Dag1 (NM_053697) Rat Tagged ORF Clone

CAT#: RR217306

Dag1 (myc-DDK-tagged) - Rat dystroglycan 1 (dystrophin-associated glycoprotein 1) (Dag1)

Product Images

Circular map for RR217306

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Dag1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR217306 representing NM_053697
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTGTGGACAACTGGCTACTGCACCCCCTCTGGGGACAGACCTTTCTCCTCCTACTGTCTGTGGCTG
  TGGCTCAAGCCCACTGGCCCAGTGAACCCTCAGAGGCTGTGAGGGACTGGAAGAACCAGCTTGAGGCGTC
  CATGCACTCAGTTCTCTCGGACTTCCAGGAGGCTGTTCCCACCGTGGTTGGCATCCCAGATGGCACGGCT
  GTTGTTGGGCGCTCATTCCGAGTGAGCATTCCAACAGATTTAATTGCCTCCAGTGGAGAAATTATCAAGG
  TGTCTGCAGCAGGGAAGGAGGCCCTGCCGTCCTGGCTACACTGGGATCCACACAGTCATATTTTGGAAGG
  CCTTCCTCTTGACACTGATAAAGGTGTCCATTACATCTCAGTGAGTGCTGCACGCCTGGGAGCAAATGGA
  AGCCACGTGCCGCAGACGGCCAGCGTGTTCTCTATTGAGGTCTACCCTGAAGACCACAGTGAGCCACAGT
  CTGTCCGGGCAGCCTCATCAGACCCTGGTGAAGTAGTGTCATCTGCTTGTGCAGCTGATGAGCCAGTGAC
  TGTCCTTACAGTGATTCTGGATGCTGACCTCACCAAGATGACCCCAAAGCAAAGGATTGATCTACTGAAC
  AGAATGCAGAGCTTCTCAGAAGTAGAACTTAACAACATGAAGTTGGTGCCTGTAGTCAATAATAGACTAT
  TTGACATGTCAGCCTTCATGGCTGGCCCAGGAAATGCAAAGAAAGTGGTAGAGAATGGGGCTCTGCTGTC
  CTGGAAACTAGGGTGCTCCTTGAATCAGAATAGCGTCCCTGACATCCGCGGTGTAGAAACCCCTGCTAGG
  GAGGGTACCATGTCTGCCCACCTTGGTTATCCTGTGGTGGGTTGGCACATTGCCAATAAGAAGCCCACTC
  TCCCCAAACGAATCCGGAGGCAGATCCATGCCACTCCTACACCTGTTACTGCCATTGGACCCCCAACTAC
  CGCCATTCAGGAGCCACCATCTAGGATAGTGCCTACTCCCACATCTCCAGCCATTGCACCTCCAACAGAG
  ACCATGGCTCCTCCTGTTAGGGATCCTGTCCCAGGGAAGCCCACGGTCACCATTCGGACTCGAGGTGCCA
  TTATTCAGACCCCAACTCTGGGCCCTATCCCTCCTACTCGGGTGTCAGAAGCTGGTACCACAGTTCCTGG
  CCAGATTCGCCCAACACTGACAATTCCTGGTTATGTAGAGCCCACAGCAGTTGTTACTCCTCCAACAACT
  ACCACAAAGAAGCCACGAGTATCTACGCCAAAACCAGCAACGCCTTCAACTGATTCGTCAACTACCACAA
  CTCGAAGGCCAACCAAAAAACCACGGACACCCCGACCAGTGCCCCGGGTCACCACCAAAGCACCGATCAC
  CAGATTGGAGACCGCTTCCCCACCCACTCGAATCCGCACTACCACCAGTGCAGTGCCCCGTGGGGGAGAA
  GCTAACCAGCGGCCAGAGCTTAAGAACCACATCGACAGGGTTGATGCCTGGGTAGGAACCTACTTTGAGG
  TAAAGATTCCATCAGACACCTTCTATGACAATGAGGACACCACTACGGACAAGCTCAAGCTGACCCTGAA
  GCTGCGAGAGCAGCAGTTGGTAGGTGAGAAATCTTGGGTTCAGTTTAACAGCAACAGCCAGCTCATGTAT
  GGTCTGCCTGACAGCAGCCATGTCGGAAAACATGAGTATTTCATGCACGCCACGGACAAAGGGGGCCTTT
  CTGCTGTGGATGCCTTCGAGATCCATGTTCACAAGCGCCCACAAGGGGACAAAGCTCCCGCGCGATTCAA
  GGCCAAACTTGCAGGGGATCCCGCACCTGTGGTGAACGATATCCACAAGAAGATCGCTTTGGTAAAGAAG
  CTGGCTTTTGCCTTTGGGGATAGGAACTGCAGCTCCATCACCCTTCAGAACATCACCCGGGGCTCTATTG
  TGGTCGAGTGGACCAACAACACTCTGCCCCTGGAGCCCTGCCCCAAGGAGCAGATCGTAGGGCTGAGCCG
  CAGGATTGCTGATGAAAACGGGAAGCCTCGTCCTGCCTTCTCCAATGCTCTGGAGCCTGACTTCAAGGCT
  CTGAGTGTTGCTGTGGCGGGCTCTGGCAGTTGTCGGCACCTCCAATTTATCCCCGTGGCACCACCCTCTC
  CTGGAACCTCAGCAGCACCGGCCACAGAGGTTCCAGACAGGGACCCTGAGAAGAGCAGTGAGGATGACGT
  TTACTTGCACACAGTCATCCCAGCTGTTGTGGTTGCAGCCATCCTGCTCATTGCTGGAATCATTGCTATG
  ATCTGCTATCGCAAGAAGAGGAAGGGCAAACTGACCCTGGAGGACCAGGCCACCTTTATCAAGAAGGGGG
  TGCCTATTATCTTTGCAGATGAGCTGGATGACTCTAAGCCCCCGCCCTCTTCCAGCATGCCGCTCATCCT
  GCAGGAAGAGAAGGCTCCCCTCCCTCCTCCTGAGTACCCTAACCAGAGTGTGCCCGAGACCACTCCTCTG
  AACCAGGACACTGTGGGAGAGTACACACCCCTGCGGGATGAGGATCCTAATGCACCTCCCTACCAGCCAC
  CCCCACCCTTCACAGCTCCCATGGAGGGCAAGGGCTCCCGTCCCAAGAACATGACCCCATACCGATCACC
  CCCTCCATATGTACCCCCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR217306 representing NM_053697
  Red=Cloning site Green=Tags(s)
  
