CLCN3 (NM_001829) Human Untagged Clone

CAT#: SC110856

CLCN3 (untagged)-Human chloride channel 3 (CLCN3), transcript variant b

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: CLCN3
• Synonyms: ClC-3; CLC3
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF sequence for NM_001829 edited
  GAGGCTTTCCCAGTGTTCTAGTTCGCCCGTGACCCGGAATAATGAGCAAGGAGGGTGTGG
  TGGGTTGAAAGCCATCCTACTTTACTCCCGAGTTAGAGCATGGATTCAGTTTTAGTCTTA
  AGGGGGAAGTGAGATTGGAGATTTTTATTTTTAATTTTGGGCAGAAGCAGGTTGACTCTA
  GGGATCTCCAGAGCGAGAGGATTTAACTTCATGTTGCTCCCGTGTTTGAAGGAGGACAAT
  AAAAGTCCCACCGGGCAAAATTTTCGTAACCTCTGCGGTAGAAAACGTCAGGTATCTTTT
  AAATCGCGATAGTTTTCGCTGTGTCAGGCTTTCTTCGGTGGAGCTCCGAGGGTAGCTAGG
  TTCTAGGTTTGAAACAGATGCAGAATCCAAAGGCAGCGCAAAAAACAGCCACCGATTTTG
  CTATGTCTCTGAGCTGCGAGATAATCAGACAGCTAAATGGAGTCTGAGCAGCTGTTCCAT
  AGAGGCTACTATAGAAACAGCTACAACAGTATAACAAGTGCAAGTAGTGATGAGGAACTT
  TTAGATGGAGCAGGTGTTATTATGGACTTTCAAACATCTGAAGATGACAATTTATTAGAT
  GGTGACACTGCAGTTGGAACTCATTATACAATGACAAATGGAGGCAGCATTAACAGTTCT
  ACACATTTACTGGATCTTTTGGATGAACCAATTCCAGGTGTTGGTACATATGATGATTTC
  CATACTATTGATTGGGTGCGAGAAAAATGTAAAGACAGAGAAAGGCATAGACGGATCAAC
  AGCAAAAAGAAAGAATCAGCATGGGAAATGACAAAAAGTTTGTATGATGCGTGGTCAGGA
  TGGCTAGTAGTAACACTAACAGGATTGGCATCAGGGGCACTGGCCGGATTAATAGACATT
  GCTGCCGATTGGATGACTGACCTAAAGGAGGGCATTTGCCTTAGTGCGTTGTGGTACAAC
  CACGAACAGTGCTGTTGGGGATCTAATGAAACAACATTTGAAGAGAGGGATAAATGTCCA
  CAGTGGAAAACATGGGCAGAATTAATCATAGGTCAAGCAGAGGGTCCTGGTTCTTATATC
  ATGAACTACATAATGTACATCTTCTGGGCCTTGAGTTTTGCCTTTCTTGCAGTTTCCCTG
  GTAAAGGTATTTGCTCCATATGCCTGTGGCTCTGGAATTCCAGAGATTAAAACTATTTTA
  AGTGGATTCATCATCAGAGGTTACTTGGGAAAATGGACTTTAATGATTAAAACCATCACA
  TTAGTCCTGGCTGTGGCATCAGGTTTGAGTTTAGGAAAAGAAGGTCCCCTGGTACATGTT
  GCCTGTTGCTGCGGAAATATCTTTTCCTACCTCTTTCCAAAGTATAGCACAAACGAAGCT
  AAAAAAAGGGAGGTGCTATCAGCTGCCTCAGCTGCAGGGGTTTCTGTAGCTTTTGGTGCA
  CCAATTGGAGGAGTTCTTTTTAGCCTGGAAGAGGTTAGCTATTATTTTCCTCTCAAAACT
  TTATGGAGATCATTTTTTGCTGCTTTAGTGGCTGCATTTGTTTTGAGGTCCATCAATCCA
  TTTGGTAACAGCCGTCTGGTCCTTTTTTATGTGGAGTATCATACACCATGGTACCTTTTT
  GAACTGTTTCCTTTTATTCTTCTAGGGGTATTTGGAGGGCTTTGGGGAGCCTTTTTCATT
  AGGGCAAATATTGCCTGGTGTCGTCGACGCAAGTCCACGAAATTTGGAAAGTATCCCGTT
  CTGGAAGTCATTATTGTTGCAGCCATTACTGCTGTGATAGCCTTCCCTAATCCATACACT
  AGGCTAAACACCAGTGAACTGATCAAAGAGCTTTTTACAGACTGTGGTCCCCTGGAATCC
  TCTTCTCTTTGTGACTACAGAAATGACATGAATGCCAGTAAAATTGTCGATGACATTCCT
  GATCGTCCAGCAGGCATTGGAGTATATTCAGCTATATGGCAGTTATGCCTGGCACTCATA
  TTTAAAATCATAATGACAGTATTCACTTTTGGCATCAAGGTTCCATCAGGCTTGTTCATC
