Myh11 (NM_001170600) Rat Tagged ORF Clone

CAT#: RR217716

Myh11 (myc-DDK-tagged) - Rat myosin, heavy chain 11, smooth muscle (Myh11)

Product Images

Circular map for RR217716

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Myh11
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR217716 representing NM_001170600
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCACAGAAAGGGCAGCTCAGTGATGATGAGAAGTTCCTCTTTGTGGATAAAAACTTCATAAACAGCC
  CAATGGCCCAGGCCGACTGGGTAGCCAAGAAGCTAGTATGGGTCCCTTCAGAGAAGCAGGGCTTCGAAGC
  AGCCAGCATCAAGGAGGAGAAGGGCGATGAGGTGGTTGTAGAGCTGGTGGAAAACGGAAAGAAGGTCACA
  GTGGGCAAAGATGACATCCAAAAAATGAACCCGCCCAAGTTCTCCAAGGTGGAAGACATGGCCGAGCTGA
  CATGCCTCAATGAGGCTTCTGTGCTGCACAACCTGAGGGAGCGGTACTTCTCAGGCCTCATCTACACCTA
  CTCGGGCCTCTTCTGTGTGGTGGTCAACCCCTACAAGCACCTACCCATCTACTCAGAAAAGATTGTGGAC
  ATGTACAAGGGCAAGAAGAGGCATGAGATGCCGCCTCACATCTATGCCATTGCTGATACGGCCTACAGAA
  GCATGCTACAAGATCGTGAAGACCAGTCCATTCTGTGCACAGGTGAGTCTGGAGCTGGAAAGACAGAGAA
  CACAAAGAAAGTCATACAGTATTTGGCTGTGGTGGCATCCTCCCACAAAGGCAAGAAAGACAGCAGCATC
  ACGGGGGAGCTGGAAAAGCAGCTTCTACAGGCAAACCCAATCCTGGAGGCTTTCGGCAATGCAAAAACTG
  TCAAGAATGACAACTCCTCCCGCTTTGGCAAGTTCATTCGCATCAACTTTGATGTCACTGGTTACATTGT
  GGGTGCCAATATCGAAACATATCTTCTGGAAAAGTCTCGGGCTATTCGACAGGCTAGGGACGAGAGAACG
  TTCCACATCTTCTACTATCTGATCGCTGGAGCCAAGGAGAAGATGAGAAATGACCTACTTTTGGAGAGCT
  TCAACAGCTACACATTTTTATCCAATGGCTTTGTGCCCATCCCAGCTGCACAAGATGATGAGATGTTCCA
  GGAGACACTAGAGGCCATGTCCATCATGGGCTTCAGTGAAGAGGAACAGCTAGCCATCTTGAAGGTAGTA
  TCATCCGTCCTTCAGCTTGGAAACATTGTCTTCAAGAAGGAGCGAAACACAGACCAGGCATCCATGCCTG
  ATAACACAGCTGCTCAGAAAGTTTGCCACCTTGTGGGGATTAATGTGACAGATTTCACCAGAGCCATCCT
  GACCCCACGTATAAAAGTTGGACGGGATGTGGTGCAGAAAGCTCAGACCAAAGAACAGGCTGACTTTGCC
  ATTGAGGCCTTAGCCAAGGCCACCTATGAGCGCCTTTTCCGATGGATTCTCAGCCGTGTGAACAAAGCCT
  TGGATAAGACCCATCGTCAGGGGGCCTCCTTCCTGGGCATTCTGGATATTGCTGGGTTTGAAATCTTTGA
