Rtn4 (NM_031831) Rat Tagged ORF Clone

CAT#: RR214501

Rtn4 (Myc-DDK-tagged ORF) - Rat reticulon 4 (Rtn4), (10 ug)

Product Images

Circular map for RR214501

Specifications

Product Data

• Type: Rat Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: Rtn4
• Synonyms: NI-250; Nogo; Nogo-A; r; rat N; rat NogoA; Vp20
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RR214501 representing NM_031831
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAGACATAGACCAGTCGTCGCTGGTCTCCTCGTCCACGGACAGCCCGCCCCGGCCTCCGCCCGCCT
  TCAAGTACCAGTTCGTGACGGAGCCCGAGGACGAGGAGGACGAGGAGGAGGAGGAGGACGAGGAGGAGGA
  CGACGAGGACCTAGAGGAACTGGAGGTGCTGGAGAGGAAGCCCGCAGCCGGGCTGTCCGCAGCTGCGGTG
  CCGCCCGCCGCCGCCGCGCCGCTGCTGGACTTCAGCAGCGACTCGGTGCCCCCCGCGCCCCGCGGGCCGC
  TGCCGGCCGCGCCCCCTGCCGCTCCTGAGAGGCAGCCATCCTGGGAACGCAGCCCCGCGGCGCCCGCGCC
  ATCCCTGCCGCCCGCTGCCGCAGTCCTGCCCTCCAAGCTCCCAGAGGACGACGAGCCTCCGGCGAGGCCC
  CCGCCTCCGCCGCCAGCCGGCGCGAGCCCCCTGGCGGAGCCCGCCGCGCCCCCTTCCACGCCGGCCGCGC
  CCAAGCGCAGGGGCTCCGGCTCAGTGGATGAGACCCTTTTTGCTCTTCCTGCTGCATCTGAGCCTGTGAT
  ACCCTCCTCTGCAGAAAAAATTATGGATTTGATGGAGCAGCCAGGTAACACTGTTTCGTCTGGTCAAGAG
  GATTTCCCATCTGTCCTGCTTGAAACTGCTGCCTCTCTTCCTTCTCTATCTCCTCTCTCAACTGTTTCTT
  TTAAAGAACATGGATACCTTGGTAACTTATCAGCAGTGTCATCCTCAGAAGGAACAATTGAAGAAACTTT
  AAATGAAGCTTCTAAAGAGTTGCCAGAGAGGGCAACAAATCCATTTGTAAATAGAGATTTAGCAGAATTT
  TCAGAATTAGAATATTCAGAAATGGGATCATCTTTTAAAGGCTCCCCAAAAGGAGAGTCAGCCATATTAG
  TAGAAAACACTAAGGAAGAAGTAATTGTGAGGAGTAAAGACAAAGAGGATTTAGTTTGTAGTGCAGCCCT
  TCACAGTCCACAAGAATCACCTGTGGGTAAAGAAGACAGAGTTGTGTCTCCAGAAAAGACAATGGACATT
  TTTAATGAAATGCAGATGTCAGTAGTAGCACCTGTGAGGGAAGAGTATGCAGACTTTAAGCCATTTGAAC
  AAGCATGGGAAGTGAAAGATACTTATGAGGGAAGTAGGGATGTGCTGGCTGCTAGAGCTAATGTGGAAAG
  TAAAGTGGACAGAAAATGCTTGGAAGATAGCCTGGAGCAAAAAAGTCTTGGGAAGGATAGTGAAGGCAGA
  AATGAGGATGCTTCTTTCCCCAGTACCCCAGAACCTGTGAAGGACAGCTCCAGAGCATATATTACCTGTG
  CTTCCTTTACCTCAGCAACCGAAAGCACCACAGCAAACACTTTCCCTTTGTTAGAAGATCATACTTCAGA
  AAATAAAACAGATGAAAAAAAAATAGAAGAAAGGAAGGCCCAAATTATAACAGAGAAGACTAGCCCCAAA
  ACGTCAAATCCTTTCCTTGTAGCAGTACAGGATTCTGAGGCAGATTATGTTACAACAGATACCTTATCAA
  AGGTGACTGAGGCAGCAGTGTCAAACATGCCTGAAGGTCTGACGCCAGATTTAGTTCAGGAAGCATGTGA
