LCMT2 (NM_014793) Human Untagged Clone

CAT#: SC114825

LCMT2 (untagged)-Human leucine carboxyl methyltransferase 2 (LCMT2)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: LCMT2
• Synonyms: PPM2; TYW4
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_014793, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGGCCCCCGGAGCCGTGAGCGTCGGGCAGGCGCGGTACAGAACACCAACGACAGCAGCGCCCTCAGCA
  AGCGTTCCCTGGCCGCGCGCGGGTACGTGCAGGACCCCTTTGCCGCGTTGCTGGTTCCGGGCGCGGCGCG
  CCGCGCACCGCTCATTCACCGAGGCTACTACGTCCGCGCACGCGCCGTGAGGCACTGCGTGCGCGCTTTT
  TTGGAGCAGATTGGCGCGCCCCAGGCCGCGCTTCGCGCGCAGATCTTGTCTCTCGGCGCTGGCTTCGACT
  CGCTCTATTTTCGCTTAAAAACCGCGGGCCGCCTGGCCCGGGCTGCAGTCTGGGAGGTGGATTTTCCGGA
  CGTGGCGCGGCGCAAAGCAGAAAGGATTGGAGAGACGCCAGAGCTGTGCGCGTTAACCGGGCCTTTCGAG
  AGGGGGGAGCCCGCGTCCGCGCTGTGCTTTGAGAGCGCAGACTACTGCATCCTGGGTCTGGACTTGCGGC
  AGCTCCAGCGAGTGGAGGAGGCCCTGGGCGCCGCGGGGCTCGACGCAGCCTCACCCACTCTGCTCCTGGC
  CGAGGCGGTGCTGACCTACCTCGAGCCGGAGAGTGCCGCGGCCCTCATCGCCTGGGCAGCCCAGCGTTTT
  CCTAATGCCCTTTTCGTGGTCTATGAGCAGATGAGGCCTCAAGACGCCTTTGGCCAGTTCATGCTGCAAC
  ATTTTCGGCAGCTAAACTCCCCCCTGCATGGCCTGGAGCGTTTTCCTGACGTGGAGGCGCAGCGGCGCCG
  CTTCCTTCAAGCTGGCTGGACCGCCTGCGGTGCCGTGGACATGAATGAATTCTATCACTGCTTTCTTCCC
  GCAGAAGAACGCCGGCGGGTGGAAAATATTGAACCCTTTGACGAATTTGAGGAGTGGCATCTGAAGTGCG
  CCCATTATTTCATTCTGGCAGCTTCTAGGGGAGACACCCTCTCCCACACCCTAGTGTTTCCATCCTCAGA
  GGCATTTCCTCGCGTAAATCCTGCTTCGCCTTCAGGGGTATTCCCTGCCAGCGTAGTCAGTAGCGAGGGC
  CAGGTCCCAAACCTGAAGAGATATGGCCACGCCTCTGTCTTCTTGAGCCCAGACGTTATTCTCAGTGCAG
  GAGGATTTGGAGAGCAGGAGGGGCGGCACTGCCGAGTGAGCCAGTTTCACTTGCTCTCAAGAGATTGTGA
  CTCTGAATGGAAAGGCAGCCAAATAGGCAGTTGTGGGACTGGAGTTCAGTGGGATGGACGCCTTTATCAC
  ACCATGACAAGACTCTCAGAGAGTCGGGTTCTGGTTCTGGGAGGGAGACTGTCCCCAGTAAGTCCAGCCT
  TGGGGGTTCTCCAGCTTCATTTTTTTAAGAGTGAGGATAATAACACTGAGGACCTGAAAGTGACAATAAC
  AAAGGCTGGCCGAAAGGATGATTCCACTTTGTGTTGTTGGCGGCATTCAACAACAGAAGTGTCCTGTCAG
  AATCAGGAATATTTGTTTGTGTATGGGGGTCGAAGCGTGGTGGAACCTGTACTAAGTGACTGGCATTTCC
  TCCATGTAGGGACAATGGCTTGGGTCAGGATCCCAGTGGAGGGAGAAGTACCTGAAGCCCGGCATTCTCA
  CAGTGCCTGCACTTGGCAAGGGGGAGCCCTTATTGCTGGAGGTCTCGGGGCTTCTGAGGAGCCATTGAAC
  TCTGTGCTCTTTCTGAGACCAATCTCTTGTGGATTCCTCTGGGAGTCAGTAGACATCCAGCCTCCCATTA
  CCCCAAGGTACTCCCACACAGCTCATGTGCTCAATGGAAAGCTGTTACTGGTTGGAGGGATCTGGATTCA