  CCCAGCATGGCCATTGGAGCGATCGCAGGAAGGATTGTGGGGATTGCGGTGGAGCAGCTT
  GCCTACTATCACCACGACTGGTTTATCTTTAAGGAGTGGTGTGAGGTCGGGGCTGATTGC
  ATTACACCTGGCCTTTATGCCATGGTTGGTGCTGCTGCATGCTTAGGTGGTGTGACAAGA
  ATGACTGTCTCCCTGGTGGTTATTGTTTTTGAGCTTACTGGAGGCTTGGAATATATTGTT
  CCCCTTATGGCTGCAGTCATGACCAGTAAATGGGTTGGAGATGCCTTTGGCAGGGAAGGC
  ATTTATGAAGCACACATCCGATTAAATGGATACCCTTTCTTGGATGCAAAAGAAGAATTC
  ACTCATACCACCCTGGCTGCTGACGTTATGAGACCTCGAAGGAATGATCCTCCCTTAGCT
  GTCCTGACACAGGACAATATGACAGTGGATGATATAGAAAACATGATTAATGAAACCAGC
  TACAATGGATTTCCTGTCATAATGTCAAAAGAATCTCAGAGATTAGTGGGATTTGCCCTC
  AGAAGAGACCTGACAATTGCAATAGAAAGTGCCAGGAAAAAACAAGAAGGTATCGTTGGC
  AGTTCTCGGGTGTGTTTTGCACAGCACACCCCATCTCTTCCAGCAGAAAGTCCTCGGCCA
  TTGAAGCTTCGAAGCATTCTTGACATGAGCCCTTTTACAGTGACAGACCACACCCCAATG
  GAGATCGTGGTGGATATTTTCCGAAAGCTGGGACTGAGGCAGTGCCTTGTAACTCACAAT
  GGGCGCCTCCTTGGCATTATAACAAAAAAAGATATCCTCCGGCATATGGCCCAGACGGCA
  AACCAAGACCCCGCTTCAATAATGTTCAACTGAATCTCACAGATGAGGAGAGAGAAGAAA
  CGGAAGAGGAAGTTTATTTGTTGAATAGCACAACTCTTTAACCTGAGGGAGTCATCTACT
  TTTTTTTCCTCCTTTACAAAAAAAGAAAGGAAATATAAAAGCCGGGTTTTTGCAACATGG
  TTTGCAAATAATGCTGGTGGAATGGAGGAGTTGTTTGGGGAGGGAAAGGAGAGAGAAGGA
  AAGGAGTGAAGGGTATTTCCCGTCTAACAGAAAGCAGCGTATCAACTCCTATTGTTCTGC
  ACTGGATGCATTCAGCTGAGGATGTGCCTGATAGTGCAGGCTTGCGCCTCAACAGAGATG
  ACAGCAGAGTCCTCGAGCACCTGGCCTGTTGCTCCAACATTGCAAAGA
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_001829 unedited
  GCCCATTTAGTTATACGACTCACTATAGGCGGCCGCGNAATTCGCACGAGGCTTTCCCAG
  TGTTCTAGTTCGCCCGTGACCCGGGATAATGAGCAAGGAGGGTGTGGTGGGTTGAAAGCC
  ATCCTACTTTACTCCCGAGTTAGAGCATGGATTCAGTTTTAGTCTTAAGGGGGAAGTGAG
  ATTGGAGATTTTTATTTTTAATTTTGGGCAGAAGCAGGTTGACTCTAGGGATCTCCAGAG
  CGAGAGGATTTAACTTCATGTTGCTCCCGTGTTTGAAGGAGGACAATAAAAGTCCCACCG
  GGCAAAATTTTCGTAACCTCTGCGGTAGAAAACGTCAGGTATCTTTTAAATCGCGATAGT
  TTTCGCTGTGTCAGGCTTTCTTCGGTGGAGCTCCGAGGGTAGCTAGGTTCTAGGTTTGAA
  ACAGATGCAGAATCCAAAGGCAGCGCAAAAAACAGCCACCGATTTTGCTATGTCTCTGAG
  CTGCGAGATAATCAGACAGCTAAATGGAGTCTGAGCAGCTGTTCCATAGAGGCTACTATA
  GAAACAGCTACAACAGTATAACAAGTGCAAGTAGTGATGAGGAACTTTTAGATGGAGCAG
  GTGTTATTATGGACTTTCAAACATCTGAAGATGACCATTTATTAGATGGTGACACTGCAG
  TTGGAACTCATTATACCATGACAAATGGAGGCAGCATTAACAGTTCTACACATTTACTGG
  ATCTTTTGGATGACCCATTCCAGTGTTGGTACATATGATGATTTCCATACTATTGGATGG
  GTGCCAGAAAATGCTAAGACAGAGAAGGCATATACGGGTCAACAGCAAAAAGAAGAATCA
  GCCTGGGAAATGACACAAGTTNGTTTGATGCGCTGTCCAGAGGCCTCTANTAACACTACC
  AGATGGCCTCAGGGCCCTGGCCCGATAATGGACACTGCG
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_001829 unedited
