GTF3C2 (NM_001521) Human Tagged ORF Clone

CAT#: RC202276

GTF3C2 (Myc-DDK-tagged)-Human general transcription factor IIIC, polypeptide 2, beta 110kDa (GTF3C2), transcript variant 1

Product Images

Circular map for RC202276

• 

  Western blot validation of overexpression lysate (Cat# LY422140) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC221338 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified GTF3C2 protein (Cat# TP302276). The protein was produced from HEK293T cells transfected with GTF3C2 cDNA clone (Cat# RC202276) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GTF3C2
• Synonyms: TFIIIC-BETA; TFIIIC110
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC202276 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATACCTGCGGGGTCGGCTATGTTGCCCTGGGGGAGGCCGGCCCCGTGGGGAACATGACTGTGGTAG
  ACTCTCCTGGACAAGAGGTGCTAAATCAGCTTGATGTCAAGACCTCTTCAGAAATGACCAGTGCAGAGGC
  TTCCGTAGAGATGTCATTACCTACCCCTTTGCCTGGATTTGAGGATTCTCCTGATCAGAGGAGGCTCCCT
  CCAGAGCAGGAAAGCCTCTCCAGACTGGAACAGCCAGATCTTTCTTCAGAGATGTCAAAGGTCTCAAAGC
  CTAGGGCCTCAAAGCCTGGCCGGAAGAGAGGTGGTAGGACACGAAAAGGCCCCAAAAGGCCCCAACAGCC
  TAATCCTCCATCAGCCCCACTGGTTCCTGGTCTCTTAGATCAATCCAACCCTCTGTCCACCCCCATGCCT
  AAGAAACGAGGTCGAAAGTCCAAGGCAGAGCTGCTGCTGCTGAAGTTGTCAAAAGACCTAGATCGGCCAG
  AATCTCAATCTCCAAAGAGGCCCCCTGAGGACTTTGAGACCCCTTCTGGGGAACGACCCCGCCGAAGGGC
  TGCCCAAGTGGCACTTCTGTATCTTCAGGAACTGGCTGAAGAGCTCTCAACAGCCCTGCCTGCCCCTGTG
  TCCTGTCCTGAGGGCCCCAAGGTGAGCAGCCCCACCAAACCGAAGAAGATCCGGCAGCCAGCAGCCTGTC
  CAGGTGGAGAAGAGGTGGATGGTGCTCCACGGGATGAAGACTTTTTTCTCCAGGTTGAGGCTGAAGATGT
  GGAAGAAAGTGAGGGCCCAAGTGAGAGCTCATCTGAACCTGAGCCTGTAGTGCCCCGAAGCACCCCACGA
  GGATCTACTTCAGGGAAACAGAAACCACACTGCCGAGGAATGGCTCCCAATGGCTTACCAGATCATATCA
  TGGCTCCTGTTTGGAAGTGCCTCCATCTCACCAAGGACTTCCGAGAGCAGAAACATTCATACTGGGAGTT
  TGCTGAGTGGATTCCTTTAGCCTGGAAGTGGCACTTGTTATCTGAGCTTGAGGCCGCTCCCTACCTGCCC
  CAGGAGGAGAAGTCTCCATTGTTTTCTGTACAACGTGAAGGGCTACCTGAAGATGGCACCCTCTACCGAA
  TAAACAGATTTAGCTCGATCACAGCACATCCAGAGCGCTGGGATGTGTCCTTCTTCACGGGGGGACCGCT
  CTGGGCTCTGGACTGGTGCCCAGTGCCAGAGGGGGCAGGAGCCTCGCAATATGTGGCTCTTTTCTCCAGC
  CCTGACATGAATGAGACACACCCACTGAGCCAGCTTCATTCGGGTCCTGGGCTGCTCCAGCTCTGGGGCC
  TTGGGACCTTGCAGCAAGAAAGCTGTCCTGGCAACAGGGCCCACTTTGTCTATGGGATTGCTTGTGACAA
  CGGCTGCATCTGGGACCTCAAGTTCTGCCCCAGTGGAGCATGGGAACTTCCAGGCACCCCTCGGAAGGCT
  CCTCTCCTGCCCCGGTTGGGTCTCTTGGCTCTGGCCTGCTCAGACGGGAAAGTACTGCTATTCAGTCTAC
  CCCATCCGGAGGCCCTGCTGGCTCAGCAACCCCCAGATGCAGTGAAGCCTGCCATATATAAGGTACAATG
  TGTGGCAACTCTGCAGGTGGGGTCTATGCAAGCTACAGACCCCTCTGAGTGTGGTCAGTGCCTTAGCCTG
  GCCTGGATGCCTACCAGGCCCCACCAACACCTAGCTGCTGGATATTATAATGGCATGGTGGTTTTCTGGA
  ACCTTCCCACTAACTCACCCCTGCAGCGGATACGGCTCTCTGATGGCTCCTTAAAGCTCTACCCCTTCCA