  AAGTGAACTGAATGAAGCCACAGGTACAAAGATTGCTTATGAAACAAAAGTGGACTTGGTCCAAACATCA
  GAAGCTATACAAGAATCACTTTACCCCACAGCACAGCTTTGCCCATCATTTGAGGAAGCTGAAGCAACTC
  CGTCACCAGTTTTGCCTGATATTGTTATGGAAGCACCATTAAATTCTCTCCTTCCAAGCGCTGGTGCTTC
  TGTAGTGCAGCCCAGTGTATCCCCACTGGAAGCACCTCCTCCAGTTAGTTATGACAGTATAAAGCTTGAG
  CCTGAAAACCCCCCACCATATGAAGAAGCCATGAATGTAGCACTAAAAGCTTTGGGAACAAAGGAAGGAA
  TAAAAGAGCCTGAAAGTTTTAATGCAGCTGTTCAGGAAACAGAAGCTCCTTATATATCCATTGCGTGTGA
  TTTAATTAAAGAAACAAAGCTCTCCACTGAGCCAAGTCCAGATTTCTCTAATTATTCAGAAATAGCAAAA
  TTCGAGAAGTCGGTGCCCGAACACGCTGAGCTAGTGGAGGATTCCTCACCTGAATCTGAACCAGTTGACT
  TATTTAGTGATGATTCGATTCCTGAAGTCCCACAAACACAAGAGGAGGCTGTGATGCTCATGAAGGAGAG
  TCTCACTGAAGTGTCTGAGACAGTAGCCCAGCACAAAGAGGAGAGACTTAGTGCCTCACCTCAGGAGCTA
  GGAAAGCCATATTTAGAGTCTTTTCAGCCCAATTTACATAGTACAAAAGATGCTGCATCTAATGACATTC
  CAACATTGACCAAAAAGGAGAAAATTTCTTTGCAAATGGAAGAGTTTAATACTGCAATTTATTCAAATGA
  TGACTTACTTTCTTCTAAGGAAGACAAAATAAAAGAAAGTGAAACATTTTCAGATTCATCTCCGATTGAG
  ATAATAGATGAATTTCCCACGTTTGTCAGTGCTAAAGATGATTCTCCTAAATTAGCCAAGGAGTACACTG
  ATCTAGAAGTATCCGACAAAAGTGAAATTGCTAATATCCAAAGCGGGGCAGATTCATTGCCTTGCTTAGA
  ATTGCCCTGTGACCTTTCTTTCAAGAATATATATCCTAAAGATGAAGTACATGTTTCAGATGAATTCTCC
  GAAAATAGGTCCAGTGTATCTAAGGCATCCATATCGCCTTCAAATGTCTCTGCTTTGGAACCTCAGACAG
  AAATGGGCAGCATAGTTAAATCCAAATCACTTACGAAAGAAGCAGAGAAAAAACTTCCTTCTGACACAGA
  GAAAGAGGACAGATCCCTGTCAGCTGTATTGTCAGCAGAGCTGAGTAAAACTTCAGTTGTTGACCTCCTC
  TACTGGAGAGACATTAAGAAGACTGGAGTGGTGTTTGGTGCCAGCTTATTCCTGCTGCTGTCTCTGACAG
  TGTTCAGCATTGTCAGTGTAACGGCCTACATTGCCTTGGCCCTGCTCTCGGTGACTATCAGCTTTAGGAT
  ATATAAGGGCGTGATCCAGGCTATCCAGAAATCAGATGAAGGCCACCCATTCAGGGCATATTTAGAATCT
  GAAGTTGCTATATCAGAGGAATTGGTTCAGAAATACAGTAATTCTGCTCTTGGTCATGTGAACAGCACAA
  TAAAAGAACTGAGGCGGCTTTTCTTAGTTGATGATTTAGTTGATTCCCTGAAGTTTGCAGTGTTGATGTG
  GGTGTTTACTTATGTTGGTGCCTTGTTCAATGGTCTGACACTACTGATTTTAGCTCTGATCTCACTCTTC
  AGTATTCCTGTTATTTATGAACGGCATCAGGTGCAGATAGATCATTATCTAGGACTTGCAAACAAGAGTG
  TTAAGGATGCCATGGCCAAAATCCAAGCAAAAATCCCTGGATTGAAGCGCAAAGCAGAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RR214501 representing NM_031831
  Red=Cloning site Green=Tags(s)
  
