Gli1 (NM_001191910) Rat Tagged ORF Clone

CAT#: RR217480

Gli1 (myc-DDK-tagged) - Rat GLI family zinc finger 1 (Gli1)

Product Images

Circular map for RR217480

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Gli1
• Synonyms: Gli
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR217480 representing NM_001191910
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTCAATCCAATGACTTCACCACAAGTCAATAGCTATGGTGAGCCATGCTGTCTCCGACCCCTCCCCA
  GCCAAGGGGTCCCCAGCATGGGAACAGAAGGACTTTCTGGTCTGCCCTTTTGCCACCAAGCCAATTTTAT
  GTCAGGGTCCCAGGGTTATGGGTCTGCCAGAGAGACCAGCAGCTGCACTGAGGGGTCACTCTTTCCTCCT
  CCTCGGAGTTCAGTCAAACTGACTAAGAAGCGGGCTCTTTCCATCTCGCCTCTCTCTGACGCCAGCCTCG
  ACCTGCAAACCGTAATCCGTACCTCACCCAGCTCCCTCGTGGCTTTCATCAACTCTCGTTGTACATCTCC
  AGGCGGTTCCTACGGCCATCTCTCCATTGGCACCATGAGCCCTTCTTTAGGATTCCCACCTCAGATGAGT
  CACCAGAAAGGAACTTCACCTCCCTATGGAGTCCAGCCCTGTGTTCCACATGATTCTACTCAGGGTTCAA
  TGATGCTTCACCCCCAGTCCCGAGGACCACGTGCAACCTGCCAGCTGAAGTCAGAGCTGGACATCATGGT
  TGGCAAGTGCCCAGAGGAACCTTTGGAAGGGGACATGTCTAGCCCCAACTCCACGAGCACACAGGATCAC
  CTGCTGGGGATGCTGGATGGACGGGAGGACCTGGAGAGAGAGGAGAAACCTGAGCCTGAGTCTGTGTATG
  AGACGGACTGCCGCTGGGATGGTTGCAGCCAGGAGTTCGATTCCCAAGAACAGCTGGTGCACCACATCAA
  CAGTGAGCACATCCACGGGGAGCGGAAGGAGTTCGTGTGCCACTGGGGAGGCTGCTCCAGGGAGCTGAGG
  CCCTTCAAGGCCCAGTACATGCTGGTGGTGCACATGCGCAGACACACGGGCGAGAAGCCACACAAGTGCA
  CGTTTGAAGGCTGTCGGAAGTCCTATTCACGCCTCGAAAACCTGAAGACACACCTTCGGTCGCACACCGG
  TGAGAAGCCTTATATGTGTGAGCAGGAAGGCTGCAGCAAGGCCTTCAGCAACGCCAGTGACCGTGCCAAG
  CACCAGAATAGGACCCACTCCAATGAGAAGCCATATGTGTGCAAACTCCCCGGCTGCACCAAGCGCTACA
  CAGATCCCAGCTCGCTCCGCAAACATGTCAAGACTGTGCACGGTCCGGATGCCCACGTGACCAAACGACA
  TCGAGGGGATGGCCCCTTACCACGGGCACAGCCCCTCTCCACAGTGGAGCCCAAGCGAGAAAGGGAAGGA
  GGATCCGGCAGGGAAGAGAGCAGACTGACTGTGCCGGAGGGCACCATGCCTCAGCAGAGCCCTGGAGCAC
  AATCCTCCTGCAGCAGTGACCACTCCCCAGCAGGCAGCGCGGCCAACACGGACAGCGGCGTGGAGATGGC
  CGGCAACGCAGGGGGCAGCACTGAGGACTTGTCCAGCTTGGATGAAGGACCTTGCGTTGCGGCTACCGGA
  CTCTCCACCCTTCGCCGCCTTGAGAACCTCAGGCTGGATCAGCTGCATCAGCTCCGGCCAATAGGGTCCC
  GGGGTCTCAAACTGCCCAGCTTAACCCACGCTGGCGCACCTGTGTCTCGACGTCTGGGCCCACCGGTCTC
  CCTTGACCGCCGCAGCAGCAGCTCCAGCAGCATTAGCTCCGCGTACACAGTCAGCCGCCGGTCCTCGCTG
  GCATCCCCGTTCCCGCCGGGATCCCCACCAGAGAACGGGGCGTCGTCACTACCTGGCCTCACACCTGCCC
  AGCACTACATGCTCCGGGCAAGATATGCTTCAGCCAGGGGGAGTGGCACCCCACCCACTGCAGCACACAG
  CCTGGATCGGATCGGGGGTCTTCCTGCACCTCCTTGGAGAAGCCGAGCTGAGTACCCAGGATACAACCCC
  AATGCAGGGGTCACGAGGAGGGCCAGTGACCCAGCCCGGGCTGCTGACCACCCGGCTCCAGCTAGAGTAC
  AACGGTTCAAGAGCTTGGGGTGTGTACACACGCCTCCTAGTGTGGCAACAGGACGGAACTTCGATCCCCA
  CCTCCCTACCTCTGTCTATTCGCCACAGCCCCCCAGCATCACCGAAAATGTTGCTATGGATACTAGAGGG
  CTACAGGGGGAGCTAGAGGTTGGAACTTCCATGATGGGCAATGATCTGAACCCATACATGGACTTTTCTT
  CTGCTGACACTCTGGGATATGGGGGGCCTGAGGGGACGGCGGCTGAGTCTTATGGATCTAAGGGTCCAGG
  TTCCCTGCCTCTTGGGCCTGGTCCACCAACCAACTATGGCCCTGGCCACTGTGCCCAGCAGGTCTCCTAT
  CCTGATCCCACCCCAGAAACATGGGGTGAGTTTCCTTCACATGCTGGGGTGTACCCTGGCACTAAGGCTC
  TAGGTACAGCCTATAACCAGTGTCCTCGACTTGAGCATTATGGACAAGTGCAAGTAAAACCAGAACAAGG
  GTGCCCAGTGGGGTCTGACTCCACCGGGTTGGCACCCTGCCTCAATGCCCACCCCAGTGAAGGGTCCCCA
  GACCCACAGCCTCTGTTTTCACATCACTCCCAGCTCCCTCCACCCCAGTATCTCCAGTCAGGTCCCTACC
  CTCAGCCTCCCCATGACTGTCTCTCGTTAGAATCCAGGCCTGGCCTCAATTTCAACCCCTCCTCTCATTC
  CACAGGACAGCTCAAAGCTCAGCTCCTGTGTAATTACGTTCAGTCGCAGCAGGAATTGTTGTGGGAGGGC
  AGAAGCCGGGGAGGGATCCCCAACCAGGAACTTCCATATCAGAGCCCCAAGTTTCTGGGAGGTTCTCAAG
  TTAGTCAGAGCCCCACCAAGGTCCCAGCAGCAGCAGCAGCAGCTACGTTTGGATCTGGCTTTGCATCACC
  TGCTTTGGCCAATCACAAATCAGTCTCCTATCCTGCCCCTTCACCGTGCCATGAAACTTTCACCATGGGA
  GTAAACAGGCCTCCCCATAGGCCAGCAGCACCACCCCGGCTTCTGCCCCCGCTACCACCTTGCTATGGGC
  CCCTCAAGGTGGGAGATGCCAACCCCAGCTGTGGGCATCCTGAAGTGGGCAGGTTAGGAGCAGGCCCTGC
  CTTGTACCCTCCCCCTGAAGGGCAGGTGTGTAACCCTCTGGACTCTCTTGACCTGGACAACACTCAGCTG
  GACTTTGTGGCTATCCTAGATGAAGCTCAAGGGCTGAGCCCTCCTCCTTCCCACGAGCAAGGGGACAGCT
  CTAAAAACACCCCACCTCCCTCCGGGCCCCCCAACATGGCAGTCGGTAACATGAGTGTCTTACTGGGGTC
  TCTGCCTGGAGAGACGCAGTTCCTCAACTCTAGTGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR217480 representing NM_001191910
  Red=Cloning site Green=Tags(s)
  
