LCMT2 (NM_014793) Human Tagged ORF Clone

CAT#: RC202227

LCMT2 (Myc-DDK-tagged)-Human leucine carboxyl methyltransferase 2 (LCMT2)

Product Images

Circular map for RC202227

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  Western blot validation of overexpression lysate (Cat# LY415004) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC202227 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified LCMT2 protein (Cat# TP302227). The protein was produced from HEK293T cells transfected with LCMT2 cDNA clone (Cat# RC202227) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: LCMT2
• Synonyms: PPM2; TYW4
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC202227 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCCCCCGGAGCCGTGAGCGTCGGGCAGGCGCGGTACAGAACACCAACGACAGCAGCGCCCTCAGCA
  AGCGTTCCCTGGCCGCGCGCGGGTACGTGCAGGACCCCTTTGCCGCGTTGCTGGTTCCGGGCGCGGCGCG
  CCGCGCACCGCTCATTCACCGAGGCTACTACGTCCGCGCACGCGCCGTGAGGCACTGCGTGCGCGCTTTT
  TTGGAGCAGATTGGCGCGCCCCAGGCCGCGCTTCGCGCGCAGATCTTGTCTCTCGGCGCTGGCTTCGACT
  CGCTCTATTTTCGCTTAAAAACCGCGGGCCGCCTGGCCCGGGCTGCAGTCTGGGAGGTGGATTTTCCGGA
  CGTGGCGCGGCGCAAAGCAGAAAGGATTGGAGAGACGCCAGAGCTGTGCGCGTTAACCGGGCCTTTCGAG
  AGGGGGGAGCCCGCGTCCGCGCTGTGCTTTGAGAGCGCAGACTACTGCATCCTGGGTCTGGACTTGCGGC
  AGCTCCAGCGAGTGGAGGAGGCCCTGGGCGCCGCGGGGCTCGACGCAGCCTCACCCACTCTGCTCCTGGC
  CGAGGCGGTGCTGACCTACCTCGAGCCGGAGAGTGCCGCGGCCCTCATCGCCTGGGCAGCCCAGCGTTTT
  CCTAATGCCCTTTTCGTGGTCTATGAGCAGATGAGGCCTCAAGACGCCTTTGGCCAGTTCATGCTGCAAC
  ATTTTCGGCAGCTAAACTCCCCCCTGCATGGCCTGGAGCGTTTTCCTGACGTGGAGGCGCAGCGGCGCCG
  CTTCCTTCAAGCTGGCTGGACCGCCTGCGGTGCCGTGGACATGAATGAATTCTATCACTGCTTTCTTCCC
  GCAGAAGAACGCCGGCGGGTGGAAAATATTGAACCCTTTGACGAATTTGAGGAGTGGCATCTGAAGTGCG
  CCCATTATTTCATTCTGGCAGCTTCTAGGGGAGACACCCTCTCCCACACCCTAGTGTTTCCATCCTCAGA
  GGCATTTCCTCGCGTAAATCCTGCTTCGCCTTCAGGGGTATTCCCTGCCAGCGTAGTCAGTAGCGAGGGC
  CAGGTCCCAAACCTGAAGAGATATGGCCACGCCTCTGTCTTCTTGAGCCCAGACGTTATTCTCAGTGCAG
  GAGGATTTGGAGAGCAGGAGGGGCGGCACTGCCGAGTGAGCCAGTTTCACTTGCTCTCAAGAGATTGTGA
  CTCTGAATGGAAAGGCAGCCAAATAGGCAGTTGTGGGACTGGAGTTCAGTGGGATGGACGCCTTTATCAC
  ACCATGACAAGACTCTCAGAGAGTCGGGTTCTGGTTCTGGGAGGGAGACTGTCCCCAGTAAGTCCAGCCT
  TGGGGGTTCTCCAGCTTCATTTTTTTAAGAGTGAGGATAATAACACTGAGGACCTGAAAGTGACAATAAC
  AAAGGCTGGCCGAAAGGATGATTCCACTTTGTGTTGTTGGCGGCATTCAACAACAGAAGTGTCCTGTCAG
  AATCAGGAATATTTGTTTGTGTATGGGGGTCGAAGCGTGGTGGAACCTGTACTAAGTGACTGGCATTTCC
