EFHA1 (MICU2) (NM_152726) Human Untagged Clone

CAT#: SC101057

EFHA1 (untagged)-Human EF-hand domain family, member A1 (EFHA1)

Specifications

Product Data

• Type: Human Untagged Clone
• Tag: Tag Free
• Symbol: MICU2
• Synonyms: 1110008L20Rik; EFHA1
• Vector: pCMV6-XL4
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: None
• Sequence Data:
  Fully Sequenced ORF

  >NCBI ORF sequence for NM_152726, the custom clone sequence may differ by one or more nucleotides
  
  
  ATGGCGGCGGCTGCGGGTAGCTGCGCGCGGGTGGCGGCCTGGGGCGGAAAACTGCGACGGGGGCTCGCTG
  TCAGCCGACAGGCTGTGCGGAGTCCCGGCCCCTTGGCAGCGGCAGTGGCCGGCGCGGCCCTGGCAGGAGC
  AGGAGCGGCCTGGCACCACAGCCGCGTCAGTGTTGCGGCGCGGGATGGCAGTTTTACAGTCTCCGCACAG
  AAAAATGTTGAACATGGAATAATATATATTGGGAAACCGTCTCTTCGTAAGCAGCGCTTCATGCAGTTTT
  CTTCACTCGAACATGAAGGAGAATATTATATGACACCACGAGACTTCCTCTTCTCAGTGATGTTTGAGCA
  AATGGAACGTAAAACTTCAGTCAAGAAGCTGACAAAAAAGGACATCGAGGATACACTGTCAGGGATCCAA
  ACAGCTGGCTGTGGATCAACTTTTTTCAGAGACCTTGGCGATAAAGGGCTAATTTCATATACCGAGTATC
  TTTTCTTGCTTACAATCCTCACTAAACCCCATTCTGGATTTCATGTTGCTTTTAAAATGCTGGATACAGA
  TGGTAATGAGATGATTGAAAAAAGGGAATTTTTTAAGCTGCAGAAGATCATAAGTAAACAAGATGACTTG
  ATGACAGTGAAAACTAATGAAACTGGATATCAGGAAGCAATAGTGAAAGAACCTGAAATTAACACAACTC
  TTCAGATGCGTTTCTTTGGAAAAAGAGGACAAAGAAAACTTCATTATAAAGAATTTCGAAGATTTATGGA
  AAATTTACAAACAGAGATTCAAGAAATGGAATTCCTTCAGTTTTCTAAAGGTTTGAGTTTCATGAGAAAA
  GAAGACTTTGCAGAGTGGCTACTTTTTTTCACTAACACTGAAAATAAAGATATTTATTGGAAAAATGTGA
  GAGAGAAGTTGTCAGCAGGAGAGAGCATTAGTTTGGATGAATTCAAGTCATTTTGCCATTTTACAACCCA
  CTTGGAAGACTTTGCTATTGCCATGCAGATGTTCAGTTTAGCTCATCGTCCTGTCAGACTAGCGGAGTTT
  AAGAGAGCTGTGAAAGTAGCAACAGGACAAGAACTCTCAAACAATATTTTGGACACTGTCTTTAAGATCT
  TTGATTTGGATGGTGATGAATGTCTTAGTCATGAAGAGTTTCTTGGGGTGTTAAAAAACAGAATGCATCG
  AGGTTTATGGGTACCACAACATCAGAGTATACAAGAATACTGGAAGTGTGTGAAGAAAGAAAGCATTAAA
  GGAGTAAAAGAAGTCTGGAAACAAGCTGGAAAAGGTCTTTTTTAA
  
  
  5' Read Nucleotide Sequence

  >OriGene 5' read for NM_152726 unedited
  AGAAATTTGTAATACGACTCACTATAGGGCGGCNCGCGAATCGGCACGAGGCTTCCGCAA
  AGAGGCGGCGGCTGCGGGTAGCTGCGCGCGGGTGGCGGCCTGGGGCGGAAAACTGCGACG
  GGGGCTCGCTGTCAGCCGACAGGCTGTGCGGAGTCCCGGCCCCTTGGCAGCGGCAGTGGC
  CGGCGCGGCCCTGGCAGGAGCAGGAGCGGCCTGGCACCACAGCCGCGTCAGTGTTGCGGC
  GCGGGATGGCAGTTTTACAGTCTCCGCACAGAAAAATGTTGAACATGGAATAATATATAT
  TGGGAAACCGTCTCTTCGTAAGCAGCGCTTCATGCAGTTTTCTTCACTCGAACATGAAGG
  AGAATATTATATGACACCACGAGACTTCCTCTTCTCAGTGATGTTTGAGCAAATGGAACG
  TAAAACTTCAGTCAAGAAGCTGACAAAAAAGGACATCGAGGATACACTGTCAGGGATCCA
  AACAGCTGGCTGTGGATCAACTTTTTTCAGAGACCTTGGCGATAAAGGGCTAATTTCATA
  TACCGAGTATCTTTTCTTGCTTACAATCCTCACTAAACCCCATTCTGGATTTCATGTTGC
  TTTTAAAATGCTGGATACAGATGGTAATGAGATGATTGAAAAAAGGGAATTTTTTAAGGT
  AAGTGGACGCTAATTATTTTAGGTTTATCATAAAATACCTGGATGTTTGTGTGATAATTN
  TACATTTCCATTAAAATCAAAATTGTATTCATTGTTTACATATGAACTATCANGAAATTA
  TTTGGACATTCAAAATATTAAATTTTCTATATACAACCATAGGACACCAGNTAGTGAACC
  AGAGCATAGATTCCAANAGAGAAGTAGCAGAGCATCTGTTTTTTAGGGTACATTTTATGA
  TTTAGCAATATATGGTAGAAGAGTTCATAGAATATATTTTAAGAATTGTNGTGAATGCTA
  AATATACAAN
  
• Restriction Sites: NotI-NotI
• ACCN: NM_152726
• Insert Size: 5500 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_152726.1, NP_689939.1
• RefSeq Size: 1891 bp
• RefSeq ORF: 1305 bp
• Locus ID: 221154
• UniProt ID: Q8IYU8
• Cytogenetics: 13q12.11
• Domains: EFh
• Gene Summary: Key regulator of mitochondrial calcium uniporter (MCU) required to limit calcium uptake by MCU when cytoplasmic calcium is low (PubMed:24503055, PubMed:24560927, PubMed:26903221). MICU1 and MICU2 form a disulfide-linked heterodimer that stimulate and inhibit MCU activity, depending on the concentration of calcium (PubMed:24560927). MICU2 acts as a gatekeeper of MCU that senses calcium level via its EF-hand domains: prevents channel opening at resting calcium, avoiding energy dissipation and cell-death triggering (PubMed:24560927).[UniProtKB/Swiss-Prot Function]