Mib1 (NM_001107405) Rat Tagged ORF Clone

CAT#: RR215008

Mib1 (Myc-DDK-tagged ORF) - Rat mindbomb homolog 1 (Drosophila) (Mib1), (10 ug)

Product Images

Circular map for RR215008

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Mib1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR215008 representing NM_001107405
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGTAACTCCCGCAACAACCGAGTGATGGTGGAGGGGGTCGGCGCCCGCGTAGTGCGCGGCCCGGACT
  GGAAGTGGGGCAAACAGGACGGCGGCGAGGGCCATGTAGGCACTGTGCGGAGCTTCGAGAGCCCGGAGGA
  GGTCGTGGTGGTGTGGGACAACGGCACGGCCGCCAACTACCGCTGCTCGGGGGCCTACGACCTGCGCATC
  CTGGACAGCGCGCCCACCGGCATCAAGCATGATGGAACTATGTGTGACACCTGCCGCCAACAACCAATCA
  TTGGCATTCGATGGAAATGTGCAGAGTGCACCAATTATGATCTGTGCACTGTTTGTTATCATGGAGATAA
  ACACCACTTAAGGCATCGCTTCTATAGGATCACCACACCAGGAAGTGAACGGGTTCTGTTAGAGTCTCGT
  AGAAAATCTAAGAAGATCACAGCCAGAGGAATCTTTGCTGGTGCCAGAGTGGTGCGAGGAGTGGACTGGC
  AGTGGGAAGATCAAGACGGAGGCAATGGGCGTCGAGGAAAGGTAACGGAAATCCAGGACTGGAGTGCATC
  CAGCCCACACAGTGCAGCATATGTTCTCTGGGATAACGGTGCTAAGAACCTTTACAGGGTCGGCTTTGAG
  GGCATGTCTGATCTGAAATGTGTCCAGGATGCCAAAGGAGGTTCTTTCTACAGAGATCATTGCCCTGTGT
  TAGGTGAGCAGAATGGCAACAGGAATCCTGGTGGATTGCAGATTGGTGACCTGGTAAATATAGACCTTGA
  CCTAGAAATTGTCCAGTCTTTGCAGCACGGTCATGGAGGATGGACCGATGGCATGTTTGAGACTTTAACT
  ACAACTGGAACTGTCTGTGGCATTGATGAAGATCATGACATTGTAGTACAGTATCCAAGTGGCAATAGAT
  GGACCTTCAATCCTGCTGTTCTCACTAAGGCAAACATTGTCCGAAGTGGGGATGCTGCACAGGGTGCAGA
  AGGAGGCACCTCACAGTTTCAAGTGGGTGATCTTGTGCAAGTTTGTTATGATCTGGAAAGAATTAAACTT
  CTCCAAAGAGGACATGGTGAATGGGCTGAAGCAATGCTTCCAACTTTAGGTAAAGTGGGCAGAGTACAAC
  AGATTTATTCGGACAGTGATTTAAAGGTGGAAGTCTGTGGAACATCGTGGACATATAACCCTGCAGCAGT
  TTCCAAAGTGGCACCTGCAGGATCAACCATTAGCAATGCATCTGGTGAAAGACTCTCCCAGCTTCTGAAG
  AAATTATTTGAAACCCAAGAATCTGGTGACCTCAATGAAGAGTTGGTTAAGGCTGCTGCCAATGGGGATG
  TGGCTAAAGTGGAAGATTTGCTAAAAAGACCAGATGTGGATGTGAATGGGCAGTGCGCTGGCCACACAGC
  AATGCAAGCTGCTAGTCAAAATGGACACGTTGATATTTTGAAGTTACTTTTGAAGCAAAATGTAGATGTT
  GAAGCAGAGGATAAAGATGGAGATCGAGCAGTTCACCATGCAGCCTTTGGAGATGAGGGTGCTGTCATAG
  AAGTCCTGCACCGAGGCAGTGCTGACCTGAATGCTCGTAATAAGCGTAGACAGACGCCTCTTCATATTGC
  TGTCAATAAAGGTCATCTTCAGGTTGTGAAGACTTTGTTGGACTTTGGCTGTCATCCTAGTCTCCAGGAT
  TCTGAAGGTGACACCCCTCTCCATGATGCCATAAGTAAGAAACGGGATGACATTCTGGCGGTTCTTCTGG
  AAGCTGGAGCAGATGTGACCATCACAAACAATAATGGATTCAATGCTCTGCATCATGCTGCCCTGAGAGG
  GAATCCCAGTGCAATGCGTGTTTTACTCTCAAAATTACCAAGACCATGGATTGTGGATGAGAAGAAAGAT
  GATGGTTATACTGCCTTGCACCTGGCTGCCCTGAATAATCATGTGGAAGTGGCTGAGCTGTTGGTGCATC
  AGGGAAATGCAAACTTGGATATCCAGAACGTGAACCAGCAGACGGCCCTACACCTTGCTGTTGAACGGCA
  GCACACCCAGATTGTCAGGCTTTTGGTTCGTGCAGGTGCTAAGCTAGACATTCAGGATAAGGATGGAGAT
  ACTCCTCTGCATGAAGCTTTGAGACATCATACTTTGTCTCAACTACGTCAGCTACAAGACATGCAAGATG
  TGGGCAAGGTAGATGCTGCCTGGGAACCTTCAAAAAACACATTAATAATGGGACTTGGTACTCAGGGGGC
  CGAGAAGAAGAGTGCAGCATCTATTGCGTGTTTCTTGGCAGCCAATGGTGCCGACCTCAGCATTCGAAAT
  AAGAAGGGTCAGTCACCACTGGATCTCTGTCCTGACCCAAGTCTCTGCAAAGCTCTGGCAAAATGTCATA
  AGGAAAAAGTCAGTGGCCAAGTGGGTTCTCGGAGTCCTTCTATGATTAGCAATGATTCTGAAACCTTAGA
  GGAGTGTATGGTGTGCTCAGACATGAAGAGAGACACTCTTTTTGGTCCATGTGGACACATTGCGACGTGT
  TCTTTGTGTTCTCCTCGTGTCAAGAAATGCCTCATTTGTAAAGAACAGGTTCAGTCCAGGACGAAGATTG
  AAGAATGTGTGGTGTGTTCTGACAAGAAAGCAGCCGTTCTCTTTCAACCTTGTGGGCACATGTGTGCTTG
  TGAGAACTGTGCTAGCCTGATGAAAAAGTGTGTGCAGTGCCGGGCAGTGGTTGAGCGGAGGGTGCCTTTC
  ATCACATGCTGCGGTGGCAAGAGCTCGGAAGAGCCCTCTGATGATATCTCGAGTGGGAATATCCCAGTGC
  TGCAGAAGGACAAAGACAACACAAATGTCAATGCAGACGTGCAGAAGCTGCAGCAGCAGCTTCAGGACAT
  TAAGGAGCAGACAATGTGCCCTGTGTGCTTGGATCGCCTGAAGAACATGATTTTCCTCTGTGGCCACGGA
  ACATGCCAGCTCTGTGGAGACCGAATGAGTGAATGCCCGATTTGTCGCAAGGCAATAGAACGAAGGATTC
  TTTTGTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR215008 representing NM_001107405
  Red=Cloning site Green=Tags(s)
  
