RAD50 (NM_005732) Human Tagged ORF Clone

CAT#: RG211751

RAD50 (tGFP-tagged) - Human RAD50 homolog (S. cerevisiae) (RAD50)

Product Images

Circular map for RG211751

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: RAD50
• Synonyms: hRad50; NBSLD; RAD502
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG211751 representing NM_005732
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCCGGATCGAAAAGATGAGCATTCTGGGCGTGCGGAGTTTTGGAATAGAGGACAAAGATAAGCAAA
  TTATCACTTTCTTCAGCCCCCTTACAATTTTGGTTGGACCCAATGGGGCGGGAAAGACGACCATCATTGA
  ATGTCTAAAATATATTTGTACTGGAGATTTCCCTCCTGGAACCAAAGGAAATACATTTGTACACGATCCC
  AAGGTTGCTCAAGAAACAGATGTGAGAGCCCAGATTCGTCTGCAATTTCGTGATGTCAATGGAGAACTTA
  TAGCTGTGCAAAGATCTATGGTGTGTACTCAGAAAAGCAAAAAGACAGAATTTAAAACTCTGGAAGGAGT
  CATTACTAGAACAAAGCATGGTGAAAAGGTCAGTCTGAGCTCTAAGTGTGCAGAAATTGACCGAGAAATG
  ATCAGTTCTCTTGGGGTTTCCAAGGCTGTGCTAAATAATGTCATTTTCTGTCATCAAGAAGATTCTAATT
  GGCCTTTAAGTGAAGGAAAGGCTTTGAAGCAAAAGTTTGATGAGATTTTTTCAGCAACAAGATACATTAA
  AGCCTTAGAAACACTTCGGCAGGTACGTCAGACACAAGGTCAGAAAGTAAAAGAATATCAAATGGAACTA
  AAATATCTGAAGCAATATAAGGAAAAAGCTTGTGAGATTCGTGATCAGATTACAAGTAAGGAAGCCCAGT
  TAACATCTTCAAAGGAAATTGTCAAATCCTATGAGAATGAACTTGATCCATTGAAGAATCGTCTAAAAGA
  AATTGAACATAATCTCTCTAAAATAATGAAACTTGACAATGAAATTAAAGCCTTGGATAGCCGAAAGAAG
  CAAATGGAGAAAGATAATAGTGAACTGGAAGAGAAAATGGAAAAGGTTTTTCAAGGGACTGATGAGCAAC
  TAAATGACTTATATCACAATCACCAGAGAACAGTAAGGGAGAAAGAAAGGAAATTGGTAGACTGTCATCG
  TGAACTGGAAAAACTAAATAAAGAATCTAGGCTTCTCAATCAGGAAAAATCAGAACTGCTTGTTGAACAG
  GGTCGTCTACAGCTGCAAGCAGATCGCCATCAAGAACATATCCGAGCTAGAGATTCATTAATTCAGTCTT
  TGGCAACACAGCTAGAATTGGATGGCTTTGAGCGTGGACCATTCAGTGAAAGACAGATTAAAAATTTTCA
  CAAACTTGTGAGAGAGAGACAAGAAGGGGAAGCAAAAACTGCCAACCAACTGATGAATGACTTTGCAGAA
  AAAGAGACTCTGAAACAAAAACAGATAGATGAGATAAGAGATAAGAAAACTGGACTGGGAAGAATAATTG
  AGTTAAAATCAGAAATCCTAAGTAAGAAGCAGAATGAGCTGAAAAATGTGAAGTATGAATTACAGCAGTT
  GGAAGGATCTTCAGACAGGATTCTTGAACTGGACCAGGAGCTCATAAAAGCTGAACGTGAGTTAAGCAAG
  GCTGAGAAAAACAGCAATGTAGAAACCTTAAAAATGGAAGTAATAAGTCTCCAAAATGAAAAAGCAGACT
  TAGACAGGACCCTGCGTAAACTTGACCAGGAGATGGAGCAGTTAAACCATCATACAACAACACGTACCCA
  AATGGAGATGCTGACCAAAGACAAAGCTGACAAAGATGAACAAATCAGAAAAATAAAATCTAGGCACAGT
  GATGAATTAACCTCACTGTTGGGATATTTTCCCAACAAAAAACAGCTTGAAGACTGGCTACATAGTAAAT
  CAAAAGAAATTAATCAGACCAGGGACAGACTTGCCAAATTGAACAAGGAACTAGCTTCATCTGAGCAGAA