  TCCATGTAGGGACAATGGCTTGGGTCAGGATCCCAGTGGAGGGAGAAGTACCTGAAGCCCGGCATTCTCA
  CAGTGCCTGCACTTGGCAAGGGGGAGCCCTTATTGCTGGAGGTCTCGGGGCTTCTGAGGAGCCATTGAAC
  TCTGTGCTCTTTCTGAGACCAATCTCTTGTGGATTCCTCTGGGAGTCAGTAGACATCCAGCCTCCCATTA
  CCCCAAGGTACTCCCACACAGCTCATGTGCTCAATGGAAAGCTGTTACTGGTTGGAGGGATCTGGATTCA
  TTCCTCCTCATTTCCTGGAGTGACTGTGATCAATTTGACTACAGGATTGAGCTCTGAGTATCAGATTGAC
  ACAACATATGTGCCATGGCCATTAATGTTACACAACCATACTAGTATCCTTCTTCCTGAAGAGCAACAGC
  TCCTGCTCCTTGGAGGTGGTGGGAACTGCTTTTCCTTTGGTACCTACTTCAACCCCCATACAGTCACATT
  AGACCTTTCTTCCTTAAGTGCTGGGCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC202227 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGPRSRERRAGAVQNTNDSSALSKRSLAARGYVQDPFAALLVPGAARRAPLIHRGYYVRARAVRHCVRAF
  LEQIGAPQAALRAQILSLGAGFDSLYFRLKTAGRLARAAVWEVDFPDVARRKAERIGETPELCALTGPFE
  RGEPASALCFESADYCILGLDLRQLQRVEEALGAAGLDAASPTLLLAEAVLTYLEPESAAALIAWAAQRF
  PNALFVVYEQMRPQDAFGQFMLQHFRQLNSPLHGLERFPDVEAQRRRFLQAGWTACGAVDMNEFYHCFLP
  AEERRRVENIEPFDEFEEWHLKCAHYFILAASRGDTLSHTLVFPSSEAFPRVNPASPSGVFPASVVSSEG
  QVPNLKRYGHASVFLSPDVILSAGGFGEQEGRHCRVSQFHLLSRDCDSEWKGSQIGSCGTGVQWDGRLYH
  TMTRLSESRVLVLGGRLSPVSPALGVLQLHFFKSEDNNTEDLKVTITKAGRKDDSTLCCWRHSTTEVSCQ
  NQEYLFVYGGRSVVEPVLSDWHFLHVGTMAWVRIPVEGEVPEARHSHSACTWQGGALIAGGLGASEEPLN
  SVLFLRPISCGFLWESVDIQPPITPRYSHTAHVLNGKLLLVGGIWIHSSSFPGVTVINLTTGLSSEYQID
  TTYVPWPLMLHNHTSILLPEEQQLLLLGGGGNCFSFGTYFNPHTVTLDLSSLSAGQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_014793
• ORF Size: 2058 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_014793.5
• RefSeq Size: 2860 bp
• RefSeq ORF: 2061 bp
• Locus ID: 9836
• UniProt ID: O60294
• Cytogenetics: 15q15.3
• Domains: LCM
• Protein Families: Druggable Genome
• Protein Pathways: Alzheimer's disease, Androgen and estrogen metabolism, Cardiac muscle contraction, Histidine metabolism, Huntington's disease, Metabolic pathways, Oxidative phosphorylation, Parkinson's disease, Selenoamino acid metabolism, Tyrosine metabolism
• MW: 75.6 kDa
• Gene Summary: The protein encoded by this intronless gene belongs to the highly variable methyltransferase superfamily. This gene is the inferred homolog of the Saccharomyces cerevisiae carboxymethyltransferase gene PPM2 that is essential for the synthesis of the hypermodified guanosine Wybutosine (yW). [provided by RefSeq, Jul 2008]