVKTSSEMTSAEASVEMSLPTPLPGFEDSPDQRRLP
PEQESLSRLEQPDLSSEMSKVSKPRASKPGRKRGGRTRKGPKRPQQPNPPSAPLVPGLLDQSNPLSTPMP
KKRGRKSKAELLLLKLSKDLDRPESQSPKRPPEDFETPSGERPRRRAAQVALLYLQELAEELSTALPAPV
SCPEGPKVSSPTKPKKIRQPAACPGGEEVDGAPRDEDFFLQVEAEDVEESEGPSESSSEPEPVVPRSTPR
GSTSGKQKPHCRGMAPNGLPDHIMAPVWKCLHLTKDFREQKHSYWEFAEWIPLAWKWHLLSELEAAPYLP
QEEKSPLFSVQREGLPEDGTLYRINRFSSITAHPERWDVSFFTGGPLWALDWCPVPEGAGASQYVALFSS
PDMNETHPLSQLHSGPGLLQLWGLGTLQQESCPGNRAHFVYGIACDNGCIWDLKFCPSGAWELPGTPRKA
PLLPRLGLLALACSDGKVLLFSLPHPEALLAQQPPDAVKPAIYKVQCVATLQVGSMQATDPSECGQCLSL
AWMPTRPHQHLAAGYYNGMVVFWNLPTNSPLQRIRLSDGSLKLYPFQCFLAHDQAVRTLQWCKANSHFLV
SAGSDRKIKFWDLRRPYEPINSIKRFLSTELAWLLPYNGVTVAQDNCYASYGLCGIHYIDAGYLGFKAYF
TAPRKGTVWSLSGSDWLGTIAAGDISGELIAAILPDMALNPINVKRPVERRFPIYKADLIPYQDSPEGPD
HSSASSGVPNPPKARTYTETVNHHYLLFQDTDLGSFHDLLRREPMLRMQEGEGHSQLCLDRLQLEAIHKV
RFSPNLDSYGWLVSGGQSGLVRIHFVRGLASPLGHRMQLESRAHFNAMFQPSSPTRRPGFSPTSHRLLPT
P

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001521
ORF Size 2733 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001521.3
RefSeq Size 3976 bp
RefSeq ORF 2736 bp
Locus ID 2976
UniProt ID Q8WUA4
Cytogenetics 2p23.3
Domains WD40
Protein Families Transcription Factors
MW 100.7 kDa
Gene Summary Required for RNA polymerase III-mediated transcription. Component of TFIIIC that initiates transcription complex assembly on tRNA and is required for transcription of 5S rRNA and other stable nuclear and cytoplasmic RNAs. May play a direct role in stabilizing interactions of TFIIIC2 with TFIIIC1.[UniProtKB/Swiss-Prot Function]

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