Rnh1 (NM_001270762) Rat Tagged ORF Clone

CAT#: RR216783

Rnh1 (myc-DDK-tagged) - Rat ribonuclease/angiogenin inhibitor 1 (Rnh1), transcript variant 1

Product Images

Circular map for RR216783

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Rnh1
• Synonyms: Rnh
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR216783 representing NM_001270762
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTTTGGGGCTCCCGTCTGCCTTTGAGAGTCTTTTTCGGGTCCCTTCCTGGCCTTCTTAATACGTGGC
  AGAGCGTCTGGACCAGAAGACTACACTGTGCACCCACCATGAGTCTTGACATCCAGTGTGAGCAGCTGAG
  TGATGCCCGGTGGACAGAGCTCCTTCCCCTGATCCAACAATACCAAGTGGTCAGGCTGGATGACTGTGGC
  CTCACTGAAGTGCGGTGCAAAGACATCAGGTCAGCGATCCAGGCCAACCCTGCCCTGACAGAGCTCAGCC
  TACGCACCAATGAACTGGGTGATGCTGGTGTGGGTCTGGTGCTCCAGGGCCTGCAGAATCCCACTTGTAA
  GATCCAGAAGCTGAGCCTTCAGAACTGCAGCTTGACGGAAGCTGGCTGTGGGGTCCTGCCTGATGTGCTG
  CGCTCTTTGTCTACCCTGCGTGAACTACATCTCAATGACAACCCTCTGGGGGATGAAGGCCTGAAGCTGC
  TCTGTGAAGGACTCCGGGACCCCCAGTGCCGTCTTGAGAAGCTTCAGTTGGAATACTGTAACCTCACAGC
  TACCAGCTGCGAGCCCCTGGCCTCAGTGCTCAGGGTGAAACCTGACTTTAAAGAGCTAGTATTGAGCAAC
  AATGACTTCCATGAGGCTGGTATCCACACTCTGTGCCAGGGCCTGAAGGATTCTGCCTGTCAACTGGAGT
  CACTCAAACTGGAGAACTGTGGTATCACATCAGCCAACTGCAAGGATCTGTGTGATGTTGTGGCCTCCAA
  AGCCTCACTGCAAGAACTGGACTTGGGCAGCAACAAGCTGGGCAACACAGGCATTGCAGCACTGTGCTCA
  GGACTGCTGCTTCCCAGCTGCAGGCTGAGGACTCTGTGGCTCTGGGACTGTGATGTCACTGCAGAAGGCT
  GCAAGGACCTGTGCCGTGTCCTCAGAGCCAAGCAGAGCCTGAAGGAACTCAGCCTAGCTGGCAATGAGCT
  GAAGGATGAGGGTGCCCAACTGCTGTGTGAGAGCCTGTTAGAGCCTGGCTGTCAGCTGGAGTCACTGTGG
  GTAAAGACCTGTAGCCTCACAGCTGCCTCTTGTCCCCACTTCTGCTCGGTGTTGACCAAAAATCGTTCTC
  TGTTTGAGCTGCAAATGAGCAGCAACCCGCTGGGAGACTCGGGAGTCGTGGAGCTTTGCAAGGCCCTGGG
  CTATCCGGACACAGTGCTGCGTGTGCTTTGGCTGGGAGACTGTGATGTGACAGACAGTGGCTGCAGCAGC
  CTTGCCACTGTCCTGCTGGCCAACCGCAGCTTGAGGGAACTGGACCTCAGTAACAACTGCATGGGGGACA
  CCGGTGTCCTACAACTGCTGGAGAGCCTCAAACAGCCCAGCTGCGCCCTTCAGCAGCTTGTCCTGTATGA
  CATTTACTGGACGGATGAGGTGGAAGACCAGCTTCGGGCCCTGGAGGAGGAAAGGCCATCCCTGAGGATC
  ATTTCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR216783 representing NM_001270762
  Red=Cloning site Green=Tags(s)
  
  MFWGSRLPLRVFFGSLPGLLNTWQSVWTRRLHCAPTMSLDIQCEQLSDARWTELLPLIQQYQVVRLDDCG
  LTEVRCKDIRSAIQANPALTELSLRTNELGDAGVGLVLQGLQNPTCKIQKLSLQNCSLTEAGCGVLPDVL
  RSLSTLRELHLNDNPLGDEGLKLLCEGLRDPQCRLEKLQLEYCNLTATSCEPLASVLRVKPDFKELVLSN
  NDFHEAGIHTLCQGLKDSACQLESLKLENCGITSANCKDLCDVVASKASLQELDLGSNKLGNTGIAALCS
  GLLLPSCRLRTLWLWDCDVTAEGCKDLCRVLRAKQSLKELSLAGNELKDEGAQLLCESLLEPGCQLESLW
  VKTCSLTAASCPHFCSVLTKNRSLFELQMSSNPLGDSGVVELCKALGYPDTVLRVLWLGDCDVTDSGCSS
  LATVLLANRSLRELDLSNNCMGDTGVLQLLESLKQPSCALQQLVLYDIYWTDEVEDQLRALEEERPSLRI
  IS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001270762
• ORF Size: 1476 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001270762.1, NP_001257691.1
• RefSeq Size: 1673 bp
• RefSeq ORF: 1479 bp
• Locus ID: 100360501
• UniProt ID: P29315
• Cytogenetics: 1q41
• MW: 54.1 kDa
• Gene Summary: ribonuclease inhibitor [RGD, Feb 2006]