  GGTAAACTCCTTCGAGCAGCTGTGCATCAACTACACCAATGAGAAGTTGCAGCAGCTGTTCAATCACACC
  ATGTTCATCCTGGAGCAGGAAGAGTACCAACGAGAGGGCATCGAGTGGAACTTCATCGACTTTGGCCTGG
  ACCTACAGCCTTGTATTGAACTGATTGAGCGGCCGAACAACCCTCCTGGTGTGCTGGCCCTGCTGGATGA
  AGAATGCTGGTTCCCCAAAGCTACGGACAAGTCTTTTGTGGAGAAGCTGTGCTCAGAGCAGGGCAATCAC
  CCCAAATTTCAGAAGCCCAAGCAGCTAAAGGACAAAACAGAGTTCTCCATCATCCACTATGCTGGGAAGG
  TGGACTATAATGCAAGTGCGTGGCTGACCAAGAACATGGACCCGCTAAATGATAATGTGACCTCACTCCT
  CAATGCCTCCTCGGACAAGTTTGTGGCCGACCTGTGGAAGGATGTGGACCGCATTGTGGGGTTGGACCAG
  ATGGCCAAGATGACTGAGAGCTCACTGCCCAGTGCCTCCAAGACCAAAAAGGGCATGTTCCGCACCGTGG
  GCCAGCTCTACAAAGAGCAGCTGGGGAAGCTGATGACCACACTGCGCAACACCACGCCTAACTTTGTGCG
  CTGCATCATCCCCAACCATGAAAAGAGGTCTGGCAAGCTGGATGCATTTCTAGTTCTGGAGCAGCTGCGG
  TGCAATGGTGTGTTGGAAGGCATCCGCATCTGCCGTCAGGGCTTCCCCAACAGGATTGTCTTCCAAGAGT
  TCCGGCAACGCTACGAGATCCTGGCAGCGAACGCCATCCCCAAAGGCTTCATGGACGGAAAGCAAGCCTG
  CATTCTCATGATCAAAGCCCTTGAACTTGACCCCAATCTGTACAGGATTGGGCAGAGCAAAATCTTCTTT
  CGTACGGGAGTCCTGGCCCACCTAGAGGAAGAACGAGACCTGAAGATTACTGATGTCATCATGGCTTTCC
  AGGCAATGTGTCGTGGCTACCTGGCCAGAAAAGCCTTCACCAAGAGGCAGCAACAGTTAACAGCCATGAA
  GGTGATCCAGAGGAACTGCGCTGCCTACCTTAAGCTCCGCAACTGGCAATGGTGGAGGCTCTTCACTAAA
  GTAAAGCCGCTGCTCCAGGTGACGCGGCAGGAGGAGGAGATGCAGGCCAAGGAGGAGGAGATGCAGAAGA
  TCAAGGAGCGGCAGCAGAAGGCAGAGTCTGAGCTGAAGGAGCTGGAGCAAAGACACACTCAGCTGGCTGA
  GGAGAAGACCCTGCTGCAGGAGCAGTTGCAGGCAGAGACAGAGCTGTATGCTGAGGCTGAAGAGATGCGG
  GTCCGCTTGGCAGCGAAGAAGCAGGAGCTAGAGGAGATCCTCCATGAGATGGAGGCCCGCTTGGAGGAGG
  AGGAGGACCGGAGCCAACAACTGCAGGCTGAGAGGAAGAAGATGGCTCAGCAGATGCTGGACCTGGAGGA
  GCAACTGGAGGAGGAAGAAGCTGCCAGACAGAAACTACAGCTAGAGAAGGTCACAGCTGAGGCCAAGATC
  AAGAAACTGGAGGATGACATCTTGGTTATGGATGACCAGAACAATAAGCTTTCCAAAGAAAGAAAACTCC
  TTGAAGAGAGGGTCAGCGACTTGACAACCAACCTAGCAGAAGAGGAAGAAAAGGCTAAAAACCTCACAAA
  ACTAAAGAGCAAGCATGAGTCCATGATCTCAGAGCTAGAGGTGAGGCTGAAGAAAGAGGAGAAGAGCCGG
