Wdr44 (NM_001100823) Rat Tagged ORF Clone

CAT#: RR217317

Wdr44 (myc-DDK-tagged) - Rat WD repeat domain 44 (Wdr44)

Product Images

Circular map for RR217317

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Wdr44
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR217317 representing NM_001100823
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGTCGGAAAGCGACACTGAGGAATTCTATGATGCCCCTGAAGATGTGCACCTGGGGGGCAGCTATC
  CTGTGGGGTCTCCAGGAAAAGTTGGACTTTTATCATTCAAGGAAACAAAGAATACAGGAAACAAAGCTGG
  AAATGAGTCCCCTGTACAAGAATTGAGACAAGATGTATCTAAAAAGATAATTGAATGTATTATTGAGGAA
  AGTCAGAAAGTACTACAATTGGAAGATGACTCTTTGGATTCCAAAGGAAAAGGACTTTCTGATCAGGCCA
  CTGCTAGTCATTCTGTGGCTGGGACAGAGTTTAGTAACATACCTGGACTATTAGCCATACAGCATGAACT
  ACAACAAGGTTCCAGAAAGGCAGACAGTCAGAATGCAGCTGAAGAAACTGAACTAGAAACACAAAAATGT
  TTTCCTTCTGATAACAACTGTGAGAAGAGTGAGAAGACAGAAGATGAAACTAATAATTTAACTGAAGTAA
  GTTCAGCTGATCAGCTTGATGCTTCTAAACTAGAAACTGAAATATTGAACAAGGAAGCAGTGGAAGTCAA
  AGAAGGTGATGTTGTAAATCCTGCATCCTCAGATGCTCTATCTACTAAAGATTTTGCTGCTGTGGAAGAA
  GTGGCTCCCGCCAAACCCCCAAGGCACCTTACTCCAGAACCTGATATAGTAGCCAGTACAAAGAAGCCTG
  TTCCTGCACGCCCACCCCCACCAACTAATTTCCCACCTCCTAGACCGCCACCTCCATCTCGACCTGCACC
  ACCACCAAGAAAAAAGAAAAGTGACCTGGAGTTTGAGGCTCTTAAAACACCTGATCTGGATGTACCCAAA
  GACAATATTGCATCTGATTCTCTGCTAACTACAAATATGGCTTCAGAAAGTACAGTTAGAGATTCTCTGC
  CTTCTCTCGATTTAGCAAGTGCTACCAGTGGAGATAAAATAGTTACTGCCCAGGAAAATGGAAAAGCACC
  TGATGTGCAGACAGTAGCAGGTGAAGTGATGGGCCCTCAGAGACCTAGATCCAACTCTGGAAGAGAGCTT
  ACTGATGAGGAAATTTTAGCCAGTGTAATGATTAAGAACCTGGATACTGGAGAAGAAATACCTTTAAGTC
  TTGCTGAAGAGAAGTTGCCCACAGGTATTAATCCTCTCACCCTGCACATCATGAGGAGGACAAAGGAGTA
  TGTAAGCAATGATGCCACACAGTCAGATGATGAAGAAAAGTTACAGTCACAACAAACAGATACTGACGGT
  GGAAGGTTAAAACAGAAAACGACTCAACTAAAGAAATTCCTTGGAAAATCAGTAAAGAGAGCAAAGCATC
  TTGCTGAGGAATATGGTGAACGTGCTATAAATAAAGTTAAAAGTGTAAGAGATGAAGTGTTTCATACTGA
  TCAAGATGACCCTTCATCAAGTGATGATGAAGGAATGCCATATACAAGACCAGTAAAATTCAAGGCAGCG
  CATGGTTTCAAAGGACCTTATGATTTTGATCAGATCAAAGTGGTACAAGATCTTAGTGGTGAACACATGG
  GAGCTGTTTGGACCATGAAATTTTCTCACTGTGGCCGGTTACTTGCCTCAGCTGGACAAGACAATATAGT
  GAGAATATGGGCTTTAAAAAATGCTTTTGACTATTTCAACAATATGCGAATGAAATACAATACTGAAGGA
  CGTGTGTCACCTTCACCATCTCAGGAAAGTCTAAGTTCATCAAAATCAGATACAGACATGGGGGTATGTA
  GTGGAACTGACGAAGACCCAGATGACAAAAATGCACCTTTTCGGCAACGACCATTTTGCAAATATAAGGG
  ACATACTGCTGATCTCCTTGATCTTTCGTGGTCTAAAAACTACTTTCTGCTCTCTTCTTCTATGGATAAA
  ACTGTCAGGTTATGGCACATTTCTAGAAGAGAATGCCTTTGCTGTTTTCAACACATAGATTTTGTCACTG
  CCATCGCTTTCCATCCAAGAGATGACAGATATTTTCTCAGTGGATCATTGGATGGAAAGCTCCGCCTATG
  GAACATACCTGACAAAAAAGTGGCTTTATGGAATGAAGTAGATGGTCAGACAAAGTTGATCACAGCTGCC
  AATTTTTGTCAGAACGGCAAATATGCAGTAATTGGGACATATGATGGTAGATGTATTTTCTATGATACAG
  AGCACTTGAAATACCATACACAAATACATGTACGATCTACTAGAGGGCGGAACAAGGTTGGAAGAAAAAT
  TACTGGGATTGAACCTTTACCTGGGGAAAATAAGATATTGGTAACCTCAAATGACTCCAGAATCAGACTG
  TATGATCTGAGAGACCTATCACTGTCCATGAAGTATAAGGGTTATGTCAATAGCAGCAGCCAGATCAAAG
  CAAGTTTCAGCCATGACTTTACTTACCTCGTTAGCGGTTCAGAAGATAAATATGTTTATATCTGGAGTAC
  TTACCATGACCTAAGCAAGTTTACTTCAGTCAGGAGAGATCGTAATGACTTCTGGGAAGGTATTAAAGCA
  CATAATGCAGTTGTTACGTCAGCCATCTTTGCTCCAAACCCAAGTTTGATGCTATCCTTGGATGTGCAGT
  CTGAAAAATTGGAAGGGATTGATAAATATGAAGATGCTGAAGTTTTGGATAACACATCTACTGGTATCGT
  GAAGACAGATAACACAGAAGTTCTTCTCTCTGCTGATTTTACTGGAGCAATCAAAGTGTTTATTAACAAA
  AGGAAAACTGTATCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR217317 representing NM_001100823
  Red=Cloning site Green=Tags(s)
  
