Nlk (NM_001191924) Rat Tagged ORF Clone

CAT#: RR216856

Nlk (myc-DDK-tagged) - Rat nemo like kinase (Nlk)

Product Images

Circular map for RR216856

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Nlk
• Synonyms: RGD1561602
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR216856 representing NM_001191924
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTCTTTGTGGCACACGAGCCAACGCAAAAATGATGGCGGCTTACAATGGCGGTACATCTGCAGCAG
  CAGCAGGTCACCACCACCACCATCACCACCACCTTCCACACCTTCCTCCTCCTCACCTTCACCACCACCA
  CCACCCTCAACACCACCTTCATCCGGGGTCGGCTGCTGCTGTACACCCTGTACAGCAGCATACGTCTTCG
  GCAGCTGCGGCAGCCGCAGCGGCAGCTGCGGCCGCAGCCATGTTAAACCCTGGGCAACAACAGCCATATT
  TCCCATCACCGGCACCTGGTCAGGCTCCTGGGCCAGCTGCAGCAGCCCCAGCTCAGGTACAGGCTGCTGC
  AGCTGCTACAGTTAAGGCGCACCATCATCAGCACTCGCATCATCCGCAACAGCAGCTGGACATTGAGCCG
  GATAGACCTATTGGATATGGAGCCTTTGGTGTTGTCTGGTCAGTAACAGATCCAAGAGATGGAAAGAGAG
  TAGCACTCAAAAAGATGCCCAACGTCTTCCAGAATCTGGTCTCTTGCAAAAGAGTCTTCCGGGAATTGAA
  GATGTTGTGTTTTTTTAAACATGATAATGTACTCTCTGCCCTTGACATACTCCAGCCTCCACACATTGAC
  TATTTTGAAGAAATATATGTTGTCACAGAATTGATGCAGAGTGATCTACATAAAATTATCGTCTCTCCTC
  AGCCACTCAGCTCAGATCATGTCAAAGTTTTTCTTTATCAGATTTTGCGAGGTTTGAAATATCTCCATTC
  AGCTGGCATTTTACATCGCGACATTAAGCCGGGGAATCTCCTTGTGAACAGCAACTGTGTTCTAAAGATT
  TGTGATTTTGGATTGGCCAGAGTGGAAGAATTGGATGAATCCCGTCACATGACTCAGGAAGTTGTTACTC
  AGTATTACCGGGCTCCGGAGATCCTCATGGGCAGCCGCCATTACAGCAATGCTATTGACATCTGGTCTGT
  GGGGTGCATCTTTGCAGAGCTGCTGGGCCGAAGGATACTGTTTCAGGCGCAGAGTCCCATTCAGCAGTTG
  GATTTGATCACAGATCTGTTGGGCACGCCATCACTGGAAGCGATGAGGACAGCTTGTGAAGGTGCTAAGG
  CACACATACTCAGGGGTCCTCACAAACAGCCATCTCTCCCTGTACTCTACACCCTTTCCAGCCAGGCCAC
  ACATGAAGCCGTTCACCTGCTTTGCAGAATGTTGGTCTTTGATCCATCAAAAAGAATATCCGCTAAGGAT
  GCCTTAGCCCACCCCTACCTAGATGAAGGGCGGCTGAGATACCATACATGTATGTGTAAGTGCTGCTTTT
  CCACCTCGACGGGAAGAGTCTACACCAGTGACTTTGAGCCTGTCACCAACCCCAAATTTGATGACACCTT
  TGAGAAGAACCTCAGCTCTGTCCGACAAGTTAAAGAAATTATCCACCAGTTCATTTTGGAACAGCAGAAA
  GGAAACAGAGTACCTCTCTGCATTAACCCGCAGTCTGCTGCCTTTAAGAGCTTTATCAGTTCCACCGTCG
  CTCAGCCGTCTGAGATGCCACCGTCTCCTCTGGTGTGGGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR216856 representing NM_001191924
  Red=Cloning site Green=Tags(s)
  
  MSLCGTRANAKMMAAYNGGTSAAAAGHHHHHHHHLPHLPPPHLHHHHHPQHHLHPGSAAAVHPVQQHTSS
  AAAAAAAAAAAAAMLNPGQQQPYFPSPAPGQAPGPAAAAPAQVQAAAAATVKAHHHQHSHHPQQQLDIEP
  DRPIGYGAFGVVWSVTDPRDGKRVALKKMPNVFQNLVSCKRVFRELKMLCFFKHDNVLSALDILQPPHID
  YFEEIYVVTELMQSDLHKIIVSPQPLSSDHVKVFLYQILRGLKYLHSAGILHRDIKPGNLLVNSNCVLKI
  CDFGLARVEELDESRHMTQEVVTQYYRAPEILMGSRHYSNAIDIWSVGCIFAELLGRRILFQAQSPIQQL
  DLITDLLGTPSLEAMRTACEGAKAHILRGPHKQPSLPVLYTLSSQATHEAVHLLCRMLVFDPSKRISAKD
  ALAHPYLDEGRLRYHTCMCKCCFSTSTGRVYTSDFEPVTNPKFDDTFEKNLSSVRQVKEIIHQFILEQQK
  GNRVPLCINPQSAAFKSFISSTVAQPSEMPPSPLVWE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001191924
• ORF Size: 1581 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001191924.2
• RefSeq Size: 3601 bp
• RefSeq ORF: 1584 bp
• Locus ID: 497961
• UniProt ID: D3ZSZ3
• Cytogenetics: 10q25
• MW: 58.3 kDa
• Gene Summary: Serine/threonine-protein kinase that regulates a number of transcription factors with key roles in cell fate determination. Positive effector of the non-canonical Wnt signaling pathway, acting downstream of WNT5A, MAP3K7/TAK1 and HIPK2. Activation of this pathway causes binding to and phosphorylation of the histone methyltransferase SETDB1. The NLK-SETDB1 complex subsequently interacts with PPARG, leading to methylation of PPARG target promoters at histone H3K9 and transcriptional silencing. The resulting loss of PPARG target gene transcription inhibits adipogenesis and promotes osteoblastogenesis in mesenchymal stem cells (MSCs). Negative regulator of the canonical Wnt/beta-catenin signaling pathway. Binds to and phosphorylates TCF7L2/TCF4 and LEF1, promoting the dissociation of the TCF7L2/LEF1/beta-catenin complex from DNA, as well as the ubiquitination and subsequent proteolysis of LEF1. Together these effects inhibit the transcriptional activation of canonical Wnt/beta-catenin target genes. Negative regulator of the Notch signaling pathway. Binds to and phosphorylates NOTCH1, thereby preventing the formation of a transcriptionally active ternary complex of NOTCH1, RBPJ/RBPSUH and MAML1. Negative regulator of the MYB family of transcription factors. Phosphorylation of MYB leads to its subsequent proteolysis while phosphorylation of MYBL1 and MYBL2 inhibits their interaction with the coactivator CREBBP. Other transcription factors may also be inhibited by direct phosphorylation of CREBBP itself. Acts downstream of IL6 and MAP3K7/TAK1 to phosphorylate STAT3, which is in turn required for activation of NLK by MAP3K7/TAK1. Upon IL1B stimulus, cooperates with ATF5 to activate the transactivation activity of C/EBP subfamily members. Phosphorylates ATF5 but also stabilizes ATF5 protein levels in a kinase-independent manner.[UniProtKB/Swiss-Prot Function]