  CAGGAGCTGGAGAAACTCAAGAGGAAACTGGAGGGTGATGCCAGTGACTTCCACGAGCAGATCGCTGACC
  TGCAGGCCCAGATTGCAGAGCTCAAGATGCAGCTGGCAAAGAAAGAGGAAGAGCTACAGGCAGCTCTAGC
  CAGGCTCGATGAAGAGATCACCCAGAAAAACAATGCCCTAAAGAAGATTCGAGAGCTGGAGGGCCATGTC
  TCAGACCTACAGGAGGACCTGGACTCAGAGCGAGCTGCTAGGAACAAGGCTGAGAAACAGAAGCGAGACC
  TGGGGGAGGAACTGGAGGCGCTCAAGACAGAGCTGGAAGATACACTGGACAGCACAGCTACCCAGCAGGA
  GCTCAGAGCCAAGAGGGAACAGGAAGTGACAATGCTGAAGAAGGCCCTGGATGAAGAGACGCGATCCCAT
  GAGGCCCAGGTCCAGGAGATGAGGCAGAAGCACACACAGGCAGTGGAGGAACTCACAGAGCAGCTGGAAC
  AGTTCAAAAGGGCCAAGGCAAACCTGGATAAAAGCAAGCAGACGCTGGAGAAGGAGAACGCAGACCTGGC
  TGGGGAGCTGCGTGTCCTGGGCCAGGCAAAGCAGGAGGTAGAGCACAAGAAGAAGAAGCTGGAGGGGCAG
  CTGCAGGAGCTGCAGTCCAAGTGCAGTGATGGGGAGCGTGCCCGGACTGAGCTCAGTGACAAGGTCCACA
  AACTACAGAATGAAGTGGAGAGTGTCACAGGCATGCTCAATGAGGCAGAAGGCAAGGCCATTAAACTGGC
  CAAGGAAGTGGCTTCCCTTGGATCTCAACTTCAGGACACCCAAGAGCTGCTTCAAGAAGAAACCCGGCAG
  AAGCTCAATGTGTCCACCAAGCTGCGCCAGTTGGAAGATGAGAGGAACAGCCTGCAGGACCAGCTGGATG
  AGGAGATGGAAGCTAAGCAAAACCTTGAGCGCCATGTCTCCACCTTGAACATTCAGCTCTCAGACTCTAA
  GAAGAAGCTGCAGGACCTTGCAAGCACTATTGAGGTCATGGAGGAGGGGAAGAAGAGGTTGCAGAAAGAG
  ATGGAGGGTCTCGGCCAGCAGTATGAGGAGAAGGCCGCTGCCTATGACAAACTGGAGAAAACTAAGAACA
  GGCTTCAGCAGGAGCTAGATGACTTGGTCGTGGACTTGGACAACCAGCGGCAACTGGTATCCAATCTGGA
  AAAGAAGCAGAAGAAATTCGATCAGTTGTTAGCTGAGGAAAAGAACATCTCCTCCAAGTATGCGGATGAG
  AGAGACCGAGCTGAAGCAGAGGCCAGGGAAAAGGAAACGAAGGCTTTGTCTCTGGCCCGGGCCCTGGAGG
  AAGCCCTGGAAGCCAAAGAGGAACTGGAAAGGACCAACAAGATGCTCAAGGCCGAGATGGAAGACCTGGT
  CAGCTCCAAGGATGACGTGGGCAAGAATGTGCACGAGCTGGAGAAGTCCAAACGAGCCCTGGAGACCCAG
  ATGGAAGAGATGAGAACTCAGCTAGAGGAGCTGGAGGACGAGCTGCAGGCCACTGAAGACGCCAAGCTGC
  GGCTGGAGGTCAACATGCAGGCCCTCAAGGGCCAGTTTGAACGAGATCTCCAGGCTCGGGATGAGCAGAA
  TGAGGAGAAGAGGAGGCAGCTACAGCGGCAGCTGCATGAGTATGAGACAGAACTGGAAGATGAACGGAAG
  CAAAGGGCTCTGGCGGCAGCAGCCAAGAAGAAGCTGGAAGGGGATCTGAAAGACCTGGAACTCCAGGCTG