  MFNPMTSPQVNSYGEPCCLRPLPSQGVPSMGTEGLSGLPFCHQANFMSGSQGYGSARETSSCTEGSLFPP
  PRSSVKLTKKRALSISPLSDASLDLQTVIRTSPSSLVAFINSRCTSPGGSYGHLSIGTMSPSLGFPPQMS
  HQKGTSPPYGVQPCVPHDSTQGSMMLHPQSRGPRATCQLKSELDIMVGKCPEEPLEGDMSSPNSTSTQDH
  LLGMLDGREDLEREEKPEPESVYETDCRWDGCSQEFDSQEQLVHHINSEHIHGERKEFVCHWGGCSRELR
  PFKAQYMLVVHMRRHTGEKPHKCTFEGCRKSYSRLENLKTHLRSHTGEKPYMCEQEGCSKAFSNASDRAK
  HQNRTHSNEKPYVCKLPGCTKRYTDPSSLRKHVKTVHGPDAHVTKRHRGDGPLPRAQPLSTVEPKREREG
  GSGREESRLTVPEGTMPQQSPGAQSSCSSDHSPAGSAANTDSGVEMAGNAGGSTEDLSSLDEGPCVAATG
  LSTLRRLENLRLDQLHQLRPIGSRGLKLPSLTHAGAPVSRRLGPPVSLDRRSSSSSSISSAYTVSRRSSL
  ASPFPPGSPPENGASSLPGLTPAQHYMLRARYASARGSGTPPTAAHSLDRIGGLPAPPWRSRAEYPGYNP
  NAGVTRRASDPARAADHPAPARVQRFKSLGCVHTPPSVATGRNFDPHLPTSVYSPQPPSITENVAMDTRG
  LQGELEVGTSMMGNDLNPYMDFSSADTLGYGGPEGTAAESYGSKGPGSLPLGPGPPTNYGPGHCAQQVSY
  PDPTPETWGEFPSHAGVYPGTKALGTAYNQCPRLEHYGQVQVKPEQGCPVGSDSTGLAPCLNAHPSEGSP
  DPQPLFSHHSQLPPPQYLQSGPYPQPPHDCLSLESRPGLNFNPSSHSTGQLKAQLLCNYVQSQQELLWEG
  RSRGGIPNQELPYQSPKFLGGSQVSQSPTKVPAAAAAATFGSGFASPALANHKSVSYPAPSPCHETFTMG
  VNRPPHRPAAPPRLLPPLPPCYGPLKVGDANPSCGHPEVGRLGAGPALYPPPEGQVCNPLDSLDLDNTQL
  DFVAILDEAQGLSPPPSHEQGDSSKNTPPPSGPPNMAVGNMSVLLGSLPGETQFLNSSA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001191910
• ORF Size: 3327 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001191910.1, NP_001178839.1
• RefSeq Size: 3501 bp
• RefSeq ORF: 3330 bp
• Locus ID: 140589
• Cytogenetics: 7q22
• MW: 118.2 kDa
• Gene Summary: mouse homolog is a zinc finger transcription factor and oncogene; involved in development [RGD, Feb 2006]