G3bp1 (NM_133565) Rat Tagged ORF Clone

CAT#: RR216732

G3bp1 (myc-DDK-tagged) - Rat GTPase activating protein (SH3 domain) binding protein 1 (G3bp1)

Product Images

Circular map for RR216732

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: G3bp1
• Synonyms: G3bp; RGD1310666
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR216732 representing NM_133565
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTTATGGAGAAGCCTAGTCCCCTGCTGGTCGGGCGGGAGTTTGTGCGACAGTACTACACCCTGCTGA
  ACCAGGCCCCGGACATGCTGCACAGATTTTATGGCAAGAACTCTTCCTATGCCCACGGGGGCTTGGATTC
  CAACGGGAAGCCAGCAGATGCAGTCTACGGGCAGAAGGAAATCCACAGGAAAGTGATGTCACAAAACTTC
  ACCAACTGCCACACCAAGATCCGCCACGTGGATGCTCACGCAACTCTGAATGACGGGGTGGTGGTCCAAG
  TGATGGGGCTGCTGTCCAACAACAACCAGGCTCTGCGGAGGTTCATGCAGACCTTTGTCCTTGCGCCTGA
  GGGCTCTGTTGCCAACAAATTCTATGTTCACAATGACATCTTCAGATACCAAGATGAGGTCTTCGGTGGC
  TTTGTCACAGAGCCTCAAGAGGAATCTGAGGAAGAAGTAGAGGAACCTGAAGAAAGACAGCAGTCACCAG
  AGGTGGTTGCTGATGACTCTGGAACTTTCTATGATCAGACTGTTAGCAATGACTTGGAGGAACATTTAGA
  GGAGCCTGTTGTGGAACCAGAACCAGAGCCGGAGCCAGAGCCGGAACCGGAACCTGTGTCCGACATTCAA
  GAGGACAAGCCTGAGCCTGCATTGGAAGAAGCCGCTCCTGAGGATGTGCAGAAGAGCGCCTCCCCGGCCC
  CGGCAGACGTGGCCCCAGCACAGGAGGACTTGAGGACATTTTCTTGGGCGTCTGTGACTAGTAAGAACCT
  TCCTCCCAGTGGGGCTGTTCCAGTGACAGGGACACCACCTCATGTGGTCAAAGTGCCAGCATCACAGCCC
  CGTCCAGAATCTAAGCCTGACTCTCAGATCCCACCACAAAGGCCTCAGAGAGATCAGAGAGCTCGAGAGC
  AGCGGATCAATATCCCTCCTCAGAGAGGACCCCGGCCAATTCGTGAGGCTGGTGAACCAGGAGATGTGGA
  ACCCCGAAGGATGGTGAGGCACCCTGACAGTCACCAGCTCTTCATTGGGAACCTGCCACATGAGGTTGAC
  AAGTCGGAACTGAAGGATTTTTTCCAAAGTTATGGGAATGTTGTGGAGCTGCGCATCAACAGTGGTGGGA
  AGTTACCCAACTTCGGTTTCGTTGTGTTTGATGACTCGGAACCAGTTCAGAAGGTCCTTAATAACAGGCC
  CATCATGTTCCGAGGTGCGGTTCGCCTGAATGTGGAAGAGAAGAAGACTCGAGCTGCCAGGGAAGGCGAC
  CGCAGGGATAACCGCCTTCGGGGACCTGGAGGCCCCCGTGGTGGGCCGAGTGGTGGGATGAGAGGCCCTC
  CCCGTGGAGGCATGGTGCAGAAACCAGGCTTTGGCGTGGGCAGGGGGATTACAACTCCAAGGCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR216732 representing NM_133565
  Red=Cloning site Green=Tags(s)
  
  MVMEKPSPLLVGREFVRQYYTLLNQAPDMLHRFYGKNSSYAHGGLDSNGKPADAVYGQKEIHRKVMSQNF
  TNCHTKIRHVDAHATLNDGVVVQVMGLLSNNNQALRRFMQTFVLAPEGSVANKFYVHNDIFRYQDEVFGG
  FVTEPQEESEEEVEEPEERQQSPEVVADDSGTFYDQTVSNDLEEHLEEPVVEPEPEPEPEPEPEPVSDIQ
  EDKPEPALEEAAPEDVQKSASPAPADVAPAQEDLRTFSWASVTSKNLPPSGAVPVTGTPPHVVKVPASQP
  RPESKPDSQIPPQRPQRDQRAREQRINIPPQRGPRPIREAGEPGDVEPRRMVRHPDSHQLFIGNLPHEVD
  KSELKDFFQSYGNVVELRINSGGKLPNFGFVVFDDSEPVQKVLNNRPIMFRGAVRLNVEEKKTRAAREGD
  RRDNRLRGPGGPRGGPSGGMRGPPRGGMVQKPGFGVGRGITTPRQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_133565
• ORF Size: 1395 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_133565.1, NP_598249.1
• RefSeq Size: 6549 bp
• RefSeq ORF: 1398 bp
• Locus ID: 171092
• Cytogenetics: 10q22
• MW: 51.8 kDa
• Gene Summary: mouse homolog may play a role in the RNA-protein complex mediated transport of tau mRNA from neuronal cell body to the distal axon in developing neurons [RGD, Feb 2006]