Agxt (NM_001276706) Rat Tagged ORF Clone

CAT#: RR216546

Agxt (myc-DDK-tagged) - Rat alanine-glyoxylate aminotransferase (Agxt), transcript variant 1

Product Images

Circular map for RR216546

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Agxt
• Synonyms: AGT; Spat; SPT
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR216546 representing NM_001276706
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGCTCGCACCAGCTGCTGGTGCCACCCCCAGAGGCCCTGAGCAAGCCCCTGTCAATTCCTAAGAGGC
  TCCTGTTGGGTCCGGGACCCTCCAACCTGGCTCCTCGTGTGCTAGCAGCTGGAAGTCTGAGGATGATTGG
  CCACATGCAAAAAGAGATGTTTCAGATCATGGATGAGATCAAGCAGGGCATCCAGTATGTGTTCCAGACC
  AGGAACCCCCTCACACTGGTTGTCAGCGGCTCAGGACATTGTGCCATGGAGACTGCCCTGTTCAACCTCC
  TGGAGCCTGGGGACTCCTTTCTTGTGGGAACCAATGGCATCTGGGGGATACGGGCTGCAGAGATCGCTGA
  GCGGATTGGAGCCCGTGTGCACCAGATGATCAAGAAGCCTGGAGAACATTACACACTGCAGGAGGTGGAG
  GAGGGCCTGGCTCAGCATAAACCAGTGTTGCTGTTCCTGACCCACGGGGAGTCATCCACTGGTGTGCTGC
  AGCCCCTGGATGGTTTCGGGGAGCTCTGCCACAGGTATCAGTGCCTACTCCTGGTGGACTCGGTGGCATC
  ATTGGGCGGAGTCCCTATCTACATGGACCAACAAGGCATCGACATCTTGTACTCTGGCTCTCAGAAGGTC
  CTGAATGCCCCACCAGGGATCTCCCTCATCTCCTTCAACGACAAGGCCAAATCCAAAGTCTACTCCCGGA
  AGACAAAGCCAGTCTCCTTCTACACAGACATCACTTATTTGTCCAAGTTGTGGGGCTGTGAGGGCAAGAC
  CAGAGTAATTCATCATACGTTGCCTGTCATCAGCTTATACTGCCTGAGGGAGAGCCTAGCACTCATTTCA
  GAGCAGGGCCTGGAGAATTCCTGGCGGCGTCACAGGGAGGCTACAGCACATCTGCACAAGTGCCTGCGGG
  AGTTGGGCTTAAAGTTCTTTGTGAAGGACCCGGAAATCCGGCTACCTACAATCACCACCGTGACCGTGCC
  TGCCGGCTACAACTGGAGGGACATCGTCAGCTACGTGCTGGACCACTTCAACATTGAAATCTCTGGTGGT
  CTTGGGCCCTCTGAGGATAAGGTGCTGCGGATTGGCCTCCTGGGCTACAACGCCACCACAGAGAATGCGG
  ACCGTGTAGCGGAGGCCCTGAGGGAGGCCCTGCAACATTGTCCTAAGAATAAATTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR216546 representing NM_001276706
  Red=Cloning site Green=Tags(s)
  
  MGSHQLLVPPPEALSKPLSIPKRLLLGPGPSNLAPRVLAAGSLRMIGHMQKEMFQIMDEIKQGIQYVFQT
  RNPLTLVVSGSGHCAMETALFNLLEPGDSFLVGTNGIWGIRAAEIAERIGARVHQMIKKPGEHYTLQEVE
  EGLAQHKPVLLFLTHGESSTGVLQPLDGFGELCHRYQCLLLVDSVASLGGVPIYMDQQGIDILYSGSQKV
  LNAPPGISLISFNDKAKSKVYSRKTKPVSFYTDITYLSKLWGCEGKTRVIHHTLPVISLYCLRESLALIS
  EQGLENSWRRHREATAHLHKCLRELGLKFFVKDPEIRLPTITTVTVPAGYNWRDIVSYVLDHFNIEISGG
  LGPSEDKVLRIGLLGYNATTENADRVAEALREALQHCPKNKL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001276706
• ORF Size: 1176 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001276706.1, NP_001263635.1
• RefSeq Size: 1628 bp
• RefSeq ORF: 1179 bp
• Locus ID: 24792
• UniProt ID: P09139
• Cytogenetics: 9q36
• MW: 43.4 kDa
• Gene Summary: This gene encodes alanine-glyoxylate aminotransferase, which catalyzes the interconversion of L-alanine and glyoxylate to pyruvate and glycine. Two alternatively spliced transcript variants encoding distinct isoforms have been found for this gene. The longer transcript variant includes an upstream translation start codon and a downstream translation start codon. The upstream start codon initiates the translation of the mitochondrial enzyme precursor while the downstream start codon initiates the translation of the peroxisomal enzyme (see PMID:2332438). [provided by RefSeq, Feb 2013]