EFHA1 (MICU2) (NM_152726) Human Tagged ORF Clone

CAT#: RG205455

EFHA1 (tGFP-tagged) - Human EF-hand domain family, member A1 (EFHA1)

Product Images

Circular map for RG205455

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: TurboGFP
• Symbol: EFHA1
• Synonyms: 1110008L20Rik; EFHA1
• Vector: pCMV6-AC-GFP
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RG205455 representing NM_152726
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCGGCTGCGGGTAGCTGCGCGCGAGTGGCGGCCTGGGGCGGAAAACTGCGACGGGGGCTCGCTG
  TCAGCCGACAGGCTGTGCGGAGTCCCGGCCCCTTGGCAGCGGCAGTGGCCGGCGCGGCCCTGGCAGGAGC
  AGGAGCGGCCTGGCACCACAGCCGCGTCAGTGTTGCGGCGCGGGATGGCAGTTTTACAGTCTCCGCACAG
  AAAAATGTTGAACATGGAATAATATATATTGGGAAACCGTCTCTTCGTAAGCAGCGCTTCATGCAGTTTT
  CTTCACTCGAACATGAAGGAGAATATTATATGACACCACGAGACTTCCTCTTCTCAGTGATGTTTGAGCA
  AATGGAACGTAAAACTTCAGTCAAGAAGCTGACAAAAAAGGACATCGAGGATACACTGTCAGGGATCCAA
  ACAGCTGGCTGTGGATCAACTTTTTTCAGAGACCTTGGCGATAAAGGGCTAATTTCATATACCGAGTATC
  TTTTCTTGCTTACAATCCTCACTAAACCCCATTCTGGATTTCATGTTGCTTTTAAAATGCTGGATACAGA
  TGGTAATGAGATGATTGAAAAAAGGGAATTTTTTAAGCTGCAGAAGATCATAAATAAACAAGATGACTTG
  ATGACAGTGAAAACTAATGAAACTGGATATCAGGAAGCAATAGTGAAAGAACCTGAAATTAACACAACTC
  TTCAGATGCGTTTCTTTGGAAAAAGAGGACAAAGAAAACTTCATTATAAAGAATTTCGAAGATTTATGGA
  AAATTTACTAACAGAGATTCAAGAAATGGAATTCCTTCAGTTTTCTAAAGGTTTGAGTTTCATGAGAAAA
  GAAGACTTTGCAGAGTGGCTACTTTTTTTCACTAACACTGAAAATAAAGATATTTATTGGAAAAATGTGA
  GAGAGAAGTTGTCAGCAGGAGAGAGCATTAGTTTGGATGAATTCAAGTCATTTTGCCATTTTACAACCCA
  CTTGGAAGACTTTGCTATTGCCATGCAGATGTTCAGTTTAGCTCATCGTCCTGTCAGACTAGCGGAGTTT
  AAGAGAGCTGTGAAAGTAGCAACAGGACAAGAACTCTCAAACAATATTTTGGACACTGTCTTTAAGATCT
  TTGATTTGGATGGTGATGAATGTCTTAGTCATGAAGAGTTTCTTGGGGTGTTAAAAAACAGAATGCATCG
  AGGTTTATGGGTACCACAACATCAGAGTATACAAGAATACTGGAAGTGTGTGAAGAAAGAAAGCATTAAA
  GGAGTAAAAGAAGTCTGGAAACAAGCTGGAAAAGGTCTTTTT
  
  ACGCGTACGCGGCCGCTCGAG - GFP Tag - GTTTAA
  Protein Sequence

  >RG205455 representing NM_152726
  Red=Cloning site Green=Tags(s)
  
  MAAAAGSCARVAAWGGKLRRGLAVSRQAVRSPGPLAAAVAGAALAGAGAAWHHSRVSVAARDGSFTVSAQ
  KNVEHGIIYIGKPSLRKQRFMQFSSLEHEGEYYMTPRDFLFSVMFEQMERKTSVKKLTKKDIEDTLSGIQ
  TAGCGSTFFRDLGDKGLISYTEYLFLLTILTKPHSGFHVAFKMLDTDGNEMIEKREFFKLQKIINKQDDL
  MTVKTNETGYQEAIVKEPEINTTLQMRFFGKRGQRKLHYKEFRRFMENLLTEIQEMEFLQFSKGLSFMRK
  EDFAEWLLFFTNTENKDIYWKNVREKLSAGESISLDEFKSFCHFTTHLEDFAIAMQMFSLAHRPVRLAEF
  KRAVKVATGQELSNNILDTVFKIFDLDGDECLSHEEFLGVLKNRMHRGLWVPQHQSIQEYWKCVKKESIK
  GVKEVWKQAGKGLF
  
  TRTRPLE - GFP Tag - V
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_152726
• ORF Size: 1302 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_152726.1, NP_689939.1
• RefSeq Size: 1891 bp
• RefSeq ORF: 1305 bp
• Locus ID: 221154
• UniProt ID: Q8IYU8
• Cytogenetics: 13q12.11
• Domains: EFh
• Gene Summary: Key regulator of mitochondrial calcium uniporter (MCU) required to limit calcium uptake by MCU when cytoplasmic calcium is low (PubMed:24503055, PubMed:24560927, PubMed:26903221). MICU1 and MICU2 form a disulfide-linked heterodimer that stimulate and inhibit MCU activity, depending on the concentration of calcium (PubMed:24560927). MICU2 acts as a gatekeeper of MCU that senses calcium level via its EF-hand domains: prevents channel opening at resting calcium, avoiding energy dissipation and cell-death triggering (PubMed:24560927).[UniProtKB/Swiss-Prot Function]