  TAAAAATCATATAAATAATGAACTAAAAAGAAAGGAAGAGCAGTTGTCCAGTTACGAAGACAAGCTGTTT
  GATGTTTGTGGTAGCCAGGATTTTGAAAGTGATTTAGACAGGCTTAAAGAGGAAATTGAAAAATCATCAA
  AACAGCGAGCCATGCTGGCTGGAGCCACAGCAGTTTACTCCCAGTTCATTACTCAGCTAACAGACGAAAA
  CCAGTCATGTTGCCCCGTTTGTCAGAGAGTTTTTCAGACAGAGGCTGAGTTACAAGAAGTCATCAGTGAT
  TTGCAGTCTAAACTGCGACTTGCTCCAGATAAACTCAAGTCAACAGAATCAGAGCTAAAAAAAAAGGAAA
  AGCGGCGTGATGAAATGCTGGGACTTGTGCCCATGAGGCAAAGCATAATTGATTTGAAGGAGAAGGAAAT
  ACCAGAATTAAGAAACAAACTGCAGAATGTCAATAGAGACATACAGCGCCTAAAGAACGACATAGAAGAA
  CAAGAAACACTCTTGGGTACAATAATGCCTGAAGAAGAAAGTGCCAAAGTATGCCTGACAGATGTTACAA
  TTATGGAGAGGTTCCAGATGGAACTTAAAGATGTTGAAAGAAAAATTGCACAACAAGCAGCTAAGCTACA
  AGGAATAGACTTAGATCGAACTGTCCAACAAGTCAACCAGGAGAAACAAGAGAAACAGCACAAGTTAGAC
  ACAGTTTCTAGTAAGATTGAATTGAATCGTAAGCTTATACAGGACCAGCAGGAACAGATTCAACATCTAA
  AAAGTACAACAAATGAGCTAAAATCTGAGAAACTTCAGATATCCACTAATTTGCAACGTCGTCAGCAACT
  GGAGGAGCAGACTGTGGAATTATCCACTGAAGTTCAGTCTTTGTACAGAGAGATAAAGGATGCTAAAGAG
  CAGGTAAGCCCTTTGGAAACAACATTGGAAAAGTTCCAGCAAGAAAAAGAAGAATTAATCAACAAAAAAA
  ATACAAGCAACAAAATAGCACAGGATAAACTGAATGATATTAAAGAGAAGGTTAAAAATATTCATGGCTA
  TATGAAAGACATTGAGAATTATATTCAAGATGGGAAAGACGACTATAAGAAGCAAAAAGAAACTGAACTT
  AATAAAGTAATAGCTCAACTAAGTGAATGCGAGAAACACAAAGAAAAGATAAATGAAGATATGAGACTCA
  TGAGACAAGATATTGATACACAGAAGATACAAGAAAGGTGGCTACAAGATAACCTTACTTTAAGAAAAAG
  AAATGAGGAACTAAAAGAAGTTGAAGAAGAAAGAAAACAACATTTGAAGGAAATGGGTCAAATGCAGGTT
  TTGCAAATGAAAAGTGAACATCAGAAGTTGGAAGAGAACATAGACAATATAAAAAGAAATCATAATTTGG
  CATTAGGGCGACAGAAAGGTTATGAAGAAGAAATTATTCATTTTAAGAAAGAACTTCGAGAACCACAATT
  TCGGGATGCTGAGGAAAAGTATAGAGAAATGATGATTGTTATGAGGACAACAGAACTTGTGAACAAGGAT
  CTGGATATTTATTATAAGACTCTTGACCAAGCAATAATGAAATTTCACAGTATGAAAATGGAAGAAATCA
  ATAAAATTATACGTGACCTGTGGCGAAGTACCTATCGTGGACAAGATATTGAATACATAGAAATACGGTC
  TGATGCCGATGAAAATGTATCAGCTTCTGATAAAAGGCGGAATTATAACTACCGAGTGGTGATGCTGAAG
  GGAGACACAGCCTTGGATATGCGAGGACGATGCAGTGCTGGACAAAAGGTATTAGCCTCACTCATCATTC
  GCCTGGCCCTGGCTGAAACGTTCTGCCTCAACTGTGGCATCATTGCCTTGGATGAGCCAACAACAAATCT
  TGACCGAGAAAACATTGAATCTCTTGCACATGCTCTGGTTGAGATAATAAAAAGTCGCTCACAGCAGCGT
  AACTTCCAGCTTCTGGTAATCACTCATGATGAAGATTTTGTGGAGCTTTTAGGACGTTCTGAATATGTGG
  AGAAATTCTACAGGATTAAAAAGAACATCGATCAGTGCTCAGAGATTGTGAAATGCAGTGTTAGCTCCCT
  GGGATTCAATGTTCAT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG211751 representing NM_005732
  Red=Cloning site Green=Tags(s)
  
