RAD50 (NM_005732) Human Untagged Clone

CAT#: SC110122

RAD50 (untagged)-Human RAD50 homolog (S. cerevisiae) (RAD50)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: RAD50
• Synonyms: hRad50; NBSLD; RAD502
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >OriGene ORF within SC110122 sequence for NM_005732 edited (data generated by NextGen Sequencing)
  ATGTCCCGGATCGAAAAGATGAGCATTCTGGGCGTGCGGAGTTTTGGAATAGAGGACAAA
  GATAAGCAAATTATCACTTTCTTCAGCCCCCTTACAATTTTGGTTGGACCCAATGGGGCG
  GGAAAGACGACCATCATTGAATGTCTAAAATATATTTGTACTGGAGATTTCCCTCCTGGA
  ACCAAAGGAAATACATTTGTACACGATCCCAAGGTTGCTCAAGAAACAGATGTGAGAGCC
  CAGATTCGTCTGCAATTTCGTGATGTCAATGGAGAACTTATAGCTGTGCAAAGATCTATG
  GTGTGTACTCAGAAAAGCAAAAAGACAGAATTTAAAACTCTGGAAGGAGTCATTACTAGA
  ACAAAGCATGGTGAAAAGGTCAGTCTGAGCTCTAAGTGTGCAGAAATTGACCGAGAAATG
  ATCAGTTCTCTTGGGGTTTCCAAGGCTGTGCTAAATAATGTCATTTTCTGTCATCAAGAA
  GATTCTAATTGGCCTTTAAGTGAAGGAAAGGCTTTGAAGCAAAAGTTTGATGAGATTTTT
  TCAGCAACAAGATACATTAAAGCCTTAGAAACACTTCGGCAGGTACGTCAGACACAAGGT
  CAGAAAGTAAAAGAATATCAAATGGAACTAAAATATCTGAAGCAATATAAGGAAAAAGCT
  TGTGAGATTCGTGATCAGATTACAAGTAAGGAAGCCCAGTTAACATCTTCAAAGGAAATT
  GTCAAATCCTATGAGAATGAACTTGATCCATTGAAGAATCGTCTAAAAGAAATTGAACAT
  AATCTCTCTAAAATAATGAAACTTGACAATGAAATTAAAGCCTTGGATAGCCGAAAGAAG
  CAAATGGAGAAAGATAATAGTGAACTGGAAGAGAAAATGGAAAAGGTTTTTCAAGGGACT
  GATGAGCAACTAAATGACTTATATCACAATCACCAGAGAACAGTAAGGGAGAAAGAAAGG
  AAATTGGTAGACTGTCATCGTGAACTGGAAAAACTAAATAAAGAATCTAGGCTTCTCAAT
  CAGGAAAAATCAGAACTGCTTGTTGAACAGGGTCGTCTACAGCTGCAAGCAGATCGCCAT
  CAAGAACATATCCGAGCTAGAGATTCATTAATTCAGTCTTTGGCAACACAGCTAGAATTG
  GATGGCTTTGAGCGTGGACCATTCAGTGAAAGACAGATTAAAAATTTTCACAAACTTGTG
  AGAGAGAGACAAGAAGGGGAAGCAAAAACTGCCAACCAACTGATGAATGACTTTGCAGAA
  AAAGAGACTCTGAAACAAAAACAGATAGATGAGATAAGAGATAAGAAAACTGGACTGGGA
  AGAATAATTGAGTTAAAATCAGAAATCCTAAGTAAGAAGCAGAATGAGCTGAAAAATGTG
  AAGTATGAATTACAGCAGTTGGAAGGATCTTCAGACAGGATTCTTGAACTGGACCAGGAG
  CTCATAAAAGCTGAACGTGAGTTAAGCAAGGCTGAGAAAAACAGCAATGTAGAAACCTTA
  AAAATGGAAGTAATAAGTCTCCAAAATGAAAAAGCAGACTTAGACAGGACCCTGCGTAAA
  CTTGACCAGGAGATGGAGCAGTTAAACCATCATACAACAACACGTACCCAAATGGAGATG
  CTGACCAAAGACAAAGCTGACAAAGATGAACAAATCAGAAAAATAAAATCTAGGCACAGT
  GATGAATTAACCTCACTGTTGGGATATTTTCCCAACAAAAAACAGCTTGAAGACTGGCTA
  CATAGTAAATCAAAAGAAATTAATCAGACCAGGGACAGACTTGCCAAATTGAACAAGGAA
  CTAGCTTCATCTGAGCAGAATAAAAATCATATAAATAATGAACTAAAAAGAAAGGAAGAG
  CAGTTGTCCAGTTACGAAGACAAGCTGTTTGATGTTTGTGGTAGCCAGGATTTTGAAAGT
  GATTTAGACAGGCTTAAAGAGGAAATTGAAAAATCATCAAAACAGCGAGCCATGCTGGCT
  GGAGCCACAGCAGTTTACTCCCAGTTCATTACTCAGCTAACAGACGAAAACCAGTCATGT
  TGCCCCGTTTGTCAGAGAGTTTTTCAGACAGAGGCTGAGTTACAAGAAGTCATCAGTGAT
  TTGCAGTCTAAACTGCGACTTGCTCCAGATAAACTCAAGTCAACAGAATCAGAGCTAAAA
  AAAAAGGAAAAGCGGCGTGATGAAATGCTGGGACTTGTGCCCATGAGGCAAAGCATAATT
  GATTTGAAGGAGAAGGAAATACCAGAATTAAGAAACAAACTGCAGAATGTCAATAGAGAC
  ATACAGCGCCTAAAGAACGACATAGAAGAACAAGAAACACTCTTGGGTACAATAATGCCT
  GAAGAAGAAAGTGCCAAAGTATGCCTGACAGATGTTACAATTATGGAGAGGTTCCAGATG
  GAACTTAAAGATGTTGAAAGAAAAATTGCACAACAAGCAGCTAAGCTACAAGGAATAGAC
  TTAGATCGAACTGTCCAACAAGTCAACCAGGAGAAACAAGAGAAACAGCACAAGTTAGAC
  ACAGTTTCTAGTAAGATTGAATTGAATCGTAAGCTTATACAGGACCAGCAGGAACAGATT
  CAACATCTAAAAAGTACAACAAATGAGCTAAAATCTGAGAAACTTCAGATATCCACTAAT
  TTGCAACGTCGTCAGCAACTGGAGGAGCAGACTGTGGAATTATCCACTGAAGTTCAGTCT
  TTGTACAGAGAGATAAAGGATGCTAAAGAGCAGGTAAGCCCTTTGGAAACAACATTGGAA
  AAGTTCCAGCAAGAAAAAGAAGAATTAATCAACAAAAAAAATACAAGCAACAAAATAGCA
  CAGGATAAACTGAATGATATTAAAGAGAAGGTTAAAAATATTCATGGCTATATGAAAGAC
  ATTGAGAATTATATTCAAGATGGGAAAGACGACTATAAGAAGCAAAAAGAAACTGAACTT
  AATAAAGTAATAGCTCAACTAAGTGAATGCGAGAAACACAAAGAAAAGATAAATGAAGAT
  ATGAGACTCATGAGACAAGATATTGATACACAGAAGATACAAGAAAGGTGGCTACAAGAT
  AACCTTACTTTAAGAAAAAGAAATGAGGAACTAAAAGAAGTTGAAGAAGAAAGAAAACAA
  CATTTGAAGGAAATGGGTCAAATGCAGGTTTTGCAAATGAAAAGTGAACATCAGAAGTTG
  GAAGAGAACATAGACAATATAAAAAGAAATCATAATTTGGCATTAGGGCGACAGAAAGGT
  TATGAAGAAGAAATTATTCATTTTAAGAAAGAACTTCGAGAACCACAATTTCGGGATGCT
  GAGGAAAAGTATAGAGAAATGATGATTGTTATGAGGACAACAGAACTTGTGAACAAGGAT
  CTGGATATTTATTATAAGACTCTTGACCAAGCAATAATGAAATTTCACAGTATGAAAATG
  GAAGAAATCAATAAAATTATACGTGACCTGTGGCGAAGTACCTATCGTGGACAAGATATT
  GAATACATAGAAATACGGTCTGATGCCGATGAAAATGTATCAGCTTCTGATAAAAGGCGG
  AATTATAACTACCGAGTGGTGATGCTGAAGGGAGACACAGCCTTGGATATGCGAGGACGA
  TGCAGTGCTGGACAAAAGGTATTAGCCTCACTCATCATTCGCCTGGCCCTGGCTGAAACG
  TTCTGCCTCAACTGTGGCATCATTGCCTTGGATGAGCCAACAACAAATCTTGACCGAGAA
  AACATTGAATCTCTTGCACATGCTCTGGTTGAGATAATAAAAAGTCGCTCACAGCAGCGT
  AACTTCCAGCTTCTGGTAATCACTCATGATGAAGATTTTGTGGAGCTTTTAGGACGTTCT
  GAATATGTGGAGAAATTCTACAGGATTAAAAAGAACATCGATCAGTGCTCAGAGATTGTG
  AAATGCAGTGTTAGCTCCCTGGGATTCAATGTTCATTAA
  
