EXOSC1 (NM_016046) Human Untagged Clone

CAT#: SC114517

EXOSC1 (untagged)-Human exosome component 1 (EXOSC1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: EXOSC1
• Synonyms: CGI-108; CSL4; Csl4p; p13; PCH1F; SKI4; Ski4p
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_016046, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCGCCACCTGTGAGATACTGCATCCCCGGCGAACGTCTGTGTAACTTGGAGGAGGGCAGCCCGGGCA
  GCGGCACCTACACCCGCCACGGCTACATCTTTTCGTCGCTTGCCGGCTGTCTGATGAAGAGCAGCGAGAA
  TGGCGCGCTTCCAGTGGTGTCTGTAGTGAGAGAAACAGAGTCCCAGTTACTGCCAGATGTGGGAGCTATT
  GTAACCTGTAAGGTCTCTAGCATCAATTCACGCTTTGCCAAAGTACACATCCTGTATGTGGGGTCCATGC
  CTCTTAAGAACTCTTTTCGAGGAACTATCCGCAAGGAAGATGTCCGAGCAACTGAAAAAGACAAGGTTGA
  AATTTATAAGAGTTTCCGCCCAGGTGACATTGTCTTGGCCAAAGTGATCTCCTTAGGTGATGCACAGTCC
  AACTACCTGCTAACCACCGCCGAGAACGAGCTGGGAGTGGTGGTAGCCCACAGTGAGTCAGGTATCCAGA
  TGGTTCCCATCAGCTGGTGTGAGATGCAGTGCCCTAAGACCCACACTAAAGAATTCCGGAAAGTAGCCCG
  AGTACAACCCGAATTCTTGCAGACCTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_016046 unedited
  AAAANNNGGGGGGNNNNNNNNNTNNNNNNGGNCCGGTTCAAAATTGTATACGACTCATAT
  AGGCGGCCGCGAAATTCGCACCAGGGCGCCACCTGTNGACAACTGCATCCCCGGCGAACG
  TCTGTGTAACTTGGAGGAGGGCAGCCCGGGCAGCGGCACCTACACCCGCCACGGCTACAT
  CTTTTCGTCGCTTGCCGGCTGTCTGATGAAGAGCAGCGAGAATGGCGCGCTTCCAGTGGT
  GTCTGTAGTGAGAGAAACAGAGTCCCAGTTACTGCCAGATGTGGGAGCTATTGTAACCTG
  TAAGGTCTCTAGCATCAATTCACGCTTTGCCAAAGTACACATCCTGTATGTGGGGTCCAT
  GCCTCTTAAGAACTCTTTTCGAGGAACTATCCGCAAGGAAGATGTCCGAGCAACTGAAAA
  AGACAAGGTTGAAATTTATAAGAGTTTCCGCCCAGGTGACATTGTCTTGGCCAAAGTGAT
  CTCCTTAGGTGATGCACAGTCCAACTACCTGCTAACCACCGCCGAGAACGAGCTGGGAGT
  GGTGGTAACCCACAGTGAGTCAGGTATCCAGATGGTTCCCATCAGCTGGTGTGAGATGCA
  GTGCCCTAAGACCCACACTAAAGAATTCCGGACAGTAGCCCGAGTACAACCCGAATTCTT
  GCAGACCTAAGAAGCCACTTTTTACTCTATGCAAGTGGTTAAGCTGTTCCTGAGTATAAC
  ACCAAGATGCTGCTGTCTTTATTCAAACACCTGNCGTCGGCCAACAGCCACTTCCAGNCA
  GNTCTTCCAGTTTACGCTGTACATGGAACATGTCTCTGTGCACCTATCAGTGGATTTCAT
  TCTCTTGAGTAATAATCCTATCGTTTNCAGGCATAAAAGGGGGAACTGAACTTACAAAAA
  AACTCGACTTTAGATTGCGGCCGCGATCATAGCTGNTCCTGAAACGAACCGGNGTGGCAT
  CT
  
• Restriction Sites: NotI-NotI
• ACCN: NM_016046
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016046.2, NP_057130.1
• RefSeq Size: 1150 bp
• RefSeq ORF: 588 bp
• Locus ID: 51013
• UniProt ID: Q9Y3B2
• Cytogenetics: 10q24.1
• Protein Pathways: RNA degradation
• Gene Summary: This gene encodes a core component of the exosome. The mammalian exosome is required for rapid degradation of AU rich element-containing RNAs but not for poly(A) shortening. The association of this protein with the exosome is mediated by protein-protein interactions with ribosomal RNA-processing protein 42 and ribosomal RNA-processing protein 46. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jan 2016]
  Transcript Variant: This variant (1) encodes the longest isoform (a).