Rasgrf1 (NM_001170531) Rat Tagged ORF Clone

CAT#: RR217561

Rasgrf1 (myc-DDK-tagged) - Rat RAS protein-specific guanine nucleotide-releasing factor 1 (Rasgrf1), transcript variant 1

Product Images

Circular map for RR217561

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Rasgrf1
• Synonyms: GNRP; P140 RAS-GRF
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR217561 representing NM_001170531
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGAAAGCCATCCGACTGAACGATGGCCACGTCGTGTCCCTGGGACTGCTGGCCCAGAGAGACGGCA
  CGCGCAAAGGCTACCTGAGCAAGAGGAGTTCGGACAACCCAAAATGGCAAACCAAGTGGTTTGCGCTGCT
  GCAGAACCTGCTCTTCTACTTCGAAAGTGACTCGAGCTCTCGGCCCTCGGGGCTCTACCTGCTGGAGGGC
  AGTATCTGCAAACGCATGCCCTCCCCCAAGCGAGGGACCTCCTCCAAGGAGTCCGACAAACAGCATCATT
  ACTTCACAGTGAACTTCTCCAATGACAGCCAGAAGTCCCTAGAGCTGAGGACCGATGACTCCAAGGACTG
  TGACGAGTGGGTGGCAGCGATTGCTCGCGCCAGCTACAAGATACTGGCCACAGAGCATGAGGCGCTCATG
  CAGAAGTACCTGCACCTGCTGCAGGTGGTGGAGACAGAGAAGACCGTGGCTAAGCAGCTGCGACAGCAGC
  TCGAGGATGGCGAGGTCGAGATCGAGCGCCTGAAGGCAGAGATTGCAAACCTGATCAAGGACAATGAACG
  TATCCAGTCCAACCAGCTGGTTGCCCCTGAGGATGAGGACAGTGACATCAAGAAAATTAAGAAGGTACAG
  AGTTTCCTTCGCGGATGGCTGTGCCGGCGAAAGTGGAAGAACATCATCCAGGACTACATCCGGTCTCCTC
  ATGCCGACAGCATGCGCAAGAGGAACCAGGTGGTGTTCAGCATGCTGGAAGCTGAGGCCGAGTACGTGCA
  GCAACTACACATCCTTGTCAACAATTTTCTGCGCCCACTGCGCATGGCCGCCAGCTCTAAGAAACCCCCT
  ATAACACATGACGACGTCAGCAGTATCTTTCTGAACAGTGAGACCATCATGTTCCTGCACCAGATCTTCT
  ACCAAGGCCTGAAGGCCCGTATCGCCAGCTGGCCCACCCTGGTTCTGGCGGACCTGTTCGACATCCTGCT
  GCCAATGCTTAACATCTACCAGGAGTTCGTCCGCAACCACCAGTACAGTCTCCAGATCCTAGCACACTGC
  AAGCAAAACCGGGACTTTGACAAGCTCCTCAAGCAGTATGAGGCCAAGCCAGACTGCGAGGAGCGCACAC
  TGGAGACCTTCCTCACCTATCCAATGTTCCAGATCCCCAGGTACATCCTGACACTCCATGAGCTGCTGGC
  CCACACACCTCATGAGCATGTGGAGCGCAACAGCCTGGACTATGCCAAATCCAAACTAGAGGAGCTGTCC
  AGGGTCATGCACGACGAAGTCAGTGAGACCGAGAACATCCGCAAAAACCTGGCCATTGAGCGTATGATCA
  CCGAGGGCTGTGAGATCCTCCTTGACACCAGCCAGACCTTTGTGCGCCAAGGTTCCCTCATCCAGGTGCC
  CATGTCAGAAAAGGGCAAGATCAACAAGGGCCGCCTGGGGTCTCTGTCCCTTAAGAAAGAAGGTGAGCGC
  CAGTGTTTCCTGTTCTCCAAGCATCTCATCATCTGCACCAGAGGCTCTGGTAGCAAACTGCACCTAACCA
  AGAATGGCGTGATTTCCCTCATTGACTGCACTCTACTGGATGATCCAGAAAACATGGATGATGACGGCAA
  AGGACAAGAGGTAGATCACCTGGACTTTAAGATTTGGGTGGAGCCAAAGGATTCCCCACCCTTCACAGTC