  ACTCAGCCGTCAAAGGGAGGGAGGAAGCCATCAAGCAGCTTCGAAAACTGCAGGCTCAGATGAAGGACTT
  CCAAAGAGAGCTGGATGATGCCCGTGCCTCCAGAGATGAGATCTTTGCCACCTCAAAAGAGAATGAGAAG
  AAAGCCAAGAGTCTGGAGGCAGAGCTCATGCAGCTCCAGGAGGACCTGGCAGCAGCTGAGAGAGCTCGCA
  AGCAAGCTGACCTGGAGAAGGAGGAGCTGGCTGAGGAGCTGGCTAGCAGCTTATCAGGAAGGAATACACT
  GCAGGATGAGAAGCGCCGCCTGGAGGCAAGGATCGCCCAGCTGGAGGAGGAGCTGGAGGAAGAGCAGGGC
  AACATGGAGGCCATGAGTGACAGAGTCCGCAAGGCCACATTGCAGGCTGAGCAACTGAGCAATGAGCTGG
  TCACTGAGCGCAGTGCAGCTCAGAAGAATGAGAGTGCACGGCAACAGCTGGAGCGCCAGAACAAGGAACT
  CCGAAGCAAGTTGCAGGAGGTAGAAGGTGCTGTCAAAGCCAAGCTTAAGTCCACTGTTGCGGCACTAGAG
  GCCAAGATTGTGCAGCTGGAGGAGCAGATTGAACAAGAGGCCAGAGAAAAACAGGCAGCCACCAAGTTGC
  TGAAGCAAAAGGACAAGAAGCTAAAGGAGGTCCTACTGCAGGTGGAGGATGAGCGCAAGATGGTGGAGCA
  GTACAAGGAGCAGGCAGAGAAAGGAAACACCAAGGTCAAGCAGCTCAAGAGGCAGCTGGAAGAGGCCGAG
  GAGGAATCCCAGCGCATCAATGCCAACCGCAGGAAGCTGCAGCGGGAGCTGGATGAGGCCACTGAGAGCA
  ATGAGGCCATGGGCCGTGAGGTGAATGCCCTCAAGAGCAAACTCAGGAGAGGAAACGAGGCTTCATTTGT
  TCCTTCCAGAAGGGCTGGAGGCCGTAGAGTTATTGAAAACACAGATGGTTCTGAAGAAGAAATGGATGCT
  CGGGACTCAGACTTCAATGGAACCAAAGCCAGTGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR217716 representing NM_001170600
  Red=Cloning site Green=Tags(s)
  
  MAQKGQLSDDEKFLFVDKNFINSPMAQADWVAKKLVWVPSEKQGFEAASIKEEKGDEVVVELVENGKKVT
  VGKDDIQKMNPPKFSKVEDMAELTCLNEASVLHNLRERYFSGLIYTYSGLFCVVVNPYKHLPIYSEKIVD
  MYKGKKRHEMPPHIYAIADTAYRSMLQDREDQSILCTGESGAGKTENTKKVIQYLAVVASSHKGKKDSSI
  TGELEKQLLQANPILEAFGNAKTVKNDNSSRFGKFIRINFDVTGYIVGANIETYLLEKSRAIRQARDERT
  FHIFYYLIAGAKEKMRNDLLLESFNSYTFLSNGFVPIPAAQDDEMFQETLEAMSIMGFSEEEQLAILKVV
  SSVLQLGNIVFKKERNTDQASMPDNTAAQKVCHLVGINVTDFTRAILTPRIKVGRDVVQKAQTKEQADFA
  IEALAKATYERLFRWILSRVNKALDKTHRQGASFLGILDIAGFEIFEVNSFEQLCINYTNEKLQQLFNHT
  MFILEQEEYQREGIEWNFIDFGLDLQPCIELIERPNNPPGVLALLDEECWFPKATDKSFVEKLCSEQGNH
