PPM1D (NM_003620) Human Untagged Clone

CAT#: SC117895

PPM1D (untagged)-Human protein phosphatase, Mg2+/Mn2+ dependent, 1D (PPM1D)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: PPM1D
• Synonyms: IDDGIP; JDVS; PP2C-DELTA; WIP1
• Vector: pCMV6-XL5
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_003620, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCGGGGCTGTACTCGCTGGGAGTGAGCGTCTTCTCCGACCAGGGCGGGAGGAAGTACATGGAGGACG
  TTACTCAAATCGTTGTGGAGCCCGAACCGACGGCTGAAGAAAAGCCCTCGCCGCGGCGGTCGCTGTCTCA
  GCCGTTGCCTCCGCGGCCGTCGCCGGCCGCCCTTCCCGGCGGCGAAGTCTCGGGGAAAGGCCCAGCGGTG
  GCAGCCCGAGAGGCTCGCGACCCTCTCCCGGACGCCGGGGCCTCGCCGGCACCTAGCCGCTGCTGCCGCC
  GCCGTTCCTCCGTGGCCTTTTTCGCCGTGTGCGACGGGCACGGCGGGCGGGAGGCGGCACAGTTTGCCCG
  GGAGCACTTGTGGGGTTTCATCAAGAAGCAGAAGGGTTTCACCTCGTCCGAGCCGGCTAAGGTTTGCGCT
  GCCATCCGCAAAGGCTTTCTCGCTTGTCACCTTGCCATGTGGAAGAAACTGGCGGAATGGCCAAAGACTA
  TGACGGGTCTTCCTAGCACATCAGGGACAACTGCCAGTGTGGTCATCATTCGGGGCATGAAGATGTATGT
  AGCTCACGTAGGTGACTCAGGGGTGGTTCTTGGAATTCAGGATGACCCGAAGGATGACTTTGTCAGAGCT
  GTGGAGGTGACACAGGACCATAAGCCAGAACTTCCCAAGGAAAGAGAACGAATCGAAGGACTTGGTGGGA
  GTGTAATGAACAAGTCTGGGGTGAATCGTGTAGTTTGGAAACGACCTCGACTCACTCACAATGGACCTGT
  TAGAAGGAGCACAGTTATTGACCAGATTCCTTTTCTGGCAGTAGCAAGAGCACTTGGTGATTTGTGGAGC
  TATGATTTCTTCAGTGGTGAATTTGTGGTGTCACCTGAACCAGACACAAGTGTCCACACTCTTGACCCTC
  AGAAGCACAAGTATATTATATTGGGGAGTGATGGACTTTGGAATATGATTCCACCACAAGATGCCATCTC
  AATGTGCCAGGACCAAGAGGAGAAAAAATACCTGATGGGTGAGCATGGACAATCTTGTGCCAAAATGCTT
  GTGAATCGAGCATTGGGCCGCTGGAGGCAGCGTATGCTCCGAGCAGATAACACTAGTGCCATAGTAATCT
  GCATCTCTCCAGAAGTGGACAATCAGGGAAACTTTACCAATGAAGATGAGTTATACCTGAACCTGACTGA
  CAGCCCTTCCTATAATAGTCAAGAAACCTGTGTGATGACTCCTTCCCCATGTTCTACACCACCAGTCAAG
  TCACTGGAGGAGGATCCATGGCCAAGGGTGAATTCTAAGGACCATATACCTGCCCTGGTTCGTAGCAATG
  CCTTCTCAGAGAATTTTTTAGAGGTTTCAGCTGAGATAGCTCGAGAGAATGTCCAAGGTGTAGTCATACC
  CTCAAAAGATCCAGAACCACTTGAAGAAAATTGCGCTAAAGCCCTGACTTTAAGGATACATGATTCTTTG
  AATAATAGCCTTCCAATTGGCCTTGTGCCTACTAATTCAACAAACACTGTCATGGACCAAAAAAATTTGA
  AGATGTCAACTCCTGGCCAAATGAAAGCCCAAGAAATTGAAAGAACCCCTCCAACAAACTTTAAAAGGAC
  ATTAGAAGAGTCCAATTCTGGCCCCCTGATGAAGAAGCATAGACGAAATGGCTTAAGTCGAAGTAGTGGT
  GCTCAGCCTGCAAGTCTCCCCACAACCTCACAGCGAAAGAACTCTGTTAAACTCACCATGCGACGCAGAC
  TTAGGGGCCAGAAGAAAATTGGAAATCCTTTACTTCATCAACACAGGAAAACTGTTTGTGTTTGCTGA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_003620 unedited
  GTAATACGACTCACTATAGGGCGGCCGCGAATTCGGCACGAGGCCTTCTCCGGGTCCGCC
  CCCTCCCCCTTCTCGGCGTCGTCGAAGATAAACAATAGTTGGCCGGCGAGCGCCTAGTGT
  GTCTCCCGCCGCCGGATTCGGCGGGCTGCGTGGGACCGGCGGGATCCCGGCCAGCCGGCC
  ATGGCGGGGCTGTACTCGCTGGGAGTGAGCGTCTTCTCCGACCAGGGCGGGAGGAAGTAC
  ATGGAGGACGTTACTCAAATCGTTGTGGAGCCCGAACCGACGGCTGAAGAAAAGCCCTCG
  CCGCGGCGGTCGCTGTCTCAGCCGTTGCCTCCGCGGCCGTCGCCGGCCGCCCTTCCCGGC
  GGCGAAGTCTCGGGGAAAGGCCCAGCGGTGGCAGCCCGAGAGGCTCGCGACCCTCTCCCG
  GACGCCGGGGCCTCGCCGGCACCTAGCCGCTGCTGCCGCCGCCGTTCCTCCGTGGCCTTT
  TTCGCCGTGTGCGACGGGCACGGCGGGCGGGAGGCGGCACAGTTTGCCCGGGAGCACTTG
