Mcm3 (NM_001191805) Rat Tagged ORF Clone

CAT#: RR217229

Mcm3 (myc-DDK-tagged) - Rat minichromosome maintenance complex component 3 (Mcm3)

Product Images

Circular map for RR217229

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Mcm3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR217229 representing NM_001191805
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGGTACCGTAGTGCTGGACGACGTGGAGCTGCGCGAAGCGCAGAGAGACTACTTGGACTTCCTGG
  ACGACGAGGAAGACCAAGGAATTTACCAGAATAAAGTTCGGGAGCTGATCAGTGACAACCAGTATCGGCT
  GATTGTCAATGTGAATGACCTGCGCAGGAAAAATGAAAAGAGGGCTAACCGCCTCCTGAACAATGCTTTC
  GAGGAGCTGGTTGCCTTCCAGCGGGCCTTGAAGGATTTCGTGGCCTCCATTGACGCTACCTATGCCAAGC
  AGTACGAGGAGTTCTACATAGGCCTAGAGGGCAGCTTCGGCTCAAAGCACGTCTCTCCCCGGACTCTCAC
  TTCCTGCTTCCTTAGCTGTGTGGTCTGCGTGGAAGGCATCGTTACCAAATGCTCTCTGGTTCGCCCCAAG
  GTCGTTCGCAGCGTTCACTACTGTCCTGCTACTAAGAAGACCATAGAGCGGCGCTACTCTGACCTCACCA
  CCCTGGTGGCTTTCCCATCCAGTTCTGTTTATCCCACAAAGGATGAGGAGAACAACCCCCTGGAGACAGA
  GTATGGCTTGTCTGTCTACAAGGACCACCAGACCATCACCATCCAGGAGATGCCGGAGAAGGCCCCAGCC
  GGTCAGCTTCCTCGATCTGTGGATGTCATTCTAGATGATGATCTGGTAGATAAAGTGAAACCTGGTGACA
  GAATCCAGGTGGTTGGGACCTACCGCTGCCTTCCAGGAAAGAAGGGTGGCTACACCTCGGGGACCTTCAG
  GACCGTCCTGATCGCCTGCAACGTGAAGCAGATGAGCAAGGACATTCAGCCTGCGTTCTCTGCAGACGAT
  ATAGCAAAGATCAAGAAGTTCAGCAAAACCCGTTCCAAGGATGTCTTTGAGCAGTTGGCCCGATCGTTAG
  CCCCTAGCATCCATGGGCATGACTATGTCAAGAAAGCAATCCTCTGCTTGCTCCTGGGAGGGGTGGAACG
  GGAACTTGAAAACGGCAGCCACATCCGTGGAGATATCAATATTCTCCTAATAGGTGACCCATCAGTCGCC
  AAGTCCCAGCTTCTGCGGTATGTGCTTTGCACCGCACCCAGGGCCATTCCCACCACGGGTCGGGGCTCGT
  CTGGAGTGGGTCTCACAGCAGCCGTCACCACAGACCAGGAAACCGGGGAGCGCCGCCTGGAAGCTGGTGC
  CATGGTCCTGGCTGACCGAGGAGTGGTTTGCATCGATGAGTTCGACAAGATGTCGGACATGGACCGCACA
  GCCATCCATGAGGTGATGGAGCAGGGTAGAGTGACGATCGCGAAGGCTGGCATCCATGCCCGGCTCAATG
  CCCGTTGCAGTGTTTTGGCAGCTGCCAATCCAGTCTATGGCAGGTATGACCAGTATAAGACACCTATGGA
  GAACATTGGGCTGCAGGACTCACTGCTGTCTCGGTTTGACCTGCTCTTCATCATGCTGGATCAGATGGAT
  CCTGAACAGGACCGGGAGATCTCAGACCACGTCCTTAGGATGCACCAATACAGGGCCCCGGGGGAGCAGG
  ACGGTGACGCTTTGCCGTTGGGTAGTTCAGTGGATATCCTGGCCACAGACGATCCCGACTTTACCCAGGA
  TGACCAGCAAGATACCCGGATTTATGAGAAGCATGACAACCTTCTACATGGGTCCAAGAAGAAAAGGGAG
  AAGATGGTGAGCGCAGCTTTCATGAAGAAGTACATCCACGTGGCCAAAATTATTAAACCGACCCTGACGC
  AGGAGTCCGCAGCCTACATTGCAGAAGAATACTCTCGCCTGCGCAGCCAGGACAGCATGAGCTCAGACAC
  TGCCCGGACATCTCCGGTTACAGCACGGACACTGGAAACTCTGATTCGACTGGCCACGGCCCATGCGAAG
  GCCCGTATGAGCAAGACTGTGGACCTGCAAGATGCGGAGGAAGCTGTGGAACTGGTGCAGTATGCTTATT
  TCAAGAAGGTTCTGGAAAAGGAGAAGAAACGTAAGAAGCCAAGTGAGGATGAATCAGACCTGGAGGATGA
  AGAGGAGAAGAGCCAGGAGGAGGACACTGAGCAGACTCGGAAGAGGAGGAAGACTCAAGCCAAGGATGGG
  GAGTCCTATGACCCTTATGACTTCAGTGAAGCAGAGAGGGAAATGCCTCAAGTGCACACCCCGAAGACCG
  ACGACTCTCAAGAGAAGACAGATGAGTCTCAGGAGACCCAGGAGTCCCAGAAAGTGGAGCTGAGTGAGTC
  CAGGCTGAAGGCTTTTAAGGCGGCTCTCTTAGAAGTGTTCCAGGAGGCTCACGCGCAGTCTGTGGGCATG
  ATCCACCTCACGGAATCCATCAATCGGAATAAAGAAGAACCCTTCTCCTCAGAGGAAATCCAGGCCTCAC
  TGAGCCGGATGCAGGACGATAACCAGGTCATGGTGTCTGAGGGCATCGTCTTCCTTATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR217229 representing NM_001191805
  Red=Cloning site Green=Tags(s)
  
