Myh11 (NM_001170600) Rat Untagged Clone

CAT#: RN217716

Myh11 (untagged) - Rat myosin, heavy chain 11, smooth muscle (Myh11)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: Myh11
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN217716 representing NM_001170600
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCACAGAAAGGGCAGCTCAGTGATGATGAGAAGTTCCTCTTTGTGGATAAAAACTTCATAAACAGCC
  CAATGGCCCAGGCCGACTGGGTAGCCAAGAAGCTAGTATGGGTCCCTTCAGAGAAGCAGGGCTTCGAAGC
  AGCCAGCATCAAGGAGGAGAAGGGCGATGAGGTGGTTGTAGAGCTGGTGGAAAACGGAAAGAAGGTCACA
  GTGGGCAAAGATGACATCCAAAAAATGAACCCGCCCAAGTTCTCCAAGGTGGAAGACATGGCCGAGCTGA
  CATGCCTCAATGAGGCTTCTGTGCTGCACAACCTGAGGGAGCGGTACTTCTCAGGCCTCATCTACACCTA
  CTCGGGCCTCTTCTGTGTGGTGGTCAACCCCTACAAGCACCTACCCATCTACTCAGAAAAGATTGTGGAC
  ATGTACAAGGGCAAGAAGAGGCATGAGATGCCGCCTCACATCTATGCCATTGCTGATACGGCCTACAGAA
  GCATGCTACAAGATCGTGAAGACCAGTCCATTCTGTGCACAGGTGAGTCTGGAGCTGGAAAGACAGAGAA
  CACAAAGAAAGTCATACAGTATTTGGCTGTGGTGGCATCCTCCCACAAAGGCAAGAAAGACAGCAGCATC
  ACGGGGGAGCTGGAAAAGCAGCTTCTACAGGCAAACCCAATCCTGGAGGCTTTCGGCAATGCAAAAACTG
  TCAAGAATGACAACTCCTCCCGCTTTGGCAAGTTCATTCGCATCAACTTTGATGTCACTGGTTACATTGT
  GGGTGCCAATATCGAAACATATCTTCTGGAAAAGTCTCGGGCTATTCGACAGGCTAGGGACGAGAGAACG
  TTCCACATCTTCTACTATCTGATCGCTGGAGCCAAGGAGAAGATGAGAAATGACCTACTTTTGGAGAGCT
  TCAACAGCTACACATTTTTATCCAATGGCTTTGTGCCCATCCCAGCTGCACAAGATGATGAGATGTTCCA
  GGAGACACTAGAGGCCATGTCCATCATGGGCTTCAGTGAAGAGGAACAGCTAGCCATCTTGAAGGTAGTA
  TCATCCGTCCTTCAGCTTGGAAACATTGTCTTCAAGAAGGAGCGAAACACAGACCAGGCATCCATGCCTG
  ATAACACAGCTGCTCAGAAAGTTTGCCACCTTGTGGGGATTAATGTGACAGATTTCACCAGAGCCATCCT
  GACCCCACGTATAAAAGTTGGACGGGATGTGGTGCAGAAAGCTCAGACCAAAGAACAGGCTGACTTTGCC
  ATTGAGGCCTTAGCCAAGGCCACCTATGAGCGCCTTTTCCGATGGATTCTCAGCCGTGTGAACAAAGCCT
  TGGATAAGACCCATCGTCAGGGGGCCTCCTTCCTGGGCATTCTGGATATTGCTGGGTTTGAAATCTTTGA
  GGTAAACTCCTTCGAGCAGCTGTGCATCAACTACACCAATGAGAAGTTGCAGCAGCTGTTCAATCACACC
  ATGTTCATCCTGGAGCAGGAAGAGTACCAACGAGAGGGCATCGAGTGGAACTTCATCGACTTTGGCCTGG
  ACCTACAGCCTTGTATTGAACTGATTGAGCGGCCGAACAACCCTCCTGGTGTGCTGGCCCTGCTGGATGA
  AGAATGCTGGTTCCCCAAAGCTACGGACAAGTCTTTTGTGGAGAAGCTGTGCTCAGAGCAGGGCAATCAC
  CCCAAATTTCAGAAGCCCAAGCAGCTAAAGGACAAAACAGAGTTCTCCATCATCCACTATGCTGGGAAGG
  TGGACTATAATGCAAGTGCGTGGCTGACCAAGAACATGGACCCGCTAAATGATAATGTGACCTCACTCCT
  CAATGCCTCCTCGGACAAGTTTGTGGCCGACCTGTGGAAGGATGTGGACCGCATTGTGGGGTTGGACCAG