  TGGGGTTTCATCAAGAAGCAGAAGGGTTTCACCTCGTCCGAGCCGGCTAAGGTTTGCGCT
  GCCATCCGCAAAGGCTTTCTCGCTTGTCACCTTGCCATGTGGAAGAAACTGGCGGAATGG
  CCAAAGACTATGACGGGTCTTCCTAGCACATCAGGGACAACTGCCAGTGTGGTCATCATT
  CGGNGCATGAAGATGTATGTAGCTCACGTAGGTGACTCAGGGNGTGGCTTCTTGGNATTC
  AGGATGACCCGAAGGATGACTNTGTCAGAGCTGTGGGAGTGACACAGGACCCATAGCCAG
  AACTTCCCAGGNNAAGAGACGAATCGATGACTTGGTGGGAGTGTAAGAACAGTCCTGGGT
  GAATCGCGGTATTTGGGAACCGACTCGACTACTCACATGGACCTGNTAGAAGAGCCAGTT
  ATGACAAC
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_003620 unedited
  ACCGCGGCCGCAATTTAGAGTCGAGCCCTTTTCTTTTTTTTTTTCTTTTTCTTTTTTTTT
  TTTTTTTTTTTTTTTCTTTTTGGGGGACCGGTCACTGGTTTATTAAAAGAACCTTTTCCC
  CTATAATCAATGAAAACTGTTTGATCTAAAAAAATTTAAAAAATGGCGGGAATTAACCGC
  CCCCTTTGTTTTTGAAATAACAAGGACAATTTCCCCTTTTTTAACCCAACCCGAAAAGGG
  GGTAATTCCTTTAAAACCTAAATATGCCATTGGTCTGGGATGAAAAAAAAGGGAATTGCC
  CTTAAACTGTTTTAAGGCAACACCCTTCCTTTTATGCCGAAAACTTAATAAGCCCTTTCG
  CAGACCCAGGCTTTAAAAAAACGCCCCTGGTGGAAAACACACCGGCTAGCCCCTTAATTG
  CGCCAACACCAACTGGTCTCGGCGTTCATCCTTCTATGAAACATTTACCTCCTAATTCCG
  CCATTCCGAAATCAAAACTTTTGTTTAACCGACCCCTTGGCTTCTTCAAAACATTAAGCG
  ACACCCCCGCCGCCCTGAAAATACACCACTTTTCCCCCCCGCCTAATAATTTACCTTACA
  AAGTCGTACCGCTTCCAACACAACGCTCCATACAAATTCTCTACCTACCCCTCTCACACG
  TCTACCCCGTTTTTCAAAACCCAACAAAATCTTTGGATTTCTCCCAACCCCCATCCCTTT
  TGTACCCCCTCCTCGTATTACACACCTTTAACTCCGGTCCTTCCACACTGAATTTCGCGC
  CTTCCCACTCCCCCATTTCTCGCGTTCTTTCCTTCACCCTTTAACCCCTTTCCCTTTACC
  CCCCCCCGCCCGATAAACCGCCCATCCAAAAGAAACATGTTCTCCTTTATCCCCCCCTCA
  AAAAGGTACACTGCCCCACTTAAG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_003620
• Insert Size: 2840 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003620.2, NP_003611.1
• RefSeq Size: 3163 bp
• RefSeq ORF: 1818 bp
• Locus ID: 8493
• UniProt ID: O15297
• Cytogenetics: 17q23.2
• Domains: PP2C
• Protein Families: Druggable Genome, Phosphatase
• Protein Pathways: p53 signaling pathway
• Gene Summary: The protein encoded by this gene is a member of the PP2C family of Ser/Thr protein phosphatases. PP2C family members are known to be negative regulators of cell stress response pathways. The expression of this gene is induced in a p53-dependent manner in response to various environmental stresses. While being induced by tumor suppressor protein TP53/p53, this phosphatase negatively regulates the activity of p38 MAP kinase, MAPK/p38, through which it reduces the phosphorylation of p53, and in turn suppresses p53-mediated transcription and apoptosis. This phosphatase thus mediates a feedback regulation of p38-p53 signaling that contributes to growth inhibition and the suppression of stress induced apoptosis. This gene is located in a chromosomal region known to be amplified in breast cancer. The amplification of this gene has been detected in both breast cancer cell line and primary breast tumors, which suggests a role of this gene in cancer development. [provided by RefSeq, Jul 2008]