  MAGTVVLDDVELREAQRDYLDFLDDEEDQGIYQNKVRELISDNQYRLIVNVNDLRRKNEKRANRLLNNAF
  EELVAFQRALKDFVASIDATYAKQYEEFYIGLEGSFGSKHVSPRTLTSCFLSCVVCVEGIVTKCSLVRPK
  VVRSVHYCPATKKTIERRYSDLTTLVAFPSSSVYPTKDEENNPLETEYGLSVYKDHQTITIQEMPEKAPA
  GQLPRSVDVILDDDLVDKVKPGDRIQVVGTYRCLPGKKGGYTSGTFRTVLIACNVKQMSKDIQPAFSADD
  IAKIKKFSKTRSKDVFEQLARSLAPSIHGHDYVKKAILCLLLGGVERELENGSHIRGDINILLIGDPSVA
  KSQLLRYVLCTAPRAIPTTGRGSSGVGLTAAVTTDQETGERRLEAGAMVLADRGVVCIDEFDKMSDMDRT
  AIHEVMEQGRVTIAKAGIHARLNARCSVLAAANPVYGRYDQYKTPMENIGLQDSLLSRFDLLFIMLDQMD
  PEQDREISDHVLRMHQYRAPGEQDGDALPLGSSVDILATDDPDFTQDDQQDTRIYEKHDNLLHGSKKKRE
  KMVSAAFMKKYIHVAKIIKPTLTQESAAYIAEEYSRLRSQDSMSSDTARTSPVTARTLETLIRLATAHAK
  ARMSKTVDLQDAEEAVELVQYAYFKKVLEKEKKRKKPSEDESDLEDEEEKSQEEDTEQTRKRRKTQAKDG
  ESYDPYDFSEAEREMPQVHTPKTDDSQEKTDESQETQESQKVELSESRLKAFKAALLEVFQEAHAQSVGM
  IHLTESINRNKEEPFSSEEIQASLSRMQDDNQVMVSEGIVFLI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001191805
• ORF Size: 2439 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001191805.2, NP_001178734.2
• RefSeq Size: 2932 bp
• RefSeq ORF: 2442 bp
• Locus ID: 316273
• Cytogenetics: 9q13
• MW: 91.7 kDa