  TTCCTCCTCATTTCCTGGAGTGACTGTGATCAATTTGACTACAGGATTGAGCTCTGAGTATCAGATTGAC
  ACAACATATGTGCCATGGCCATTAATGTTACACAACCATACTAGTATCCTTCTTCCTGAAGAGCAACAGC
  TCCTGCTCCTTGGAGGTGGTGGGAACTGCTTTTCCTTTGGTACCTACTTCAACCCCCATACAGTCACATT
  AGACCTTTCTTCCTTAAGTGCTGGGCAGTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_014793 unedited
  TTTGTAATACGAACTCACTATAGGGCGGCCGCGATTCGGCACGAGGGANAGAGAGAGAGA
  GAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGAGA
  GAGCCAAGGGGCGGCTCACAACCGTGACAGACTGCCATTCCTGAGTCTCTTCTGGCCATG
  GGCCCCCGGAGCCGTGAGCGTCGGGCAGGCGCGGTACAGAACACCAACGACAGCAGCGCC
  CTCAGCAAGCGTTCCCTGGCCGCGCGCGGGTACGTGCAGGACCCCTTTGCCGCGTTGCTG
  GTTCCGGGCGCGGCGCGCCGCGCACCGCTCATTCACCGAGGCTACTACGTCCGCGCACGC
  GCCGTGAGGCACTGCGTGCGCGCTTTTTTGGAGCAGATTGGCGCGCCCCAGGCCGCGCTT
  CGCGCGCAGATCTTGTCTCTCGGCGCTGGCTTCGACTCGCTCTATTTTCGCTTAAAAACC
  GCGGGCCGCCTGGCCCGGGCTGCAGTCTGGGAGGTGGATTTTCCGGACGTGGCGCGGCGC
  AAAGCAGAAAGGATTGGAGAGACGCCAGAGCTGTGCGCGTTAACCGGGCCTTTCGAGAGG
  GGGGAGCCCGCGTCCGCGCTGTGCTTTGAGAGCGCAGAACTACTGCATCCTGGGTCTGGA
  CTTGCGGCAGCTCCAGCGAGTGGGAGGAAGCCCTGGCGCCCCNNNGGGGCTCGACGCAGC
  CTCACCCACTCTGCTCCTGGCGCGAAGCGGGTGCTGACCTACCTCGAGCCCGGGAGAGTG
  GCCGGCGGCCCTCATCGCCTGGGCAGNCCCAGCGNTNTCCTATGCCCTTTTNNCGGGGTC
  TATGAAGCAAATGAGGCCTCAAGAACGCTTTGGCCCAGNTCATGCTGCANCATTNTTCGC
  AGCTAAACTCCCCTGCATGGCCTGGAGCCTTCCG
  
• Restriction Sites: NotI-NotI
• ACCN: NM_014793
• Insert Size: 3340 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014793.1, NP_055608.1
• RefSeq Size: 2860 bp
• RefSeq ORF: 2061 bp
• Locus ID: 9836
• UniProt ID: O60294
• Cytogenetics: 15q15.3
• Domains: LCM
• Protein Families: Druggable Genome
• Protein Pathways: Alzheimer's disease, Androgen and estrogen metabolism, Cardiac muscle contraction, Histidine metabolism, Huntington's disease, Metabolic pathways, Oxidative phosphorylation, Parkinson's disease, Selenoamino acid metabolism, Tyrosine metabolism
• Gene Summary: The protein encoded by this intronless gene belongs to the highly variable methyltransferase superfamily. This gene is the inferred homolog of the Saccharomyces cerevisiae carboxymethyltransferase gene PPM2 that is essential for the synthesis of the hypermodified guanosine Wybutosine (yW). [provided by RefSeq, Jul 2008]