  PKFQKPKQLKDKTEFSIIHYAGKVDYNASAWLTKNMDPLNDNVTSLLNASSDKFVADLWKDVDRIVGLDQ
  MAKMTESSLPSASKTKKGMFRTVGQLYKEQLGKLMTTLRNTTPNFVRCIIPNHEKRSGKLDAFLVLEQLR
  CNGVLEGIRICRQGFPNRIVFQEFRQRYEILAANAIPKGFMDGKQACILMIKALELDPNLYRIGQSKIFF
  RTGVLAHLEEERDLKITDVIMAFQAMCRGYLARKAFTKRQQQLTAMKVIQRNCAAYLKLRNWQWWRLFTK
  VKPLLQVTRQEEEMQAKEEEMQKIKERQQKAESELKELEQRHTQLAEEKTLLQEQLQAETELYAEAEEMR
  VRLAAKKQELEEILHEMEARLEEEEDRSQQLQAERKKMAQQMLDLEEQLEEEEAARQKLQLEKVTAEAKI
  KKLEDDILVMDDQNNKLSKERKLLEERVSDLTTNLAEEEEKAKNLTKLKSKHESMISELEVRLKKEEKSR
  QELEKLKRKLEGDASDFHEQIADLQAQIAELKMQLAKKEEELQAALARLDEEITQKNNALKKIRELEGHV
  SDLQEDLDSERAARNKAEKQKRDLGEELEALKTELEDTLDSTATQQELRAKREQEVTMLKKALDEETRSH
  EAQVQEMRQKHTQAVEELTEQLEQFKRAKANLDKSKQTLEKENADLAGELRVLGQAKQEVEHKKKKLEGQ
  LQELQSKCSDGERARTELSDKVHKLQNEVESVTGMLNEAEGKAIKLAKEVASLGSQLQDTQELLQEETRQ
  KLNVSTKLRQLEDERNSLQDQLDEEMEAKQNLERHVSTLNIQLSDSKKKLQDLASTIEVMEEGKKRLQKE
  MEGLGQQYEEKAAAYDKLEKTKNRLQQELDDLVVDLDNQRQLVSNLEKKQKKFDQLLAEEKNISSKYADE
  RDRAEAEAREKETKALSLARALEEALEAKEELERTNKMLKAEMEDLVSSKDDVGKNVHELEKSKRALETQ
  MEEMRTQLEELEDELQATEDAKLRLEVNMQALKGQFERDLQARDEQNEEKRRQLQRQLHEYETELEDERK
  QRALAAAAKKKLEGDLKDLELQADSAVKGREEAIKQLRKLQAQMKDFQRELDDARASRDEIFATSKENEK
  KAKSLEAELMQLQEDLAAAERARKQADLEKEELAEELASSLSGRNTLQDEKRRLEARIAQLEEELEEEQG
  NMEAMSDRVRKATLQAEQLSNELVTERSAAQKNESARQQLERQNKELRSKLQEVEGAVKAKLKSTVAALE
  AKIVQLEEQIEQEAREKQAATKLLKQKDKKLKEVLLQVEDERKMVEQYKEQAEKGNTKVKQLKRQLEEAE
  EESQRINANRRKLQRELDEATESNEAMGREVNALKSKLRRGNEASFVPSRRAGGRRVIENTDGSEEEMDA
  RDSDFNGTKASE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001170600
• ORF Size: 5916 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001170600.1, NP_001164071.1
• RefSeq Size: 6555 bp
• RefSeq ORF: 5919 bp
• Locus ID: 24582
• Cytogenetics: 10q11
• MW: 227.3 kDa
• Gene Summary: transcription decreases following transformation with mutant p53 [RGD, Feb 2006]