  MSNSRNNRVMVEGVGARVVRGPDWKWGKQDGGEGHVGTVRSFESPEEVVVVWDNGTAANYRCSGAYDLRI
  LDSAPTGIKHDGTMCDTCRQQPIIGIRWKCAECTNYDLCTVCYHGDKHHLRHRFYRITTPGSERVLLESR
  RKSKKITARGIFAGARVVRGVDWQWEDQDGGNGRRGKVTEIQDWSASSPHSAAYVLWDNGAKNLYRVGFE
  GMSDLKCVQDAKGGSFYRDHCPVLGEQNGNRNPGGLQIGDLVNIDLDLEIVQSLQHGHGGWTDGMFETLT
  TTGTVCGIDEDHDIVVQYPSGNRWTFNPAVLTKANIVRSGDAAQGAEGGTSQFQVGDLVQVCYDLERIKL
  LQRGHGEWAEAMLPTLGKVGRVQQIYSDSDLKVEVCGTSWTYNPAAVSKVAPAGSTISNASGERLSQLLK
  KLFETQESGDLNEELVKAAANGDVAKVEDLLKRPDVDVNGQCAGHTAMQAASQNGHVDILKLLLKQNVDV
  EAEDKDGDRAVHHAAFGDEGAVIEVLHRGSADLNARNKRRQTPLHIAVNKGHLQVVKTLLDFGCHPSLQD
  SEGDTPLHDAISKKRDDILAVLLEAGADVTITNNNGFNALHHAALRGNPSAMRVLLSKLPRPWIVDEKKD
  DGYTALHLAALNNHVEVAELLVHQGNANLDIQNVNQQTALHLAVERQHTQIVRLLVRAGAKLDIQDKDGD
  TPLHEALRHHTLSQLRQLQDMQDVGKVDAAWEPSKNTLIMGLGTQGAEKKSAASIACFLAANGADLSIRN
  KKGQSPLDLCPDPSLCKALAKCHKEKVSGQVGSRSPSMISNDSETLEECMVCSDMKRDTLFGPCGHIATC
  SLCSPRVKKCLICKEQVQSRTKIEECVVCSDKKAAVLFQPCGHMCACENCASLMKKCVQCRAVVERRVPF
  ITCCGGKSSEEPSDDISSGNIPVLQKDKDNTNVNADVQKLQQQLQDIKEQTMCPVCLDRLKNMIFLCGHG
  TCQLCGDRMSECPICRKAIERRILLY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001107405
• ORF Size: 3018 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001107405.1, NP_001100875.1
• RefSeq Size: 3796 bp
• RefSeq ORF: 3021 bp
• Locus ID: 307594
• Cytogenetics: 18p13
• MW: 110.1 kDa