Nrip1 (NM_001100560) Rat Tagged ORF Clone

CAT#: RR217516

Nrip1 (myc-DDK-tagged) - Rat nuclear receptor interacting protein 1 (Nrip1)

Product Images

Circular map for RR217516

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Nrip1
• Synonyms: RIP140
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR217516 representing NM_001100560
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACTCATGGAGAAGAGCTTGGCTCTGATGTGCATCCGGATTCTATTGTTTTAACTTACCTAGAAGGAT
  TACTAATGCATCAGGCAGCAGGGGGATCAGGTACTGCCGTCGACAAAAAGTCTGCTGGCCACAGCGAAGA
  AGACCAGAACTTTAACCTCTCTGGCAGTGCCTTTCCCACCTGTCAAAGCAATGGTCCCACTCTCAGTACA
  CAGACATACCAGGGATCTGGCATGCTGCACCTCAAAAAAGCCAGACTGCTGCAGTCTTCTGAGGACTGGA
  ACGCGGCTCAGCGGAAGAGGCTGTCCGATTCCATCGTGAATTTAAACGTAAAGAAGGAGGCTTTGCTGGT
  TGGCATGGTTGACAGTGTGCCTAAAGGCAAACAGGATAGCACAGTACTGGCCTCTTTGCTCCAGTCATTC
  AGCTCCAGGCTGCAGACTGTTGCTCTGTCACAGCAGATTAGACAGAGCCTCAAGGAGCAAGGATATGCCC
  TCAGCCACGAGTCGTTGAAAGTGGAGAAGGATTTAAGGTGCTACGGCGTGGCCTCAAGTCACTTAAAAAC
  TCTGCTGAAGAAAAGTAAAACTAAGGATCAGAAGTCAGGTACCACCCTCCCTGATGTGACTCCGAACCTT
  ATCAGAGATAGCTTTGTTGAGTCATCACCTCATCATGTCGGACAAAGTGGAACAAAGGTCATGAGTGAGC
  CCTTGTCATGTGCTGCAAGATTACAGGCTGTTGCCAGCATGGTGGAGAAAAGGGCTAGTCCCGCCGCCTC
  CCCCAAGCCCAGTGTTGCCTGCAGCCAGCTAGCGCTGCTCCTTTCCAGTGAGGCCCACCTGCAGCAGTAC
  TCTCGGGAACACGCTCTCAAAACACAGAGTGCACATCAGGCAGCAAGTGAAAGGCTCGCCGCCATGGCCA
  GATTGCAGGAGAACGGTCAGAAGGACATGAGCAGTTTCCAGCTCTCTAAGGGGGTGTCTGGCCATCTCGA
  CGGGCAGGCCAGAACACTGCCACAGTCAAGCAAACTGGCGGCTAGCAAGAATAACGCAGCAGTCTTTCAG
  AATCCAATGTGCGGTGTTCCTTCCTCTCCCAAAAACACAAGCTATAAGAACTCTCTGGAAAGAAACAACA
  TAAAGCAAGCCGCTAATAACAGTTTGCTTTTACATCTTCTCAAAAGCCAGACCATCCCCACGCCGATGAA
  CGGGCACAGCCAGCACGAGAGAGGAAGTAGTTTTGAGAGTAGCACACCCACCACGATCGATGAGTACTCT
  GATAACAATCCTAGCTTCACAGATGACAGCAGTGGGGATGAAAGCTCATACTCCAATTGCGTTCCCATAG
  ACCTGTCCTGCAAACACCGGACCGAGAAACCGGAAACCGAGCGTCCCGTTTCTCTTGAGAACCTAACCCA
  GTCCTTGTTAAACACGTGGGATCCCAAAGTGCCCGGTGTTGACATCAAAGAAGATCAAGATACCTCAACA
  AATTCCAAGCTGAATTCACACCAGAAAGTCACTCTTCTTCAATTGCTGCTTGGCCATAAAACTGAGGAAA
  GTGTTGAAAGGAATGCCAGCCCTCAGGAAGTCCACAGCGATGTGACTAAGTTCAGTCCTCAGAATTACAC
