Sohlh1 (NM_001191852) Rat Tagged ORF Clone

CAT#: RR216465

Sohlh1 (myc-DDK-tagged) - Rat spermatogenesis and oogenesis specific basic helix-loop-helix 1 (Sohlh1)

Product Images

Circular map for RR216465

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Sohlh1
• Synonyms: RGD1564440
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR216465 representing NM_001191852
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGTCCGGTGGCCACGAGCGGGCTACTGAGGATTACGGAGTCTCCGACATTACGGGATGCAGCAAGA
  CTCCTCAGACTGAGACTCAGGAGAACTTGGAGACTCCCTCACAAAGCTCAGCCCTCTGCCCAGCTCCTGT
  GGCTGCTGCAAATTGGAGCCCCTGTCTTCGGAGAAACGTGGTCAGCGAGAGAGAACGCAGGAGGAGGATC
  TCCTTGAGCTGTGAGCGCTTGCGGGCACTACTGCCTCAGTTTGATGGCCGTCGGGAGGACATGGCTTCCG
  TCCTGGAGATGTCTGTGTGTTTCCTCCAGCTTGCCCACAGCATGGATCCTAGCTGGGAGCAGCTCTCTGT
  TCCTCAGCCTCCTCAAGAGATGTGGCACATGTGGCAGGGCGATGTTCTGCAGGTAACCCTTGCGAATCAG
  ATTGCAGACAGCAAGCCAGACTCCGGGATAGCAAAAGCATCTGCCGTGGCTCGGGTGCAGGACCCCCCAT
  GCTGTGGCATGCTGGATACAGACCAGAGCCAAGCTACTGAGAGGGCGTCGGAGCTTCTGGAGAGGCCTTC
  CTCCTGCCCTGGTAAGAGCCAGAGCTCCTTGTCATTCAGTGAGTCAGAGTCCTCCAGCTTGGGTGCTGGG
  CTCCCACCCTGGCCTCCTCACTCATGGCAGCCAGCCACTCCCGAGGCGAGTGACGCTGTTTCTGGTGGAT
  CACAGCAGGTGGGATCCCTGGCCGGGGACACTGAGTCTCCGGGCATGCTGGCTGAGGAGGCCAACTTGGT
  CTTGGCATCTGTGCCTGATGCCAGGTACACCACAGGGGCAGGGTCCGAAGTGGTGGACGGAGCATCCTTT
  CTGCTGACCACCAATCCTGATTGGTGGTTGGGGTCAGTGGAGGGCAGAGGAGGACCAGCTCTTGCCAGGA
  ACAGCCCAGTGGATGGGGCAGAGCCAATCTACATTGGAGACCCTGAGCCCTGCTCCCAGGAGCTCCAGGC
  TGGCCCCGTAGAGCTGTGGGGCTTGGATTTTGGCAGCCCTGGCCTGGCCCTGAAGGATGAAGCAGACAGC
  ATCTTCCCTGACTTTTTCCCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR216465 representing NM_001191852
  Red=Cloning site Green=Tags(s)
  
  MASGGHERATEDYGVSDITGCSKTPQTETQENLETPSQSSALCPAPVAAANWSPCLRRNVVSERERRRRI
  SLSCERLRALLPQFDGRREDMASVLEMSVCFLQLAHSMDPSWEQLSVPQPPQEMWHMWQGDVLQVTLANQ
  IADSKPDSGIAKASAVARVQDPPCCGMLDTDQSQATERASELLERPSSCPGKSQSSLSFSESESSSLGAG
  LPPWPPHSWQPATPEASDAVSGGSQQVGSLAGDTESPGMLAEEANLVLASVPDARYTTGAGSEVVDGASF
  LLTTNPDWWLGSVEGRGGPALARNSPVDGAEPIYIGDPEPCSQELQAGPVELWGLDFGSPGLALKDEADS
  IFPDFFP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001191852
• ORF Size: 1071 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001191852.1, NP_001178781.1
• RefSeq Size: 1074 bp
• RefSeq ORF: 1074 bp
• Locus ID: 362085
• Cytogenetics: 3p13
• MW: 38 kDa