EXOSC1 (NM_016046) Human Tagged ORF Clone

CAT#: RG206007

EXOSC1 (tGFP-tagged) - Human exosome component 1 (EXOSC1)

Product Images

Circular map for RG206007

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: EXOSC1
• Synonyms: CGI-108; CSL4; Csl4p; p13; PCH1F; SKI4; Ski4p
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG206007 representing NM_016046
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCCACCTGTGAGATACTGCATCCCCGGCGAACGTCTGTGTAACTTGGAGGAGGGCAGCCCGGGCA
  GCGGCACCTACACCCGCCACGGCTACATCTTTTCGTCGCTTGCCGGCTGTCTGATGAAGAGCAGCGAGAA
  TGGCGCGCTTCCAGTGGTGTCTGTAGTGAGAGAAACAGAGTCCCAGTTACTGCCAGATGTGGGAGCTATT
  GTAACCTGTAAGGTCTCTAGCATCAATTCACGCTTTGCCAAAGTACACATCCTGTATGTGGGGTCCATGC
  CTCTTAAGAACTCTTTTCGAGGAACTATCCGCAAGGAAGATGTCCGAGCAACTGAAAAAGACAAGGTTGA
  AATTTATAAGAGTTTCCGCCCAGGTGACATTGTCTTGGCCAAAGTGATCTCCTTAGGTGATGCACAGTCC
  AACTACCTGCTAACCACCGCCGAGAACGAGCTGGGAGTGGTGGTAGCCCACAGTGAGTCAGGTATCCAGA
  TGGTTCCCATCAGCTGGTGTGAGATGCAGTGCCCTAAGACCCACACTAAAGAATTCCGGAAAGTAGCCCG
  AGTACAACCCGAATTCTTGCAGACC
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG206007 representing NM_016046
  Red=Cloning site Green=Tags(s)
  
  MAPPVRYCIPGERLCNLEEGSPGSGTYTRHGYIFSSLAGCLMKSSENGALPVVSVVRETESQLLPDVGAI
  VTCKVSSINSRFAKVHILYVGSMPLKNSFRGTIRKEDVRATEKDKVEIYKSFRPGDIVLAKVISLGDAQS
  NYLLTTAENELGVVVAHSESGIQMVPISWCEMQCPKTHTKEFRKVARVQPEFLQT
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016046
• ORF Size: 585 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_016046.5
• RefSeq Size: 1150 bp
• RefSeq ORF: 588 bp
• Locus ID: 51013
• UniProt ID: Q9Y3B2
• Cytogenetics: 10q24.1
• Protein Pathways: RNA degradation
• Gene Summary: This gene encodes a core component of the exosome. The mammalian exosome is required for rapid degradation of AU rich element-containing RNAs but not for poly(A) shortening. The association of this protein with the exosome is mediated by protein-protein interactions with ribosomal RNA-processing protein 42 and ribosomal RNA-processing protein 46. Alternative splicing of this gene results in multiple transcript variants. [provided by RefSeq, Jan 2016]