  GGGTTAGCTTGNCCGCGGCCGCATTCTANGATCGAGTTTTTTTTTTTTTTTTTTGTATTT
  TATAAATTTTAATCCATAAAAATATAAATAAGAATGGTAAATTTTTAAGAAAAAAAATAT
  ACATTTAGTGCAAGTTATGAATTACATTTCTTCTTCAGTGTCAGCCATGAGACATCTTGA
  GAAAAGAAAAGAATGACTGATCTAATTTCAATTTTAAAAATTCCAGTGTTTTAACATGCA
  ATTAATACCAGATACAGCTAATCAGAATACTTCCTTCTCTTTACTGTCAAAGCAGCAGCA
  TTTTATTTAATTATGCTCAACAGCACTTGTATATCATAGTAGTAGTCTTAGAAATAGAAA
  ATTTGCATAAGACCCGGTCACATTATCCATAAAGTCTGAGAGGAAAACAAAAAAGAAAAC
  TTTATCCTTTAAAACCAAGCTTCATTGTTTTCGGTTTTGATCCACACGGCACACAATGAG
  AGATTTCGTTCTCGCTTCCTTCGAGACTTTACCTGTTTGATGAGCCTTTGTTGTGGTGCA
  TGTGTACTGTTCCAAGCATTTAGTGATGGTACAAACTACATGGTTAATGGCACAAGAAAC
  ACTCCCTTCTTGTGATGAAACCTCAGTAAAATCACAGTACATCTCAATATGAGAAAGGAC
  TGCTTCAAAAGGATACACCGATTTAAAGAGGCTTTGTTACAGCTCACTCNAGAGAGAGAG
  AGAGAGAGAGAGAGAGAAATAAAGGCTCTTCTGGAATGTAGAAAGGGTATGGCTTAACTA
  TACAATTCATGAATTAAACAGAACAGTTTTGATTAAAAAAAAAGGCCAGCACCTTGCAAG
  TTTATAGATGGCTCAATTCCAAAGTATCCCTTCTAGAATAGGGACCTGATATGTGTCTTT
  GCATGTTGAACTCACAGCCCAGTGCTCGAGGACTCTGCTGTCATTC
  
• Restriction Sites: NotI-NotI
• ACCN: NM_001829
• Insert Size: 4200 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001829.2, NP_001820.2
• RefSeq Size: 3982 bp
• RefSeq ORF: 2457 bp
• Locus ID: 1182
• UniProt ID: P51790
• Cytogenetics: 4q33
• Domains: CBS, voltage_CLC
• Protein Families: Druggable Genome, Ion Channels: Other, Transmembrane
• Gene Summary: This gene encodes a member of the voltage-gated chloride channel (ClC) family. The encoded protein is present in all cell types and localized in plasma membranes and in intracellular vesicles. It is a multi-pass membrane protein which contains a ClC domain and two additional C-terminal CBS (cystathionine beta-synthase) domains. The ClC domain catalyzes the selective flow of Cl- ions across cell membranes, and the CBS domain may have a regulatory function. This protein plays a role in both acidification and transmitter loading of GABAergic synaptic vesicles, and in smooth muscle cell activation and neointima formation. This protein is required for lysophosphatidic acid (LPA)-activated Cl- current activity and fibroblast-to-myofibroblast differentiation. The protein activity is regulated by Ca(2+)/calmodulin-dependent protein kinase II (CaMKII) in glioma cells. Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2011]
  Transcript Variant: This variant (b) represents the predominant transcript and encodes isoform b. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments.