Adgrl2 (NM_001302208) Rat Tagged ORF Clone

CAT#: RR217635

Lphn2 (myc-DDK-tagged) - Rat latrophilin 2 (Lphn2), transcript variant 3

Product Images

Circular map for RR217635

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Adgrl2
• Synonyms: Cirl-2; Cirl2; Cl2ac; Lphn2
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR217635 representing NM_001302208
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTGTCTTCTGGTTGCAGAATGCGAAGTCTCTGGTTTATCATGATAATCAGTTTCTCACCGAATACCG
  AAGGTTTCAGCAGAGCAGCCTTGCCATTCGGGTTAGTTAGACGAGAGCTGTCCTGTGAAGGTTATTCTAT
  AGACCTGCGATGTCCGGGCAGTGACGTCATCATGATCGAGAGCGCAAACTACGGTCGGACGGACGACAAG
  ATCTGCGACGCAGACCCCTTTCAGATGGAGAACACAGACTGCTACCTCCCTGATGCCTTCAAAATCATGA
  CTCAAAGGTGCAACAACCGAACACAGTGTGTAGTAGTTACCGGGTCAGATGTATTTCCTGATCCATGTCC
  TGGAACTTACAAATACCTTGAAGTTCAATATGAATGTGTCCCTTACATGGAGCAAAAAGTTTTTGTGTGT
  CCTGGAACCTTGAAAGCAATTGTGGACTCTCCAAGTATCTATGAAGCTGAGCAAAAGGCAGGTGCTTGGT
  GCAAGGACCCCCTTCAGGCTGCAGATAAAATTTATTTTATGCCCTGGACTCCCTACCGCACCGATACCTT
  AATAGAATATGCTTCTTTAGAAGATTTTCAAAACAGCCGCCAGACAACAACATACAAACTTCCAAACCGA
  GTGGACGGTACTGGATTTGTGGTGTATGACGGGGCAGTCTTCTTCAACAAAGAAAGAACGAGAAACATTG
  TTAAATTTGACTTGAGGACTAGAATCAAGAGTGGGGAGGCCATAATCAACTACGCCAACTACCATGACAC
  TTCACCCTACAGATGGGGGGGGAAGACTGACATTGACCTGGCAGTGGATGAAAATGGCTTGTGGGTCATC
  TACGCCACCGAGCAGAACAACGGAATGATCGTGATTAGCCAGCTCAATCCGTACACTCTCCGATTCGAAG
  CAACCTGGGAGACGACGTATGACAAGCGTGCGGCGTCCAATGCTTTCATGATATGCGGGGTCCTCTACGT
  GGTCAGGTCAGTGTACCAAGACAATGAAAGCGAAGCTGGCAAGAACGTCATCGACTACATTTACAACACA
  AGGTTGAGCCGGGGAGAGCACGTGGACGTTCCCTTCCCCAACCAGTACCAGTACATCGCTGCAGTGGATT
  ACAACCCAAGAGACAACCAACTCTACGTATGGAACAATAACTTTATCTTACGGTATTCTCTGGAGTTTGG
  TCCACCCGACCCTGCCCAAGTGCCTACCACAGCTGTGACAATAACTTCTTCAGCTGAGCTGTTCAAAACC
  ACAGTGTCAACCACAAGCAGTACTTCACAGAGAGGCCCCGTGAGCAGCACAGTCGCTGGTCCTCAGGAAG
  GAAGCCGAGGGACAAAGCCACCTCCAGCAGTCTCTACAACCAAAATTCCTCCTGTAACAAATATTTTTCC
  CCTGCCAGAGAGATTCTGCGAAGCGTTAGAAATGAAGGGGATAAAGTGGCCTCAGACACAAAGGGGGATG
  ATGGTTGAGCGACCGTGTCCCAAGGGAACAAGAGGAACGGCCTCGTATCTCTGCATGGCTTCCACAGGAA
  CCTGGAACCCGAAGGGCCCGGATCTTAGCAACTGCACCTCTCACTGGGTGAATCAGCTGGCCCAGAAGAT
  CAGAAGTGGAGAGAATGCTGCAAGTCTGGCCAACGAACTGGCTAAGCACACCAAGGGGACGGTGTTCGCT
  GGGGATGTGAGCTCCTCTGTGAGGCTGATGGAACAGTTGGTGGACATCCTGGATGCCCAGCTGCAGGAGC
  TGAAACCGAGCGAGAAGGACTCGGCCGGGAGGAGTTATAACAAGCTCCAAAAACGAGAGAAGACATGCAG
  GGCTTACCTTAAGGCCATTGTGGACACAGTAGATAACCTTCTGAGAGCCGAGACTTTGGACTGCTGGAAA
  CACATGAATTCCTCAGAGCAGGCGCACACAGCCACCATGCTGTTGGACACCTTGGAAGAAGGAGCATTTG
  TCCTGGCAGACAACCTTTTGGAACCAACCCGGGTCTCAATGCCAACGGATAATATTGTTCTAGAAGTCGC
  TGTCCTCAGCACGGAAGGACAGGTCCAAGACTTCACCTTCCATCTCGGCTTCAAGGGGGCCTTCAGCTCC
  ATCCAGCTCTCAGCCAACACCGTCAAGCAAAACAGCAGAAACGGGCTGGCAAAGGTGGTATTCATCATTT
  ACCGGAGTCTGGGACCATTCCTGAGCACCGAAAATGCGACCGTCAAACTGGGCGCAGACCTCCTGGGTCG
  GAACAGCACCATCGCAGTGAACTCGCACGTCCTTTCAGTCTCCATCAATAAGGAGTCCAGCCGTGTGTAC
  TTGACAGACCCGGTGCTTTTTTCAATGCCACACATTGATTCTGACAATTATTTCAACGCAAACTGCTCCT
  TCTGGAACTACTCAGAGAGAACCATGATGGGATATTGGTCTACCCAGGGCTGCAAGCTGGTTGACACTAA
  TAAAACTCGCACGACGTGTGCATGCAGCCACCTAACCAATTTTGCTATTCTCATGGCCCACAGGGAAATT
  GTGTACAAAGATGGCGTCCACAAATTGCTGCTGACAGTCATCACCTGGGTGGGCATCGTTGTCTCCCTCG
  TCTGCCTGGCTATCTGCATCTTCACCTTCTGCTTCTTCCGAGGCCTGCAAAGCGACCGCAACACGATCCA
  CAAGAACCTGTGTATCAACCTCTTCATCGCTGAGTTTATTTTCCTAATAGGCATTGATAAAACACAGTAC
  ACGATTGCGTGCCCCGTGTTTGCAGGACTCCTGCACTTTTTCTTCCTGGCTGCTTTTTCCTGGATGTGCC
  TAGAAGGTGTGCAGCTCTACCTCATGTTGGTTGAAGTTTTCGAGAGTGAATACTCAAGGAAGAAGTATTA
  CTATGTCGCCGGGTACCTCTTCCCTGCCACAGTGGTCGGTGTTTCAGCTGCTATCGACTACAAGAGTTAC
  GGGACACTAGAGGCTTGCTGGCTTCACGTTGATAACTATTTCATATGGAGTTTCATTGGGCCTGTTACTT
  TCATCATTCTGCTAAATATTATTTTCCTGGTGATCACGCTGTGCAAAATGGTGAAACATTCAAACACTTT
  GAAACCAGATTCTAGCAGGTTGGAAAACATTAATAATTACCGTGTTTGTGATGGATACTATAATACGGAC
  TTACCTGGGTCTTGGGTGCTCGGTGCGTTCGCCCTGCTGTGTCTCCTGGGCCTAACCTGGTCCTTTGGGT
  TGCTTTTTGTTAACGAGGAGACCGTTGTCATGGCTTATCTCTTCACCGCCTTTAATGCTTTCCAGGGACT
  GTTTATTTTCATCTTCCACTGTGCTCTTCAAAAGAAAGTACGGAAAGAGTATGCCAAGTGCTTCAGACAC
  TGGTACTGCTGTGGTGGCCTCCCGACCGAGAGCCCGCACAGCTCTGTAAAGGCGTCCACCTCCCGCACCA
  GTGCTCGCTACTCCTCTGGTACACAGAGCCGTATAAGAAGGATGTGGAATGACACCGTGAGGAAGCAGTC
  TGAATCGTCTTTTATCTCAGGAATGACTGGCAATTACCTACTAACAAACCCTCTTCTTCGACCCCACGGC
  ACTAACAACCCCTATAACACATTGCTCGCTGAAACAGTTGTATGTAATGCCCCTTCAGCGCCCGTGTTTA
  ACTCACCAGGACATTCACTGAACAATACCCGGGACACCAGCGCCATGGATACTCTACCGCTAAATGGTAA
  CTTCAACAACAGCTACTCCCTGCGCAAGGCCGACTACCACGACGGCGTGCAGGTTGTGGACTGTGGACTA
  AGTCTGAACGACACCGCGTTTGAGAAAATGATCATTTCAGAGTTAGTGCACAACAACCTCCGGGGTAGCA
  ACAAAACCCACAACTTGGAGCTCAAGCTCCCGGTTAAACCCGTGATTGGCGGCAGCAGCAGCGAAGATGA
  CGCGATCGTGGCCGACGCCTCATCTTTGATGCACGGTGATAACCCAGGGCTGGAATTCCGCCACAAAGAG
  CTGGAGGCACCGCTCATCCCTCAGCGGACTCACTCGCTTCTGTACCAACCCCAGAAAAAAGTGAAACCCG
  AGGCAACCGACAGCTACGTCTCCCAGCTGACGGCCGAGGCCGACGAGCACCTCCAGTCCCCCAACAGAGA
  CTCTCTGTACACGAGCATGCCCAACCTAAGAGACTCTCCCTACCCGGAGAGCAGCCCGGACATGGCAGAG
  GACCTGTCTCCCTCCAGGAGGAGCGAGAACGAGGACATTTACTACAAAAGTATGCCCAATCTTGGGGCTG
  GCCGCCAGCTCCAGATGTGCTACCAGATCAGCAGAGGCAATAGCGATGGCTACATCATCCCCATTAACAA
  AGAAGGGTGCATCCCAGAGGGGGACGTCAGGGAAGGACAGATGCAGCTGGTAACAAGTCTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR217635 representing NM_001302208
  Red=Cloning site Green=Tags(s)
  
  MVSSGCRMRSLWFIMIISFSPNTEGFSRAALPFGLVRRELSCEGYSIDLRCPGSDVIMIESANYGRTDDK
  ICDADPFQMENTDCYLPDAFKIMTQRCNNRTQCVVVTGSDVFPDPCPGTYKYLEVQYECVPYMEQKVFVC
  PGTLKAIVDSPSIYEAEQKAGAWCKDPLQAADKIYFMPWTPYRTDTLIEYASLEDFQNSRQTTTYKLPNR
  VDGTGFVVYDGAVFFNKERTRNIVKFDLRTRIKSGEAIINYANYHDTSPYRWGGKTDIDLAVDENGLWVI
  YATEQNNGMIVISQLNPYTLRFEATWETTYDKRAASNAFMICGVLYVVRSVYQDNESEAGKNVIDYIYNT
  RLSRGEHVDVPFPNQYQYIAAVDYNPRDNQLYVWNNNFILRYSLEFGPPDPAQVPTTAVTITSSAELFKT
  TVSTTSSTSQRGPVSSTVAGPQEGSRGTKPPPAVSTTKIPPVTNIFPLPERFCEALEMKGIKWPQTQRGM
  MVERPCPKGTRGTASYLCMASTGTWNPKGPDLSNCTSHWVNQLAQKIRSGENAASLANELAKHTKGTVFA
  GDVSSSVRLMEQLVDILDAQLQELKPSEKDSAGRSYNKLQKREKTCRAYLKAIVDTVDNLLRAETLDCWK
  HMNSSEQAHTATMLLDTLEEGAFVLADNLLEPTRVSMPTDNIVLEVAVLSTEGQVQDFTFHLGFKGAFSS
  IQLSANTVKQNSRNGLAKVVFIIYRSLGPFLSTENATVKLGADLLGRNSTIAVNSHVLSVSINKESSRVY
  LTDPVLFSMPHIDSDNYFNANCSFWNYSERTMMGYWSTQGCKLVDTNKTRTTCACSHLTNFAILMAHREI
  VYKDGVHKLLLTVITWVGIVVSLVCLAICIFTFCFFRGLQSDRNTIHKNLCINLFIAEFIFLIGIDKTQY
  TIACPVFAGLLHFFFLAAFSWMCLEGVQLYLMLVEVFESEYSRKKYYYVAGYLFPATVVGVSAAIDYKSY
  GTLEACWLHVDNYFIWSFIGPVTFIILLNIIFLVITLCKMVKHSNTLKPDSSRLENINNYRVCDGYYNTD
  LPGSWVLGAFALLCLLGLTWSFGLLFVNEETVVMAYLFTAFNAFQGLFIFIFHCALQKKVRKEYAKCFRH
  WYCCGGLPTESPHSSVKASTSRTSARYSSGTQSRIRRMWNDTVRKQSESSFISGMTGNYLLTNPLLRPHG
  TNNPYNTLLAETVVCNAPSAPVFNSPGHSLNNTRDTSAMDTLPLNGNFNNSYSLRKADYHDGVQVVDCGL
  SLNDTAFEKMIISELVHNNLRGSNKTHNLELKLPVKPVIGGSSSEDDAIVADASSLMHGDNPGLEFRHKE
  LEAPLIPQRTHSLLYQPQKKVKPEATDSYVSQLTAEADEHLQSPNRDSLYTSMPNLRDSPYPESSPDMAE
  DLSPSRRSENEDIYYKSMPNLGAGRQLQMCYQISRGNSDGYIIPINKEGCIPEGDVREGQMQLVTSL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001302208
• ORF Size: 4401 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001302208.1, NP_001289137.1
• RefSeq Size: 6222 bp
• RefSeq ORF: 4404 bp
• Locus ID: 171447
• UniProt ID: O88923
• Cytogenetics: 2q45
• MW: 164.5 kDa
• Gene Summary: low affinity G-protein-coupled receptor for alpha-latrotoxin [RGD, Feb 2006]