G3bp1 (NM_133565) Rat Untagged Clone

CAT#: RN216732

G3bp1 (untagged) - Rat GTPase activating protein (SH3 domain) binding protein 1 (G3bp1)

Specifications

Product Data

• Type: Rat Untagged Clone
• Tag: Tag Free
• Symbol: G3bp1
• Synonyms: G3bp; RGD1310666
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  Fully Sequenced ORF

  >RN216732 representing NM_133565
  Red=Cloning site Blue=ORF Orange=Stop codon
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTTATGGAGAAGCCTAGTCCCCTGCTGGTCGGGCGGGAGTTTGTGCGACAGTACTACACCCTGCTGA
  ACCAGGCCCCGGACATGCTGCACAGATTTTATGGCAAGAACTCTTCCTATGCCCACGGGGGCTTGGATTC
  CAACGGGAAGCCAGCAGATGCAGTCTACGGGCAGAAGGAAATCCACAGGAAAGTGATGTCACAAAACTTC
  ACCAACTGCCACACCAAGATCCGCCACGTGGATGCTCACGCAACTCTGAATGACGGGGTGGTGGTCCAAG
  TGATGGGGCTGCTGTCCAACAACAACCAGGCTCTGCGGAGGTTCATGCAGACCTTTGTCCTTGCGCCTGA
  GGGCTCTGTTGCCAACAAATTCTATGTTCACAATGACATCTTCAGATACCAAGATGAGGTCTTCGGTGGC
  TTTGTCACAGAGCCTCAAGAGGAATCTGAGGAAGAAGTAGAGGAACCTGAAGAAAGACAGCAGTCACCAG
  AGGTGGTTGCTGATGACTCTGGAACTTTCTATGATCAGACTGTTAGCAATGACTTGGAGGAACATTTAGA
  GGAGCCTGTTGTGGAACCAGAACCAGAGCCGGAGCCAGAGCCGGAACCGGAACCTGTGTCCGACATTCAA
  GAGGACAAGCCTGAGCCTGCATTGGAAGAAGCCGCTCCTGAGGATGTGCAGAAGAGCGCCTCCCCGGCCC
  CGGCAGACGTGGCCCCAGCACAGGAGGACTTGAGGACATTTTCTTGGGCGTCTGTGACTAGTAAGAACCT
  TCCTCCCAGTGGGGCTGTTCCAGTGACAGGGACACCACCTCATGTGGTCAAAGTGCCAGCATCACAGCCC
  CGTCCAGAATCTAAGCCTGACTCTCAGATCCCACCACAAAGGCCTCAGAGAGATCAGAGAGCTCGAGAGC
  AGCGGATCAATATCCCTCCTCAGAGAGGACCCCGGCCAATTCGTGAGGCTGGTGAACCAGGAGATGTGGA
  ACCCCGAAGGATGGTGAGGCACCCTGACAGTCACCAGCTCTTCATTGGGAACCTGCCACATGAGGTTGAC
  AAGTCGGAACTGAAGGATTTTTTCCAAAGTTATGGGAATGTTGTGGAGCTGCGCATCAACAGTGGTGGGA
  AGTTACCCAACTTCGGTTTCGTTGTGTTTGATGACTCGGAACCAGTTCAGAAGGTCCTTAATAACAGGCC
  CATCATGTTCCGAGGTGCGGTTCGCCTGAATGTGGAAGAGAAGAAGACTCGAGCTGCCAGGGAAGGCGAC
  CGCAGGGATAACCGCCTTCGGGGACCTGGAGGCCCCCGTGGTGGGCCGAGTGGTGGGATGAGAGGCCCTC
  CCCGTGGAGGCATGGTGCAGAAACCAGGCTTTGGCGTGGGCAGGGGGATTACAACTCCAAGGCAGTGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
• Restriction Sites: SgfI-MluI
• ACCN: NM_133565
• Insert Size: 1398 bp
• OTI Disclaimer: Our molecular clone sequence data has been matched to the reference identifier above as a point of reference. Note that the complete sequence of our molecular clones may differ from the sequence published for this corresponding reference, e.g., by representing an alternative RNA splicing form or single nucleotide polymorphism (SNP).
• OTI Annotation: Clone contains native stop codon, and expresses the complete ORF without any c-terminal tag.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_133565.1, NP_598249.1
• RefSeq Size: 6549 bp
• RefSeq ORF: 1398 bp
• Locus ID: 171092
• Cytogenetics: 10q22
• Gene Summary: mouse homolog may play a role in the RNA-protein complex mediated transport of tau mRNA from neuronal cell body to the distal axon in developing neurons [RGD, Feb 2006]