Bpifb4 (NM_001109209) Rat Tagged ORF Clone

CAT#: RR217004

Bpifb4 (myc-DDK-tagged) - Rat BPI fold containing family B, member 4 (Bpifb4)

Product Images

Circular map for RR217004

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Bpifb4
• Synonyms: Lplunc4; RY2G5
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR217004 representing NM_001109209
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTGGACTGCTTGGTGTGTGGCAGCTCTGTCAGTGGCAGCTGTGTGTGGCATCCGTCAAGACACAACCA
  CAGTCCTCCGAGTTACAAAAGATGTGCTGGGCGATGCCATCTCAGGCACAATTCAGAAGAGTGACGCCTT
  CCGCTCCGCCCTGAGGGAGGTGCCCGTGGGTGTCGGTGGTGTCCCCTACAACGACTTCCATGTCCGAGAG
  CCTCCCCCCAAATACACCAATGGCAGACAGCTTGGTGGCAATTACAAATACGGTCACATTGAGGCGAATG
  ACAACACAGCTCAGCTGGGGGGCAAATACCGCTATGGGGAGATCCTGGACTCTGATGGGAGCCTCAGGGA
  CCTGCGGCACGAAGACTACCGTCCTTCAGACTCTGCCTACCACCGCGGCCCCGGGAGGTACAGGTCTGCA
  GCGGACCCGTCCTCGGCGGGCAGGCTCCACCGGCGCGAACTGAGGCCTGGAGAGATCCCAGCTGGTGTGG
  CCACTGGAGCACTGGGCCCCGGAGGCCTGCTGGGCACTGGGGGCATACTTGCAAACGAAGGCATTCTGGC
  AGGCCAAGGGGGCTTGCTTGGAGGAGGCGGTCTTCTTGGAGATGGAGGACTTCTCGGAGGAGGTGGTGTG
  CTCGGTGTGCTGGGTGAAGGAGGTATCCTGAGCACCGTCCAAGGCATCACTGGGTTGCGCATCGTGGAAC
  TTACCCTTCCCCGGGTGTCTGTGAGACTCCTGCCCGGTGTGGGTGTCTATCTGAGCTTGTACACTCGTGT
  GGCCATCAATGGAAAGAGCCTCATTGGCTTCCTGGATATTGCAGTGGAGGTTAACATCACCGCCAAAGTT
  CGGCTGACCATGGACCGCACAGGCTACCCGCGGCTGGTCATTGAGCGCTGTGACACCCTCCTGGGAGGCA
  TCAAAGTCAAGCTGCTTCGGGGGCTTCTCCCCAACCTGGTGGATAACTTAGTGAACCGAGTGCTGGCCAA
  CGTACTCCCTGACCTGCTCTGTCCCATTGTGGATGTGGTTTTGGGTCTTGTCAATGACCAGCTGGGTCTC
  GTGGACTCTTTGGTGCCTCTCGGAATACTTGGGAGTGTGCAATACACTTTCTCCAGCCTCCCACTGGTGA
  CCGGGGAGTTCCTTGAGTTGGACCTTAATACTCTAGTTGGGGAGGCAGGAGGTGACCTCATTGACTACCC
  CCTGGGCCGGCCAGCTATGTTGCCCAGGCCACAGATGCCAGAACTACCCCCCATGGGCGACAACACCAAC
  TCCCAGCTGGCCATCTCGGCCAATTTCCTGAGCTCGGTGCTGACCATGCTGCAGAAGCAAGGTGCATTGG
  ACATAGACATCACTGATGGCATGTTTGAAGATCTCCCTCCACTTACCACTTCCACATTGGGGGCCTTGAT
  TCCCAAGGTGTTCCAGCAATACCCTGAGTCCCGCCCACTCACCATCAGGATCCAGGTGCCCAACCCCCCA
  ACTGTGACCCTGCAGAAGGACAAGGCACTGGTGAAGGTGTTTGCCACCTCTGAAGTTGTCGTCTCCCAGC
  CTAATGATGTGGAGACCACCATCTGCCTCATCGATGTGGACACAGACCTCTTGGCTTCATTTTCTGTGGA
  AGGGGATAAGCTCATGATCGATGCCAAGCTGGATAAGACCAGCCTCAACCTCAGAACCTCAAACGTGGGC
  AACTTTGATGTGTTCATCTTGGAAATGTTGGTTGAGAAGATCTTTGACCTAGCGTTTATGCCCGCAATGA
  ATGCTATACTGGGTTCTGGAGTCCCTCTGCCCAAAATCCTCAACATTGACTTCAGCAACGCCGACATCGA
  CGTGTTGGAGGACCTTCTGGTGCTGAGCACA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR217004 representing NM_001109209
  Red=Cloning site Green=Tags(s)
  
  MWTAWCVAALSVAAVCGIRQDTTTVLRVTKDVLGDAISGTIQKSDAFRSALREVPVGVGGVPYNDFHVRE
  PPPKYTNGRQLGGNYKYGHIEANDNTAQLGGKYRYGEILDSDGSLRDLRHEDYRPSDSAYHRGPGRYRSA
  ADPSSAGRLHRRELRPGEIPAGVATGALGPGGLLGTGGILANEGILAGQGGLLGGGGLLGDGGLLGGGGV
  LGVLGEGGILSTVQGITGLRIVELTLPRVSVRLLPGVGVYLSLYTRVAINGKSLIGFLDIAVEVNITAKV
  RLTMDRTGYPRLVIERCDTLLGGIKVKLLRGLLPNLVDNLVNRVLANVLPDLLCPIVDVVLGLVNDQLGL
  VDSLVPLGILGSVQYTFSSLPLVTGEFLELDLNTLVGEAGGDLIDYPLGRPAMLPRPQMPELPPMGDNTN
  SQLAISANFLSSVLTMLQKQGALDIDITDGMFEDLPPLTTSTLGALIPKVFQQYPESRPLTIRIQVPNPP
  TVTLQKDKALVKVFATSEVVVSQPNDVETTICLIDVDTDLLASFSVEGDKLMIDAKLDKTSLNLRTSNVG
  NFDVFILEMLVEKIFDLAFMPAMNAILGSGVPLPKILNIDFSNADIDVLEDLLVLST
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001109209
• ORF Size: 1851 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001109209.2, NP_001102679.2
• RefSeq Size: 2100 bp
• RefSeq ORF: 1854 bp
• Locus ID: 499925
• UniProt ID: Q05704
• Cytogenetics: 3q41
• MW: 65.6 kDa
• Gene Summary: May have the capacity to recognize and bind specific classes of odorants. May act as a carrier molecule, transporting odorants across the mucus layer to access receptor sites. May serve as a primary defense mechanism by recognizing and removing potentially harmful odorants or pathogenic microorganisms from the mucosa or clearing excess odorant from mucus to enable new odorant stimuli to be received (By similarity).[UniProtKB/Swiss-Prot Function]