  ATCCTGGTGGCCTCATCCAGGCAGGAGAAGGCGGCATGGACCAGTGACATCATCCAGTGCGTGGATAATA
  TCCGCTGCAACGGGCTCATGATGAATGCCTTTGAAGAAAATTCCAAGGTCACCGTGCCGCAGATGATCAA
  GTCTGATGCTTCCTTATACTGTGATGATGTTGACATTCGCTTCAGCAAAACCATGAATTCTTGCAAAGTG
  CTGCAGATCCGCTATGCCAGCGTGGAGCGCCTGCTGGAGCGCCTGACTGATCTTCGCTTCCTGAGTATTG
  ACTTTCTCAACACCTTCCTGCACTCCTATCGAGTCTTCACCGATGCTGTGGTGGTCCTAGACAAGCTGAT
  CAGCATCTACAAAAAGCCCATCACTGCGATTCCTGCCAGGTCACTGGAACTCCTGTTCTCCAGTAGCCAC
  AACACCAAACTTCTGTACGGAGATGCCCCCAAGTCGCCTCGTGCCAGCCGCAAGTTCTCCTCGCCGCCGC
  CCTTGGCCATCGGCACTTCGTCCCCAGTCCGCCGCCGGAAGTTGTCTCTCAACATTCCCATCATCACAGG
  CGGCAAGGCGCTGGAACTGGCTTCGCTCGGGTGCCCCTCCGACGGCTACACCAACATACACTCGCCCATA
  TCTCCCTTCGGCAAAACCACGCTGGACACCAGCAAGCTCTGTGTGGCCAGCAGCTTGACCAGAACGCCGG
  AGGAGATTGATATGACCACTCTAGAGGAGTCATCAGGCTTCAGGAAGCCGACCTCAGACATCTTGAAAGA
  AGAGTCTGATGATGACCAGAGTGATGTAGACGACACAGAAGTGTCTCCACCAACACCGAAATCATTCAGA
  AACAGAATCACTCAAGAGTTCCCACTCTTTAACTACAACAGTGGAATCATGATGACATGTCGCGATCTGA
  TGGACAGTAACCGCAGCCCTCTGTCAGCTACCTCTGCCTTTGCCATAGCGACTGCAGGAGCCAATGAAAG
  CCCCGCAAACAAGGAGATATATCGAAGGATGTCTTTGGCCAACACAGGGTATTCCTCTGACCAGAGAAAT
  ATCGACAAAGAGTTCGTGATCCGCAGAGCGGCCACCAACCGTGTACTGAATGTGTTGCGCCACTGGGTCA
  CCAAGCACTCCCAGGACTTTGAAACTGACGACCTCCTCAAATACAAGGTGATCTGCTTTCTGGAAGAGGT
  CATGCATGACCCAGACCTTCTACCACAAGAGCGAAAGGCAGCAGCCAACATCATGAGGACTCTGACCCAG
  GAAGAAATAACTGAAAACCATAGCATGCTGGATGAGCTCTTACTAATGACGGAGGGTGTGAAGACTGAGC
  CCTTCGAAAACCACTCAGCCATGGAGATAGCAGAGCAGCTGACCCTGCTGGATCACCTTGTCTTCAAGAG
  TATTCCTTATGAGGAATTCTTTGGCCAGGGCTGGATGAAGGCAGATAAGAATGAAAGGACACCTTACATT
  ATGAAAACCACCAGACATTTCAACCATATCAGTAACTTGATCGCTTCAGAAATTCTCCGAAACGAGGAGG
  TCAGTGCAAGGGCAAGCACCATCGAGAAGTGGGTGGCTGTTGCCGACATTTGCCGCTGCCTGCACAACTA
  CAATGCTGTGCTGGAGATCACTTCCTCCATCAACCGCAGCGCAATCTTCCGACTCAAGAAGACATGGCTC
  AAAGTTTCTAAGCAGACGAAATCTCTGTTTGACAAGCTCCAAAAGCTTGTGTCATCAGATGGCCGATTTA
  AGAACCTCAGAGAAACTTTGCGAAATTGTGATCCACCCTGTGTCCCTTACCTGGGGATGTACCTGACCGA
  CTTGGCATTCCTCGAGGAAGGAACACCCAATTACACAGAGGACGGCCTGGTCAACTTCTCCAAGATGAGG
  ATGATCTCCCATATTATCCGCGAGATTCGCCAGTTTCAGCAGACTACTTACAAAATCGAGCCCCAGCCAA
  AGGTAACTCAGTACTTAGTGGATGAAACCTTTGTGTTGGACGACGAAAGTCTGTATGAGGCCTCCCTCCG
  AATTGAACCAAAACTCCCCACA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR217561 representing NM_001170531
  Red=Cloning site Green=Tags(s)
  