  MSVDNWLLHPLWGQTFLLLLSVAVAQAHWPSEPSEAVRDWKNQLEASMHSVLSDFQEAVPTVVGIPDGTA
  VVGRSFRVSIPTDLIASSGEIIKVSAAGKEALPSWLHWDPHSHILEGLPLDTDKGVHYISVSAARLGANG
  SHVPQTASVFSIEVYPEDHSEPQSVRAASSDPGEVVSSACAADEPVTVLTVILDADLTKMTPKQRIDLLN
  RMQSFSEVELNNMKLVPVVNNRLFDMSAFMAGPGNAKKVVENGALLSWKLGCSLNQNSVPDIRGVETPAR
  EGTMSAHLGYPVVGWHIANKKPTLPKRIRRQIHATPTPVTAIGPPTTAIQEPPSRIVPTPTSPAIAPPTE
  TMAPPVRDPVPGKPTVTIRTRGAIIQTPTLGPIPPTRVSEAGTTVPGQIRPTLTIPGYVEPTAVVTPPTT
  TTKKPRVSTPKPATPSTDSSTTTTRRPTKKPRTPRPVPRVTTKAPITRLETASPPTRIRTTTSAVPRGGE
  ANQRPELKNHIDRVDAWVGTYFEVKIPSDTFYDNEDTTTDKLKLTLKLREQQLVGEKSWVQFNSNSQLMY
  GLPDSSHVGKHEYFMHATDKGGLSAVDAFEIHVHKRPQGDKAPARFKAKLAGDPAPVVNDIHKKIALVKK
  LAFAFGDRNCSSITLQNITRGSIVVEWTNNTLPLEPCPKEQIVGLSRRIADENGKPRPAFSNALEPDFKA
  LSVAVAGSGSCRHLQFIPVAPPSPGTSAAPATEVPDRDPEKSSEDDVYLHTVIPAVVVAAILLIAGIIAM
  ICYRKKRKGKLTLEDQATFIKKGVPIIFADELDDSKPPPSSSMPLILQEEKAPLPPPEYPNQSVPETTPL
  NQDTVGEYTPLRDEDPNAPPYQPPPPFTAPMEGKGSRPKNMTPYRSPPPYVPP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_053697
• ORF Size: 2679 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_053697.1, NP_446149.1
• RefSeq Size: 5169 bp
• RefSeq ORF: 2682 bp
• Locus ID: 114489
• Cytogenetics: 8q32
• MW: 96.7 kDa
• Gene Summary: laminin-binding glycoprotein involved in linking the extracellular matrix to the actin cytoskeleton [RGD, Feb 2006]