  GTGTTTCCTAGCCCATGACCAGGCTGTGCGTACCCTTCAATGGTGCAAAGCTAACAGCCATTTCCTTGTC
  TCTGCGGGGAGTGACCGGAAAATCAAATTCTGGGACCTTCGACGTCCTTACGAACCCATAAACTCTATCA
  AGCGCTTCTTGAGTACAGAACTGGCCTGGCTGCTTCCCTACAATGGTGTCACTGTGGCTCAGGACAACTG
  CTATGCCTCTTATGGACTCTGTGGGATTCATTATATTGACGCTGGTTACCTTGGTTTCAAGGCCTACTTC
  ACTGCTCCTCGAAAAGGCACCGTTTGGAGTCTTTCAGGATCCGACTGGCTTGGGACAATAGCTGCAGGAG
  ATATATCCGGGGAGCTCATTGCTGCTATATTACCAGATATGGCACTGAATCCAATAAATGTCAAGCGACC
  TGTAGAGCGAAGATTTCCTATATATAAAGCAGATCTGATACCGTATCAGGACAGTCCTGAAGGTCCAGAC
  CATTCTTCTGCTTCATCTGGGGTCCCCAACCCTCCTAAGGCTCGAACTTACACTGAAACTGTCAACCATC
  ACTACTTGCTCTTTCAAGACACAGATTTGGGTTCATTCCATGATCTGCTCCGTAGAGAACCAATGCTGCG
  CATGCAGGAGGGAGAGGGGCATTCTCAACTCTGCCTGGACAGGCTGCAGCTGGAGGCTATTCATAAGGTA
  CGTTTCAGCCCAAACCTGGACTCCTATGGATGGCTGGTATCTGGGGGGCAGTCAGGGCTGGTTCGAATCC
  ATTTTGTCCGTGGACTCGCCTCCCCACTGGGCCACCGTATGCAGCTTGAAAGCCGAGCCCACTTCAATGC
  TATGTTCCAACCATCCTCCCCCACTAGACGGCCTGGCTTCTCTCCAACCAGCCATCGCCTTCTGCCCACT
  CCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC202276 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MDTCGVGYVALGEAGPVGNMTVVDSPGQEVLNQLDVKTSSEMTSAEASVEMSLPTPLPGFEDSPDQRRLP
  PEQESLSRLEQPDLSSEMSKVSKPRASKPGRKRGGRTRKGPKRPQQPNPPSAPLVPGLLDQSNPLSTPMP
  KKRGRKSKAELLLLKLSKDLDRPESQSPKRPPEDFETPSGERPRRRAAQVALLYLQELAEELSTALPAPV
  SCPEGPKVSSPTKPKKIRQPAACPGGEEVDGAPRDEDFFLQVEAEDVEESEGPSESSSEPEPVVPRSTPR
  GSTSGKQKPHCRGMAPNGLPDHIMAPVWKCLHLTKDFREQKHSYWEFAEWIPLAWKWHLLSELEAAPYLP
  QEEKSPLFSVQREGLPEDGTLYRINRFSSITAHPERWDVSFFTGGPLWALDWCPVPEGAGASQYVALFSS
  PDMNETHPLSQLHSGPGLLQLWGLGTLQQESCPGNRAHFVYGIACDNGCIWDLKFCPSGAWELPGTPRKA
  PLLPRLGLLALACSDGKVLLFSLPHPEALLAQQPPDAVKPAIYKVQCVATLQVGSMQATDPSECGQCLSL
  AWMPTRPHQHLAAGYYNGMVVFWNLPTNSPLQRIRLSDGSLKLYPFQCFLAHDQAVRTLQWCKANSHFLV
  SAGSDRKIKFWDLRRPYEPINSIKRFLSTELAWLLPYNGVTVAQDNCYASYGLCGIHYIDAGYLGFKAYF
  TAPRKGTVWSLSGSDWLGTIAAGDISGELIAAILPDMALNPINVKRPVERRFPIYKADLIPYQDSPEGPD
  HSSASSGVPNPPKARTYTETVNHHYLLFQDTDLGSFHDLLRREPMLRMQEGEGHSQLCLDRLQLEAIHKV
  RFSPNLDSYGWLVSGGQSGLVRIHFVRGLASPLGHRMQLESRAHFNAMFQPSSPTRRPGFSPTSHRLLPT
  P
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001521
• ORF Size: 2733 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001521.3
• RefSeq Size: 3976 bp
• RefSeq ORF: 2736 bp
• Locus ID: 2976
• UniProt ID: Q8WUA4
• Cytogenetics: 2p23.3
• Domains: WD40
• Protein Families: Transcription Factors
• MW: 100.7 kDa
• Gene Summary: Required for RNA polymerase III-mediated transcription. Component of TFIIIC that initiates transcription complex assembly on tRNA and is required for transcription of 5S rRNA and other stable nuclear and cytoplasmic RNAs. May play a direct role in stabilizing interactions of TFIIIC2 with TFIIIC1.[UniProtKB/Swiss-Prot Function]