  MQKAIRLNDGHVVSLGLLAQRDGTRKGYLSKRSSDNPKWQTKWFALLQNLLFYFESDSSSRPSGLYLLEG
  SICKRMPSPKRGTSSKESDKQHHYFTVNFSNDSQKSLELRTDDSKDCDEWVAAIARASYKILATEHEALM
  QKYLHLLQVVETEKTVAKQLRQQLEDGEVEIERLKAEIANLIKDNERIQSNQLVAPEDEDSDIKKIKKVQ
  SFLRGWLCRRKWKNIIQDYIRSPHADSMRKRNQVVFSMLEAEAEYVQQLHILVNNFLRPLRMAASSKKPP
  ITHDDVSSIFLNSETIMFLHQIFYQGLKARIASWPTLVLADLFDILLPMLNIYQEFVRNHQYSLQILAHC
  KQNRDFDKLLKQYEAKPDCEERTLETFLTYPMFQIPRYILTLHELLAHTPHEHVERNSLDYAKSKLEELS
  RVMHDEVSETENIRKNLAIERMITEGCEILLDTSQTFVRQGSLIQVPMSEKGKINKGRLGSLSLKKEGER
  QCFLFSKHLIICTRGSGSKLHLTKNGVISLIDCTLLDDPENMDDDGKGQEVDHLDFKIWVEPKDSPPFTV
  ILVASSRQEKAAWTSDIIQCVDNIRCNGLMMNAFEENSKVTVPQMIKSDASLYCDDVDIRFSKTMNSCKV
  LQIRYASVERLLERLTDLRFLSIDFLNTFLHSYRVFTDAVVVLDKLISIYKKPITAIPARSLELLFSSSH
  NTKLLYGDAPKSPRASRKFSSPPPLAIGTSSPVRRRKLSLNIPIITGGKALELASLGCPSDGYTNIHSPI
  SPFGKTTLDTSKLCVASSLTRTPEEIDMTTLEESSGFRKPTSDILKEESDDDQSDVDDTEVSPPTPKSFR
  NRITQEFPLFNYNSGIMMTCRDLMDSNRSPLSATSAFAIATAGANESPANKEIYRRMSLANTGYSSDQRN
  IDKEFVIRRAATNRVLNVLRHWVTKHSQDFETDDLLKYKVICFLEEVMHDPDLLPQERKAAANIMRTLTQ
  EEITENHSMLDELLLMTEGVKTEPFENHSAMEIAEQLTLLDHLVFKSIPYEEFFGQGWMKADKNERTPYI
  MKTTRHFNHISNLIASEILRNEEVSARASTIEKWVAVADICRCLHNYNAVLEITSSINRSAIFRLKKTWL
  KVSKQTKSLFDKLQKLVSSDGRFKNLRETLRNCDPPCVPYLGMYLTDLAFLEEGTPNYTEDGLVNFSKMR
  MISHIIREIRQFQQTTYKIEPQPKVTQYLVDETFVLDDESLYEASLRIEPKLPT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001170531
• ORF Size: 3732 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001170531.1, NP_001164002.1
• RefSeq Size: 4226 bp
• RefSeq ORF: 3735 bp
• Locus ID: 192213
• UniProt ID: P28818
• Cytogenetics: 8q31
• MW: 142.7 kDa
• Gene Summary: a paternally expressed imprinted gene [RGD, Feb 2006]