  AAGGACTTCAGTCATTGAGAGCCCCAGCACCAACAGGACTACCCCAGTGAGCACTCCACCGCTGCACACG
  TCCAGTCAAGGAGAATCTCCCATCAATCTTTCTCAACACTCTCTGGTCATCAAGTGGAATTCCCCGCCAT
  ATGCCTGCAGTACTCCCGCTTCAAAACTAACAAATACCGTGACTGGCCACCTGATGGACCTCACAAAAGG
  CAAAGAATCACAAGCAGAGAAACCAGCCCCGAGTGAAGGTGCACAAAATTCGGCGACGTTCAGCGCCAGC
  AAACTGTTACAGAATTTGGCTCAGTGCGGAATGCAGTCTTCCGTGCCAGGGGAAGAGCAGAGACCCAGCA
  AACAGCTGTTAAGTGGACACACAGACAAACCTCTCGGTCTGATCGATAGGTTAAATAGCCCTCTGCTCTC
  AAATAAAGCAAATGCAGCTGAAGAAAACAAAGCCTTCAGCAGTCAGCCTGCAGGGCCTGAACCAGGACTT
  CCTGGTTGTGAGATAGAAAACCTACTGGAAAGACGCACTGTCCTTCAGTTGCTCCTGGGAAATTCCAACA
  AAGGGAAGAGTGAGAAGAAAGAGAGAGCTCCCGCACTGGACGAGGCTCGTCAGGAGCATTCGGAGAGACC
  CGGAAATGAACAGATACTGATGGTGAAAATTAAATCTGAGCCCTGTGATGACTTCCAGACCCACAACACA
  AACGTGCATTTAAACCACGATGCCAAGAGCACCCCGTTCTTAGGCGGGACTCCCACCATCCAGAGGAGCT
  CGGCGGCCTTACCAGTGTCAGAGGACTTGAAATCAGAGCCTGCTTCACCTCAGGATTTTTCTTTCTCAAA
  GAACGGTCTGTTAAGTCGACTGCTGAGACAGAATCAAGAGAGTTACCCAGCAGATGATCAGGACAAGAGT
  CACAGAAACAGTGAGCTGACACCCCTGGAATCCAAGAACTTTTGCATGGTCCCTAAAAAAAGGAAGCTTT
  ATACGGAACCACTAGAAAACCCATTTAAAAAGATGAAAAATAGTGCCGTAGATAATGCCAATAATCATGG
  TGGCCCCGAAGTACTCTATGGGTCGCTGCTTAATCAGGAGGAGCTGAAATTTAGCAGAAATGATCTCGAC
  TATAAATACTCTGCTGGTCACAGTTCAGCCAGCGATGGTGAACATAGGAGTTGGGCCAGAGAGAGCAAAA
  GCTTCAACGTTCTTAAGCAGCTGCTTCTCTCAGAGAACTGTGTGCGAGATTTGTCCCCACACAGGAGTAA
  CTCTGTCACCGACAATAAAAAGAAAGGACACAAAAACAACGTGCCCAATAGCAAACCTGAATTCAGCATT
  TCTTCTTTAAATGGACTGATGTATGGTTCCCCTCAGCCTGGCAGTTGTGTGGATCATAGGACATTTTCAT
  ACCCAGGAATGGTAAAAACCCCTCTGAGCTCTCCTTTCCCAGAGCACTTGGGCTGTGTAGGGTCCAGGCC
  AGAACCTGGGCTTTTGAATGGCTGTTCTGTGCCCGTGCCCGGTGAGAAGGGACCCATTAAGTGGGTCATC
  ACAGATGTGGAGAAGAATGAGTATGAAAAAGACTCTCCGAGACTGACCAAAACCAACCCAATCCTCTATT
  ACATGCTCCAGAAAGGAGGCAACTCTGTTACCACACAAGAAACACAGGACAAAGACATCTGGGGGGAGCC
  TTCATCTGCCGAGAGTCTCTCACAGGTTACAGTCAAGGAAGAGATGCTTCCTGCCGCAGAAACTAAAGCT
  GCTTTCTTCAATCTCAGAAGCCCTTACAACAGCCATATGGGAAATAATGCTTCCCGCCCGCACAGTACAA
  ACGGAGAAGCGTATGGACCCCTGGGAAACGTGCTCACCATAAAAAAAGAGTGCGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR217516 representing NM_001100560
  Red=Cloning site Green=Tags(s)
  
