Hmgcl (NM_024386) Rat Tagged ORF Clone

CAT#: RR214875

Hmgcl (Myc-DDK-tagged ORF) - Rat 3-hydroxymethyl-3-methylglutaryl-Coenzyme A lyase (Hmgcl), (10 ug)

Product Images

Circular map for RR214875

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Hmgcl
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR214875 representing NM_024386
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGACAGTGAGGAAGGCTTTCCCACAGAGGCTGGTGGGCTTGGCGTCCCTCCGGGCTGCGAGCACCT
  CCTCCATGGGCACCTTGCCGAAGCGGGTGAAGATCGTGGAAGTCGGCCCCCGAGATGGTCTGCAGAATGA
  AAAGAGTATCGTGCCGACGCCAGTGAAAATCAAACTGATAGACATGCTATCCGAAGCAGGGCTCCCGGTC
  ATCGAGGCCACCAGCTTTGTCTCTCCCAAGTGGGTGCCGCAGATGGCTGACCACTCTGACGTCTTGAAGG
  GCATTCAGAAGTTTCCCGGCATCAACTACCCGGTCCTGACACCAAACATGAAAGGCTTTGAGGAAGCGGT
  AGCTGCAGGTGCCAAGGAAGTGAGCATCTTTGGGGCTGCGTCCGAGCTCTTCACCCGGAAGAATGTGAAC
  TGCTCTATAGAGGAGAGTTTCCAGCGCTTTGATGGGGTCATGCAGGCCGCGAGGGCTGCCAGCATCTCTG
  TGAGAGGGTATGTCTCCTGTGCCCTCGGATGTCCCTACGAGGGGAAGGTCTCCCCGGCTAAAGTTGCTGA
  GGTCGCCAAGAAGTTGTACTCAATGGGCTGCTATGAGATCTCCCTTGGGGACACCATTGGCGTAGGCACG
  CCAGGACTCATGAAAGACATGCTGACTGCTGTCCTGCATGAAGTGCCTGTGGCCGCATTGGCTGTCCACT
  GCCATGACACCTATGGCCAAGCTCTGGCCAACACGTTGGTGGCCCTGCAGATGGGAGTGAGCGTTGTGGA
  CTCCTCGGTGGCAGGACTCGGAGGCTGTCCCTATGCAAAGGGGGCGTCAGGAAACTTGGCTACCGAGGAC
  CTGGTCTACATGCTGACTGGCTTAGGGATTCACACGGGTGTGAACCTCCAGAAGCTCCTAGAAGCCGGGG
  ACTTCATCTGTCAAGCCCTGAACAGAAAAACCAGTTCCAAAGTGGCACAGGCCACCTGCAAACTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR214875 representing NM_024386
  Red=Cloning site Green=Tags(s)
  
  MATVRKAFPQRLVGLASLRAASTSSMGTLPKRVKIVEVGPRDGLQNEKSIVPTPVKIKLIDMLSEAGLPV
  IEATSFVSPKWVPQMADHSDVLKGIQKFPGINYPVLTPNMKGFEEAVAAGAKEVSIFGAASELFTRKNVN
  CSIEESFQRFDGVMQAARAASISVRGYVSCALGCPYEGKVSPAKVAEVAKKLYSMGCYEISLGDTIGVGT
  PGLMKDMLTAVLHEVPVAALAVHCHDTYGQALANTLVALQMGVSVVDSSVAGLGGCPYAKGASGNLATED
  LVYMLTGLGIHTGVNLQKLLEAGDFICQALNRKTSSKVAQATCKL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_024386
• ORF Size: 975 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_024386.1, NP_077362.1
• RefSeq Size: 1390 bp
• RefSeq ORF: 978 bp
• Locus ID: 79238
• UniProt ID: P97519
• Cytogenetics: 5q36
• MW: 34.2 kDa
• Gene Summary: an enzyme of the ketogenic 3-hydroxy-3-methylglutaryl-CoA cycle [RGD, Feb 2006]