  MASESDTEEFYDAPEDVHLGGSYPVGSPGKVGLLSFKETKNTGNKAGNESPVQELRQDVSKKIIECIIEE
  SQKVLQLEDDSLDSKGKGLSDQATASHSVAGTEFSNIPGLLAIQHELQQGSRKADSQNAAEETELETQKC
  FPSDNNCEKSEKTEDETNNLTEVSSADQLDASKLETEILNKEAVEVKEGDVVNPASSDALSTKDFAAVEE
  VAPAKPPRHLTPEPDIVASTKKPVPARPPPPTNFPPPRPPPPSRPAPPPRKKKSDLEFEALKTPDLDVPK
  DNIASDSLLTTNMASESTVRDSLPSLDLASATSGDKIVTAQENGKAPDVQTVAGEVMGPQRPRSNSGREL
  TDEEILASVMIKNLDTGEEIPLSLAEEKLPTGINPLTLHIMRRTKEYVSNDATQSDDEEKLQSQQTDTDG
  GRLKQKTTQLKKFLGKSVKRAKHLAEEYGERAINKVKSVRDEVFHTDQDDPSSSDDEGMPYTRPVKFKAA
  HGFKGPYDFDQIKVVQDLSGEHMGAVWTMKFSHCGRLLASAGQDNIVRIWALKNAFDYFNNMRMKYNTEG
  RVSPSPSQESLSSSKSDTDMGVCSGTDEDPDDKNAPFRQRPFCKYKGHTADLLDLSWSKNYFLLSSSMDK
  TVRLWHISRRECLCCFQHIDFVTAIAFHPRDDRYFLSGSLDGKLRLWNIPDKKVALWNEVDGQTKLITAA
  NFCQNGKYAVIGTYDGRCIFYDTEHLKYHTQIHVRSTRGRNKVGRKITGIEPLPGENKILVTSNDSRIRL
  YDLRDLSLSMKYKGYVNSSSQIKASFSHDFTYLVSGSEDKYVYIWSTYHDLSKFTSVRRDRNDFWEGIKA
  HNAVVTSAIFAPNPSLMLSLDVQSEKLEGIDKYEDAEVLDNTSTGIVKTDNTEVLLSADFTGAIKVFINK
  RKTVS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001100823
• ORF Size: 2745 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001100823.1, NP_001094293.1
• RefSeq Size: 4020 bp
• RefSeq ORF: 2748 bp
• Locus ID: 246152
• Cytogenetics: Xq34
• MW: 101.6 kDa
• Gene Summary: binds to GTP-Rab11; may be involved in regulation of transferrin accumulation and cell migration [RGD, Feb 2006]