  MEDIDQSSLVSSSTDSPPRPPPAFKYQFVTEPEDEEDEEEEEDEEEDDEDLEELEVLERKPAAGLSAAAV
  PPAAAAPLLDFSSDSVPPAPRGPLPAAPPAAPERQPSWERSPAAPAPSLPPAAAVLPSKLPEDDEPPARP
  PPPPPAGASPLAEPAAPPSTPAAPKRRGSGSVDETLFALPAASEPVIPSSAEKIMDLMEQPGNTVSSGQE
  DFPSVLLETAASLPSLSPLSTVSFKEHGYLGNLSAVSSSEGTIEETLNEASKELPERATNPFVNRDLAEF
  SELEYSEMGSSFKGSPKGESAILVENTKEEVIVRSKDKEDLVCSAALHSPQESPVGKEDRVVSPEKTMDI
  FNEMQMSVVAPVREEYADFKPFEQAWEVKDTYEGSRDVLAARANVESKVDRKCLEDSLEQKSLGKDSEGR
  NEDASFPSTPEPVKDSSRAYITCASFTSATESTTANTFPLLEDHTSENKTDEKKIEERKAQIITEKTSPK
  TSNPFLVAVQDSEADYVTTDTLSKVTEAAVSNMPEGLTPDLVQEACESELNEATGTKIAYETKVDLVQTS
  EAIQESLYPTAQLCPSFEEAEATPSPVLPDIVMEAPLNSLLPSAGASVVQPSVSPLEAPPPVSYDSIKLE
  PENPPPYEEAMNVALKALGTKEGIKEPESFNAAVQETEAPYISIACDLIKETKLSTEPSPDFSNYSEIAK
  FEKSVPEHAELVEDSSPESEPVDLFSDDSIPEVPQTQEEAVMLMKESLTEVSETVAQHKEERLSASPQEL
  GKPYLESFQPNLHSTKDAASNDIPTLTKKEKISLQMEEFNTAIYSNDDLLSSKEDKIKESETFSDSSPIE
  IIDEFPTFVSAKDDSPKLAKEYTDLEVSDKSEIANIQSGADSLPCLELPCDLSFKNIYPKDEVHVSDEFS
  ENRSSVSKASISPSNVSALEPQTEMGSIVKSKSLTKEAEKKLPSDTEKEDRSLSAVLSAELSKTSVVDLL
  YWRDIKKTGVVFGASLFLLLSLTVFSIVSVTAYIALALLSVTISFRIYKGVIQAIQKSDEGHPFRAYLES
  EVAISEELVQKYSNSALGHVNSTIKELRRLFLVDDLVDSLKFAVLMWVFTYVGALFNGLTLLILALISLF
  SIPVIYERHQVQIDHYLGLANKSVKDAMAKIQAKIPGLKRKAD
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031831
• ORF Size: 3489 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031831.1, NP_114019.1
• RefSeq Size: 4684 bp
• RefSeq ORF: 3492 bp
• Locus ID: 83765
• UniProt ID: Q9JK11
• Cytogenetics: 14q22
• MW: 126.4 kDa
• Gene Summary: a myelin protein that is a potent inhibitor of neurite growth [RGD, Feb 2006]