Nr5a2 (NM_021742) Rat Tagged ORF Clone

CAT#: RR214563

Nr5a2 (Myc-DDK-tagged ORF) - Rat nuclear receptor subfamily 5, group A, member 2 (Nr5a2), (10 ug)

Product Images

Circular map for RR214563

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Nr5a2
• Synonyms: Ftf; Lrh-1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR214563 representing NM_021742
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTGCTAGTTCGATTACTGGGGATTTTCAAGACTTTCTTAAGCATGGACTTCCAGCTATTGCACCTG
  CACCAGGATCAGAGACTCCACACTCCCCCAAACTTGAGGAAAAACACCGGGAAAAACGTGCTGGGCTTCC
  GGACCGACACCGACGGCCCATTCCCGCCCGCAGCCGCCTTGTCATGCTGCCCAAAGTGGAGACGGAAGCC
  TCAGGACTGGTCCGATCGCATGGGGAACAGGGGCAGATGCCGGAAAACATGCAAGTGTCTCAATTTAAAA
  TGGTGAATTACTCCTATGATGAAGATCTGGAAGAGCTGTGTCCTGTGTGTGGCGATAAAGTATCTGGGTA
  TCATTACGGTCTCCTCACCTGTGAAAGCTGCAAGGGTTTCTTTAAGCGAACAGTCCAAAACCAGAAAAGG
  TACACGTGCATAGAGAACCAGAACTGCCAAATTGACAAGACACAGAGAAAACGATGTCCTTACTGTCGGT
  TCAAAAAATGTATCGACGTTGGAATGAAGCTGGAGGCCGTGAGAGCTGACCGCATGCGAGGAGGCAGAAA
  TAAGTTCGGGCCAATGTACAAGAGAGACAGGGCCTTGAAACAGCAGAAGAAAGCCCTCATTCGAGCCAAT
  GGACTTAAGCTGGAAGCCATGTCTCAGGTGATCCAAGCGATGCCCTCAGACCTGACCTCTGCAATTCAGA
  ACATTCATTCTGCCTCCAAAGGCCTACCTCTGAGCCATGTAGCCTTGCCTCCGACAGACTATGACAGAAG
  TCCCTTTGTCACTTCTCCCATTAGCATGACAATGCCACCTCATGGCAGTCTGCATGGTTACCAACCATAT
  GGTCACTTTCCTAATCGGGCCATCAAGTCTGAGTACCCAGACCCCTACTCCAGCTCGCCAGAGTCAATGA
  TGGGCTACTCCTACATGGATGGTTACCAGACAAGCTCCCCGGCAAGCATCCCTCATCTGATATTGGAACT
  TTTGAAGTGTGAACCCGATGAGCCTCAAGTTCAAGCGAAAATCATGGCTTACCTACAGCAAGAGCAGAAT
  AACCGGAACAGGCAGGAAAAGCTGAGCGCGTTTGGGCTCTTGTGCAAAATGGCGGACCAGACCCTGTTCT
  CCATTGTTGAGTGGGCCAGGAGTAGTATCTTCTTCAGGGAACTGAAGGTTGATGACCAAATGAAGCTGCT
  TCAGAACTGCTGGAGTGAGCTCTTGATCCTGGATCACATTTACCGGCAAGTAGCACATGGGAAGGAAGGG
  ACAATCTTCCTGGTTACCGGAGAACACGTGGACTACTCCAGCATCATCTCAAACACAGAAGTCGCATTCA
  ACAACCTCCTGAGCCTCGCCCAGGAGCTCGTGGTGAGACTCCGTTCCCTTCAGTTTGATCAGCGGGAGTT
  TGTGTGTCTGAAGTTCCTGGTGCTGTTCAGCTCAGATGTCAAGAATCTTGAGAACTTCCAGCTGGTGGAA
  GGCGTCCAAGAGCAGGTCAATGCTGCCCTGCTGGACTACACACTTTGCAACTACCCACAACAAACTGAGA
  AATTTGGACAACTGCTTCTTCGACTACCAGAAATCCGGGCAATCAGCAAACAGGCAGAAGACTACCTGTA
  CTACAAGCACGTGAATGGGGATGTGCCCTACAATAACCTTCTCATTGAAATGCTGCACGCCAAAAGAGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR214563 representing NM_021742
  Red=Cloning site Green=Tags(s)
  
  MSASSITGDFQDFLKHGLPAIAPAPGSETPHSPKLEEKHREKRAGLPDRHRRPIPARSRLVMLPKVETEA
  SGLVRSHGEQGQMPENMQVSQFKMVNYSYDEDLEELCPVCGDKVSGYHYGLLTCESCKGFFKRTVQNQKR
  YTCIENQNCQIDKTQRKRCPYCRFKKCIDVGMKLEAVRADRMRGGRNKFGPMYKRDRALKQQKKALIRAN
  GLKLEAMSQVIQAMPSDLTSAIQNIHSASKGLPLSHVALPPTDYDRSPFVTSPISMTMPPHGSLHGYQPY
  GHFPNRAIKSEYPDPYSSSPESMMGYSYMDGYQTSSPASIPHLILELLKCEPDEPQVQAKIMAYLQQEQN
  NRNRQEKLSAFGLLCKMADQTLFSIVEWARSSIFFRELKVDDQMKLLQNCWSELLILDHIYRQVAHGKEG
  TIFLVTGEHVDYSSIISNTEVAFNNLLSLAQELVVRLRSLQFDQREFVCLKFLVLFSSDVKNLENFQLVE
  GVQEQVNAALLDYTLCNYPQQTEKFGQLLLRLPEIRAISKQAEDYLYYKHVNGDVPYNNLLIEMLHAKRA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021742
• ORF Size: 1680 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021742.1, NP_068510.1
• RefSeq Size: 1931 bp
• RefSeq ORF: 1683 bp
• Locus ID: 60349
• UniProt ID: Q9QWM1
• Cytogenetics: 13q13
• MW: 63.9 kDa
• Gene Summary: an orphan nuclear receptor that may bind DNA and activate gene transcription [RGD, Feb 2006]