  Clone variation with respect to NM_005732.3
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_005732 unedited
  GTACCATTTGTATACGACTCCTATAGGCGGCCGCGNAATTCGCACGAGGGTCGCATTGTG
  GCTACGGCTTTGCGTCCCCGGCGGGCAGCCCCAGGCTGGTCCCCGCCTCCGCTCTCCCCA
  CCGGCGGGGAAAGCAGCTGGTGTGGGAGGAAAGGCTCCATCCCCCGCCCCCTCTCTCCCG
  CTGTTGGCTGGCAGGATCTTTTGGCAGTCCTGTGGCCTCGCTCCCCGCCCGGATCCTCCT
  GACCCTGAGATTCGCGGGTCTCACGTCCCGTGCACGCCTTGCTTCGGCCTCAGTTAAGCC
  TTTGTGGACTCCAGGTCCCTGGTGAGATTAGAAACGTTTGCAAACATGTCCCGGATCGAA
  AAGATGAGCATTCTGGGCGTGCGGAGTTTTGGAATAGAGGACAAAGATAAGCAAATTATC
  ACTTTCTTCAGCCCCCTTACAATTTTGGTTGGACCCAATGGGGCGGGAAAGACGACCATC
  ATTGAATGTCTAAAATATATTTGTACTGGAGATTTCCCTCCTGGAACCAAAGGAAATACA
  TTTGTACACGATCCCAAGGTTGCTCAAGAAACAGATGTGAGAGCCCAGATTCGTCTGCAA
  TTTCGTGATGTCAATGGAGAACTTATAGCTGTGCAAAGATCTATGGTGTGTACTCAGAAA
  AGCANAAAGACAGAATTTAAAACTCTGGAAGGAGTCATTACTAGAACAAAGCATGGTGAA
  AAGGTCAGTCTGAGCTCTAAGTGTGCAGAAATTGACCGAGNAATGATCAGTTCTCTTGGG
  GTTTCCCAGGCTGTNGCTAATAATGTCATTNTCTGTCATCAAGAAGAATTCTATTGGCCT
  TTAAGTGAAGGAAAGGGCTTTGAGCAAAGTTTGATGAGATTTTTTCAGCACAGAAACATT
  AAGCCTTAAAACACTNCGCAGGTACTCAGACCAGN
  
