Rnf39 (NM_134374) Rat Tagged ORF Clone

CAT#: RR214664

Rnf39 (Myc-DDK-tagged ORF) - Rat ring finger protein 39 (Rnf39), (10 ug)

Product Images

Circular map for RR214664

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Rnf39
• Synonyms: Hzfw1; Lirf
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR214664 representing NM_134374
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGTTCCCGAGCTGGGCCCGGGGTTGGTGGAGCGTCTGGAGCAGCTGGCGACGTGTCCGCTGTGCG
  GGGGCCCCTTCGAGGACCCAGTCCTGCTGGCGTGCGAGCACAGCTTCTGCCGTTCGTGCTTGGCGCGCTG
  CTGGGGGTCCCCGGCGGCGCCCGGCTCGGAGGAGGCGACCCCGTCCTGCCCCTGCTGTGGTCAGCCGTGC
  CCCAGACGCAGCCTGAGGTCTAACGTGCGACTTGCAGTGGAGGTGCGCATCAGCCGCGGGCTGCGTGAGA
  AACTGGCCGAGCCCGGGGCCCGGACCGGGAGACGACGCGGCGGCCGCATCCCCACCATGGGCTGCCTGGA
  TCCCCAAGGAGAGGATATGAGAAAGACATGGAGACGATTCGATGTCCCGGTACCCAAGTCATCTAACTCA
  GAGGAGGATCTCCCTGAAGATTACCCCGTGGTAAAAAACATGCTTCACAGACTGACAGCTGACCTGACGC
  TCGACCCTCGCACTGCACATCGTGATCTGCTCATCTCCTCTGACTACCGCGGCGTGAGTCTGGCTCCACC
  CGGAACGCCTGTGCCGCTGGACAGCCCCGAGCGCTTCGATCGGCTCCGGGCTGTGTTGGGTGCGCAGGGC
  TTCGCGTCAGGTCGCCACTGCTGGGAGGTGGAGACCGCGGAAGGCGCGTGCTTCAGAGACTCTCTGGCGA
  AGGATGAGGACGCAGGAGAGAGCTGCTACGCTGTGGGCGCTGCGGGGGAGTCCGTGACGCGCAAGGGCCT
  CATCAAGCTGTGCCCATCCGAGGCTATATGGGCCGTGGAGGGCCGCGGCGGCCGCTTGTGGGCGCTCACG
  GCTCCAGAGCCCACTCTACTAGGTGGCGCCAGGCCCCCGCCGCAGCGCATTCGCGTGGACTTGGACTGGG
  AGCGGGGTCGTGTGGCCTTCTATGATGGCCGCTCATTGGACTTGCTCTTCGCCTTCCAGGCGCCGGGCCC
  GCTCGGGGAGCGTGTCTTCCCACTGCTCTGCACTTGTGACCCCCGCGCCCCACTGCGTATTGTGCCAGGA
  GAAGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR214664 representing NM_134374
  Red=Cloning site Green=Tags(s)
  
  MEVPELGPGLVERLEQLATCPLCGGPFEDPVLLACEHSFCRSCLARCWGSPAAPGSEEATPSCPCCGQPC
  PRRSLRSNVRLAVEVRISRGLREKLAEPGARTGRRRGGRIPTMGCLDPQGEDMRKTWRRFDVPVPKSSNS
  EEDLPEDYPVVKNMLHRLTADLTLDPRTAHRDLLISSDYRGVSLAPPGTPVPLDSPERFDRLRAVLGAQG
  FASGRHCWEVETAEGACFRDSLAKDEDAGESCYAVGAAGESVTRKGLIKLCPSEAIWAVEGRGGRLWALT
  APEPTLLGGARPPPQRIRVDLDWERGRVAFYDGRSLDLLFAFQAPGPLGERVFPLLCTCDPRAPLRIVPG
  EA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_134374
• ORF Size: 1056 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_134374.2, NP_599201.1
• RefSeq Size: 2001 bp
• RefSeq ORF: 1059 bp
• Locus ID: 171387
• UniProt ID: Q920M2
• Cytogenetics: 20p12
• MW: 38.3 kDa
• Gene Summary: expression is induced during long-term potentiation; may play a role in synaptic plasticity [RGD, Feb 2006]