  ATGGCCAAGATGACTGAGAGCTCACTGCCCAGTGCCTCCAAGACCAAAAAGGGCATGTTCCGCACCGTGG
  GCCAGCTCTACAAAGAGCAGCTGGGGAAGCTGATGACCACACTGCGCAACACCACGCCTAACTTTGTGCG
  CTGCATCATCCCCAACCATGAAAAGAGGTCTGGCAAGCTGGATGCATTTCTAGTTCTGGAGCAGCTGCGG
  TGCAATGGTGTGTTGGAAGGCATCCGCATCTGCCGTCAGGGCTTCCCCAACAGGATTGTCTTCCAAGAGT
  TCCGGCAACGCTACGAGATCCTGGCAGCGAACGCCATCCCCAAAGGCTTCATGGACGGAAAGCAAGCCTG
  CATTCTCATGATCAAAGCCCTTGAACTTGACCCCAATCTGTACAGGATTGGGCAGAGCAAAATCTTCTTT
  CGTACGGGAGTCCTGGCCCACCTAGAGGAAGAACGAGACCTGAAGATTACTGATGTCATCATGGCTTTCC
  AGGCAATGTGTCGTGGCTACCTGGCCAGAAAAGCCTTCACCAAGAGGCAGCAACAGTTAACAGCCATGAA
  GGTGATCCAGAGGAACTGCGCTGCCTACCTTAAGCTCCGCAACTGGCAATGGTGGAGGCTCTTCACTAAA
  GTAAAGCCGCTGCTCCAGGTGACGCGGCAGGAGGAGGAGATGCAGGCCAAGGAGGAGGAGATGCAGAAGA
  TCAAGGAGCGGCAGCAGAAGGCAGAGTCTGAGCTGAAGGAGCTGGAGCAAAGACACACTCAGCTGGCTGA
  GGAGAAGACCCTGCTGCAGGAGCAGTTGCAGGCAGAGACAGAGCTGTATGCTGAGGCTGAAGAGATGCGG
  GTCCGCTTGGCAGCGAAGAAGCAGGAGCTAGAGGAGATCCTCCATGAGATGGAGGCCCGCTTGGAGGAGG
  AGGAGGACCGGAGCCAACAACTGCAGGCTGAGAGGAAGAAGATGGCTCAGCAGATGCTGGACCTGGAGGA
  GCAACTGGAGGAGGAAGAAGCTGCCAGACAGAAACTACAGCTAGAGAAGGTCACAGCTGAGGCCAAGATC
  AAGAAACTGGAGGATGACATCTTGGTTATGGATGACCAGAACAATAAGCTTTCCAAAGAAAGAAAACTCC
  TTGAAGAGAGGGTCAGCGACTTGACAACCAACCTAGCAGAAGAGGAAGAAAAGGCTAAAAACCTCACAAA
  ACTAAAGAGCAAGCATGAGTCCATGATCTCAGAGCTAGAGGTGAGGCTGAAGAAAGAGGAGAAGAGCCGG
  CAGGAGCTGGAGAAACTCAAGAGGAAACTGGAGGGTGATGCCAGTGACTTCCACGAGCAGATCGCTGACC
  TGCAGGCCCAGATTGCAGAGCTCAAGATGCAGCTGGCAAAGAAAGAGGAAGAGCTACAGGCAGCTCTAGC
  CAGGCTCGATGAAGAGATCACCCAGAAAAACAATGCCCTAAAGAAGATTCGAGAGCTGGAGGGCCATGTC
  TCAGACCTACAGGAGGACCTGGACTCAGAGCGAGCTGCTAGGAACAAGGCTGAGAAACAGAAGCGAGACC
  TGGGGGAGGAACTGGAGGCGCTCAAGACAGAGCTGGAAGATACACTGGACAGCACAGCTACCCAGCAGGA
  GCTCAGAGCCAAGAGGGAACAGGAAGTGACAATGCTGAAGAAGGCCCTGGATGAAGAGACGCGATCCCAT
  GAGGCCCAGGTCCAGGAGATGAGGCAGAAGCACACACAGGCAGTGGAGGAACTCACAGAGCAGCTGGAAC
  AGTTCAAAAGGGCCAAGGCAAACCTGGATAAAAGCAAGCAGACGCTGGAGAAGGAGAACGCAGACCTGGC
  TGGGGAGCTGCGTGTCCTGGGCCAGGCAAAGCAGGAGGTAGAGCACAAGAAGAAGAAGCTGGAGGGGCAG
  CTGCAGGAGCTGCAGTCCAAGTGCAGTGATGGGGAGCGTGCCCGGACTGAGCTCAGTGACAAGGTCCACA
  AACTACAGAATGAAGTGGAGAGTGTCACAGGCATGCTCAATGAGGCAGAAGGCAAGGCCATTAAACTGGC
  CAAGGAAGTGGCTTCCCTTGGATCTCAACTTCAGGACACCCAAGAGCTGCTTCAAGAAGAAACCCGGCAG
  AAGCTCAATGTGTCCACCAAGCTGCGCCAGTTGGAAGATGAGAGGAACAGCCTGCAGGACCAGCTGGATG