  3' Read Nucleotide Sequence

  >OriGene 3' read for NM_005732 unedited
  TTGCTATGGCCCGGCGGCCGCAATCTAAGATCGCGTTTTTTTTTTTTTTTTTTTTTTTTT
  TTTTTTTTTTTCCTTTGAAAAAAATATTTTTTTATTGACTAAGTGGATATGAAAAATAAG
  TTTCTTATTATACACTTTCTGAGGACCTACATTTTTATGGCATTAAAATCTTGGATATTT
  TTAAGGAACATTGAATCCCAGGGAGCTAACACTGCATTTAACAATCTTTGACCACTGATC
  GATGTTTTTTTTAATCCTGAAAAATTTCTCCACATATTCAAAACGTCCTAAAAGCTCCAC
  AAAATCTTCATCATGAGGGATTACCAAAAGCTGGAAGTTACGCTGCTGGGAGCGACTTTT
  TATTATCTCAACCAAAGCATGTGCAAAAAATTCAAGGTTTTCTCGGCCAAAATTTGTTGT
  GGGCTCATCCAAGGCAATGATGCCACAGTTGAGGCAAAACGTTTCAGCCAGGGCCAGGCG
  AATGATGAGTGAGGCTAATACCTTTTGTCCAGCACTGCATCGTCCTCGCATATCCAAGGC
  TGTGTCTCCCTTCAGCATCACCACTCGGTAGTTATAATTCCGCCTTTTATCAGAAGCTGA
  TACATTTTCATCGGCATCAGACCGTATTTCTATGTATTCAATATCTTTGCCCCGATAGGT
  ACTTTCGCCCCAGTTCACGTAATAATTTAATGGATTCCTTCCATTTTCATACTGGGGAAA
  ATTCATTATTGCCTGGGCCAAGAGCCTTAATATAAATACCAGATCCTTGTCACAAAGTTC
  TGGTGGCCCTCATACAACATCATTTCTTTATACTTTCCTCAGATCCGAAAATGTGTCCTT
  CGAAGTTTTTTCTAAAAGAAAAATTTTTCTTCACCCCTTCTGCCGCCTATGCAAATAAGG
  AATCTTTTTAATGGCGTATGGTCCTTCCAACTCGGAGGCCACTTTCATTGCAACCCGGCT
  T
  
• Restriction Sites: NotI-NotI
• ACCN: NM_005732
• Insert Size: 5000 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005732.2, NP_005723.2
• RefSeq Size: 5891 bp
• RefSeq ORF: 3939 bp
• Locus ID: 10111
• UniProt ID: Q92878
• Cytogenetics: 5q31.1
• Domains: Rad50_zn_hook
• Protein Families: Druggable Genome
• Protein Pathways: Homologous recombination, Non-homologous end-joining
• Gene Summary: The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Rad50, a protein involved in DNA double-strand break repair. This protein forms a complex with MRE11 and NBS1. The protein complex binds to DNA and displays numerous enzymatic activities that are required for nonhomologous joining of DNA ends. This protein, cooperating with its partners, is important for DNA double-strand break repair, cell cycle checkpoint activation, telomere maintenance, and meiotic recombination. Knockout studies of the mouse homolog suggest this gene is essential for cell growth and viability. Mutations in this gene are the cause of Nijmegen breakage syndrome-like disorder.[provided by RefSeq, Apr 2010]
  Transcript Variant: This variant (1) contains an internal region absent in variant 2. The resulting protein is 139 aa longer at its N-terminus, as compared to isoform 2.