  MSRIEKMSILGVRSFGIEDKDKQIITFFSPLTILVGPNGAGKTTIIECLKYICTGDFPPGTKGNTFVHDP
  KVAQETDVRAQIRLQFRDVNGELIAVQRSMVCTQKSKKTEFKTLEGVITRTKHGEKVSLSSKCAEIDREM
  ISSLGVSKAVLNNVIFCHQEDSNWPLSEGKALKQKFDEIFSATRYIKALETLRQVRQTQGQKVKEYQMEL
  KYLKQYKEKACEIRDQITSKEAQLTSSKEIVKSYENELDPLKNRLKEIEHNLSKIMKLDNEIKALDSRKK
  QMEKDNSELEEKMEKVFQGTDEQLNDLYHNHQRTVREKERKLVDCHRELEKLNKESRLLNQEKSELLVEQ
  GRLQLQADRHQEHIRARDSLIQSLATQLELDGFERGPFSERQIKNFHKLVRERQEGEAKTANQLMNDFAE
  KETLKQKQIDEIRDKKTGLGRIIELKSEILSKKQNELKNVKYELQQLEGSSDRILELDQELIKAERELSK
  AEKNSNVETLKMEVISLQNEKADLDRTLRKLDQEMEQLNHHTTTRTQMEMLTKDKADKDEQIRKIKSRHS
  DELTSLLGYFPNKKQLEDWLHSKSKEINQTRDRLAKLNKELASSEQNKNHINNELKRKEEQLSSYEDKLF
  DVCGSQDFESDLDRLKEEIEKSSKQRAMLAGATAVYSQFITQLTDENQSCCPVCQRVFQTEAELQEVISD
  LQSKLRLAPDKLKSTESELKKKEKRRDEMLGLVPMRQSIIDLKEKEIPELRNKLQNVNRDIQRLKNDIEE
  QETLLGTIMPEEESAKVCLTDVTIMERFQMELKDVERKIAQQAAKLQGIDLDRTVQQVNQEKQEKQHKLD
  TVSSKIELNRKLIQDQQEQIQHLKSTTNELKSEKLQISTNLQRRQQLEEQTVELSTEVQSLYREIKDAKE
  QVSPLETTLEKFQQEKEELINKKNTSNKIAQDKLNDIKEKVKNIHGYMKDIENYIQDGKDDYKKQKETEL
  NKVIAQLSECEKHKEKINEDMRLMRQDIDTQKIQERWLQDNLTLRKRNEELKEVEEERKQHLKEMGQMQV
  LQMKSEHQKLEENIDNIKRNHNLALGRQKGYEEEIIHFKKELREPQFRDAEEKYREMMIVMRTTELVNKD
  LDIYYKTLDQAIMKFHSMKMEEINKIIRDLWRSTYRGQDIEYIEIRSDADENVSASDKRRNYNYRVVMLK
  GDTALDMRGRCSAGQKVLASLIIRLALAETFCLNCGIIALDEPTTNLDRENIESLAHALVEIIKSRSQQR
  NFQLLVITHDEDFVELLGRSEYVEKFYRIKKNIDQCSEIVKCSVSSLGFNVH
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_005732
• ORF Size: 3936 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_005732.4
• RefSeq Size: 6597 bp
• RefSeq ORF: 3939 bp
• Locus ID: 10111
• UniProt ID: Q92878
• Cytogenetics: 5q31.1
• Domains: Rad50_zn_hook
• Protein Families: Druggable Genome
• Protein Pathways: Homologous recombination, Non-homologous end-joining
• Gene Summary: The protein encoded by this gene is highly similar to Saccharomyces cerevisiae Rad50, a protein involved in DNA double-strand break repair. This protein forms a complex with MRE11 and NBS1. The protein complex binds to DNA and displays numerous enzymatic activities that are required for nonhomologous joining of DNA ends. This protein, cooperating with its partners, is important for DNA double-strand break repair, cell cycle checkpoint activation, telomere maintenance, and meiotic recombination. Knockout studies of the mouse homolog suggest this gene is essential for cell growth and viability. Mutations in this gene are the cause of Nijmegen breakage syndrome-like disorder.[provided by RefSeq, Apr 2010]