  MTHGEELGSDVHPDSIVLTYLEGLLMHQAAGGSGTAVDKKSAGHSEEDQNFNLSGSAFPTCQSNGPTLST
  QTYQGSGMLHLKKARLLQSSEDWNAAQRKRLSDSIVNLNVKKEALLVGMVDSVPKGKQDSTVLASLLQSF
  SSRLQTVALSQQIRQSLKEQGYALSHESLKVEKDLRCYGVASSHLKTLLKKSKTKDQKSGTTLPDVTPNL
  IRDSFVESSPHHVGQSGTKVMSEPLSCAARLQAVASMVEKRASPAASPKPSVACSQLALLLSSEAHLQQY
  SREHALKTQSAHQAASERLAAMARLQENGQKDMSSFQLSKGVSGHLDGQARTLPQSSKLAASKNNAAVFQ
  NPMCGVPSSPKNTSYKNSLERNNIKQAANNSLLLHLLKSQTIPTPMNGHSQHERGSSFESSTPTTIDEYS
  DNNPSFTDDSSGDESSYSNCVPIDLSCKHRTEKPETERPVSLENLTQSLLNTWDPKVPGVDIKEDQDTST
  NSKLNSHQKVTLLQLLLGHKTEESVERNASPQEVHSDVTKFSPQNYTRTSVIESPSTNRTTPVSTPPLHT
  SSQGESPINLSQHSLVIKWNSPPYACSTPASKLTNTVTGHLMDLTKGKESQAEKPAPSEGAQNSATFSAS
  KLLQNLAQCGMQSSVPGEEQRPSKQLLSGHTDKPLGLIDRLNSPLLSNKANAAEENKAFSSQPAGPEPGL
  PGCEIENLLERRTVLQLLLGNSNKGKSEKKERAPALDEARQEHSERPGNEQILMVKIKSEPCDDFQTHNT
  NVHLNHDAKSTPFLGGTPTIQRSSAALPVSEDLKSEPASPQDFSFSKNGLLSRLLRQNQESYPADDQDKS
  HRNSELTPLESKNFCMVPKKRKLYTEPLENPFKKMKNSAVDNANNHGGPEVLYGSLLNQEELKFSRNDLD
  YKYSAGHSSASDGEHRSWARESKSFNVLKQLLLSENCVRDLSPHRSNSVTDNKKKGHKNNVPNSKPEFSI
  SSLNGLMYGSPQPGSCVDHRTFSYPGMVKTPLSSPFPEHLGCVGSRPEPGLLNGCSVPVPGEKGPIKWVI
  TDVEKNEYEKDSPRLTKTNPILYYMLQKGGNSVTTQETQDKDIWGEPSSAESLSQVTVKEEMLPAAETKA
  AFFNLRSPYNSHMGNNASRPHSTNGEAYGPLGNVLTIKKECE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001100560
• ORF Size: 3486 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001100560.1, NP_001094030.1
• RefSeq Size: 7910 bp
• RefSeq ORF: 3489 bp
• Locus ID: 304157
• Cytogenetics: 11p11
• MW: 126.7 kDa