  AGGAGATGGAAGCTAAGCAAAACCTTGAGCGCCATGTCTCCACCTTGAACATTCAGCTCTCAGACTCTAA
  GAAGAAGCTGCAGGACCTTGCAAGCACTATTGAGGTCATGGAGGAGGGGAAGAAGAGGTTGCAGAAAGAG
  ATGGAGGGTCTCGGCCAGCAGTATGAGGAGAAGGCCGCTGCCTATGACAAACTGGAGAAAACTAAGAACA
  GGCTTCAGCAGGAGCTAGATGACTTGGTCGTGGACTTGGACAACCAGCGGCAACTGGTATCCAATCTGGA
  AAAGAAGCAGAAGAAATTCGATCAGTTGTTAGCTGAGGAAAAGAACATCTCCTCCAAGTATGCGGATGAG
  AGAGACCGAGCTGAAGCAGAGGCCAGGGAAAAGGAAACGAAGGCTTTGTCTCTGGCCCGGGCCCTGGAGG
  AAGCCCTGGAAGCCAAAGAGGAACTGGAAAGGACCAACAAGATGCTCAAGGCCGAGATGGAAGACCTGGT
  CAGCTCCAAGGATGACGTGGGCAAGAATGTGCACGAGCTGGAGAAGTCCAAACGAGCCCTGGAGACCCAG
  ATGGAAGAGATGAGAACTCAGCTAGAGGAGCTGGAGGACGAGCTGCAGGCCACTGAAGACGCCAAGCTGC
  GGCTGGAGGTCAACATGCAGGCCCTCAAGGGCCAGTTTGAACGAGATCTCCAGGCTCGGGATGAGCAGAA
  TGAGGAGAAGAGGAGGCAGCTACAGCGGCAGCTGCATGAGTATGAGACAGAACTGGAAGATGAACGGAAG
  CAAAGGGCTCTGGCGGCAGCAGCCAAGAAGAAGCTGGAAGGGGATCTGAAAGACCTGGAACTCCAGGCTG
  ACTCAGCCGTCAAAGGGAGGGAGGAAGCCATCAAGCAGCTTCGAAAACTGCAGGCTCAGATGAAGGACTT
  CCAAAGAGAGCTGGATGATGCCCGTGCCTCCAGAGATGAGATCTTTGCCACCTCAAAAGAGAATGAGAAG
  AAAGCCAAGAGTCTGGAGGCAGAGCTCATGCAGCTCCAGGAGGACCTGGCAGCAGCTGAGAGAGCTCGCA
  AGCAAGCTGACCTGGAGAAGGAGGAGCTGGCTGAGGAGCTGGCTAGCAGCTTATCAGGAAGGAATACACT
  GCAGGATGAGAAGCGCCGCCTGGAGGCAAGGATCGCCCAGCTGGAGGAGGAGCTGGAGGAAGAGCAGGGC
  AACATGGAGGCCATGAGTGACAGAGTCCGCAAGGCCACATTGCAGGCTGAGCAACTGAGCAATGAGCTGG
  TCACTGAGCGCAGTGCAGCTCAGAAGAATGAGAGTGCACGGCAACAGCTGGAGCGCCAGAACAAGGAACT
  CCGAAGCAAGTTGCAGGAGGTAGAAGGTGCTGTCAAAGCCAAGCTTAAGTCCACTGTTGCGGCACTAGAG
  GCCAAGATTGTGCAGCTGGAGGAGCAGATTGAACAAGAGGCCAGAGAAAAACAGGCAGCCACCAAGTTGC
  TGAAGCAAAAGGACAAGAAGCTAAAGGAGGTCCTACTGCAGGTGGAGGATGAGCGCAAGATGGTGGAGCA
  GTACAAGGAGCAGGCAGAGAAAGGAAACACCAAGGTCAAGCAGCTCAAGAGGCAGCTGGAAGAGGCCGAG
  GAGGAATCCCAGCGCATCAATGCCAACCGCAGGAAGCTGCAGCGGGAGCTGGATGAGGCCACTGAGAGCA
  ATGAGGCCATGGGCCGTGAGGTGAATGCCCTCAAGAGCAAACTCAGGAGAGGAAACGAGGCTTCATTTGT
  TCCTTCCAGAAGGGCTGGAGGCCGTAGAGTTATTGAAAACACAGATGGTTCTGAAGAAGAAATGGATGCT
  CGGGACTCAGACTTCAATGGAACCAAAGCCAGTGAATAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_001170600
• Insert Size: 5919 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001170600.1, NP_001164071.1
• RefSeq Size: 6555 bp
• RefSeq ORF: 5919 bp
• Locus ID: 24582
• Cytogenetics: 10q11
• Gene Summary: transcription decreases following transformation with mutant p53 [RGD, Feb 2006]